Grapes, particularly the species Vitis vinifera L., are one of the most widely grown crops in the world. Winemaking processes generate a high amount of residues, which currently pose an environmental and economic sustainability problem for companies in the sector. For this reason, solutions are being explored for the development of new products with high-added value derived from the valorization of these residues. One of the wastes produced by winemaking processes is grape pomace, which chemical composition is promising because it is rich in compounds with high antioxidant activity, such as polyphenols (anthocyanins, flavonols, flavan-3-ols, procyanidins), phenolic acids, resveratrol, and fiber. Commonly grape pomace is used to produce distillates and to extract tartaric acid and coloring substances such as enocyanin. Recently, alternative uses of grape pomace have been adopted, such as the production of extracts with antioxidant properties, fermentation substrates, composting and biomass for energy production, and fiber extraction for the development of high-value-added products enriched with bioactive molecules from grape pomace. Here, we discuss how bioactive molecules from grape pomace are involved in various human biological functions and their applications in the agri-food sector.

Grape pomace (GP), a major byproduct obtained from the winemaking process, is characterized by a high amount of phenolic compounds and secondary plant metabolites, with potential beneficial effects on human health. Therefore, GP is a source of bioactive compounds with antioxidant, antimicrobial, and anti-inflammatory activity. As people are paying more attention to sustainability, in this work, we evaluate two different extractions (aqueous and hydroalcoholic) of GP bioactive compounds. In vitro simulated gastrointestinal digestion of the GP extracts was performed to improve the bioavailability and bioaccessibility of polyphenols. The antioxidant activity (ABTS and DPPH assays) and the phenolic characterization of the extracts by UHPLC-DAD were evaluated. The antimicrobial effects of GP antioxidants in combination with a probiotic (Lactiplantibacillus plantarum) on the growth of pathogenic microorganisms (Escherichia coli, Bacillus megaterium, and Listeria monocytogenes) were evaluated. As a result, an increase of antioxidant activity of aqueous GP extracts during the gastrointestinal digestion, and a contextual decrease of hydroalcoholic extracts, were detected. The main compounds assessed by UHPLC-DAD were anthocyanins, phenolic acids, flavonoids, and stilbenes. Despite lower antioxidant activity, due to the presence of antimicrobial active compounds, the aqueous extracts inhibited the growth of pathogens.

TThe popularity of pomegranate (Punica granatum L.) is increasing among growers and consumers due toits nutritional value, nutraceutical properties and adaptability to different soils and climate conditions.Pomegranate is characterized by a large variability in terms of domestic, wild and ornamental genotypes,and for seed-hardness, juiciness, rind and aril color, sugar/acid ratio, antioxidant activity, fatty acids, phe-nols and arils anthocyanins content. Evaluation of pomegranate germplasm has usually been performedusing fruit morphological and chemical traits, whereas genetic studies received less attention. The aimof the present work was: (a) to carry on a morphological and biochemical characterization of a set ofpomegranate genotypes collected in Puglia region, Southeastern Italy and (b) to investigate genetic diver-sity by microsatellite markers. A two-year study was carried out for the evaluation of 13 pomegranategenotypes, sweet and sour, of Italian and Israeli origin. Significant differences were observed for many ofthe parameters investigated, in particular fruit and aril size and maturity index of fruits, titratable acidity,total polyphenol content and antioxidant activity of juices and oil content of seeds. Genetic analysis on thepomegranate collection identified 53 SSR loci able to distinguish the different genotypes and to estimategenetic distances. In the dendrogram, pomegranate genotypes clustered according to both geographicalorigin and morphological–chemical traits (skin color, size, taste and polyphenolic content), suggestingthat SSR markers could be effective to detect the genetic diversity of pomegranate, thus facilitating theexploitation of this fruit species germplasm. In general, morphological, chemical and genetic parametersallowed to clearly distinguish the Italian genotypes from the Israeli ones