Purpose: We sought to verify if alterations in prefrontal cortex (PFC) activation and psychological responses would play along with impairments in pacing and performance of mentally fatigued cyclists. Materials and Methods: Eight recreational cyclists performed two preliminary sessions to familiarize them with the rapid visual information processing (RVP) test, psychological scales and 20 km cycling time trial (TT_20km) (session 1), as well as to perform a VO_2MAX test (session 2). Thereafter, they performed a TT_20km either after a RVP test (30 min) or a time-matched rest control session (session 3 and 4 in counterbalanced order). Performance and psychological responses were obtained throughout the TT_20km while PFC electroencephalography (EEG) was obtained at 10 and 20 km of the TT_20km and throughout the RVP test. Increases in EEG theta band power indicated a mental fatigue condition. Repeated-measures mixed models design and post-hoc effect size (ES) were used in comparisons. Results: Cyclists completed the trial ~2.7% slower in mental fatigue (34.3 ± 1.3 min) than in control (33.4 ± 1.1 min, p = 0.02, very large ES), with a lower W_MEAN (224.5 ± 17.9 W vs. 240.2 ± 20.9 W, respectively; p = 0.03; extremely large ES). There was a higher EEG theta band power during RVP test (p = 0.03; extremely large ES), which remained during the TT_20km (p = 0.01; extremely large ES). RPE increased steeper in mental fatigue than in control, together with isolated reductions in motivation at 2th km (p = 0.04; extremely large ES), felt arousal at the 2nd and 4th km (p = 0.01; extremely large ES), and associative thoughts to exercise at the 6th and 16th km (p = 0.02; extremely large ES) of the TT_20km. Conclusions: Mentally fatigued recreational cyclists showed impaired performance, altered PFC activation and faster increase in RPE during a TT_20km.

Background: Ergogenic effects of caffeine (CAF) ingestion have been observed in different cycling exercise modes, and have been associated with alterations in ratings of perceived exertion (RPE). However, there has been little investigation of maximal oxygen uptake (VO_2MAX) test outcomes. Aim: This study aimed to verify whether CAF may reduce RPE, thereby improving maximal incremental test (MIT) outcomes such as VO_2MAX, time to exhaustion and peak power output (W_PEAK). Methods: Nine healthy individuals performed three MITs (25 W/min until exhaustion) in a random, counterbalanced fashion after ingestion of CAF, placebo perceived as caffeine (PLA), and no supplementation (baseline control). VO₂ was measured throughout the test, while RPE was rated according to overall and leg effort sensations. The power output corresponding to submaximal (RPE = 14 according to the 6–20 Borg scale) and maximal RPE was recorded for both overall (O-RPE₁₄ and O-RPE_MAX) and leg RPE (L-RPE₁₄ and L-RPE_MAX). Results: VO_2MAX did not change significantly between MITs; however, CAF and PLA increased time to exhaustion (↑ ∼18.7% and ∼17.1%, respectively; p < .05) and W_PEAK (↑ ∼13.0% and ∼11.8%, respectively; p < .05) when compared with control. When compared with control, CAF ingestion reduced submaximal and maximal overall and leg RPEs, the effect being greater in maximal (likely beneficial in O-RPE_MAX and L-RPE_MAX) than submaximal RPE (possibly beneficial in O-RPE₁₄ and L-RPE₁₄). Similar results were found when participants ingested PLA. Conclusions: Compared with control, CAF and PLA improved MIT performance outcomes such as time to exhaustion and W_PEAK, without altering VO_2MAX values. CAF effects were attributed to placebo.

Pain arising from exercise potentiates fatigue and impairs the performance of endurance exercise. We assessed neurophysiological and perceptual responses to endurance exercise performed under experimentally induced muscle pain by a model that separates muscle pain from muscle fatigue. After a series of pilot studies investigating different hypertonic saline volumes, 17 healthy males performed a preliminary VO_2PEAK test before performing a familiarization of the cycling time‐to‐exhaustion exercise (80% of the peak power output in the VO_2PEAK test). Participants, performed a baseline exercise session before the sessions with hypertonic and isotonic saline injections in the vastus lateralis of both legs, in a crossover and counterbalanced design. Neurophysiological and perceptual responses such as electroencephalography (EEG) in frontal, prefrontal, parietal, and motor cortex, electromyography (EMG) of the vastus lateralis and biceps femoris muscles, ratings of perceived exertion (RPE), pain sensation, and affective valence were measured at rest and during exercise. The hypertonic injection reduced the resting EEG alpha‐beta ratio in the frontal and prefrontal cortex. When compared to exercise performed after the isotonic injection (430.5 ± 152.6 s), hypertonic injection shortened the time‐to‐exhaustion (357.5 ± 173.0 s), reduced the EMG of the assessed muscles, and increased the muscle co‐contraction during exercise. The hypertonic injection also reduced the EEG alpha‐beta ratio in the prefrontal and parietal cortex, increased RPE and pain sensation, and reduced affective valence during exercise. This proof‐of‐concept study showed that hypertonic injection‐induced muscle pain reduced endurance performance, promoting centrally mediated alterations in motor command and cortical activation, as well as an interplay of perceptual responses.

The central and peripheral effects of caffeine remain debatable. We verified whether increases in endurance performance after caffeine ingestion occurred together with changes in primary motor cortex (MC) and prefrontal cortex (PFC) activation, neuromuscular efficiency (NME), and electroencephalography–electromyography coherence (EEG–EMG coherence). Twelve participants performed a time-to-task failure isometric contraction at 70% of the maximal voluntary contraction after ingesting 5 mg/kg of caffeine (CAF) or placebo (PLA), in a crossover and counterbalanced design. MC (Cz) and PFC (Fp1) EEG alpha wave and vastus lateralis (VL) muscle EMG were recorded throughout the exercise. EEG–EMG coherence was calculated through the magnitude squared coherence analysis in MC EEG gamma-wave (CI > 0.0058). Moreover, NME was obtained as the force–VL EMG ratio. When compared to PLA, CAF improved the time to task failure (p = 0.003, d = 0.75), but reduced activation in MC and PFC throughout the exercise (p = 0.027, d = 1.01 and p = 0.045, d = 0.95, respectively). Neither NME (p = 0.802, d = 0.34) nor EEG–EMG coherence (p = 0.628, d = 0.21) was different between CAF and PLA. The results suggest that CAF improved muscular performance through a modified central nervous system (CNS) response rather than through alterations in peripheral muscle or central–peripheral coupling.