Soil microorganisms play key roles in biogeochemical cycling in forest ecosystems. However, whether the responses of microbial community with stand development differed in rhizosphere and bulk soils remains unknown. We collected rhizosphere and bulk soil in Chinese fir plantations with different stand ages (7a, 15a, 24a, and 34a) in subtropical China, and determined bacterial and fungal community variation via high-throughput sequencing. The results showed that soil bacterial, but not fungal, community diversity significantly differed among stand ages and between rhizosphere and bulk soils (p < 0.05). The differences in Shannon–Wiener and Simpson’s indices between rhizosphere and bulk soil varied with stand age, with significant higher soil bacterial diversity in rhizosphere than bulk soils in 7a and 34a plantations (p < 0.05), but there were no significant difference in soil bacterial diversity between rhizosphere and bulk soils in 15a and 24a plantations (p > 0.05). Soil microbial community composition varied significantly with stand age but not between the rhizosphere and bulk soil. The dominant bacterial phyla at all ages were Acidobacteria and Proteobacteria, while the dominant fungal phyla were Ascomycota and Basidiomycota in both rhizosphere and bulk soil. They showed inconsistent distribution patterns along stand age gradient (7–34a) in the rhizosphere and bulk soil, suggesting distinct ecological strategy (r-strategist vs. k-strategist) of different microbial taxa, as well as changes in the microenvironment (i.e., nutrient stoichiometry and root exudates). Moreover, bacterial and fungal community composition in rhizosphere and bulk soil were governed by distinct driving factors. TP and NH₄⁺–N are the two most important factors regulating bacterial and fungal community structure in rhizosphere soil, while pH and NO₃^––N, DON, and TN were driving factors for bacterial and fungal community structure in bulk soil, respectively. Collectively, our results demonstrated that the changes in microbial diversity and composition were more obvious along stand age gradients than between sampling locations (rhizosphere vs. bulk soil) in Chinese fir plantations.

Biochar is produced by burning biomass under oxygen‐limited conditions, and it has been widely used as a soil amendment to improve soil functions such as nutrient retention. However, whether the impact of biochar application on soil nitrogen (N) transformation and N₂O emission varies with the pyrolysis temperature remains unclear, especially in different forest types in subtropical regions. In this study, a 60‐day laboratory incubation experiment was conducted to evaluate the impact of biochar with different pyrolysis temperatures (300°C [BC300], 500°C [BC500], and 800°C [BC800]) on net N transformation rates and N₂O emission in soils collected from Castanopsis kawakamii dominated natural forest (NF) and Chinese fir (Cunninghamia lanceolate, CF) plantation in subtropical China. The results showed that the application of biochar significantly increased soil ammonium (NH₄⁺) content (p < 0.001) but reduced nitrate (NO₃⁻) content (p < 0.001) compared with the control. The soil NH₄⁺ content of the BC800 treatment was significantly higher than that of other treatments (p < 0.001). Biochar application significantly reduced soil net N mineralization (NR_min) and nitrification (NR_nit) rate (p < 0.001), but increased net ammonification (NR_amm) rate (p < 0.001). The application of biochar led to a remarkable decrease in cumulative N₂O emission compared to the control (p < 0.001). In particular, soils treated with high‐temperature biochar emitted significantly lower N₂O compared to other treatments (p < 0.001). The partial least squares path model demonstrated that biochar influenced N₂O emission through a direct effect in NF soil and an indirect effect in CF soil. This study highlights the distinct role of biochar, particularly that produced under high pyrolysis temperatures as a soil amendment to mitigate N₂O emission and promote N retention in both subtropical natural and planted forests.

Charcoal, a byproduct resulting from incomplete combustion of biomass in fire events, can modify the physical properties of soil due to its high porosity and large surface area. To evaluate the impact of fire-deposited charcoal on soil hydraulic characteristics, soil–charcoal mixtures were analyzed to investigate the effects of different application doses (wt%: 0, 1%, 3%, 5%, 10% and 20%) of charcoal on soil bulk density (BD), porosity (total, capillary, and non-capillary), residual moisture after free drainage (RM), saturated water content (SC), and saturated hydraulic conductivity (Ks) of loamy and sandy soils collected from subtropical forests in south China. The results showed that the impact of charcoal on soil’s physical and hydraulic properties depends on the soil type and the application dose. The incorporation of charcoal significantly decreased the BD of sandy soil (p < 0.001), while a significant decrease in BD in loamy soil was only observed as a result of the higher application doses (10% and 20%) (p < 0.001). Charcoal application doses of 5% or higher led to a significant increase in the total porosity (TP) of sandy soil (p < 0.001) and doses of 3% and 20% resulted in a significant increase in the TP of loamy soil (p < 0.001). The capillary porosity (CP) of both sand and loamy soils significantly increased when charcoal was applied at doses of 3% or higher (p < 0.001). The minimum charcoal application dose that significantly increased the RM in sandy soil was 5%, while for loamy soil, the minimum effective dose was 10%. Charcoal applied at a dose of 3% significantly increased the Ks of sandy soil (p < 0.001), while no significant effect on Ks was observed for loamy soil (p > 0.05). Collectively, our findings suggest that fire-derived charcoal enhances the soil water-retention capacity in subtropical forests, with the effects becoming more pronounced at higher application doses and being particularly notable in sandy soil compared to loamy soil.