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Two cultured cell lines (GTH4 and GTH4S) of a Nicotiana interspecific F1 hybrid (N. gossei × N. tabacum) were comparatively analyzed to find genetic factors related to hybrid inviability. Both cell lines proliferated at 37 °C, but after shifting to 26 °C, GTH4 started to die similar to the F1 hybrid seedlings, whereas GTH4S survived. As cell death requires de novo expression of genes and proteins, we compared expressed protein profiles between the two cell lines, and found that NgSGT1, a cochaperone of the chaperone complex (HSP90-SGT1-RAR1), was expressed in GTH4 but not in GTH4S. Agrobacterium-mediated transient expression of NgSGT1, but not NtSGT1, induced cell death in leaves of N. tabacum, suggesting its possible role in hybrid inviability. Cell death in N. tabacum was also induced by transient expression of NgRAR1, but not NtRAR1. In contrast, transient expression of any parental combinations of three components revealed that NgRAR1 promoted cell death, whereas NtRAR1 suppressed it in N. tabacum. A specific inhibitor of HSP90, geldanamycin, inhibited the progression of hypersensitive response-like cell death in GTH4 and leaf tissue after agroinfiltration. The present study suggested that components of the chaperone complex are involved in the inviability of Nicotiana interspecific hybrid.
Yushi Katsuyama; Mizuho Doi; Sachi Shioya; Sanae Hane; Momoko Yoshioka; Shuichi Date; Chika Miyahara; Tomomichi Ogawa; Ryo Takada; Hanako Okumura; Rie Ikusawa; Sakihito Kitajima; Kenji Oda; Kenji Sato; Yoshikazu Tanaka; Takahiro Tezuka; Masanobu Mino. The role of chaperone complex HSP90-SGT1-RAR1 as the associated machinery for hybrid inviability between Nicotiana gossei Domin and N. tabacum L. Gene 2021, 776, 145443 .
AMA StyleYushi Katsuyama, Mizuho Doi, Sachi Shioya, Sanae Hane, Momoko Yoshioka, Shuichi Date, Chika Miyahara, Tomomichi Ogawa, Ryo Takada, Hanako Okumura, Rie Ikusawa, Sakihito Kitajima, Kenji Oda, Kenji Sato, Yoshikazu Tanaka, Takahiro Tezuka, Masanobu Mino. The role of chaperone complex HSP90-SGT1-RAR1 as the associated machinery for hybrid inviability between Nicotiana gossei Domin and N. tabacum L. Gene. 2021; 776 ():145443.
Chicago/Turabian StyleYushi Katsuyama; Mizuho Doi; Sachi Shioya; Sanae Hane; Momoko Yoshioka; Shuichi Date; Chika Miyahara; Tomomichi Ogawa; Ryo Takada; Hanako Okumura; Rie Ikusawa; Sakihito Kitajima; Kenji Oda; Kenji Sato; Yoshikazu Tanaka; Takahiro Tezuka; Masanobu Mino. 2021. "The role of chaperone complex HSP90-SGT1-RAR1 as the associated machinery for hybrid inviability between Nicotiana gossei Domin and N. tabacum L." Gene 776, no. : 145443.
The genus Capsicum is comprised of 5 domesticated and more than 30 wild species. The region of nuclear ribosomal DNA internal transcribed spacers (rDNA-ITS) has widely been used for species identification, but has rarely been used in Capsicum. In this study, the evaluation of genetic diversity and a phylogenetic analysis were conducted using rDNA-ITS of 28 Capsicum accessions, including five domesticated and two wild species. We surveyed six conventional keys of domesticated species and another five traits in Capsicum accessions. Specific morphological characteristics were found in C. annuum, C. baccatum, and C.pubescens. Three subclones of each accession were sequenced, and rDNA-ITS polymorphisms were detected in all accessions excluding C. annuum, suggesting that incomplete concerted evolution occurred in rDNA-ITS of Capsicum. The genetic diversity was evaluated using nucleotide polymorphism and diversity. C. annuum had the lowest genetic diversity of all species in this study. The phylogenetic tree formed a species-specific clade for C. annuum, C. baccatum, and C. pubescens. The C. chinense clade existed in the C. frutescens clade, implying that it was a cultivated variant of C. frutescens. C. chacoense likely belonged to the C. baccatum complex according to its morphologic and genetic features. This study indicated that the rDNA-ITS region can be used for simple identification of domesticated Capsicum species.
Kumpei Shiragaki; Shuji Yokoi; Takahiro Tezuka. Phylogenetic Analysis and Molecular Diversity of Capsicum Based on rDNA-ITS Region. Horticulturae 2020, 6, 87 .
AMA StyleKumpei Shiragaki, Shuji Yokoi, Takahiro Tezuka. Phylogenetic Analysis and Molecular Diversity of Capsicum Based on rDNA-ITS Region. Horticulturae. 2020; 6 (4):87.
Chicago/Turabian StyleKumpei Shiragaki; Shuji Yokoi; Takahiro Tezuka. 2020. "Phylogenetic Analysis and Molecular Diversity of Capsicum Based on rDNA-ITS Region." Horticulturae 6, no. 4: 87.
Hybrid lethality observed in hybrid seedlings between Nicotiana suaveolens and N. tabacum is characterized by browning, initially of the hypocotyls and eventually of entire seedlings. We investigated the mechanism underlying this browning of tissues. A phenylalanine ammonia-lyase (PAL) gene codes an enzyme involved in a pathway producing phenolic compounds related to the browning of plant tissues. The expression of PAL rapidly increased with the induction of hybrid lethality. Phenolic compounds were observed to be accumulated in whole parts of hybrid seedlings. Treatment of hybrid seedlings with L-2-aminooxy-3-phenylpropionic acid (AOPP), an inhibitor for PAL, suppressed browning and decreased the phenolic content of hybrid seedlings. Although programmed cell death (PCD) was involved in hybrid lethality, AOPP treatment also suppressed cell death and enhanced the growth of hybrid seedlings. These results indicated that PAL is involved in hybrid lethality, and phenolic compounds could be the cause of hybrid lethality-associated tissue browning.
Kumpei Shiragaki; Rie Nakamura; Shigeki Nomura; Hai He; Tetsuya Yamada; Wataru Marubashi; Masayuki Oda; Takahiro Tezuka. Phenylalanine ammonia-lyase and phenolic compounds are related to hybrid lethality in the cross Nicotiana suaveolens×N. tabacum. Plant Biotechnology 2020, 37, 327 -333.
AMA StyleKumpei Shiragaki, Rie Nakamura, Shigeki Nomura, Hai He, Tetsuya Yamada, Wataru Marubashi, Masayuki Oda, Takahiro Tezuka. Phenylalanine ammonia-lyase and phenolic compounds are related to hybrid lethality in the cross Nicotiana suaveolens×N. tabacum. Plant Biotechnology. 2020; 37 (3):327-333.
Chicago/Turabian StyleKumpei Shiragaki; Rie Nakamura; Shigeki Nomura; Hai He; Tetsuya Yamada; Wataru Marubashi; Masayuki Oda; Takahiro Tezuka. 2020. "Phenylalanine ammonia-lyase and phenolic compounds are related to hybrid lethality in the cross Nicotiana suaveolens×N. tabacum." Plant Biotechnology 37, no. 3: 327-333.
Seed abortion and ovary abscission, two types of postzygotic reproductive barriers, are often observed in interspecific and/or interploidy crosses in plants. However, the mechanisms underlying these reproductive barriers remain unclear. Here, we show that the distinct types of seed developmental abnormalities (type I and type II seed abortion) occur in a phased manner as maternal to paternal genome dosage increases and that type II seed abortion is followed by ovary abscission. We revealed that these two types of seed developmental abnormalities are observed during seed development in the interploidy‐interspecific crosses of Nicotiana suaveolens and N. tabacum. Moreover, in the cross showing type II seed abortion, several events, such as changes in abscission‐related gene expression and lignin deposition, occurred in the ovary abscission zone, eventually leading to ovary abscission. Notably, successive increases in maternal ploidy using ploidy manipulated lines resulted in successive type I and type II seed abortions, and the latter was accompanied by ovary abscission. Conversely, both types of seed abortion and ovary abscission could be overcome with a ploidy manipulation technique that balances parental ploidy levels. We thus concluded that a high maternal genome excess cross may cause severe seed developmental defects and ovary abscission. Based on our findings, we propose a model explaining the abortion phenomena, where an interaction between the promotive and inhibitive effects of the parental genomes determines the developmental destiny of seeds. Significance statement We demonstrate that a stepwise increase in maternal ploidy results in a stepwise increase in seed abortion severity, leading to ovary abscission in plants. We propose a model explaining the abortion phenomena, where an interaction between the promotive and inhibitive effects of the parental genomes determines the developmental destiny of seeds.
Hai He; Shuji Yokoi; Takahiro Tezuka. A high maternal genome excess causes severe seed abortion leading to ovary abscission in Nicotiana interploidy‐interspecific crosses. Plant Direct 2020, 4, e00257 .
AMA StyleHai He, Shuji Yokoi, Takahiro Tezuka. A high maternal genome excess causes severe seed abortion leading to ovary abscission in Nicotiana interploidy‐interspecific crosses. Plant Direct. 2020; 4 (8):e00257.
Chicago/Turabian StyleHai He; Shuji Yokoi; Takahiro Tezuka. 2020. "A high maternal genome excess causes severe seed abortion leading to ovary abscission in Nicotiana interploidy‐interspecific crosses." Plant Direct 4, no. 8: e00257.
Hybrid weakness in Capsicum is characterized by the termination of leaf differentiation after the development of several leaves. F1 plants in some crosses between Capsicum annuum and Capsicum chinense show weakness; this phenomenon has not been investigated in detail since first reported. In the present study, we characterized morphologically and physiologically hybrid weakness in Capsicum. F1 plants did not show weaker growth than their parents 20 days after germination (DAG), but at 40 DAG, the hybrid weakness phenotype was evidenced by almost complete arrest of new leaf formation, delayed increase in plant height, and reduced upper internode length. The shoot apical meristem (SAM) of F1 plants exhibited delayed development and an abnormal structure characterized by a flat shape and the presence of fuzzy cell layers on the surface. These abnormal SAMs of F1 plants may lead to dwarfism. Dead cells and accumulation of H2O2 were visually detected in leaves of F1 plants, and cell death was considered to be programmed, as it was accompanied by internucleosomal fragmentation of DNA. The expression of immunity marker genes PR1 and PR2 was upregulated in leaves of F1 plants. These results suggest that a hypersensitive response-like reaction is involved in Capsicum hybrid weakness.
Kumpei Shiragaki; Shuji Yokoi; Takahiro Tezuka. A hypersensitive response-like reaction is involved in hybrid weakness in F1 plants of the cross Capsicum annuum × Capsicum chinense. Breeding Science 2020, 70, 19137 -437.
AMA StyleKumpei Shiragaki, Shuji Yokoi, Takahiro Tezuka. A hypersensitive response-like reaction is involved in hybrid weakness in F1 plants of the cross Capsicum annuum × Capsicum chinense. Breeding Science. 2020; 70 (4):19137-437.
Chicago/Turabian StyleKumpei Shiragaki; Shuji Yokoi; Takahiro Tezuka. 2020. "A hypersensitive response-like reaction is involved in hybrid weakness in F1 plants of the cross Capsicum annuum × Capsicum chinense." Breeding Science 70, no. 4: 19137-437.
Hybrid weakness is a type of reproductive isolation in which F1 hybrids of normal parents exhibit weaker growth characteristics than their parents. F1 hybrid of the Oryza sativa Indian cultivars ‘P.T.B.7′ and ‘A.D.T.14′ exhibits hybrid weakness that is associated with the HWA1 and HWA2 loci. Accordingly, the aim of the present study was to analyze the hybrid weakness phenotype of the ‘P.T.B.7′ × ‘A.D.T.14′ hybrids. The height and tiller number of the F1 hybrid were lower than those of either parent, and F1 hybrid also exhibited leaf yellowing that was not observed in either parent. In addition, the present study demonstrates that SPAD values, an index correlated with chlorophyll content, are effective for evaluating the progression of hybrid weakness that is associated with the HWA1 and HWA2 loci because it accurately reflects degree of leaf yellowing. Both cell death and H2O2, a reactive oxygen species, were detected in the yellowing leaves of the F1 hybrid. Furthermore, disease resistance-related genes were upregulated in the yellowing leaves of the F1 hybrids, whereas photosynthesis-related genes tended to be downregulated. These results suggest that the hybrid weakness associated with the HWA1 and HWA2 loci involves hypersensitive response-like mechanisms.
Kumpei Shiragaki; Takahiro Iizuka; Katsuyuki Ichitani; Tsutomu Kuboyama; Toshinobu Morikawa; Masayuki Oda; Takahiro Tezuka. HWA1- and HWA2-Mediated Hybrid Weakness in Rice Involves Cell Death, Reactive Oxygen Species Accumulation, and Disease Resistance-Related Gene Upregulation. Plants 2019, 8, 450 .
AMA StyleKumpei Shiragaki, Takahiro Iizuka, Katsuyuki Ichitani, Tsutomu Kuboyama, Toshinobu Morikawa, Masayuki Oda, Takahiro Tezuka. HWA1- and HWA2-Mediated Hybrid Weakness in Rice Involves Cell Death, Reactive Oxygen Species Accumulation, and Disease Resistance-Related Gene Upregulation. Plants. 2019; 8 (11):450.
Chicago/Turabian StyleKumpei Shiragaki; Takahiro Iizuka; Katsuyuki Ichitani; Tsutomu Kuboyama; Toshinobu Morikawa; Masayuki Oda; Takahiro Tezuka. 2019. "HWA1- and HWA2-Mediated Hybrid Weakness in Rice Involves Cell Death, Reactive Oxygen Species Accumulation, and Disease Resistance-Related Gene Upregulation." Plants 8, no. 11: 450.
Morphological and genetic markers indicate that in sorghum, the juvenile-to-adult phase transition occurs during the fourth and fifth leaf stages. This timing differs from those reported for other plants. The juvenile-to-adult (JA) phase transition is an important event for optimizing vegetative growth and reproductive success in plants. Among the Poaceae crops, which are a vital food source for humans, studies of the JA phase transition have been restricted to rice and maize. We studied the morphological and genetic changes that occur during the early development of sorghum and found that dramatic changes occur in shoot architecture during the early vegetative stages. Changes were observed in leaf size, leaf shape, numbers of trichomes, and size of the shoot apical meristem. In particular, the length/width ratios of the leaf blades in the fifth and upper leaves were completely different from those of the second to fourth leaves. The fifth and upper leaves have trichomes on their adaxial sides, which were absent on the lower leaves. We also analyzed expression of two microRNAs that are known to be molecular markers of the JA phase transition and found that expression of miR156 was highest in the second to fourth leaves and then was gradually down-regulated, whereas miR172 expression followed the opposite pattern. These results suggest that in sorghum, the second and third leaves represent the juvenile phase, the fourth and fifth leaves are in the transition stage, and the sixth and upper leaves are in the adult phase. Thus, the JA phase transition occurs during the fourth and fifth leaf stages. These findings are expected to be useful for understanding the early development of sorghum.
Shumpei Hashimoto; Takahiro Tezuka; Shuji Yokoi. Morphological changes during juvenile-to-adult phase transition in sorghum. Planta 2019, 250, 1557 -1566.
AMA StyleShumpei Hashimoto, Takahiro Tezuka, Shuji Yokoi. Morphological changes during juvenile-to-adult phase transition in sorghum. Planta. 2019; 250 (5):1557-1566.
Chicago/Turabian StyleShumpei Hashimoto; Takahiro Tezuka; Shuji Yokoi. 2019. "Morphological changes during juvenile-to-adult phase transition in sorghum." Planta 250, no. 5: 1557-1566.
Takahiro Tezuka; Katsuyuki Ichitani; Yuichi Matsumoto; Hai He; Tetsu Kinoshita; Shigeo Takumi; Tsutomu Kuboyama. Toward the elucidation of reproductive isolation mechanisms in plants: diversity and universality. Breeding Research 2019, 21, 75 -80.
AMA StyleTakahiro Tezuka, Katsuyuki Ichitani, Yuichi Matsumoto, Hai He, Tetsu Kinoshita, Shigeo Takumi, Tsutomu Kuboyama. Toward the elucidation of reproductive isolation mechanisms in plants: diversity and universality. Breeding Research. 2019; 21 (1):75-80.
Chicago/Turabian StyleTakahiro Tezuka; Katsuyuki Ichitani; Yuichi Matsumoto; Hai He; Tetsu Kinoshita; Shigeo Takumi; Tsutomu Kuboyama. 2019. "Toward the elucidation of reproductive isolation mechanisms in plants: diversity and universality." Breeding Research 21, no. 1: 75-80.
Reproductive isolation, including prezygotic and postzygotic barriers, is a mechanism that separates species. Many species in the Nicotiana section Suaveolentes exhibit reproductive isolation in crosses with Nicotiana tabacum. In this study, we investigated whether the chromosome numbers and ploidy levels of eight Nicotiana suaveolens accessions are related to the reproductive isolation after crosses with N. tabacum by flow cytometry and chromosome analyses. Additionally, the internal transcribed spacer (ITS) regions of the eight N. suaveolens accessions were sequenced and compared with the previously reported sequences of 22 Suaveolentes species to elucidate the phylogenetic relationships in the section Suaveolentes. We revealed that four N. suaveolens accessions comprised 64 chromosomes, while the other four accessions carried 32 chromosomes. Depending on the ploidy levels of N. suaveolens, several types of reproductive isolation were observed after crosses with N. tabacum, including decreases in the number of capsules and the germination rates of hybrid seeds, as well as hybrid lethality and abscission of enlarged ovaries at 12-17 days after pollination. A phylogenetic analysis involving ITS sequences divided the eight N. suaveolens accessions into three distinct clades. Based on the results, we confirmed that N. suaveolens accessions vary regarding ploidy levels and reproductive isolation mechanisms in crosses with N. tabacum. These accessions will be very useful for revealing and characterizing the reproductive isolation mechanisms in interspecific crosses and their relationships with ploidy levels.
Hai He; Takahiro Iizuka; Maho Maekawa; Kumi Sadahisa; Toshinobu Morikawa; Masanori Yanase; Shuji Yokoi; Masayuki Oda; Takahiro Tezuka. Nicotiana suaveolens accessions with different ploidy levels exhibit different reproductive isolation mechanisms in interspecific crosses with Nicotiana tabacum. Journal of Plant Research 2019, 132, 461 -471.
AMA StyleHai He, Takahiro Iizuka, Maho Maekawa, Kumi Sadahisa, Toshinobu Morikawa, Masanori Yanase, Shuji Yokoi, Masayuki Oda, Takahiro Tezuka. Nicotiana suaveolens accessions with different ploidy levels exhibit different reproductive isolation mechanisms in interspecific crosses with Nicotiana tabacum. Journal of Plant Research. 2019; 132 (4):461-471.
Chicago/Turabian StyleHai He; Takahiro Iizuka; Maho Maekawa; Kumi Sadahisa; Toshinobu Morikawa; Masanori Yanase; Shuji Yokoi; Masayuki Oda; Takahiro Tezuka. 2019. "Nicotiana suaveolens accessions with different ploidy levels exhibit different reproductive isolation mechanisms in interspecific crosses with Nicotiana tabacum." Journal of Plant Research 132, no. 4: 461-471.
Hideyuki Tanaka; Koji Murai; Takao Nakanishi; Takahiro Tezuka; Shuji Shiozaki; Masayuki Oda. Storage of plug seedlings of tomato under limited fertilisation, and growth, flowering and yield after planting. The Journal of Horticultural Science and Biotechnology 2018, 93, 652 -658.
AMA StyleHideyuki Tanaka, Koji Murai, Takao Nakanishi, Takahiro Tezuka, Shuji Shiozaki, Masayuki Oda. Storage of plug seedlings of tomato under limited fertilisation, and growth, flowering and yield after planting. The Journal of Horticultural Science and Biotechnology. 2018; 93 (6):652-658.
Chicago/Turabian StyleHideyuki Tanaka; Koji Murai; Takao Nakanishi; Takahiro Tezuka; Shuji Shiozaki; Masayuki Oda. 2018. "Storage of plug seedlings of tomato under limited fertilisation, and growth, flowering and yield after planting." The Journal of Horticultural Science and Biotechnology 93, no. 6: 652-658.
Masahumi Johkan; Masaki Ono; Hideyuki Tanaka; Hajime Furukawa; Takahiro Tezuka; Masayuki Oda. Morphological Variation, Growth, and Yield of Tomato Plants Vegetatively Propagated by the Complete Decapitation Method. International Journal of Vegetable Science 2014, 22, 58 -65.
AMA StyleMasahumi Johkan, Masaki Ono, Hideyuki Tanaka, Hajime Furukawa, Takahiro Tezuka, Masayuki Oda. Morphological Variation, Growth, and Yield of Tomato Plants Vegetatively Propagated by the Complete Decapitation Method. International Journal of Vegetable Science. 2014; 22 (1):58-65.
Chicago/Turabian StyleMasahumi Johkan; Masaki Ono; Hideyuki Tanaka; Hajime Furukawa; Takahiro Tezuka; Masayuki Oda. 2014. "Morphological Variation, Growth, and Yield of Tomato Plants Vegetatively Propagated by the Complete Decapitation Method." International Journal of Vegetable Science 22, no. 1: 58-65.
Takanobu Higashi; Akiko Kamitamari; Nobuya Okamura; Kazuya Ukai; Kenichi Okamura; Takahiro Tezuka; Hirokazu Fukuda. Characterization of Circadian Rhythms Through a Bioluminescence Reporter Assay in Lactuca sativa L. Environment Control in Biology 2014, 52, 21 -27.
AMA StyleTakanobu Higashi, Akiko Kamitamari, Nobuya Okamura, Kazuya Ukai, Kenichi Okamura, Takahiro Tezuka, Hirokazu Fukuda. Characterization of Circadian Rhythms Through a Bioluminescence Reporter Assay in Lactuca sativa L. Environment Control in Biology. 2014; 52 (1):21-27.
Chicago/Turabian StyleTakanobu Higashi; Akiko Kamitamari; Nobuya Okamura; Kazuya Ukai; Kenichi Okamura; Takahiro Tezuka; Hirokazu Fukuda. 2014. "Characterization of Circadian Rhythms Through a Bioluminescence Reporter Assay in Lactuca sativa L." Environment Control in Biology 52, no. 1: 21-27.
Genetic tumors are neoplastic growths that arise spontaneously in particular genotypes in plants. In the present study, we observed the occurrence of tumors in interspecific hybrids between Solanum lycopersicum L. and Solanum habrochaites S. Knapp and D. M. Spooner. The hybridity of these plants was confirmed based on morphological characteristics, flow cytometry and random amplified polymorphic DNA analysis. Hybrids formed tumors when the plants were grown in a growth chamber. These tumors were formed most frequently on leaves, but also on cotyledons, stems and petioles. When hybrid plants bearing tumors were transferred to a greenhouse, the tumors disappeared and necrotic brown spots were visible in the positions where tumors existed previously. Further analyses indicated that tumors were never formed in the greenhouse. These results suggest that environmental conditions influenced tumorigenesis in hybrids raised from S. lycopersicum × S. habrochaites crosses. In addition, when stem segments of hybrids were cultured in vitro on plant growth regulator-free medium, tumors and shoot formation were induced. Thus, hybrids between S. lycopersicum × S. habrochaites provide an excellent experimental system to study plant tumorigenesis and to understand cell division and differentiation. Key words: Environmental condition, growth abnormality, interspecific cross, tomato, tissue culture, tumor.
Tezuka Takahiro; Okinaka Hidenobu; Oda Masayuki; Takahiro Tezuka; Hidenobu Okinaka; Masayuki Oda. Tumor formation in hybrids between Solanum lycopersicum and S. habrochaites. African Journal of Biotechnology 2013, 12, 1745 -1753.
AMA StyleTezuka Takahiro, Okinaka Hidenobu, Oda Masayuki, Takahiro Tezuka, Hidenobu Okinaka, Masayuki Oda. Tumor formation in hybrids between Solanum lycopersicum and S. habrochaites. African Journal of Biotechnology. 2013; 12 (15):1745-1753.
Chicago/Turabian StyleTezuka Takahiro; Okinaka Hidenobu; Oda Masayuki; Takahiro Tezuka; Hidenobu Okinaka; Masayuki Oda. 2013. "Tumor formation in hybrids between Solanum lycopersicum and S. habrochaites." African Journal of Biotechnology 12, no. 15: 1745-1753.
Mature seeds of Ilex species usually contain immature embryos and are extremely difficult to germinate. Ilex latifolia and I. rotunda, two species that are grown as ornamentals, also produce seeds that are difficult to germinate. In the present study, we investigated some factors affecting seed germination in those species. Although seeds of I. latifolia and I. rotunda could imbibe water, they did not germinate. When embryos were cultured in vitro, germination was observed in I. latifolia but not in I. rotunda. Interestingly, a transient decrease in germination frequency occurred in I. latifolia embryos isolated from seeds collected in September or October. Among five types of I. latifolia seeds that differed in the presence of the endocarp, testa, and endosperm, germinability of isolated embryos was highest. Good germination was also observed in quarter-seeds with or without endocarp followed by half-seeds. Treatment of seeds with sodium hydroxide (NaOH) had no effect on seed germination in I. latifolia. Cold stratification at 5 °C increased the germination frequency of I. latifolia embryos. In conclusion, the data suggested a mechanical barrier by the endocarp and inhibitors contained in the endosperm, testa, and/or endocarp inhibited seed germination in I. latifolia. Although no seeds or embryos of I. rotunda germinated, cold stratification in combination with other treatments deserves further investigation. Chemical name: sodium hydroxide (NaOH).
Takahiro Tezuka; Hisa Yokoyama; Hideyuki Tanaka; Shuji Shiozaki; Masayuki Oda. Factors Affecting Seed Germination of Ilex latifolia and I. rotunda. HortScience 2013, 48, 352 -356.
AMA StyleTakahiro Tezuka, Hisa Yokoyama, Hideyuki Tanaka, Shuji Shiozaki, Masayuki Oda. Factors Affecting Seed Germination of Ilex latifolia and I. rotunda. HortScience. 2013; 48 (3):352-356.
Chicago/Turabian StyleTakahiro Tezuka; Hisa Yokoyama; Hideyuki Tanaka; Shuji Shiozaki; Masayuki Oda. 2013. "Factors Affecting Seed Germination of Ilex latifolia and I. rotunda." HortScience 48, no. 3: 352-356.
Hideyuki Tanaka; Masahumi Johkan; Kazuhiko Mitsukuri; Takahiro Tezuka; Hajime Furukawa; Masayuki Oda. Intact roots promote shoot regeneration from hypocotyl independent of exogenous plant growth regulators in eggplant in vitro. Plant Root 2013, 7, 5 -11.
AMA StyleHideyuki Tanaka, Masahumi Johkan, Kazuhiko Mitsukuri, Takahiro Tezuka, Hajime Furukawa, Masayuki Oda. Intact roots promote shoot regeneration from hypocotyl independent of exogenous plant growth regulators in eggplant in vitro. Plant Root. 2013; 7 ():5-11.
Chicago/Turabian StyleHideyuki Tanaka; Masahumi Johkan; Kazuhiko Mitsukuri; Takahiro Tezuka; Hajime Furukawa; Masayuki Oda. 2013. "Intact roots promote shoot regeneration from hypocotyl independent of exogenous plant growth regulators in eggplant in vitro." Plant Root 7, no. : 5-11.
Ardisia crenata is an evergreen shrub with attractive bright red berries. Although this species is usually propagated by seed, the seeds take a long time to germinate with conventional sowing methods. We investigated the germination capacity of seeds and embryos collected in different months and the effects of seed storage conditions, germination temperature, water permeability of the seed coat, and the endosperm on seed germination. Seeds and embryos collected in late September or later showed good germination rates. Seeds germinated more rapidly after longer periods of storage at low temperature (approximately 5°C), and those stored in dry conditions showed lower emergence frequency than those stored in wet conditions. Seeds germinated at 15–30°C, but not at 5–10°C. Removal of the seed coat enhanced water uptake and seed germination. Seeds with various proportions of the removed seed coat were sown on a medium supplemented with sucrose. The germination frequency increased as the size of the remaining endosperm decreased. However, the opposite results were obtained when seeds were sown on a medium without sucrose. We concluded that the optimal temperature of 25°C is the most critical factor for seed germination in A. crenata.
Takahiro Tezuka; Hisa Yokoyama; Hideyuki Tanaka; Shuji Shiozaki; Masayuki Oda. Seed and Embryo Germination in Ardisia crenata. Journal of Botany 2012, 2012, 1 -7.
AMA StyleTakahiro Tezuka, Hisa Yokoyama, Hideyuki Tanaka, Shuji Shiozaki, Masayuki Oda. Seed and Embryo Germination in Ardisia crenata. Journal of Botany. 2012; 2012 ():1-7.
Chicago/Turabian StyleTakahiro Tezuka; Hisa Yokoyama; Hideyuki Tanaka; Shuji Shiozaki; Masayuki Oda. 2012. "Seed and Embryo Germination in Ardisia crenata." Journal of Botany 2012, no. : 1-7.
A linkage map consisting of 24 linkage groups has been constructed using simple sequence repeat (SSR) markers in Nicotiana tabacum. However, chromosomal assignments of all linkage groups have not yet been made. The Q chromosome in N. tabacum encodes a gene or genes triggering hybrid lethality, a phenomenon that causes death of hybrids derived from some crosses. We identified a linkage group corresponding to the Q chromosome using an interspecific cross between an N. tabacum monosomic line lacking the Q chromosome and N. africana. N. ingulba yielded inviable hybrids after crossing with N. tabacum. SSR markers on the identified linkage group were used to analyze hybrid lethality in this cross. The results implied that one or more genes on the Q chromosome are responsible for hybrid lethality in this cross. Furthermore, the gene(s) responsible for hybrid lethality in the cross N. tabacum × N. africana appear to be on the region of the Q chromosome to which SSR markers PT30342 and PT30365 map. Linkage group 11 corresponded to the Q chromosome. We propose a new method to correlate linkage groups with chromosomes in N. tabacum.
Takahiro Tezuka; Chihiro Matsuo; Takahiro Iizuka; Masayuki Oda; Wataru Marubashi. Identification of Nicotiana tabacum Linkage Group Corresponding to the Q Chromosome Gene(s) Involved in Hybrid Lethality. PLOS ONE 2012, 7, e37822 .
AMA StyleTakahiro Tezuka, Chihiro Matsuo, Takahiro Iizuka, Masayuki Oda, Wataru Marubashi. Identification of Nicotiana tabacum Linkage Group Corresponding to the Q Chromosome Gene(s) Involved in Hybrid Lethality. PLOS ONE. 2012; 7 (5):e37822.
Chicago/Turabian StyleTakahiro Tezuka; Chihiro Matsuo; Takahiro Iizuka; Masayuki Oda; Wataru Marubashi. 2012. "Identification of Nicotiana tabacum Linkage Group Corresponding to the Q Chromosome Gene(s) Involved in Hybrid Lethality." PLOS ONE 7, no. 5: e37822.
Many species of Nicotiana section Suaveolentes produce inviable F1 hybrids after crossing with Nicotiana tabacum (genome constitution SSTT), a phenomenon that is often called hybrid lethality. Through crosses with monosomic lines of N. tabacum lacking a Q chromosome, we previously determined that hybrid lethality is caused by interaction between gene(s) on the Q chromosome belonging to the S subgenome of N. tabacum and gene(s) in Suaveolentes species. Here, we examined if hybrid seedlings from the cross N. occidentalis (section Suaveolentes)×N. tabacum are inviable despite a lack of the Q chromosome. Hybrid lethality in the cross of N. occidentalis×N. tabacum was characterized by shoots with fading color. This symptom differed from what has been previously observed in lethal crosses between many species in section Suaveolentes and N. tabacum. In crosses of monosomic N. tabacum plants lacking the Q chromosome with N. occidentalis, hybrid lethality was observed in hybrid seedlings either lacking or possessing the Q chromosome. N. occidentalis was then crossed with two progenitors of N. tabacum, N. sylvestris (SS) and N. tomentosiformis (TT), to reveal which subgenome of N. tabacum contains gene(s) responsible for hybrid lethality. Hybrid seedlings from the crosses N. occidentalis×N. tomentosiformis and N. occidentalis×N. sylvestris were inviable. Although the specific symptoms of hybrid lethality in the cross N. occidentalis×N. tabacum were similar to those appearing in hybrids from the cross N. occidentalis×N. tomentosiformis, genes in both the S and T subgenomes of N. tabacum appear responsible for hybrid lethality in crosses with N. occidentalis.
Takahiro Tezuka; Wataru Marubashi. Genes in S and T Subgenomes Are Responsible for Hybrid Lethality in Interspecific Hybrids between Nicotiana tabacum and Nicotiana occidentalis. PLOS ONE 2012, 7, e36204 .
AMA StyleTakahiro Tezuka, Wataru Marubashi. Genes in S and T Subgenomes Are Responsible for Hybrid Lethality in Interspecific Hybrids between Nicotiana tabacum and Nicotiana occidentalis. PLOS ONE. 2012; 7 (4):e36204.
Chicago/Turabian StyleTakahiro Tezuka; Wataru Marubashi. 2012. "Genes in S and T Subgenomes Are Responsible for Hybrid Lethality in Interspecific Hybrids between Nicotiana tabacum and Nicotiana occidentalis." PLOS ONE 7, no. 4: e36204.
Hideyuki Tanaka; Shuji Shiozaki; Satoshi Yamasaki; Kazuhiko Mitsukuri; Yukiko Shimada; Takahiro Tezuka; Masayuki Oda. Adventitious Bud Formation Promoted by Auxin Treatment in Explants from the Apical Portion of Leaves in Begonia^|^times;tuberhybrida. Environment Control in Biology 2012, 50, 319 -327.
AMA StyleHideyuki Tanaka, Shuji Shiozaki, Satoshi Yamasaki, Kazuhiko Mitsukuri, Yukiko Shimada, Takahiro Tezuka, Masayuki Oda. Adventitious Bud Formation Promoted by Auxin Treatment in Explants from the Apical Portion of Leaves in Begonia^|^times;tuberhybrida. Environment Control in Biology. 2012; 50 (4):319-327.
Chicago/Turabian StyleHideyuki Tanaka; Shuji Shiozaki; Satoshi Yamasaki; Kazuhiko Mitsukuri; Yukiko Shimada; Takahiro Tezuka; Masayuki Oda. 2012. "Adventitious Bud Formation Promoted by Auxin Treatment in Explants from the Apical Portion of Leaves in Begonia^|^times;tuberhybrida." Environment Control in Biology 50, no. 4: 319-327.
Adventitious shoots can be regenerated from the cut surface of the primary shoot and lateral branches in decapitated plants in vivo. This inherent regenerative ability of plants is useful for mass propagation. In the present study, we conducted histological observations of shoot regeneration and applied auxin and cytokinin to decapitated seedlings in four tomato cultivars. The cultivars produced different numbers of adventitious shoots after decapitation; ‘Petit’ produced the largest number of adventitious shoots (78.5 ± 10.2) and ‘Momotaro’ produced the fewest (12.1 ± 3.3). Histological observation of ‘Petit’ revealed that adventitious shoots regenerated from calli formed at the cut surface of stems. Adventitious shoot formation was inhibited by the presence of lateral branches. Shoot regeneration was prevented by application of 1-naphthaleneacetic acid to ‘Petit’. Application of 6-benzyladenine promoted shoot regeneration in ‘Momotaro’. These results suggest auxin synthesized de novo from the lateral branches inhibited shoot regeneration after decapitation and endogenous cytokinin might stimulate shoot regeneration. Chemical names: 1-naphthaleneacetic acid (NAA); 6-benzyladenine (BA)
Takahiro Tezuka; Masashi Harada; Masahumi Johkan; Satoshi Yamasaki; Hideyuki Tanaka; Masayuki Oda. Effects of Auxin and Cytokinin on In Vivo Adventitious Shoot Regeneration from Decapitated Tomato Plants. HortScience 2011, 46, 1661 -1665.
AMA StyleTakahiro Tezuka, Masashi Harada, Masahumi Johkan, Satoshi Yamasaki, Hideyuki Tanaka, Masayuki Oda. Effects of Auxin and Cytokinin on In Vivo Adventitious Shoot Regeneration from Decapitated Tomato Plants. HortScience. 2011; 46 (12):1661-1665.
Chicago/Turabian StyleTakahiro Tezuka; Masashi Harada; Masahumi Johkan; Satoshi Yamasaki; Hideyuki Tanaka; Masayuki Oda. 2011. "Effects of Auxin and Cytokinin on In Vivo Adventitious Shoot Regeneration from Decapitated Tomato Plants." HortScience 46, no. 12: 1661-1665.