As a promising concept, microfluidic paper-based analytical devices (μPADs) have seen rapid development in recent years. In this study, a new method of fabricating μPADs by atom stamp printing (ASP) is proposed and studied. The advantages of this new method compared to other methods include its low cost, ease of operation, high production efficiency, and high resolution (the minimum widths of the hydrophilic channels and hydrophobic barriers are 328 and 312 μm, respectively). As a proof of concept, μPADs fabricated with the ASP method were used to detect different concentrations of Cu²⁺via a colorimetric method. Moreover, combined with a distance-based detection method, these devices achieved a Cu²⁺detection limit of down to 1 mg/L. In addition, a new paper-based solid–liquid extraction device (PSED) based on a three-dimensional (3D) μPAD with a “3 + 2” structure and a recyclable extraction mode was developed. Specifically, using the characteristics of paper filtration and capillary force, the device completed multiple extraction and filtration steps from traditional solid–liquid extraction processes with high efficiency. The developed PSED platform allows the detection of heavy metal ions much more cheaply and simply and with a faster response time at the point of care, and it has great promise for applications in food safety and environmental pollution in resource-limited areas.

Flexible and wearable electronic sensors hold great promise for improving the quality of life, especially in the field of healthcare monitoring, owing to their low cost, flexibility, high electromechanical coupling performance, high sensitivity, and biocompatibility. To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements. The flexible films made using the polarization process are tested. It was found that the maximum piezoelectric coefficient was 31 pC N⁻¹ and the maximum tensile force of the flexible films was 26 N. A wide range of bending angles between 15° and 180° proves the flexibility capability of the films. In addition, the charge density shows a proportional relation with the applied mechanical force, and it could sense stress of 1 kPa. Finally, plantar pressure sensors are arranged and packaged with a film array followed by connection with the detection module. Then, the pressure curves of each point on the plantar are obtained. Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed. Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.

Platelet separation and purification are required in many applications including in the detection and treatment of hemorrhagic and thrombotic diseases, in addition to transfusions and in medical research. In this study, platelet separation was evaluated using a novel zigzag microchannel fluidic device while leveraging a dielectrophoresis (DEP) electric field using the COMSOL multiphysics software package and additional experimentation. The zigzag-shaped microchannel was superior to straight channel devices for cell separation because the sharp corners reduced the required horizontal distance needed for separation and also contributed to an asymmetric DEP electric field. A perfect linear relationship was observed between the separation distance and the corner angles. A quadratic relationship (R² = 0.99) was observed between the driving voltage and the width and the lengths of the channel, allowing for optimization of these properties. In addition, the voltage was inversely proportional to the channel width and proportional to the channel length. An optimal velocity ratio of 1:4 was identified for the velocities of the two device inlets. The proposed device was fabricated using laser engraving and lithography with optimized structures including a 0.5 mm channel width, a 120° corner angle, a 0.3 mm channel depth, and a 17 mm channel length. A separation efficiency of 99.4% was achieved using a voltage of 20 V and a velocity ratio of 1:4. The easy fabrication, lower required voltage, label-free detection, high efficiency, and environmental friendliness of this device make it suitable for point-of-care medicine and biological applications. Moreover, it can be used for the separation of other types of compounds including lipids.