Health monitoring sensors that are attached to clothing are a new trend of the times, especially stretchable sensors for human motion measurements or biological markers. However, price, durability, and performance always are major problems to be addressed and three-dimensional (3D) printing combined with conductive flexible materials (thermoplastic polyurethane) can be an optimal solution. Herein, we evaluate the effects of 3D printing-line directions (45°, 90°, 180°) on the sensor performances. Using fused filament fabrication (FDM) technology, the sensors are created with different print styles for specific purposes. We also discuss some main issues of the stretch sensors from Carbon Nanotube/Thermoplastic Polyurethane (CNT/TPU) and FDM. Our sensor achieves outstanding stability (10,000 cycles) and reliability, which are verified through repeated measurements. Its capability is demonstrated in a real application when detecting finger motion by a sensor-integrated into gloves. This paper is expected to bring contribution to the development of flexible conductive materials—based on 3D printing.