Amino acid permeases (AAPs) are proteins of the integral membrane that play important roles in plant growth, development, and responses to various stresses. The molecular functions of several AAPs were characterized in Arabidopsis and rice, but there is still limited information on wheat. Here, we identified 51 AAP genes (TaAAPs) in the wheat genome, classified into six groups based on phylogenetic and protein structures. The chromosome location and gene duplication analysis showed that gene duplication events played a crucial role in the expansion of the TaAAPs gene family. Collinearity relationship analysis revealed several orthologous AAPs between wheat and other species. Moreover, cis-element analysis of promoter regions and transcriptome data suggested that the TaAAPs can respond to salt stress. A TaAAP1 gene was selected and transformed in wheat. Overexpressing TaAAP1 enhanced salt tolerance by increasing the expression of ethylene synthesis genes (TaACS6/TaACS7/TaACS8) and accumulating more ethylene. The present study provides an overview of the AAP family in the wheat genome as well as information on systematics, phylogenetics, and gene duplication, and shows that overexpressing TaAAP1 enhances salt tolerance by regulating ethylene production. These results serve as a theoretical foundation for further functional studies on TaAAPs in the future.

Plant reproductive development is dependent on successful pollen-pistil interactions. The high degree of specificity in pollen-pistil interactions and the precision of directional pollen tube growth suggest that signals are continually being exchanged between pollen tubes and cells of the pistil that line their path. However, with few exceptions, little is known about the genes that control these interactions. In this investigation, we performed genome-wide transcriptional profiling of pistil tissues of Gametophytic factor1-Strong (Ga1-S) genotype maize after pollination 5 hours(5HAP)with Ga1-S pollen and ga1 pollen respectively , in an attempt to isolate genes involved in pollen tube elongation in maize pistils, using the Solexa deep sequencing technology. The results of this study suggest that the regulation of reinforcing, modifying, or remodeling the structure of the transmitting tract and the pollen tube tip play a critical role in pollen-pistil interactions during pollen tube elongation in maize pistils, and the inhibition of these functions maybe the molecular mechanism of how Gametophytic factor1-Strong (Ga1-S) presence in style tissue completely prevents ga1 pollen to accomplish fertilization.