Industrial effluents/wastewater are the main sources of hexavalent chromium (Cr (VI)) pollutants in the environment. Cr (VI) pollution has become one of the world’s most serious environmental concerns due to its long persistence in the environment and highly deadly nature in living organisms. To its widespread use in industries Cr (VI) is highly toxic and one of the most common environmental contaminants. Cr (VI) is frequently non-biodegradable in nature, which means it stays in the environment for a long time, pollutes the soil and water, and poses substantial health risks to humans and wildlife. In living things, the hexavalent form of Cr is carcinogenic, genotoxic, and mutagenic. Physico-chemical techniques currently used for Cr (VI) removal are not environmentally friendly and use a large number of chemicals. Microbes have many natural or acquired mechanisms to combat chromium toxicity, such as biosorption, reduction, subsequent efflux, or bioaccumulation. This review focuses on microbial responses to chromium toxicity and the potential for their use in environmental remediation. Moreover, the research problem and prospects for the future are discussed in order to fill these gaps and overcome the problem associated with bacterial bioremediation’s real-time applicability. Graphical abstract

Scaffolds manufactured from biological materials promise better clinical functionality, providing that characteristic features are preserved. Collagen, a prominent biopolymer, is used extensively for tissue engineering applications, because its signature biological and physico-chemical properties are retained in in vitro preparations. We show here for the first time that the very properties that have established collagen as the leading natural biomaterial are lost when it is electro-spun into nano-fibres out of fluoroalcohols such as 1,1,1,3,3,3-hexafluoro-2-propanol or 2,2,2-trifluoroethanol. We further identify the use of fluoroalcohols as the major culprit in the process. The resultant nano-scaffolds lack the unique ultra-structural axial periodicity that confirms quarter-staggered supramolecular assemblies and the capacity to generate second harmonic signals, representing the typical crystalline triple-helical structure. They were also characterised by low denaturation temperatures, similar to those obtained from gelatin preparations (p>0.05). Likewise, circular dichroism spectra revealed extensive denaturation of the electro-spun collagen. Using pepsin digestion in combination with quantitative SDS-PAGE, we corroborate great losses of up to 99% of triple-helical collagen. In conclusion, electro-spinning of collagen out of fluoroalcohols effectively denatures this biopolymer, and thus appears to defeat its purpose, namely to create biomimetic scaffolds emulating the collagen structure and function of the extracellular matrix.

Polymyxins remain the last line treatment for multidrug-resistant (MDR) infections. As polymyxins resistance emerges, there is an urgent need to develop effective antimicrobial agents capable of mitigating MDR. Here, we report biodegradable guanidinium-functionalized polycarbonates with a distinctive mechanism that does not induce drug resistance. Unlike conventional antibiotics, repeated use of the polymers does not lead to drug resistance. Transcriptomic analysis of bacteria further supports development of resistance to antibiotics but not to the macromolecules after 30 treatments. Importantly, high in vivo treatment efficacy of the macromolecules is achieved in MDR A. baumannii-, E. coli-, K. pneumoniae-, methicillin-resistant S. aureus-, cecal ligation and puncture-induced polymicrobial peritonitis, and P. aeruginosa lung infection mouse models while remaining non-toxic (e.g., therapeutic index—ED₅₀/LD₅₀: 1473 for A. baumannii infection). These biodegradable synthetic macromolecules have been demonstrated to have broad spectrum in vivo antimicrobial activity, and have excellent potential as systemic antimicrobials against MDR infections.