Herein, we report a drug eluting scaffold composed of a composite nanofibers of poly(ε-caprolactone) (PCL) and poly(glycerol sebacate) (PGS) loaded with Hydroxyapatite nanoparticles (HANPs) and simvastatin (SIM) mimicking the bone extracellular matrix (ECM) to improve bone cell proliferation and regeneration process. Indeed, the addition of PGS results in a slight increase in the average fiber diameter compared to PCL. However, the presence of HANPs in the composite nanofibers induced a greater fiber diameter distribution, without significantly changing the average fiber diameter. The in vitro drug release result revealed that the sustained release of SIM from the composite nanofiber obeying the Korsemeyer–Peppas and Kpocha models revealing a non-Fickian diffusion mechanism and the release mechanism follows diffusion rather than polymer erosion. Biomineralization assessment of the nanofibers was carried out in simulated body fluid (SBF). SEM and EDS analysis confirmed nucleation of the hydroxyapatite layer on the surface of the composite nanofibers mimicking the natural apatite layer. Moreover, in vitro studies revealed that the PCL-PGS-HA displayed better cell proliferation and adhesion compared to the control sample, hence improving the regeneration process. This suggests that the fabricated PCL-PGS-HA could be a promising future scaffold for control drug delivery and bone tissue regeneration application.

The aim of this study is to develop a novel functional bi-layered membrane loaded titanium oxide (TiO₂) and tetracycline (TTC) for application in wound dressing. The advantages of the electrospinning technique have to be considered for the uniform distribution of nanoparticles and TTC drug. The as prepared nanofibers and TiO₂ were characterized in terms of morphology, fiber diameter, mechanical properties and surface wettability. The in vitro drug release study revealed initial burst release followed by a sustained control release of TTC for 4 days. The in vitro antibacterial of the bi-layered nanofibers was conducted against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria species showing excellent antibacterial effect for drug loaded samples compared with PCL nanofibers. Subsequently, cell counting kit-8 (CCK-8) and confocal laser scanning microscopy (CLSM) were used to evaluate its biocompatibility in vitro. Our results revealed that the bi-layered membrane has better antibacterial and cell compatibility than the control fiber. This suggests that the fabricated biocompatible scaffold is appropriate for a variety of wound dressing applications.