The production and consumption of nuts are increasing in the world due to strong economic returns and the nutritional value of their products. With the increasing role and importance given to nuts (i.e., walnuts, hazelnut, pistachio, pecan, almond) in a balanced and healthy diet and their benefits to human health, breeding of the nuts species has also been stepped up. Most recent fruit breeding programs have focused on scion genetic improvement. However, the use of locally adapted grafted rootstocks also enhanced the productivity and quality of tree fruit crops. Grafting is an ancient horticultural practice used in nut crops to manipulate scion phenotype and productivity and overcome biotic and abiotic stresses. There are complex rootstock breeding objectives and physiological and molecular aspects of rootstock–scion interactions in nut crops. In this review, we provide an overview of these, considering the mechanisms involved in nutrient and water uptake, regulation of phytohormones, and rootstock influences on the scion molecular processes, including long-distance gene silencing and trans-grafting. Understanding the mechanisms resulting from rootstock × scion × environmental interactions will contribute to developing new rootstocks with resilience in the face of climate change, but also of the multitude of diseases and pests.

With climate change and decreased water supplies, interest in irrigation scheduling based on plant water status is increasing. Stem water potential (Ψ_SWP) thresholds for irrigation scheduling in olive have been proposed, however, a physiologically-based evaluation of their reliability is needed. A large dataset collected at variable environmental conditions, growing systems, and genotypes was used to characterize the relation between Ψ_SWP and gas exchanges for olive. Based on the effect of drought stress on the ecophysiological parameters monitored, we described three levels of stress: no stress (Ψ_SWP above about −2 MPa), where the high variability of stomatal conductance (g_s) suggests a tight stomatal control of water loss that limit Ψ_SWP drop, irrigation volumes applied to overcome this threshold had no effect on assimilation but reduced intrinsic water use efficiency (iWUE); moderate-stress (Ψ_SWP between about −2.0 and −3.5 MPa), where iWUE can be increased without damage to the photosynthetic apparatus of leaves; and high-stress (Ψ_SWP below about −3.5 MPa), where g_s dropped below 150 mmol m⁻² s⁻¹ and the intercellular CO₂ concentration increased proportionally, suggesting non-stomatal limitation to photosynthesis was operative. This study confirmed that olive Ψ_SWP should be maintained between −2 and −3.5 MPa for optimal irrigation efficiency and to avoid harmful water stress levels.

The effects of passive- and active-modified atmosphere packaging (passive- and active-MAP) were investigated on the physio-chemical and quality attributes of fresh in-hull pistachios stored at 4 ± 1 °C and 90 ± 5% R.H. Fresh pistachios were packaged under each of the following gas combinations: active-MAP1 (AMA1) (5% O₂ + 5% CO₂), AMA2 (5% O₂ + 25% CO₂), AMA3 (5% O₂ + 45% CO₂), AMA4 (2.5% O₂ + 5% CO₂), AMA5 (2.5% O₂ + 25% CO₂), and AMA6 (2.5% O₂ + 45% CO₂), all balanced with N₂, as well as passive-MAP (PMA) with ambient air (21% O₂ + 0.03% CO₂ + 78% N₂). Changes in quality parameters were evaluated after 0, 15, 30 and 45 days of storage. Results demonstrated that AMA6 and PMA had significantly lower (7.96 Log CFU g⁻¹) and higher (9.81 Log CFU g⁻¹) aerobic mesophilic bacteria counts than the other treatments. However, the AMA6 treatment decreased, kernel chlorophyll and carotenoid content, hull antioxidant capacity, and anthocyanin content. The PMA treatment produced a significant weight loss, 0.18%, relative to the other treatments. The active-MAP treatments were more effective than the passive-MAP in decreasing weight loss, microbial counts, kernel total chlorophyll (Kernel TCL), and kernel carotenoid content (Kernel CAC). The postharvest quality of fresh in-hull pistachios was maintained best by the AMA3 (5% O₂ + 45% CO₂ + 50% N₂) treatment.