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23Years (2004-Present)
(Calculated once Scilit publication data is synced: current year − year of first publication + 1)
Representative Publications
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Journal Article
MDMartin Doležal
Martin DoležalEJEduard Jirkovský
Eduard Jirkovský
Kateřina MacákováLKLenka Kujovská Krčmová
Lenka Kujovská KrčmováLJLenka Javorská
Lenka JavorskáOOOn Behalf Of The Oemonom
On Behalf Of The Oemonom
+5
Vitamin C (L-ascorbic acid) has been known as an antioxidant for most people. However, its physiological role is much larger and encompasses very different processes ranging from facilitation of iron absorption through involvement in hormones and carnitine synthesis for important roles in epigenetic processes. Contrarily, high doses act as a pro-oxidant than an anti-oxidant. This may also be the reason why plasma levels are meticulously regulated on the level of absorption and excretion in the kidney. Interestingly, most cells contain vitamin C in millimolar concentrations, which is much higher than its plasma concentrations, and compared to other vitamins. The role of vitamin C is well demonstrated by miscellaneous symptoms of its absence—scurvy. The only clinically well-documented indication for vitamin C is scurvy. The effects of vitamin C administration on cancer, cardiovascular diseases, and infections are rather minor or even debatable in the general population. Vitamin C is relatively safe, but caution should be given to the administration of high doses, which can cause overt side effects in some susceptible patients (e.g., oxalate renal stones). Lastly, analytical methods for its determination with advantages and pitfalls are also discussed in this review.
Journal Article
ACAlejandro Carazo
Alejandro CarazoKateřina MacákováKMKateřina Matoušová
Kateřina MatoušováLKLenka Kujovská Krčmová
Lenka Kujovská Krčmová
Michele Protti
Premysl MladenkaVitamin A is a group of vital micronutrients widely present in the human diet. Animal-based products are a rich source of the retinyl ester form of the vitamin, while vegetables and fruits contain carotenoids, most of which are provitamin A. Vitamin A plays a key role in the correct functioning of multiple physiological functions. The human organism can metabolize natural forms of vitamin A and provitamin A into biologically active forms (retinol, retinal, retinoic acid), which interact with multiple molecular targets, including nuclear receptors, opsin in the retina and, according to the latest research, also some enzymes. In this review, we aim to provide a complex view on the present knowledge about vitamin A ranging from its sources through its physiological functions to consequences of its deficiency and metabolic fate up to possible pharmacological administration and potential toxicity. Current analytical methods used for its detection in real samples are included as well.
Journal Article
MHMarcel Hrubša
Marcel HrubšaTSTomas Siatka
Tomas SiatkaINIveta Nejmanová
Iveta NejmanováMVMarie Vopršalová
Marie VopršalováLKLenka Kujovská Krčmová
Lenka Kujovská KrčmováOOOn Behalf Of The Oemonom
On Behalf Of The Oemonom
+7
This review summarizes the current knowledge on essential vitamins B1, B2, B3, and B5. These B-complex vitamins must be taken from diet, with the exception of vitamin B3, that can also be synthetized from amino acid tryptophan. All of these vitamins are water soluble, which determines their main properties, namely: they are partly lost when food is washed or boiled since they migrate to the water; the requirement of membrane transporters for their permeation into the cells; and their safety since any excess is rapidly eliminated via the kidney. The therapeutic use of B-complex vitamins is mostly limited to hypovitaminoses or similar conditions, but, as they are generally very safe, they have also been examined in other pathological conditions. Nicotinic acid, a form of vitamin B3, is the only exception because it is a known hypolipidemic agent in gram doses. The article also sums up: (i) the current methods for detection of the vitamins of the B-complex in biological fluids; (ii) the food and other sources of these vitamins including the effect of common processing and storage methods on their content; and (iii) their physiological function.
2 September 2021 in Nutrition Reviews
Journal Article
Premysl MladenkaKMKateřina Macáková
Kateřina Macáková
LKLenka Kujovská Krčmová
Lenka Kujovská Krčmová
LJLenka Javorská
Lenka Javorská
KMKristýna Mrštná
Kristýna Mrštná
TCThe Oemonom Researchers And Collaborators
The Oemonom Researchers And Collaborators
+4
Vitamin K is traditionally connected with blood coagulation, since it is needed for the posttranslational modification of 7 proteins involved in this cascade. However, it is also involved in the maturation of another 11 or 12 proteins that play different roles, encompassing in particular the modulation of the calcification of connective tissues. Since this process is physiologically needed in bones, but is pathological in arteries, a great deal of research has been devoted to finding a possible link between vitamin K and the prevention of osteoporosis and cardiovascular diseases. Unfortunately, the current knowledge does not allow us to make a decisive conclusion about such a link. One possible explanation for this is the diversity of the biological activity of vitamin K, which is not a single compound but a general term covering natural plant and animal forms of vitamin K (K1 and K2) as well as their synthetic congeners (K3 and K4). Vitamin K1 (phylloquinone) is found in several vegetables. Menaquinones (MK4–MK13, a series of compounds known as vitamin K2) are mostly of a bacterial origin and are introduced into the human diet mainly through fermented cheeses. Current knowledge about the kinetics of different forms of vitamin K, their detection, and their toxicity are discussed in this review.
16 May 2022 in Critical Reviews in Clinical Laboratory Sciences
Journal Article
JJJiri Janousek
Jiri JanousekVPVeronika Pilařová
Veronika PilařováKateřina MacákováANAnderson Nomura
Anderson Nomura
JVJéssica Veiga-Matos
Jéssica Veiga-Matos
Premysl Mladenka +6
Vitamin D has a well-known role in the calcium homeostasis associated with the maintenance of healthy bones. It increases the efficiency of the intestinal absorption of dietary calcium, reduces calcium losses in urine, and mobilizes calcium stored in the skeleton. However, vitamin D receptors are present ubiquitously in the human body and indeed, vitamin D has a plethora of non-calcemic functions. In contrast to most vitamins, sufficient vitamin D can be synthesized in human skin. However, its production can be markedly decreased due to factors such as clothing, sunscreens, intentional avoidance of the direct sunlight, or the high latitude of the residence. Indeed, more than one billion people worldwide are vitamin D deficient, and the deficiency is frequently undiagnosed. The chronic deficiency is not only associated with rickets/osteomalacia/osteoporosis but it is also linked to a higher risk of hypertension, type 1 diabetes, multiple sclerosis, or cancer. Supplementation of vitamin D may be hence beneficial, but the intake of vitamin D should be under the supervision of health professionals because overdosing leads to intoxication with severe health consequences. For monitoring vitamin D, several analytical methods are employed, and their advantages and disadvantages are discussed in detail in this review.
1 May 2011 in Journal of Inorganic Biochemistry
Journal Article
Premysl MladenkaKateřina MacákováTFTomáš Filipský
Tomáš Filipský
LZLibuše Zatloukalová
Libuše Zatloukalová
LJLuděk Jahodář
Luděk Jahodář
Luciano Saso +3
About Me
Přemysl Mladěnka, Pharm.D., Ph.D. is a full professor of human and veterinary pharmacology and the head of the group studying cardiovascular and respiratory pharmacology and toxicology at the Department of Pharmacology and Toxicology, Faculty of Pharmacy, Charles University (FP CU), Czech Republic.
Education: Received MSc/PharmD. in pharmacy in 2003/2009 and Ph.D. in pharmacology and toxicology in 2008. Since 2014, associate professor of human and veterinary pharmacology and since 2020 full professor at the FP CU.
Research: Primary research focus encompasses cardiovascular pharmacology and toxicology of both natural and synthetic drugs with emphasis on their pharmacokinetics.
Publication record: Author of 100 peer-reviewed publications (total IF ˃ 370, average IF in impacted publications: 3.9), which have been cited (without self-citations) more than 1600 times (H-index 21, both Scopus without self-citations).
Selected awards: Awardee of the Minister of the Czech Republic Education prize, Youth and Sports for remarkable results during doctoral study (2009); the Young Scientist Award of the Danone Institution for the best work in the field of nutrition (2011); selected for participation in the 64th Lindau Nobel Laureate Meeting (2014); and poster award for extraordinary scientific achievements in the field (annual meeting of The Society for Free Radical Research—Europe 2014).