After strip mining, soils typically suffer from compaction, low nutrient availability, loss of soil organic carbon, and a compromised soil microbial community. Prairie restorations can improve ecosystem services on former agricultural lands, but prairie restorations on mine lands are relatively under-studied. This study investigated the impact of prairie restoration on mine lands, focusing on the plant community and soil properties. In southeast Ohio, 305 ha within a ~2000 ha area of former mine land was converted to native prairie through herbicide and planting between 1999–2016. Soil and vegetation sampling occurred from 2016–2018. Plant community composition shifted with prairie age, with highest native cover in the oldest prairie areas. Prairie plants were more abundant in older prairies. The oldest prairies had significantly more soil fungal biomass and higher soil microbial biomass. However, many soil properties (e.g., soil nutrients, β-glucosoidase activity, and soil organic carbon), as well as plant species diversity and richness trended higher in prairies, but were not significantly different from baseline cool-season grasslands. Overall, restoration with prairie plant communities slowly shifted soil properties, but mining disturbance was still the most significant driver in controlling soil properties. Prairie restoration on reclaimed mine land was effective in establishing a native plant community, with the associated ecosystem benefits.

Since the passage of legislation in 1977, Appalachian mineland reclamation is typically completed using non-native C₃ grasses and forbs. Alternatively, reclamation with native prairie (C₄) grasses and forbs offers a more ecologically friendly alternative that can contribute to native plant conservation and potentially improve soil properties more quickly than shallower rooted C₃ cool-season grasses. We assessed the establishment of native prairie after reclamation, evaluating three treatments for six years after planting—traditional cool season planting, native prairie planted at light density, and native prairie planted at heavy density. All treatments reached the objectives of reclamation—percentage of ground covered by vegetation—within 2 years after planting. All treatments at all sites, except for one site by treatment combination near a forest, showed an increase in plant species richness and Shannon–Wiener diversity in the first four years of reclamation, a peak around 5 years, and subsequent decrease. Little difference in plant richness and Shannon–Wiener diversity among treatments was observed. However, the two native seed mixes quickly diverged from the traditional mix in terms of community structure and diverged further over time, with both native treatments heading towards a more desirable native prairie grassland state, while the traditional mix remained dominated by non-native cool season grasses. The native treatments also exhibited greater increase in microbial biomass and fungi:bacteria ratio over time compared to the traditional mix. Soil organic carbon increased over time regardless of seed mix treatment. Exchangeable base cations and phosphorus generally decreased over time, as expected, regardless of seed mix treatment, likely due to uptake from established plants. Native grassland species were able to establish despite inclusion of some traditional species in the native mix. Native plant establishment likely resulted in benefits including pollinator resources, bird and wildlife habitat, and increased soil health, and we recommend that native prairie mixes be used directly in reclamation moving forward, as they are able to meet reclamation goals while establishing a successful native prairie plant community.