The increasing need for effective analytical tools to evaluate beef quality has prompted the development of new procedures to improve the animal sector’s performance. In this study, three beef breeds—Thai native (TN), crossbred Brahman × Thai native (BT), and crossbred Charolais × Brahman (CB)—were compared in terms of their physicochemical and metabolic profiles. The findings demonstrated that TN beef was lighter and tougher than other beef. Beef odor was stronger in BT. In addition, CB beef was the most tender and had the highest intramuscular fat content. Twenty-one different metabolites were found overall through NMR and chemometric approaches. The primary factors contributing to the difference in OPLS-DA loading plots were acetylcholine, valine, adenine, leucine, phosphocreatine, β-hydroxypyruvate, ethanol, adenosine diphosphate, creatine, acetylcholine, and lactate. The multivariate analysis indicated that these metabolites in beef cattle breeds could be distinguished using NMR spectroscopy. The results of this study provide valuable information on the quality and meat metabolites of different breeds. This could help in the development of a more accurate assessment of the quality of beef in future research.

The increasing need for effective analytical tools to evaluate beef quality has prompted the development of new procedures to improve the animal sector’s performance. In this study, three beef breeds—Thai native (TN), crossbred Brahman x Thai native (BT), and crossbred Charolais x Brahman (CB)—were compared in terms of their physicochemical and metabolic profiles. The findings demonstrated that TN beef was lighter and tougher than other beef. Beef odor was stronger in BT. In addition, CB beef was the most tender and the highest intramuscular fat content. Twenty-one different metabolites were found overall through NMR and chemometric approaches. High levels of lactate and creatine were found in all species. The primary factors contributing to the difference in OPLS-DA loading plots were acetylcholine, valine, adenine, leucine, and phosphocreatine, β-hydroxypyruvate, ethanol, adenosine diphosphate, creatine, acetylcholine, and lactate. The multivariate analysis indicated that these metabolites in beef cattle breeds could be distinguished using NMR spectroscopy. The results of this study provide valuable information on the quality and meat metabolites in different breeds. This could help in the development of a more accurate assessment of the quality of beef in future research.

Rising concerns about antimicrobial resistance and the safety of livestock-derived products have increased interest in natural feed additives for poultry production. Pineapple (Ananas comosus), which is rich in bromelain and phenolic compounds, has emerged as a promising candidate for supporting digestive function. This study evaluated the effects of concentrated pineapple extract (CPE) on nutrient digestibility, growth performance, and gastrointestinal health in broiler chickens. Using a completely randomized 6 × 2 factorial arrangement, 288 one-day-old Arbor Acres chicks were distributed to six dietary treatments (CPE at 0, 5, 10, 15, and 20 mL/kg, and a 110 ppm chlortetracycline), with six replications. Chicks were fed ad libitum for 42 days, and growth performance, apparent total tract nutrient disappearance, gut pH, microbial populations, and intestinal histomorphology were assessed. No significant diet–sex interaction was observed. CPE supplementation reduced gastric and duodenal pH and feed intake but did not affect body weight or intestinal morphology. Notably, CPE improved apparent total tract N retention and reduced intestinal Escherichia coli counts, while supplementation at 5 mL/kg enhanced fat digestibility. Sex-related differences were observed in jejunum and ileum pH. Overall, dietary inclusion of 5–10 mL/kg (44,770–89,540 GDU/kg diet) CPE may support digestive efficiency without compromising growth performance in broiler chickens.