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Recently, Menzel A et al. published a review titled “Origin and Physiological Aspects of Oxidative Stress (OS), inflammation and markers of OS, relation to disease and practical aspects”
Simona Mrakic-Sposta; Maristella Gussoni; Michela Montorsi; Alessandra Vezzoli. Comment on Menzel et al. Common and Novel Markers for Measuring Inflammation and Oxidative Stress Ex Vivo in Research and Clinical Practice—Which to Use Regarding Disease Outcomes? Antioxidants 2021, 10, 414. Antioxidants 2021, 10, 836 .
AMA StyleSimona Mrakic-Sposta, Maristella Gussoni, Michela Montorsi, Alessandra Vezzoli. Comment on Menzel et al. Common and Novel Markers for Measuring Inflammation and Oxidative Stress Ex Vivo in Research and Clinical Practice—Which to Use Regarding Disease Outcomes? Antioxidants 2021, 10, 414. Antioxidants. 2021; 10 (6):836.
Chicago/Turabian StyleSimona Mrakic-Sposta; Maristella Gussoni; Michela Montorsi; Alessandra Vezzoli. 2021. "Comment on Menzel et al. Common and Novel Markers for Measuring Inflammation and Oxidative Stress Ex Vivo in Research and Clinical Practice—Which to Use Regarding Disease Outcomes? Antioxidants 2021, 10, 414." Antioxidants 10, no. 6: 836.
Conjugated polymers with ionic pendant groups (CPEs) are receiving increasing attention as solution-processed interfacial materials for organic solar cells (OSCs). Various anionic CPEs have been successfully used, on top of ITO (Indium Tin Oxide) electrodes, as solution-processed anode interlayers (AILs) for conventional devices with direct geometry. However, the development of CPE AILs for OSC devices with inverted geometry is an important topic that still needs to be addressed. Here, we have designed three anionic CPEs bearing alkyl-potassium-sulfonate side chains. Their functional behavior as anode interlayers has been investigated in P3HT:PC61BM (poly(3-hexylthiophene): [6,6]-phenyl C61 butyric acid methyl ester) devices with an inverted geometry, using a hole collecting silver electrode evaporated on top. Our results reveal that to obtain effective anode modification, the CPEs’ conjugated backbone has to be tailored to grant self-doping and to have a good energy-level match with the photoactive layer. Furthermore, the sulfonate moieties not only ensure the solubility in polar orthogonal solvents, induce self-doping via a right choice of the conjugated backbone, but also play a role in the gaining of hole selectivity of the top silver electrode.
Elisa Lassi; Benedetta Squeo; Roberto Sorrentino; Guido Scavia; Simona Mrakic-Sposta; Maristella Gussoni; Barbara Vercelli; Francesco Galeotti; Mariacecilia Pasini; Silvia Luzzati. Sulfonate-Conjugated Polyelectrolytes as Anode Interfacial Layers in Inverted Organic Solar Cells. Molecules 2021, 26, 763 .
AMA StyleElisa Lassi, Benedetta Squeo, Roberto Sorrentino, Guido Scavia, Simona Mrakic-Sposta, Maristella Gussoni, Barbara Vercelli, Francesco Galeotti, Mariacecilia Pasini, Silvia Luzzati. Sulfonate-Conjugated Polyelectrolytes as Anode Interfacial Layers in Inverted Organic Solar Cells. Molecules. 2021; 26 (3):763.
Chicago/Turabian StyleElisa Lassi; Benedetta Squeo; Roberto Sorrentino; Guido Scavia; Simona Mrakic-Sposta; Maristella Gussoni; Barbara Vercelli; Francesco Galeotti; Mariacecilia Pasini; Silvia Luzzati. 2021. "Sulfonate-Conjugated Polyelectrolytes as Anode Interfacial Layers in Inverted Organic Solar Cells." Molecules 26, no. 3: 763.
The effects of two different dietary supplements on the redox status of healthy human participants were evaluated. The first supplement (GluS, Glutathione Synthesis) contains the precursors for the endogenous synthesis of glutathione and the second (GluReS, Glutathione and Resveratrol Synthesis) contains in addition polydatin, a precursor of resveratrol. To assess the influence of GluS and GluReS on the redox status, ten thiol species and three vitamins were measured before (t0) and after 8 weeks (t1) of dietary supplementation. An inflammatory marker, neopterin, was also assessed at the same time points. Both supplements were highly effective in improving the redox status by significantly increasing the reduced-glutathione (GSH) content and other reduced thiol species while significantly decreasing the oxidized species. The positive outcome of the redox status was most significant in the GluRes treatment group which also experienced a significant reduction in neopterin levels. Of note, the endogenous levels of vitamins C, E and A were significantly increased in both treatment groups, with best results in the GluReS group. While both dietary supplements significantly contributed to recognized antioxidant and anti-inflammatory outcomes, the effects of GluReS, the combination of glutathione and resveratrol precursors, were more pronounced. Thus, dietary supplementation with GluReS may represent a valuable strategy for maintaining a competent immune status and a healthy lifespan.
Priscilla Biswas; Cinzia Dellanoce; Alessandra Vezzoli; Simona Mrakic-Sposta; Mauro Malnati; Alberto Beretta; Roberto Accinni. Antioxidant Activity with Increased Endogenous Levels of Vitamin C, E and A Following Dietary Supplementation with a Combination of Glutathione and Resveratrol Precursors. Nutrients 2020, 12, 3224 .
AMA StylePriscilla Biswas, Cinzia Dellanoce, Alessandra Vezzoli, Simona Mrakic-Sposta, Mauro Malnati, Alberto Beretta, Roberto Accinni. Antioxidant Activity with Increased Endogenous Levels of Vitamin C, E and A Following Dietary Supplementation with a Combination of Glutathione and Resveratrol Precursors. Nutrients. 2020; 12 (11):3224.
Chicago/Turabian StylePriscilla Biswas; Cinzia Dellanoce; Alessandra Vezzoli; Simona Mrakic-Sposta; Mauro Malnati; Alberto Beretta; Roberto Accinni. 2020. "Antioxidant Activity with Increased Endogenous Levels of Vitamin C, E and A Following Dietary Supplementation with a Combination of Glutathione and Resveratrol Precursors." Nutrients 12, no. 11: 3224.
S Mrakic-Sposta; M Gussoni; C Dellanoce; M Marzorati; M Montorsi; L Rasica; L Pratali; G D'angelo; M Martinelli; L Bastiani; L Di Natale; A Vezzoli. Effects of acute and sub-acute hypobaric hypoxia on oxidative stress: a field study in the Alps. 2020, 1 .
AMA StyleS Mrakic-Sposta, M Gussoni, C Dellanoce, M Marzorati, M Montorsi, L Rasica, L Pratali, G D'angelo, M Martinelli, L Bastiani, L Di Natale, A Vezzoli. Effects of acute and sub-acute hypobaric hypoxia on oxidative stress: a field study in the Alps. . 2020; ():1.
Chicago/Turabian StyleS Mrakic-Sposta; M Gussoni; C Dellanoce; M Marzorati; M Montorsi; L Rasica; L Pratali; G D'angelo; M Martinelli; L Bastiani; L Di Natale; A Vezzoli. 2020. "Effects of acute and sub-acute hypobaric hypoxia on oxidative stress: a field study in the Alps." , no. : 1.
Saturation diving allows divers to reduce the risk of decompression sickness while working at depth for prolonged periods but may increase reactive oxygen species (ROS) production. Such modifications can affect endothelial function by exacerbating oxidative stress. This study investigated the effects of saturation diving on oxidative stress damage. Redox status was evaluated through: ROS production; total antioxidant capacity (TAC); nitric oxide metabolites (NOx); nitrotyrosine (3-NT); and lipid peroxidation (8-iso-PGF2α) assessment. Creatinine and neopterin were analyzed as markers of renal function and damage. Measurements were performed on saliva and urine samples obtained at four time points: pre; deep; post; and 24 h post. Four divers were included in the study. After the saturation dive (post), significant (p < 0.05) increases in ROS (0.12 ± 0.03 vs. 0.36 ± 0.06 µmol.min−1), TAC (1.88 ± 0.03 vs. 2.01 ± 0.08 mM), NOx (207.0 ± 103.3 vs. 441.8 ± 97.3 µM), 3-NT (43.32 ± 18.03 vs. 18.64 ± 7.45 nM·L−1), and 8-iso-PGF2α (249.7 ± 45.1 vs. 371.9 ± 54.9 pg·mg−1 creatinine) were detected. Markers of renal damage were increased as well after the end of the saturation dive (creatinine 0.54 ± 0.22 vs. 2.72 ± 1.12 g-L−1; neopterin 73.3 ± 27.9 vs. 174.3 ± 20.53 μmol·mol−1 creatinine). These results could ameliorate commercial or military diving protocols or improve the understanding of symptoms caused by oxygen level elevation.
Simona Mrakic-Sposta; Alessandra Vezzoli; Federica D’Alessandro; Matteo Paganini; Cinzia Dellanoce; Danilo Cialoni; Gerardo Bosco. Change in Oxidative Stress Biomarkers During 30 Days in Saturation Dive: A Pilot Study. International Journal of Environmental Research and Public Health 2020, 17, 7118 .
AMA StyleSimona Mrakic-Sposta, Alessandra Vezzoli, Federica D’Alessandro, Matteo Paganini, Cinzia Dellanoce, Danilo Cialoni, Gerardo Bosco. Change in Oxidative Stress Biomarkers During 30 Days in Saturation Dive: A Pilot Study. International Journal of Environmental Research and Public Health. 2020; 17 (19):7118.
Chicago/Turabian StyleSimona Mrakic-Sposta; Alessandra Vezzoli; Federica D’Alessandro; Matteo Paganini; Cinzia Dellanoce; Danilo Cialoni; Gerardo Bosco. 2020. "Change in Oxidative Stress Biomarkers During 30 Days in Saturation Dive: A Pilot Study." International Journal of Environmental Research and Public Health 17, no. 19: 7118.
Hyperhomocysteinemia is recognized as risk factor for cardiovascular and age-associated diseases. Folic acid supplementation efficiently lowers plasma homocysteine (Hcy) levels, but high intake may negatively affect health because of unnatural levels of unmetabolized folic acid in the systemic circulation. Oxoproline (Oxo) provides by glutamic acid production an increase of intracellular folic acid trapping. Aim of this study was to compare the efficacy of three supplementation protocols: (1) traditional therapy (5-methyl-tetrahydrofolate: 15 mg/day); (2) 5 mL/day of Oxo with 300 μg folic acid (oxifolic); (3) 5 mL/day of Oxo alone (magnesio+) in a 90 days randomized trial on thirty-two moderate hyperhomocysteinemic (18.6 ± 2.4 μmol.L−1) patients (age 48 ± 14 yrs). Thiols: cysteine (Cys), cysteinylglycine (Cys–Gly) and glutathione levels were assessed too. Every supplementation induced significant (p range p < 0.01) and traditional (p < 0.05) supplementation. The integration of Oxo resulted an interesting alternative to traditional therapy because absence or minimal number of folates in the integrator eliminates any chance of excess that can constitute a long-term risk.
Alessandra Vezzoli; Cinzia Dellanoce; Teresa Maria Caimi; Daniele Vietti; Michela Montorsi; Simona Mrakic-Sposta; Roberto Accinni. Influence of Dietary Supplementation for Hyperhomocysteinemia Treatments. Nutrients 2020, 12, 1957 .
AMA StyleAlessandra Vezzoli, Cinzia Dellanoce, Teresa Maria Caimi, Daniele Vietti, Michela Montorsi, Simona Mrakic-Sposta, Roberto Accinni. Influence of Dietary Supplementation for Hyperhomocysteinemia Treatments. Nutrients. 2020; 12 (7):1957.
Chicago/Turabian StyleAlessandra Vezzoli; Cinzia Dellanoce; Teresa Maria Caimi; Daniele Vietti; Michela Montorsi; Simona Mrakic-Sposta; Roberto Accinni. 2020. "Influence of Dietary Supplementation for Hyperhomocysteinemia Treatments." Nutrients 12, no. 7: 1957.
The response to strenuous exercise was investigated by reactive oxygen species (ROS) production, oxidative damage, thiol redox status, and inflammation assessments in 32 enrolled triathlon athletes (41.9±7.9 yrs) during Ironman® (IR), or half Ironman® (HIR) competition. In biological samples, inflammatory cytokines, aminothiols (glutathione (GSH), homocysteine (Hcy), cysteine (Cys), and cysteinylglycine (CysGly)), creatinine and neopterin, oxidative stress (OxS) biomarkers (protein carbonyl (PC), thiobarbituric acid-reactive substances (TBARS)), and ROS were assessed. Thirteen HIR and fourteen IR athletes finished the race. Postrace, ROS (HIR +20%; IR +28%; p<0.0001), TBARS (HIR +57%; IR +101%), PC (HIR +101%; IR +130%) and urinary neopterin (HIR +19%, IR +27%) significantly (range p<0.05-0.0001) increased. Moreover, HIR showed an increase in total Cys +28%, while IR showed total aminothiols, Cys, Hcy, CysGly, and GSH increase by +48, +30, +58, and +158%, respectively (range p<0.05-0.0001). ROS production was significantly correlated with TBARS and PC (R2=0.38 and R2=0.40; p<0.0001) and aminothiols levels (range R2=0.17-0.47; range p<0.01-0.0001). In particular, ROS was directly correlated with the athletes’ age (R2=0.19; p<0.05), with ultraendurance years of training (R2=0.18; p<0.05) and the days/week training activity (R2=0.16; p<0.05). Finally, the days/week training activity (hours/in the last 2 weeks) was found inversely correlated with the IL-6 postrace (R2=‐0.21; p<0.01). A strenuous performance, the Ironman® distance triathlon competition, alters the oxidant/antioxidant balance through a great OxS response that is directly correlated to the inflammatory parameters; furthermore, the obtained data suggest that an appropriate training time has to be selected in order to achieve the lowest ROS production and IL-6 concentration at the same time.
Simona Mrakic-Sposta; Maristella Gussoni; Alessandra Vezzoli; Cinzia Dellanoce; Mario Comassi; Guido Giardini; Rosa Maria Bruno; Michela Montorsi; Anca Corciu; Fulvia Greco; Lorenza Pratali. Acute Effects of Triathlon Race on Oxidative Stress Biomarkers. Oxidative Medicine and Cellular Longevity 2020, 2020, 1 -14.
AMA StyleSimona Mrakic-Sposta, Maristella Gussoni, Alessandra Vezzoli, Cinzia Dellanoce, Mario Comassi, Guido Giardini, Rosa Maria Bruno, Michela Montorsi, Anca Corciu, Fulvia Greco, Lorenza Pratali. Acute Effects of Triathlon Race on Oxidative Stress Biomarkers. Oxidative Medicine and Cellular Longevity. 2020; 2020 ():1-14.
Chicago/Turabian StyleSimona Mrakic-Sposta; Maristella Gussoni; Alessandra Vezzoli; Cinzia Dellanoce; Mario Comassi; Guido Giardini; Rosa Maria Bruno; Michela Montorsi; Anca Corciu; Fulvia Greco; Lorenza Pratali. 2020. "Acute Effects of Triathlon Race on Oxidative Stress Biomarkers." Oxidative Medicine and Cellular Longevity 2020, no. : 1-14.
Exposure to high altitude is one of the most widely used models to study the adaptive response to hypoxia in humans. However, little is known about the related effects on micturition. The present study addresses the adaptive urinary responses in four healthy adult lowlanders, comparing urodynamic indexes at Kathmandu [1,450 m above sea level (a.s.l.); K1450] and during a sojourn in Namche Bazar (3,500 m a.s.l.; NB3500). The urodynamic testing consisted of cistomanometry and bladder pressure/flow measurements. Anthropometrics, electrocardiographic, and peripheral capillary oxygen saturation data were also collected. The main findings consisted of significant reductions in bladder power at maximum urine flow by ~30%, bladder contractility index by 13%, and infused volume both at first (by 57%) and urgency sensation (by 14%) to urinate, indicating a reduced cystometric capacity, at NB3500. In addition to the urinary changes, we found that oxygen saturation, body mass index, body surface area, and median RR time were all significantly reduced at altitude. We submit that the hypoxia-related parasympathetic inhibition could be the underlying mechanism of both urodynamic and heart rate adaptive responses to high-altitude exposure. Moreover, increased diuresis and faster bladder filling at altitude may trigger the anticipation of being able to void, a common cause of urgency. We believe that the present pilot study represents an original approach to the study of urinary physiology at altitude.
Vittore Verratti; Simona Mrakic-Sposta; Manuela Moriggi; Alessandro Tonacci; Suwas Bhandari; Danilo Migliorelli; Ashok Bajracharya; Danilo Bondi; Enrico Finazzi Agrò; Paolo Cerretelli. Urinary physiology and hypoxia: a pilot study of moderate-altitude trekking effects on urodynamic indexes. American Journal of Physiology-Renal Physiology 2019, 317, F1081 -F1086.
AMA StyleVittore Verratti, Simona Mrakic-Sposta, Manuela Moriggi, Alessandro Tonacci, Suwas Bhandari, Danilo Migliorelli, Ashok Bajracharya, Danilo Bondi, Enrico Finazzi Agrò, Paolo Cerretelli. Urinary physiology and hypoxia: a pilot study of moderate-altitude trekking effects on urodynamic indexes. American Journal of Physiology-Renal Physiology. 2019; 317 (4):F1081-F1086.
Chicago/Turabian StyleVittore Verratti; Simona Mrakic-Sposta; Manuela Moriggi; Alessandro Tonacci; Suwas Bhandari; Danilo Migliorelli; Ashok Bajracharya; Danilo Bondi; Enrico Finazzi Agrò; Paolo Cerretelli. 2019. "Urinary physiology and hypoxia: a pilot study of moderate-altitude trekking effects on urodynamic indexes." American Journal of Physiology-Renal Physiology 317, no. 4: F1081-F1086.
An innovative moderate-intensity resistive exercise-training (RT) program was tested in thirty-five sarcopenic elders (SAR). The subjects were randomized into two groups: SAR training (SAR-RT), n = 20, 73.0 ± 5.5 years, or SAR non-training (SAR-NT), n = 15, 71.7 ± 3.4 years. The training consisted of 12-week progressive RT, thrice/week, at 60% one-repetition maximum (1RM), 3 sets, 14–16 repetitions for both upper and lower limbs. The pre and post intervention measurements included: the skeletal muscle index (SMI%); strength (1RM); stair-climbing power (SCP); muscle thickness (MT) of vastus lateralis (VL) and elbow flexors (EF), VL pennation angle (PA), rectus femoris (RF) anatomical cross-sectional area (ACSA); reactive oxygen species (ROS), total antioxidant capacity (TAC), protein carbonyls (PC), thiobarbituric acid-reactive substances (TBARS), 8-isoprostane (8-iso-PGF2-α), 8-OH-2-deoxyguanosine (8-OH-dG), as markers of oxidative stress/damage (OxS). In SAR-RT, SCP increased by 7.7% (P < 0.01), MT increased by 5.5% for VL, 10.4% for EF and PA increased by 13.4% for VL (P < 0.001 for all). The RF ACSA increased by 14.5% (P < 0.001). 1RM significantly increased by at least 67% for all muscles tested. Notably muscle strength (1RM) positively correlated (P < 0.001) with TAC and negatively with PC (P < 0.001). In conclusion, moderate intensity RT is an effective strategy to increase muscle mass and strength in SAR, while minimizing OxS.
Alessandra Vezzoli; Simona Mrakic-Sposta; Michela Montorsi; Simone Porcelli; Paola Vago; Ferdinando Cereda; Stefano Longo; Marcello Maggio; Marco Narici; Mrakic- Sposta; Vago. Moderate Intensity Resistive Training Reduces Oxidative Stress and Improves Muscle Mass and Function in Older Individuals. Antioxidants 2019, 8, 431 .
AMA StyleAlessandra Vezzoli, Simona Mrakic-Sposta, Michela Montorsi, Simone Porcelli, Paola Vago, Ferdinando Cereda, Stefano Longo, Marcello Maggio, Marco Narici, Mrakic- Sposta, Vago. Moderate Intensity Resistive Training Reduces Oxidative Stress and Improves Muscle Mass and Function in Older Individuals. Antioxidants. 2019; 8 (10):431.
Chicago/Turabian StyleAlessandra Vezzoli; Simona Mrakic-Sposta; Michela Montorsi; Simone Porcelli; Paola Vago; Ferdinando Cereda; Stefano Longo; Marcello Maggio; Marco Narici; Mrakic- Sposta; Vago. 2019. "Moderate Intensity Resistive Training Reduces Oxidative Stress and Improves Muscle Mass and Function in Older Individuals." Antioxidants 8, no. 10: 431.
Breath-hold diving results in significant changes in blood gases’ levels. Challenging variations in oxygen partial pressures may induce reactive oxygen species (ROS) production that exacerbate oxidative stress and, consequently, affect endothelial function. The aim of this study was to investigate the effects of breath-hold diving on oxidative stress damage, assessing ROS production. Nitric oxide metabolites, inducible nitric oxide synthase (iNOS), aminothiols, and renal function were evaluated too as markers of redox status and renal damage. ROS production was assessed with electron paramagnetic resonance. Oxidative status values were measured at pre- and post-40 m dive in a deep swimming pool (Y-40) from six divers (mean age 46.6 ± 9.3 years; height 176 ± 4 cm; BMI 25 ± 2.9 kg/m2). Significant (p < 0.05) increases at post-dive of ROS production rate (0.158 ± 0.003 vs 0.195 ± 0.006 μmol min−1), lipid peroxidation (8-isoprostane: 375.67 ± 195.62 vs 420.49 ± 232.31 pg mg−1 creatinine), nitrate (27.91 ± 19.71 vs 30.80 ± 20.44 μM), iNOS (31.30 ± 4.52 vs 35.68 ± 6.72 IU mL−1) and neopterin concentration (96.20 ± 40.41 vs 118.76 ± 27.84 μmol mol−1 creatinine) were recorded. Conversely, the antioxidant capacity significantly decreased (3.423 ± 0.089 vs 3.015 ± 0.284 mM) after immersion. Overproduction of ROS and consequent oxidative damage to lipids of membrane and antioxidant capacity decreasing reflect also a hypoxic condition, which in the breath-hold diving typically occurs in the last few meters below the surface. iNOS produces NO in large quantities under the examined extreme conditions. Neopterin and creatinine concentration level increased, suggesting an “impairment of renal function” as a likely physiological response to PaO2 variations during dive activity.
Simona Mrakic-Sposta; Alessandra Vezzoli; Alex Rizzato; Cinzia Della Noce; Sandro Malacrida; Michela Montorsi; Matteo Paganini; Pasqua Cancellara; Gerardo Bosco. Oxidative stress assessment in breath-hold diving. Graefe's Archive for Clinical and Experimental Ophthalmology 2019, 119, 2449 -2456.
AMA StyleSimona Mrakic-Sposta, Alessandra Vezzoli, Alex Rizzato, Cinzia Della Noce, Sandro Malacrida, Michela Montorsi, Matteo Paganini, Pasqua Cancellara, Gerardo Bosco. Oxidative stress assessment in breath-hold diving. Graefe's Archive for Clinical and Experimental Ophthalmology. 2019; 119 (11-12):2449-2456.
Chicago/Turabian StyleSimona Mrakic-Sposta; Alessandra Vezzoli; Alex Rizzato; Cinzia Della Noce; Sandro Malacrida; Michela Montorsi; Matteo Paganini; Pasqua Cancellara; Gerardo Bosco. 2019. "Oxidative stress assessment in breath-hold diving." Graefe's Archive for Clinical and Experimental Ophthalmology 119, no. 11-12: 2449-2456.
High altitude is a natural laboratory, within which the clinical study of human physiological response to hypobaric hypoxia (HH) is possible. Failure in the response results in progressive hypoxemia, inflammation and increased tissue oxidative stress (OxS). Thus, investigating temporal changes in key transcription factors (TFs) HIF-1α, HIF-2α, NF-κB and NRF2 mRNA levels, relative to OxS and inflammatory markers, may reveal molecular targets which contrast deleterious effects of hypoxia. Biological samples and clinical data from 15 healthy participants were collected at baseline and after rapid, passive ascent to 3830 m (24 h and 72 h). Gene expression was assessed by qPCR and ROS generation was determined by EPR spectroscopy. Oxidative damage and cytokine levels were estimated by immuno or enzymatic methods. Hypoxia transiently enhanced HIF-1α mRNA levels over time reaching a peak after 24 h. Whereas, HIF-2α and NRF2 mRNA levels increased over time. In contrast, the NF-κB mRNA levels remained unchanged. Plasma levels of IL-1β and IL-6 also remained within normal ranges. ROS production rate and markers of OxS damage were significantly increased over time. The analysis of TF-gene expression suggests that HIF-1α is a lead TF during sub-acute HH exposure. The prolongation of the HH exposure led to a switch between HIF-1α and HIF-2α/NRF2, suggesting the activation of new pathways. These results provide new insights regarding the temporal regulation of TFs, inflammatory state, and ROS homeostasis involved in human hypoxic response, potentially also relevant to the mediation of diseases that induce a hypoxic state.
Sandro Malacrida; Alessandra Giannella; Giulio Ceolotto; Carlo Reggiani; Alessandra Vezzoli; Simona Mrakic-Sposta; Sarah Moretti; Rachel Turner; Marika Falla; Hermann Brugger; Giacomo Strapazzon. Transcription Factors Regulation in Human Peripheral White Blood Cells during Hypobaric Hypoxia Exposure: an in-vivo experimental study. Scientific Reports 2019, 9, 1 -13.
AMA StyleSandro Malacrida, Alessandra Giannella, Giulio Ceolotto, Carlo Reggiani, Alessandra Vezzoli, Simona Mrakic-Sposta, Sarah Moretti, Rachel Turner, Marika Falla, Hermann Brugger, Giacomo Strapazzon. Transcription Factors Regulation in Human Peripheral White Blood Cells during Hypobaric Hypoxia Exposure: an in-vivo experimental study. Scientific Reports. 2019; 9 (1):1-13.
Chicago/Turabian StyleSandro Malacrida; Alessandra Giannella; Giulio Ceolotto; Carlo Reggiani; Alessandra Vezzoli; Simona Mrakic-Sposta; Sarah Moretti; Rachel Turner; Marika Falla; Hermann Brugger; Giacomo Strapazzon. 2019. "Transcription Factors Regulation in Human Peripheral White Blood Cells during Hypobaric Hypoxia Exposure: an in-vivo experimental study." Scientific Reports 9, no. 1: 1-13.
Impaired glucose tolerance (IGT) is a risk factor for the development of diabetes and related complications that ensue. Early identification of at-risk individuals might be beneficial to reduce or delay the progression of diabetes and its related complications. Recently, microRNAs emerged as potential biomarkers of diseases. The aim of the present study was to evaluate microRNA-21 as a potential biomarker for the risk of developing diabetes in adults with IGT and to investigate its downstream effects as the generation of reactive oxygen species (ROS), the induction of manganese-superoxide dismutase-2 (SOD2), and the circulating levels of 4-HNE (4-hydroxynonenal). To evaluate the prognostic and predictive values of plasmatic microRNA-21 in identifying metabolic derangements, we tested a selected cohort (n = 115) of subjects enrolled in the DIAPASON Study, whom were selected on ADA criteria for 2hPG. Statistical analysis was performed using ANOVA or the Kruskal–Wallis test as appropriate. ROC curves were drawn for diagnostic accuracy of the tests; positive and negative predictive values were performed, and Youden’s index was used to seek the cut-off optimum truncation point. ROS, SOD2 and 4-HNE were also evaluated. We observed significant upregulation of microRNA-21 in IGT and in T2D subjects, and microRNA-21 was positively correlated with glycaemic parameters. Diagnostic performance of microRNA-21 was high and accurate. We detected significant overproduction of ROS by electron paramagnetic resonance (EPR), significant accumulation of the lipid peroxidation marker 4-HNE, and defective SOD2 antioxidant response in IGT and newly diagnosed, drug-naïve T2D subjects. In addition, ROC curves demonstrated the diagnostic accuracy of markers used. our data demonstrate that microRNA-21 is associated with prediabetic status and exhibits predictive value for early detection of glucose imbalances. These data could provide novel clues for miR-based biomarkers to evaluate diabetes.
Lucia La Sala; Simona Mrakic-Sposta; Elena Tagliabue; Francesco Prattichizzo; Stefano Micheloni; Elena Sangalli; Claudia Specchia; Anna Chiara Uccellatore; Silvia Lupini; Gaia Spinetti; Paola De Candia; Antonio Ceriello. Circulating microRNA-21 is an early predictor of ROS-mediated damage in subjects with high risk of developing diabetes and in drug-naïve T2D. Cardiovascular Diabetology 2019, 18, 1 -12.
AMA StyleLucia La Sala, Simona Mrakic-Sposta, Elena Tagliabue, Francesco Prattichizzo, Stefano Micheloni, Elena Sangalli, Claudia Specchia, Anna Chiara Uccellatore, Silvia Lupini, Gaia Spinetti, Paola De Candia, Antonio Ceriello. Circulating microRNA-21 is an early predictor of ROS-mediated damage in subjects with high risk of developing diabetes and in drug-naïve T2D. Cardiovascular Diabetology. 2019; 18 (1):1-12.
Chicago/Turabian StyleLucia La Sala; Simona Mrakic-Sposta; Elena Tagliabue; Francesco Prattichizzo; Stefano Micheloni; Elena Sangalli; Claudia Specchia; Anna Chiara Uccellatore; Silvia Lupini; Gaia Spinetti; Paola De Candia; Antonio Ceriello. 2019. "Circulating microRNA-21 is an early predictor of ROS-mediated damage in subjects with high risk of developing diabetes and in drug-naïve T2D." Cardiovascular Diabetology 18, no. 1: 1-12.
Purpose: Malignant pleural mesothelioma (MPM) is an aggressive tumor characterized by poor prognosis. Its incidence is steadily increasing due to widespread asbestos exposure. There is still no effective therapy for MPM. Pemetrexed (Pe) is one of the few chemotherapeutic agents approved for advanced-stage disease, although the objective response to the drug is limited. The use of gold nanoparticles (GNPs) as a drug delivery system promises several advantages, including specific targeting of malignant cells, with increased intracellular drug accumulation and reduced systemic toxicity, and, in the case of MPM, direct treatment administration into the pleural space. This study aims at exploring CD146 as a potential MPM cell-specific target for engineered Pe-loaded GNPs and to assess their effectiveness in inhibiting MPM cell line growth. Methods: MPM cell lines and primary cultures obtained by pleural effusions from MPM patients were assayed for CD146 expression by flow cytometry. Internalization by MPM cell lines of fluorescent dye-marked GNPs decorated with a monoclonal anti CD146 coated GNPs (GNP-HC) was proven by confocal microscopy. The effects of anti CD146 coated GNPs loaded with Pe (GNP-HCPe) on MPM cell lines were evaluated by cell cycle (flow cytometry), viability (MTT test), clonogenic capacity (soft agar assay), ROS production (electric paramagnetic resonance), motility (wound healing assay), and apoptosis (flow cytometry). Results: GNP-HC were selectively uptaken by MPM cells within 1 hour. MPM cell lines were blocked in the S cell cycle phase in the presence of GNP-HCPe. Both cell viability and motility were significantly affected by nanoparticle treatment compared to Pe. Apoptotic rate and ROS production were significantly higher in the presence of nanoparticles. Clonogenic capacity was completely inhibited following nanoparticle internalization. Conclusion: GNP-HCPe treatment displays in vitro antineoplastic action and is more effective than Pe alone in inhibiting MPM cell line malignant phenotype. The innovative use of specifically targeted GNPs opens the perspective of local intrapleural administration to avoid normal cell toxicity and enhance chemotherapy efficacy.
Emanuela Cova; Laura Pandolfi; Miriam Colombo; Vanessa Frangipane; Simona Inghilleri; Monica Morosini; Simona Mrakic-Sposta; Sarah Moretti; Manuela Monti; Ymera Pignochino; Silvia Benvenuti; Davide Prosperi; Giulia Stella; Patrizia Morbini; Federica Meloni. Pemetrexed-loaded nanoparticles targeted to malignant pleural mesothelioma cells: an in vitro study. International Journal of Nanomedicine 2019, ume 14, 773 -785.
AMA StyleEmanuela Cova, Laura Pandolfi, Miriam Colombo, Vanessa Frangipane, Simona Inghilleri, Monica Morosini, Simona Mrakic-Sposta, Sarah Moretti, Manuela Monti, Ymera Pignochino, Silvia Benvenuti, Davide Prosperi, Giulia Stella, Patrizia Morbini, Federica Meloni. Pemetrexed-loaded nanoparticles targeted to malignant pleural mesothelioma cells: an in vitro study. International Journal of Nanomedicine. 2019; ume 14 ():773-785.
Chicago/Turabian StyleEmanuela Cova; Laura Pandolfi; Miriam Colombo; Vanessa Frangipane; Simona Inghilleri; Monica Morosini; Simona Mrakic-Sposta; Sarah Moretti; Manuela Monti; Ymera Pignochino; Silvia Benvenuti; Davide Prosperi; Giulia Stella; Patrizia Morbini; Federica Meloni. 2019. "Pemetrexed-loaded nanoparticles targeted to malignant pleural mesothelioma cells: an in vitro study." International Journal of Nanomedicine ume 14, no. : 773-785.
Multidomain instrumental evaluation of post-stroke chronic patients, coupled with standard clinical assessments, has rarely been exploited in the literature. Such an approach may be valuable to provide comprehensive insight regarding patients’ status, as well as orienting the rehabilitation therapies. Therefore, we propose a multidomain analysis including clinically compliant methods as electroencephalography (EEG), electromyography (EMG), kinematics, and clinical scales. The framework of upper-limb robot-assisted rehabilitation is selected as a challenging and promising scenario to test the multi-parameter evaluation, with the aim to assess whether and in which domains modifications may take place. Instrumental recordings and clinical scales were administered before and after a month of intensive robotic therapy of the impaired upper limb, on five post-stroke chronic hemiparetic patients. After therapy, all patients showed clinical improvement and presented pre/post modifications in one or several of the other domains as well. All patients performed the motor task in a smoother way; two of them appeared to change their muscle synergies activation strategies, and most subjects showed variations in their brain activity, both in the ipsi- and contralateral hemispheres. Changes highlighted by the new multiparametric instrumental approach suggest a recovery trend in agreement with clinical scales. In addition, by jointly demonstrating lateralization of brain activations, changes in muscle recruitment and the execution of smoother trajectories, the new approach may help distinguish between true functional recovery and the adoption of suboptimal compensatory strategies. In the light of these premises, the multi-domain approach may allow a finer patient characterization, providing a deeper insight into the mechanisms underlying the relearning procedure and the level (neuro/muscular) at which it occurred, at a relatively low expenditure. The role of this quantitative description in defining a personalized treatment strategy is of great interest and should be addressed in future studies.
Antonella Belfatto; Alessandro Scano; Andrea Chiavenna; Alfonso Mastropietro; Simona Mrakic-Sposta; Simone Pittaccio; Lorenzo Molinari Tosatti; Franco Molteni; Giovanna Rizzo. A Multiparameter Approach to Evaluate Post-Stroke Patients: An Application on Robotic Rehabilitation. Applied Sciences 2018, 8, 2248 .
AMA StyleAntonella Belfatto, Alessandro Scano, Andrea Chiavenna, Alfonso Mastropietro, Simona Mrakic-Sposta, Simone Pittaccio, Lorenzo Molinari Tosatti, Franco Molteni, Giovanna Rizzo. A Multiparameter Approach to Evaluate Post-Stroke Patients: An Application on Robotic Rehabilitation. Applied Sciences. 2018; 8 (11):2248.
Chicago/Turabian StyleAntonella Belfatto; Alessandro Scano; Andrea Chiavenna; Alfonso Mastropietro; Simona Mrakic-Sposta; Simone Pittaccio; Lorenzo Molinari Tosatti; Franco Molteni; Giovanna Rizzo. 2018. "A Multiparameter Approach to Evaluate Post-Stroke Patients: An Application on Robotic Rehabilitation." Applied Sciences 8, no. 11: 2248.
Fronto-temporal dementia (FTD) is the clinical-diagnostic term that is now preferred to describe patients with a range of progressive dementia syndromes associated with focal atrophy of the frontal and anterior temporal cerebral regions. Currently available FTD medications have been used to control behavioral symptoms, even though they are ineffective in some patients, expensive and may induce adverse effects. Alternative therapeutic approaches are worth pursuing, such as non-invasive brain stimulation with transcranial direct current (tDCS). tDCS has been demonstrated to influence neuronal excitability and reported to enhance cognitive performance in dementia. The aim of this study was to investigate whether applying Anodal tDCS (2 mA intensity, 20 min) over the fronto-temporal cortex bilaterally in five consecutive daily sessions would improve cognitive performance and behavior symptoms in FTD patients, also considering the neuromodulatory effect of stimulation on cortical electrical activity measured through EEG. We recruited 13 patients with FTD and we tested the effect of Anodal and Sham (i.e., placebo) tDCS in two separate experimental sessions. In each session, at baseline (T0), after 5 consecutive days (T1), after 1 week (T2), and after 4 weeks (T3) from the end of the treatment, cognitive and behavioral functions were tested. EEG (21 electrodes, 10–20 international system) was recorded for 5 min with eyes closed at the same time points in nine patients. The present findings showed that Anodal tDCS applied bilaterally over the fronto-temporal cortex significantly improves (1) neuropsychiatric symptoms (as measured by the neuropsychiatric inventory, NPI) in FTD patients immediately after tDCS treatment, and (2) simple visual reaction times (sVRTs) up to 1 month after tDCS treatment. These cognitive improvements significantly correlate with the time course of the slow EEG oscillations (delta and theta bands) measured at the same time points. Even though further studies on larger samples are needed, these findings support the effectiveness of Anodal tDCS over the fronto-temporal regions in FTD on attentional processes that might be correlated to a normalized EEG low-frequency pattern.
Roberta Ferrucci; Simona Mrakic-Sposta; Simona Gardini; Fabiana Ruggiero; Maurizio Vergari; Francesca Mameli; Andrea Arighi; Marco Spallazzi; Federica Barocco; Giovanni Michelini; Anna Margherita Pietroboni; Laura Ghezzi; Giorgio Giulio Fumagalli; Giordano D'urso; Paolo Caffarra; Elio Scarpini; Alberto Priori; Sara Marceglia. Behavioral and Neurophysiological Effects of Transcranial Direct Current Stimulation (tDCS) in Fronto-Temporal Dementia. Frontiers in Behavioral Neuroscience 2018, 12, 1 .
AMA StyleRoberta Ferrucci, Simona Mrakic-Sposta, Simona Gardini, Fabiana Ruggiero, Maurizio Vergari, Francesca Mameli, Andrea Arighi, Marco Spallazzi, Federica Barocco, Giovanni Michelini, Anna Margherita Pietroboni, Laura Ghezzi, Giorgio Giulio Fumagalli, Giordano D'urso, Paolo Caffarra, Elio Scarpini, Alberto Priori, Sara Marceglia. Behavioral and Neurophysiological Effects of Transcranial Direct Current Stimulation (tDCS) in Fronto-Temporal Dementia. Frontiers in Behavioral Neuroscience. 2018; 12 ():1.
Chicago/Turabian StyleRoberta Ferrucci; Simona Mrakic-Sposta; Simona Gardini; Fabiana Ruggiero; Maurizio Vergari; Francesca Mameli; Andrea Arighi; Marco Spallazzi; Federica Barocco; Giovanni Michelini; Anna Margherita Pietroboni; Laura Ghezzi; Giorgio Giulio Fumagalli; Giordano D'urso; Paolo Caffarra; Elio Scarpini; Alberto Priori; Sara Marceglia. 2018. "Behavioral and Neurophysiological Effects of Transcranial Direct Current Stimulation (tDCS) in Fronto-Temporal Dementia." Frontiers in Behavioral Neuroscience 12, no. : 1.
The growing elderly population and the increased incidence of mild cognitive impairment (MCI) and Alzheimer’s disease (AD) call for the improvement of the quality and the efficacy of the healthcare and social support services. Exercise and cognitive stimulation have been demonstrated to mitigate cognitive impairment and oxidative stress (OxS) has been recognized as a factor that contributes to the advancement of neurodegenerative diseases. Taking these aspects into account, the impact of a novel virtual reality (VR)-based program combining aerobic exercise and cognitive training has been evaluated in the pilot study proposed here. Ten patients (aged 73.3 ± 5.7 years) with MCI (Mini-Mental State Examination, MMSE: 23.0 ± 3.4) were randomly assigned to either 6 weeks physical and cognitive training (EXP) or control (CTR) group. Evaluations of cognitive profile, by a neuropsychological tests battery, and OxS, by collection of blood and urine samples, were performed before and at the end of the experimental period. The assessment of the patients’ opinions toward the intervention was investigated through questionnaires. EXP group showed a tendency towards improvements in the MMSE, in visual-constructive test and visuo-spatial tests of attention, while CTR worsened. EXP group showed a greater improvement than CTR in the executive test, memory functions and verbal fluency. No statistical significance was obtained when comparing within and between both the groups, probably due to small number of subjects examined, which amplifies the effect of the slight heterogeneity in scores recorded. Despite a greater worsening of Daily Living Activities tests, all participants reported a better performance in real life, thanks to the elicited self-perceived improvement. After training intervention OxS (i.e., reactive oxygen species (ROS) production, oxidative damage of lipids and DNA) decreased resulting in significantly (range p < 0.05–0.001) lower in EXP vs. CTR group. Although not conclusive, the recorded effects in the present study are promising and suggest that this proposal would be a useful tool in support of cognitive training reducing OxS too. However, further studies on larger scale samples of patients are needed.
Simona Mrakic-Sposta; Simona G. Di Santo; Flaminia Franchini; Sara Arlati; Andrea Zangiacomi; Luca Greci; Sarah Moretti; Nithiya Jesuthasan; Mauro Marzorati; Giovanna Rizzo; Marco Sacco; Alessandra Vezzoli. Effects of Combined Physical and Cognitive Virtual Reality-Based Training on Cognitive Impairment and Oxidative Stress in MCI Patients: A Pilot Study. Frontiers in Aging Neuroscience 2018, 10, 282 .
AMA StyleSimona Mrakic-Sposta, Simona G. Di Santo, Flaminia Franchini, Sara Arlati, Andrea Zangiacomi, Luca Greci, Sarah Moretti, Nithiya Jesuthasan, Mauro Marzorati, Giovanna Rizzo, Marco Sacco, Alessandra Vezzoli. Effects of Combined Physical and Cognitive Virtual Reality-Based Training on Cognitive Impairment and Oxidative Stress in MCI Patients: A Pilot Study. Frontiers in Aging Neuroscience. 2018; 10 ():282.
Chicago/Turabian StyleSimona Mrakic-Sposta; Simona G. Di Santo; Flaminia Franchini; Sara Arlati; Andrea Zangiacomi; Luca Greci; Sarah Moretti; Nithiya Jesuthasan; Mauro Marzorati; Giovanna Rizzo; Marco Sacco; Alessandra Vezzoli. 2018. "Effects of Combined Physical and Cognitive Virtual Reality-Based Training on Cognitive Impairment and Oxidative Stress in MCI Patients: A Pilot Study." Frontiers in Aging Neuroscience 10, no. : 282.
This work presents a physical and cognitive training program, based on virtual reality technologies, designed with the aim of preventing the occurrence of symptoms of dementia in elderly with Mild Cognitive Impairment (MCI). The system foresees a physical task to be performed on a cycle-ergometer and two virtual environments for cognitive stimulation. In this paper, results of different validation phases conducted on both healthy and MCI subjects are described. The presented validation path allowed to implement, in parallel, the two current versions of the setup: the former, optimized to assess the efficacy of the intervention in a randomized clinical trial, which will take place in the next future, and the latter, more experimental, which foresees the employment of immersive environments to increase subjects’ engagement and motivation.
Sara Arlati; Luca Greci; Marta Mondellini; Andrea Zangiacomi; Simona G. Di Santo; Flaminia Franchini; Mauro Marzorati; Simona Mrakic-Sposta; Alessandra Vezzoli. A Virtual Reality-Based Physical and Cognitive Training System Aimed at Preventing Symptoms of Dementia. Lecture Notes of the Institute for Computer Sciences, Social Informatics and Telecommunications Engineering 2018, 117 -125.
AMA StyleSara Arlati, Luca Greci, Marta Mondellini, Andrea Zangiacomi, Simona G. Di Santo, Flaminia Franchini, Mauro Marzorati, Simona Mrakic-Sposta, Alessandra Vezzoli. A Virtual Reality-Based Physical and Cognitive Training System Aimed at Preventing Symptoms of Dementia. Lecture Notes of the Institute for Computer Sciences, Social Informatics and Telecommunications Engineering. 2018; ():117-125.
Chicago/Turabian StyleSara Arlati; Luca Greci; Marta Mondellini; Andrea Zangiacomi; Simona G. Di Santo; Flaminia Franchini; Mauro Marzorati; Simona Mrakic-Sposta; Alessandra Vezzoli. 2018. "A Virtual Reality-Based Physical and Cognitive Training System Aimed at Preventing Symptoms of Dementia." Lecture Notes of the Institute for Computer Sciences, Social Informatics and Telecommunications Engineering , no. : 117-125.
Objectives The aim of this study was to evaluate the acute effects of participation in an Ironman distance triathlon competition on arterial function by ultrasound, in relation to cardiac function and body water content. Methods Twenty‐eight male triathletes participating in an Ironman distance competition underwent carotid, femoral, and cardiac ultrasound examinations. Moreover, the presence of extravascular lung water was identified by lung echo B‐lines (echogenic coherent wedge‐shaped signal with a narrow origin from the hyperechoic pleural line) at rest and within 20 minutes of arrival. Results At the end of the competition, athletes showed an increased heart rate (mean ± SD, from 60.2 ± 13.1 to 82.8 ± 15.6 beats/min; P < .0001) and unchanged mean blood pressure (from 93 ± 14 to 91 ± 10 mm Hg; P > .05) in the presence of negligible dehydration (total body water from 48.0 ± 4.0 to 46.5 ± 3.9 kg; P > .05). Cardiac output increased (from 5.5 ± 1.2 to 6.7 ± 2.4 L/min; P < .05) in the presence of an unchanged stroke volume (from 64 ± 14 to 59 ± 16 mL; P > .05) and unchanged left ventricular elastance (from 1.52 ± 0.48 to 1.39 ± 0.48 mm Hg/mL/m2; P > .05). The mean carotid diameter increased (from 7.19 ± 0.65 to 7.61 ± 0.76 mm; P < .05), whereas the mean femoral diameter was unchanged at the end of the competition (from 10.41 ± 0.83 to 10.49 ± 0.82 mm; P > .05). Carotid intima‐media thickness was significantly reduced (from 537 ± 70 to 495 ± 70 μm; P < .05), whereas B‐lines increased significantly after the competition (from 1 [0–4] to 12 [5–23]; P < .0001). Conclusions These data suggest different acute functional adaptation in central arteries with respect to peripheral leg vessels.
Elisabetta Bianchini; Rosa Maria Bruno; Nicole Di Lascio; Alessandra Vezzoli; Simona Mrakic-Sposta; Anca Irina Corciu; Mario Comassi; Lorenza Pratali. Acute Cardiovascular Adaptation to Strenuous Exercise: An Integrative Ultrasound Study. Journal of Ultrasound in Medicine 2018, 38, 463 -470.
AMA StyleElisabetta Bianchini, Rosa Maria Bruno, Nicole Di Lascio, Alessandra Vezzoli, Simona Mrakic-Sposta, Anca Irina Corciu, Mario Comassi, Lorenza Pratali. Acute Cardiovascular Adaptation to Strenuous Exercise: An Integrative Ultrasound Study. Journal of Ultrasound in Medicine. 2018; 38 (2):463-470.
Chicago/Turabian StyleElisabetta Bianchini; Rosa Maria Bruno; Nicole Di Lascio; Alessandra Vezzoli; Simona Mrakic-Sposta; Anca Irina Corciu; Mario Comassi; Lorenza Pratali. 2018. "Acute Cardiovascular Adaptation to Strenuous Exercise: An Integrative Ultrasound Study." Journal of Ultrasound in Medicine 38, no. 2: 463-470.
Antioxidant enzymes play a fundamental role in counteracting oxidative stress induced by high glucose. Although mitochondrial superoxide dismutase (SOD2) is the principal defence against the toxicity of superoxide anions, the mechanism of its inactivation in diabetic subjects is still poorly understood. Recently, microRNA-21 has been associated with diabetes, although its function remains unclear. We sought to explore the mechanism underlying defective SOD2 antioxidant response in HUVECs during exposures to constant high glucose and oscillating glucose (as glucose variability model, GV) and the role of miR-21 in increasing the susceptibility to oxidative stress by disrupting reactive oxygen species (ROS) homeostasis. HUVECs exposed for 1 week to constant high glucose and GV were subjected to quantitative electron paramagnetic resonance for ROS measurements. Superoxide anions, SOD2 protein levels and mitochondrial membrane potential (ΔΨm) were also evaluated. Endogenous miR-21 and its putative ROS-homeostatic target genes (KRIT1, FoxO1, NFE2L2 and SOD2) were tested using mimic-miR-21 and quantified by qPCR. Luciferase assays were performed to test miR-21/3′-UTR-SOD2 binding. We observed upregulation of microRNA-21, overproduction of superoxide anions and total ROS generation, depolarisation of the mitochondrial membrane potential (ΔΨm) and defective SOD2 antioxidant response in HUVECs subjected to constant high glucose and GV exposures. We also found that exogenous mimic-microRNA-21 targeted putative microRNA-21 ROS-homeostatic target genes, e.g., KRIT1, NRF2 and SOD2, which were significantly downregulated. All these effects were reverted by a microRNA-21 inhibitor, which improved SOD2 and KRIT1 expression, reduced the levels of ROS production and ameliorated ΔΨm. Our data demonstrate the association of microRNA-21 with oscillating and high glucose and early mitochondrial dysfunction. We found that microRNA-21 may promote the suppression of homeostatic signalling that normally limits ROS damage. These data provide novel clues about the inhibition of microRNA-21 as a new therapeutic approach to protect against cellular oxidative injury in glucose variability and diabetes.
Lucia La Sala; Simona Mrakic-Sposta; Stefano Micheloni; Francesco Prattichizzo; Antonio Ceriello. Glucose-sensing microRNA-21 disrupts ROS homeostasis and impairs antioxidant responses in cellular glucose variability. Cardiovascular Diabetology 2018, 17, 1 -14.
AMA StyleLucia La Sala, Simona Mrakic-Sposta, Stefano Micheloni, Francesco Prattichizzo, Antonio Ceriello. Glucose-sensing microRNA-21 disrupts ROS homeostasis and impairs antioxidant responses in cellular glucose variability. Cardiovascular Diabetology. 2018; 17 (1):1-14.
Chicago/Turabian StyleLucia La Sala; Simona Mrakic-Sposta; Stefano Micheloni; Francesco Prattichizzo; Antonio Ceriello. 2018. "Glucose-sensing microRNA-21 disrupts ROS homeostasis and impairs antioxidant responses in cellular glucose variability." Cardiovascular Diabetology 17, no. 1: 1-14.
OxS is involved in CD tissue damage and correlates with the degree of duodenal atrophy. These findings suggest the possible role of OxS biomarkers as indicators of CD activity during the clinical follow-up.
Sarah Moretti; Simona Mrakic-Sposta; Leda Roncoroni; Alessandra Vezzoli; Cinzia Dellanoce; Erika Monguzzi; Federica Branchi; Francesca Ferretti; Vincenza Lombardo; Luisa Doneda; Alice Scricciolo; Luca Elli. Oxidative stress as a biomarker for monitoring treated celiac disease. Clinical and Translational Gastroenterology 2018, 9, e157 .
AMA StyleSarah Moretti, Simona Mrakic-Sposta, Leda Roncoroni, Alessandra Vezzoli, Cinzia Dellanoce, Erika Monguzzi, Federica Branchi, Francesca Ferretti, Vincenza Lombardo, Luisa Doneda, Alice Scricciolo, Luca Elli. Oxidative stress as a biomarker for monitoring treated celiac disease. Clinical and Translational Gastroenterology. 2018; 9 (6):e157.
Chicago/Turabian StyleSarah Moretti; Simona Mrakic-Sposta; Leda Roncoroni; Alessandra Vezzoli; Cinzia Dellanoce; Erika Monguzzi; Federica Branchi; Francesca Ferretti; Vincenza Lombardo; Luisa Doneda; Alice Scricciolo; Luca Elli. 2018. "Oxidative stress as a biomarker for monitoring treated celiac disease." Clinical and Translational Gastroenterology 9, no. 6: e157.