This page has only limited features, please log in for full access.

Unclaimed
A.E. Colville
School of Life Sciences, University of Technology Sydney, Ultimo, Australia

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 21 October 2020 in Ecotoxicology and Environmental Safety
Reads 0
Downloads 0

In order to study the toxicity of the cyanobacterial non-protein amino acids (NPAAs) L-β-N-methylamino-L-alanine (BMAA) and its structural isomer L-2,4-diaminobutyric acid (DAB) in the forage crop plant alfalfa (Medicago sativa), seedlings were exposed to NPAA-containing media for four days. Root growth was significantly inhibited by both treatments. The content of derivatised free and protein-bound BMAA and DAB in seedlings was then analysed by LC-MS/MS. Both NPAAs were detected in free and protein-bound fractions with higher levels detected in free fractions. Compared to shoots, there was approximately tenfold more BMAA and DAB in alfalfa roots. These results suggest that NPAAs might be taken up into crop plants from contaminated irrigation water and enter the food chain. This may present an exposure pathway for NPAAs in humans.

ACS Style

Kate Samardzic; Joel R. Steele; Jake P. Violi; Anne Colville; Simon M. Mitrovic; Kenneth J. Rodgers. Toxicity and bioaccumulation of two non-protein amino acids synthesised by cyanobacteria, β-N-Methylamino-L-alanine (BMAA) and 2,4-diaminobutyric acid (DAB), on a crop plant. Ecotoxicology and Environmental Safety 2020, 208, 111515 .

AMA Style

Kate Samardzic, Joel R. Steele, Jake P. Violi, Anne Colville, Simon M. Mitrovic, Kenneth J. Rodgers. Toxicity and bioaccumulation of two non-protein amino acids synthesised by cyanobacteria, β-N-Methylamino-L-alanine (BMAA) and 2,4-diaminobutyric acid (DAB), on a crop plant. Ecotoxicology and Environmental Safety. 2020; 208 ():111515.

Chicago/Turabian Style

Kate Samardzic; Joel R. Steele; Jake P. Violi; Anne Colville; Simon M. Mitrovic; Kenneth J. Rodgers. 2020. "Toxicity and bioaccumulation of two non-protein amino acids synthesised by cyanobacteria, β-N-Methylamino-L-alanine (BMAA) and 2,4-diaminobutyric acid (DAB), on a crop plant." Ecotoxicology and Environmental Safety 208, no. : 111515.

Journal article
Published: 02 September 2019 in Toxins
Reads 0
Downloads 0

β-methylamino-L-alanine (BMAA) is a non-protein amino acid that has been implicated as a risk factor for motor neurone disease (MND). BMAA is produced by a wide range of cyanobacteria globally and by a small number of marine diatoms. BMAA is commonly found with two of its constitutional isomers: 2,4-diaminobutyric acid (2,4-DAB), and N-(2-aminoethyl)glycine (AEG). The isomer 2,4-DAB, like BMAA, has neurotoxic properties. While many studies have shown BMAA production by cyanobacteria, few studies have looked at other algal groups. Several studies have shown BMAA production by marine diatoms; however, there are no studies examining freshwater diatoms. This study aimed to determine if some freshwater diatoms produced BMAA, and which diatom taxa are capable of BMAA, 2,4-DAB and AEG production. Five axenic diatom cultures were established from river and lake sites across eastern Australia. Cultures were harvested during the stationary growth phase and intracellular amino acids were extracted. Using liquid chromatography triple quadrupole mass spectrometry (LC-MS/MS), diatom extracts were analysed for the presence of both free and protein-associated BMAA, 2,4-DAB and AEG. Of the five diatom cultures analysed, four were found to have detectable BMAA and AEG, while 2,4-DAB was found in all cultures. These results show that BMAA production by diatoms is not confined to marine genera and that the prevalence of these non-protein amino acids in Australian freshwater environments cannot be solely attributed to cyanobacteria.

ACS Style

Jake P. Violi; Jordan A. Facey; Simon M. Mitrovic; Anne Colville; Kenneth J. Rodgers. Production of β-methylamino-L-alanine (BMAA) and Its Isomers by Freshwater Diatoms. Toxins 2019, 11, 512 .

AMA Style

Jake P. Violi, Jordan A. Facey, Simon M. Mitrovic, Anne Colville, Kenneth J. Rodgers. Production of β-methylamino-L-alanine (BMAA) and Its Isomers by Freshwater Diatoms. Toxins. 2019; 11 (9):512.

Chicago/Turabian Style

Jake P. Violi; Jordan A. Facey; Simon M. Mitrovic; Anne Colville; Kenneth J. Rodgers. 2019. "Production of β-methylamino-L-alanine (BMAA) and Its Isomers by Freshwater Diatoms." Toxins 11, no. 9: 512.

Journal article
Published: 01 May 2019 in Ecotoxicology and Environmental Safety
Reads 0
Downloads 0

Environmental exposure to the amino acid β-methylamino-L-alanine (BMAA) was linked to the high incidence of neurodegenerative disease first reported on the island of Guam in the 1940s and has more recently been implicated in an increased incidence of amyotrophic lateral sclerosis (ALS) in parts of the USA. BMAA has been shown to be produced by a range of cyanobacteria and some marine diatoms and dinoflagellates in different parts of the world. BMAA is commonly found with two of its constitutional isomers: 2,4- diaminobutyric acid (2,4-DAB) and N-(2-aminoethyl) glycine (AEG). These isomers are thought to be co-produced by the same organisms that produce BMAA and MS/MS analysis following LC separation can add an additional level of specificity over LC-FL. Although the presence of BMAA and 2,4-DAB in surface scum samples from several sites in Australia has been reported, which Australian cyanobacterial species are capable of BMAA, 2,4-DAB and AEG production remains unknown. The aims of the present studies were to identify some of the cyanobacterial genera or species that can produce BMAA, 2,4-DAB and AEG in freshwater cyanobacteria blooms in eastern Australia. Eleven freshwater sites were sampled and from these, 19 single-species cyanobacterial cultures were established. Amino acids were extracted from cyanobacterial cultures and analysed using liquid chromatography-tandem mass spectrometry. BMAA was detected in 17 of the 19 isolates, 2,4-DAB was detected in all isolates, and AEG was detected in 18 of the 19 isolates, showing the prevalence of these amino acids in Australian freshwater cyanobacteria. Concentrations of all three isomers in Australian cyanobacteria were generally higher than the concentrations reported elsewhere. This study confirmed the presence of BMAA and its isomers in cyanobacteria isolated from eastern Australian freshwater systems, and determined which Australian cyanobacterial genera or species were capable of producing them when cultured under laboratory conditions.

ACS Style

Jake P. Violi; Simon Mitrovic; Anne Colville; Brendan J. Main; Kenneth J. Rodgers. Prevalence of β-methylamino-L-alanine (BMAA) and its isomers in freshwater cyanobacteria isolated from eastern Australia. Ecotoxicology and Environmental Safety 2019, 172, 72 -81.

AMA Style

Jake P. Violi, Simon Mitrovic, Anne Colville, Brendan J. Main, Kenneth J. Rodgers. Prevalence of β-methylamino-L-alanine (BMAA) and its isomers in freshwater cyanobacteria isolated from eastern Australia. Ecotoxicology and Environmental Safety. 2019; 172 ():72-81.

Chicago/Turabian Style

Jake P. Violi; Simon Mitrovic; Anne Colville; Brendan J. Main; Kenneth J. Rodgers. 2019. "Prevalence of β-methylamino-L-alanine (BMAA) and its isomers in freshwater cyanobacteria isolated from eastern Australia." Ecotoxicology and Environmental Safety 172, no. : 72-81.

Journal article
Published: 01 September 2012 in Ecotoxicology and Environmental Safety
Reads 0
Downloads 0

Because of pressure on water supplies world-wide, there is increasing interest in methods of remediating contaminated ground waters. However, with some remediation processes, the breakdown products are more toxic than the original contaminant. Organic matter and salinity may also influence degradation efficiency. This study tested the efficiency of Fenton oxidation in degrading the sulfonylurea herbicide metsulfuron methyl (MeS), and tested the reaction products for phytotoxicity with the Lemna (duckweed) bioassay. The efficiency of degradation by Fenton's reagent (Fe(2+)=0.09 mM; H(2)O(2)=1.76 mM, 4h) decreased with increasing initial MeS concentration, from 98% with 5 mg/L MeS, to 63% with 70 mg/L MeS. Addition of NaCl (10mM) and organic matter (humic acid at 0.2 and 2.0mg C/L as Total Organic Carbon) reduced the efficiency of degradation at low initial MeS concentrations (5 and 10 mg/L), but had no effect at high concentrations. The residual Fenton's reagent after Fenton's oxidation was toxic to Lemna. After removal of residual iron and H(2)O(2), the measured toxicity to Lemna in the treated samples could be explained by the concentrations of MeS as measured by HPLC/UV detection, so there was no evidence of additional toxicity or amelioration due to the by-products or formulation materials.

ACS Style

Javeed M. Abdul; Anne Colville; Richard Lim; Saravanamuthu Vigneswaran; Jaya Kandasamy. Use of duckweed (Lemna disperma) to assess the phytotoxicity of the products of Fenton oxidation of metsulfuron methyl. Ecotoxicology and Environmental Safety 2012, 83, 89 -95.

AMA Style

Javeed M. Abdul, Anne Colville, Richard Lim, Saravanamuthu Vigneswaran, Jaya Kandasamy. Use of duckweed (Lemna disperma) to assess the phytotoxicity of the products of Fenton oxidation of metsulfuron methyl. Ecotoxicology and Environmental Safety. 2012; 83 ():89-95.

Chicago/Turabian Style

Javeed M. Abdul; Anne Colville; Richard Lim; Saravanamuthu Vigneswaran; Jaya Kandasamy. 2012. "Use of duckweed (Lemna disperma) to assess the phytotoxicity of the products of Fenton oxidation of metsulfuron methyl." Ecotoxicology and Environmental Safety 83, no. : 89-95.

Journal article
Published: 30 September 2008 in Ecotoxicology and Environmental Safety
Reads 0
Downloads 0

The widespread use of chlorpyrifos for pest control in urban and rural environments poses a risk of contamination to aquatic environments via runoff, spray drift or spillage. The aim of this study was to assess the fate of chlorpyrifos and its toxicity to common freshwater invertebrates in the laboratory and in stream mesocosms. Chlorpyrifos was rapidly lost from the test systems but the rates of loss varied considerably, such that losses in the mesocosms could not be reliably predicted from the static laboratory studies. This was likely due to the mass transport of chlorpyrifos from the mesocosm via stream flow. Chlorpyrifos was acutely toxic to all invertebrates tested with the cladoceran species (laboratory 48 h LC50 values 0.07–0.10 μg L−1) being most sensitive. Despite the differences in the dynamics of chlorpyrifos in the laboratory and mesocosm systems, the sensitivities of the mayfly Atalophlebia australis and the cladoceran Simocephalus vetulus were similar in the 2 systems.

ACS Style

F. Pablo; F.R. Krassoi; P.R.F. Jones; A.E. Colville; G.C. Hose; R.P. Lim. Comparison of the fate and toxicity of chlorpyrifos—Laboratory versus a coastal mesocosm system. Ecotoxicology and Environmental Safety 2008, 71, 219 -229.

AMA Style

F. Pablo, F.R. Krassoi, P.R.F. Jones, A.E. Colville, G.C. Hose, R.P. Lim. Comparison of the fate and toxicity of chlorpyrifos—Laboratory versus a coastal mesocosm system. Ecotoxicology and Environmental Safety. 2008; 71 (1):219-229.

Chicago/Turabian Style

F. Pablo; F.R. Krassoi; P.R.F. Jones; A.E. Colville; G.C. Hose; R.P. Lim. 2008. "Comparison of the fate and toxicity of chlorpyrifos—Laboratory versus a coastal mesocosm system." Ecotoxicology and Environmental Safety 71, no. 1: 219-229.

Journal article
Published: 04 December 2007 in Ecotoxicology
Reads 0
Downloads 0

This study measured the effects of a single pulse of chlorpyrifos at nominal concentrations of 1 and 10 microg/l on the macroinvertebrate community structure of a coastal stream mesocosm system. Analysis of data using Principal Response Curves (PRC) and Monte Carlo tests showed significant changes in the treated stream mesocosms relative to that of the controls. These changes in the macroinvertebrate assemblages occurred within 6 h, and persisted for at least 124 days after dosing. Significant community-level effects were detected at the lowest concentration on days 2 and 16 post-dosing, giving a no-observed effect concentration (NOEC(community)) of 1.2 microg/l (measured). The mayflies Atalophlebia sp. and Koorrnonga sp., Chironomidae and Acarina were all sensitive to chlorpyrifos and decreased in abundance in treated mesocosms after dosing. The fauna of these coastal stream mesocosms showed similar sensitivity to chlorpyrifos with that of other reported studies, but there was no evidence of recovery after 124 days.

ACS Style

Anne Colville; Peter Jones; Fleur Pablo; Frederick Krassoi; Grant Hose; Richard Lim. Effects of chlorpyrifos on macroinvertebrate communities in coastal stream mesocosms. Ecotoxicology 2007, 17, 173 -180.

AMA Style

Anne Colville, Peter Jones, Fleur Pablo, Frederick Krassoi, Grant Hose, Richard Lim. Effects of chlorpyrifos on macroinvertebrate communities in coastal stream mesocosms. Ecotoxicology. 2007; 17 (3):173-180.

Chicago/Turabian Style

Anne Colville; Peter Jones; Fleur Pablo; Frederick Krassoi; Grant Hose; Richard Lim. 2007. "Effects of chlorpyrifos on macroinvertebrate communities in coastal stream mesocosms." Ecotoxicology 17, no. 3: 173-180.