Feeding a rising population of currently 7.8 billion people globally requires efficient agriculture, which is preferably sustainable. Today, farmers are largely dependent on synthetic fungicides to avoid food losses caused by fungal diseases. However, the extensive use of these has resulted in the emergence of fungicide-resistant pathogens and concerns have been raised over the residual effects on the environment and human health. In this regard, biocontrol agents (BCAs) have been proposed as an alternative to standard fungicides but their disease management capacity is usually incomplete and heavily relies on uncontrollable environmental conditions. An integrated approach combining BCAs with fungicides, which is the focus of this review, is put forward as a way to reduce the fungicide doses to manage plant diseases and thereby their residue on harvested crops. In addition, such a strategy of combining antifungal treatments with different modes of action reduces the selection pressure on pathogens and thereby the chances of resistance development. However, to allow its large-scale implementation, further knowledge is needed, comprising timing, number and interval of repeated BCA applications and their compatibility with fungicides. The compatibility of BCAs with fungicides might differ when applied in a mixture or when used in alternation.

Peptides fulfill a plethora of functions in plant growth, development, and stress responses. They act as key components of cell-to-cell communication, interfere with signaling and response pathways, or display antimicrobial activity. Strikingly, both the diversity and amount of plant peptides have been largely underestimated. Most characterized plant peptides to date acting as small signaling peptides or antimicrobial peptides are derived from nonfunctional precursor proteins. However, evidence is emerging on peptides derived from a functional protein, directly translated from small open reading frames (without the involvement of a precursor) or even encoded by primary transcripts of microRNAs. These novel types of peptides further add to the complexity of the plant peptidome, even though their number is still limited and functional characterization as well as translational evidence are often controversial. Here, we provide a comprehensive overview of the reported types of plant peptides, including their described functional and structural properties. We propose a novel, unifying peptide classification system to emphasize the enormous diversity in peptide synthesis and consequent complexity of the still expanding knowledge on the plant peptidome.

Plant defensins are small, cysteine-rich peptides that possess biological activity towards a broad range of organisms. Their activity is primarily directed against fungi, but bactericidal and insecticidal actions have also been reported. The mode of action of various antifungal plant defensins has been studied extensively during the last decades and several of their fungal targets have been identified to date. This review summarizes the mechanism of action of well-characterized antifungal plant defensins, including RsAFP2, MsDef1, MtDef4, NaD1 and Psd1, and points out the variety by which antifungal plant defensins affect microbial cell viability. Furthermore, this review summarizes production routes for plant defensins, either via heterologous expression or chemical synthesis. As plant defensins are generally considered non-toxic for plant and mammalian cells, they are regarded as attractive candidates for further development into novel antimicrobial agents.