Endophytic fungi have been considered as a repertoire for bioactive secondary metabolites with potential application in medicine, agriculture and food industry. The biosynthetic pathways by fungal endophytes raise the argument of acquisition of these machineries of such complex metabolites from the plant host. Diterpenoids “Taxol” is the most effective anticancer drug with highest annual sale, since its discovery in 1970 from the Pacific yew tree, Taxus brevifolia. However, the lower yield of Taxol from this natural source (bark of T. brevifolia), availability and vulnerability of this plant to unpredicted fluctuation with the ecological and environmental conditions are the challenges. Endophytic fungi from Taxus spp. opened a new avenue for industrial Taxol production due to their fast growth, cost effectiveness, independence on climatic changes, feasibility of genetic manipulation. However, the anticipation of endophytic fungi for industrial Taxol production has been challenged by the loss of its productivity, due to the metabolic reprograming of cells, downregulating the expression of its encoding genes with subculturing and storage. Thus, the objectives of this review were to (1) Nominate the endophytic fungal isolates with the Taxol producing potency from Taxaceae and Podocarpaceae; (2) Emphasize the different approaches such as molecular manipulation, cultural optimization, co-cultivation for enhancing the Taxol productivities; (3) Accentuate the genome mining of the rate-limiting enzymes for rapid screening the Taxol biosynthetic machinery; (4) Triggering the silenced rate-limiting genes and transcriptional factors to activates the biosynthetic gene cluster of Taxol.

Silver ions discharged from various industries, are potentially toxic to living organisms at low concentrations, thus, there is an increasing need for development of an eco-friendly and cost-effective approach for its bioremediation. Filamentous fungi especially, Fusarium solani displayed a strong resistance to copper and cadmium ions as revealed from our previous study (El-Sayed 2014), however, the mechanisms of silver resistance by this fungus has not been resolved yet. Thus, this study was an extension to our previous work, to elucidate the mechanism of silver ions resistance and biotransformation by F. solani. The growth, bioaccumulation, thiol, total antioxidant, malondialdehyde (MDA), hydrogen peroxide (H₂O₂) contents and polyphenol oxidase (PPO) and catalase (CAT) activities of F. solani in response to silver ions were determined. Production and bioaccumulation of silver nanoparticles was characterized by UV-visible spectroscopy, TEM, and X-ray powder diffraction (XRD). The ultrastructural changes of F. solani induced by Ag(I) was examined by TEM and SEM. Production of oxalic acid by F. solani was increased by about 343.8% in response to 400 mg/l Ag(I), compared to control cultures (without silver ions) as revealed from HPLC analysis. The maximum biosorption levels by the native and alkali-treated biomass were carried out at pH 5.0, initial metal concentration 200 mg/l, biomass 0.5 g/l, temperature 35 °C, and contact time 1 h (native biomass) and 3 h (alkali-treated biomass). Fourier transform infrared spectroscopy (FTIR) results revealed that the main functional groups involved on this mycoremediation were C-S stretching, C=O C=N, C - H bending, C-N stretching and N-H bending. EDX spectra indicated the involvement of fungal cellular sulfur and phosphorus compounds in Ag(I) binding.