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Mycobacteriosis affects wild fish and aquaculture worldwide, and alternatives to antibiotics are needed for an effective and environmentally sound control of infectious diseases. Probiotics have shown beneficial effects on fish growth, nutrient metabolism, immune responses, disease prevention and control, and gut microbiota with higher water quality. However, the identification and characterization of the molecules and mechanisms associated with probiotics is a challenge that requires investigation. To address this challenge, herein we used the zebrafish model for the study of the efficacy and mechanisms of probiotic interventions against tuberculosis. First, bacteria from fish gut microbiota were identified with high content of the surface glycotope Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) that has been shown to induce protective immune responses. The results showed that probiotics of selected bacteria with high α-Gal content, namely Aeromonas veronii and Pseudomonas entomophila, were biosafe and effective for the control of Mycobacterium marinum. Protective mechanisms regulating immunity and metabolism activated in response to α-Gal and probiotics with high α-Gal content included modification of gut microbiota composition, B-cell maturation, anti-α-Gal antibodies-mediated control of mycobacteria, induced innate immune responses, beneficial effects on nutrient metabolism and reduced oxidative stress. These results support the potential of probiotics with high α-Gal content for the control of fish mycobacteriosis and suggested the possibility of exploring the development of combined probiotic treatments alone and in combination with α-Gal for the control of infectious diseases.
Iván Pacheco; Sandra Díaz-Sánchez; Marinela Contreras; Margarita Villar; Alejandro Cabezas-Cruz; Christian Gortázar; José de la Fuente. Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis. Pharmaceuticals 2021, 14, 635 .
AMA StyleIván Pacheco, Sandra Díaz-Sánchez, Marinela Contreras, Margarita Villar, Alejandro Cabezas-Cruz, Christian Gortázar, José de la Fuente. Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis. Pharmaceuticals. 2021; 14 (7):635.
Chicago/Turabian StyleIván Pacheco; Sandra Díaz-Sánchez; Marinela Contreras; Margarita Villar; Alejandro Cabezas-Cruz; Christian Gortázar; José de la Fuente. 2021. "Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis." Pharmaceuticals 14, no. 7: 635.
Coronavirus-like organisms have been previously identified in Arthropod ectoparasites (such as ticks and unfed cat flea). Yet, the question regarding the possible role of these arthropods as SARS-CoV-2 passive/biological transmission vectors is still poorly explored. In this study, we performed in silico structural and binding energy calculations to assess the risks associated with possible ectoparasite transmission. We found sufficient similarity between ectoparasite ACE and human ACE2 protein sequences to build good quality 3D-models of the SARS-CoV-2 Spike:ACE complex to assess the impacts of ectoparasite mutations on complex stability. For several species (e.g., water flea, deer tick, body louse), our analyses showed no significant destabilisation of the SARS-CoV-2 Spike:ACE complex, suggesting these species would bind the viral Spike protein. Our structural analyses also provide structural rationale for interactions between the viral Spike and the ectoparasite ACE proteins. Although we do not have experimental evidence of infection in these ectoparasites, the predicted stability of the complex suggests this is possible, raising concerns of a possible role in passive transmission of the virus to their human hosts.
Su Lam; Paul Ashford; Sandra Díaz-Sánchez; Margarita Villar; Christian Gortázar; José de la Fuente; Christine Orengo. Arthropod Ectoparasites Have Potential to Bind SARS-CoV-2 via ACE. Viruses 2021, 13, 708 .
AMA StyleSu Lam, Paul Ashford, Sandra Díaz-Sánchez, Margarita Villar, Christian Gortázar, José de la Fuente, Christine Orengo. Arthropod Ectoparasites Have Potential to Bind SARS-CoV-2 via ACE. Viruses. 2021; 13 (4):708.
Chicago/Turabian StyleSu Lam; Paul Ashford; Sandra Díaz-Sánchez; Margarita Villar; Christian Gortázar; José de la Fuente; Christine Orengo. 2021. "Arthropod Ectoparasites Have Potential to Bind SARS-CoV-2 via ACE." Viruses 13, no. 4: 708.
Ticks and tick-borne diseases (TBDs) represent a burden for human and animal health worldwide. Currently, vaccines constitute the safest and most effective approach to control ticks and TBDs. Subolesin (SUB) has been identified as a vaccine antigen for the control of tick infestations and pathogen infection and transmission. The characterization of the molecular function of SUB and the identification of tick proteins interacting with SUB may provide the basis for the discovery of novel antigens and for the rational design of novel anti-tick vaccines. In the present study, we used the yeast two-hybrid system (Y2H) as an unbiased approach to identify tick SUB-interacting proteins in an Ixodes ricinus cDNA library, and studied the possible role of SUB as a chromatin remodeler through direct interaction with histones. The Y2H screening identified Importin-α as a potential SUB-interacting protein, which was confirmed in vitro in a protein pull-down assay. The sub gene expression levels in tick midgut and fat body were significantly higher in unfed than fed female ticks, however, the importin-α expression levels did not vary between unfed and fed ticks but tended to be higher in the ovary when compared to those in other organs. The effect of importin-α RNAi was characterized in I. ricinus under artificial feeding conditions. Both sub and importin-α gene knockdown was observed in all tick tissues and, while tick weight was significantly lower in sub RNAi-treated ticks than in controls, importin-α RNAi did not affect tick feeding or oviposition, suggesting that SUB is able to exert its function in the absence of Importin-α. Furthermore, SUB was shown to physically interact with histone 4, which was corroborated by protein pull-down and western blot analysis. These results confirm that by interacting with numerous tick proteins, SUB is a key cofactor of the tick interactome and regulome. Further studies are needed to elucidate the nature of the SUB-Importin-α interaction and the biological processes and functional implications that this interaction may have.
Sara Artigas-Jerónimo; Margarita Villar; Alejandro Cabezas-Cruz; Grégory Caignard; Damien Vitour; Jennifer Richardson; Sandrine Lacour; Houssam Attoui; Lesley Bell-Sakyi; Eleonore Allain; Ard Nijhof; Nina Militzer; Sophia Pinecki Socias; José de la Fuente. Tick Importin-α Is Implicated in the Interactome and Regulome of the Cofactor Subolesin. Pathogens 2021, 10, 457 .
AMA StyleSara Artigas-Jerónimo, Margarita Villar, Alejandro Cabezas-Cruz, Grégory Caignard, Damien Vitour, Jennifer Richardson, Sandrine Lacour, Houssam Attoui, Lesley Bell-Sakyi, Eleonore Allain, Ard Nijhof, Nina Militzer, Sophia Pinecki Socias, José de la Fuente. Tick Importin-α Is Implicated in the Interactome and Regulome of the Cofactor Subolesin. Pathogens. 2021; 10 (4):457.
Chicago/Turabian StyleSara Artigas-Jerónimo; Margarita Villar; Alejandro Cabezas-Cruz; Grégory Caignard; Damien Vitour; Jennifer Richardson; Sandrine Lacour; Houssam Attoui; Lesley Bell-Sakyi; Eleonore Allain; Ard Nijhof; Nina Militzer; Sophia Pinecki Socias; José de la Fuente. 2021. "Tick Importin-α Is Implicated in the Interactome and Regulome of the Cofactor Subolesin." Pathogens 10, no. 4: 457.
Tick vaccination is an environmentally friendly alternative for tick control, pathogen infection, and transmission. Tick vaccine protection is sometimes incomplete, which may be due to problems in the stability, conformation, and activity of antibodies. This might be related to oxidative stress, but more studies are needed about the possible relationships between oxidative stress and immune function. The objective of this study was to evaluate and compare various serum biomarkers of antioxidant response and oxidative damage in cattle vaccinated with two recombinant antigens, the chimera of Subolesin- BM95 (homologue antigen of BM86)-MSP1a and BM86, and a control consisting in the adjuvant of the vaccines. Cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of the plasma (FRAP), trolox equivalent antioxidant capacity (TEAC), total thiol concentrations, and uric acid were evaluated in serum to determine the antioxidant response. To evaluate oxidative status, ferrous oxidation-xylenol orange (FOX), total oxidant status (TOS), advanced oxidation protein products (AOPP) and hydrogen peroxide (H2O2) concentrations in serum were determined. In addition, correlations between biomarkers of oxidative stress and antibody titers were evaluated. A significant decrease in all antioxidant biomarkers, with exception of thiol, and also a decrease in the oxidant markers TOS, AOPP and H2O2 was observed in cattle vaccinated with BM86, that also showed the highest antibody titers response whereas no significant differences in any of the biomarkers were detected in the Subolesin-Bm95-MSP1a and control groups. In addition, the dynamics of Cuprac and H2O2 with time showed significant differences between the groups. Although this is a pilot study and the results should be interpreted with caution and corroborated by studies involving a large number of animals, our results indicate that, in our experimental conditions, those vaccines able to induce a lower oxidative stress produce a higher concentration of antigen-specific antibodies. Overall, the results of the study provided information on the behavior of different biomarkers related to antioxidant defense, and the oxidative damage in cattle in response to vaccination.
Marinela Contreras; Camila Peres Rubio; José De La Fuente; Margarita Villar; Octavio Merino; Juan Mosqueda Gualito; José Joaquín Cerón. Changes in Serum Biomarkers of Oxidative Stress in Cattle Vaccinated with Tick Recombinant Antigens: A Pilot Study. Vaccines 2020, 9, 5 .
AMA StyleMarinela Contreras, Camila Peres Rubio, José De La Fuente, Margarita Villar, Octavio Merino, Juan Mosqueda Gualito, José Joaquín Cerón. Changes in Serum Biomarkers of Oxidative Stress in Cattle Vaccinated with Tick Recombinant Antigens: A Pilot Study. Vaccines. 2020; 9 (1):5.
Chicago/Turabian StyleMarinela Contreras; Camila Peres Rubio; José De La Fuente; Margarita Villar; Octavio Merino; Juan Mosqueda Gualito; José Joaquín Cerón. 2020. "Changes in Serum Biomarkers of Oxidative Stress in Cattle Vaccinated with Tick Recombinant Antigens: A Pilot Study." Vaccines 9, no. 1: 5.
The alpha-Gal syndrome (AGS) is associated with tick bites that can induce in humans high levels of IgE antibodies against the carbohydrate Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) present in glycoproteins and glycolipids from tick saliva that mediate primarily delayed anaphylaxis to mammalian meat consumption. It has been proposed that humans evolved by losing the capacity to synthesize α-Gal to increase the protective immune response against pathogens with this modification on their surface. This evolutionary adaptation suggested the possibility of developing vaccines and other interventions to induce the anti-α-Gal IgM/IgG protective response against pathogen infection and multiplication. However, the protective effect of the anti-α-Gal immune response for the control of tuberculosis caused by Mycobacterium spp. has not been explored. To address the possibility of using vaccination with α-Gal for the control of tuberculosis, in this study, we used the zebrafish-Mycobacterium marinum model. The results showed that vaccination with α-Gal protected against mycobacteriosis in the zebrafish model of tuberculosis and provided evidence on the protective mechanisms in response to vaccination with α-Gal. These mechanisms included B-cell maturation, antibody-mediated opsonization of mycobacteria, Fc-receptor (FcR)-mediated phagocytosis, macrophage response, interference with the α-Gal antagonistic effect of the toll-like receptor 2 (TLR2)/nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB)-mediated immune response, and upregulation of pro-inflammatory cytokines. These results provided additional evidence supporting the role of the α-Gal-induced immune response in the control of infections caused by pathogens with this modification on their surface and the possibility of using this approach for the control of multiple infectious diseases.
Iván Pacheco; Marinela Contreras; Margarita Villar; María Angeles Risalde; Pilar Alberdi; Alejandro Cabezas-Cruz; Christian Gortázar; José De La Fuente. Vaccination with Alpha-Gal Protects Against Mycobacterial Infection in the Zebrafish Model of Tuberculosis. Vaccines 2020, 8, 195 .
AMA StyleIván Pacheco, Marinela Contreras, Margarita Villar, María Angeles Risalde, Pilar Alberdi, Alejandro Cabezas-Cruz, Christian Gortázar, José De La Fuente. Vaccination with Alpha-Gal Protects Against Mycobacterial Infection in the Zebrafish Model of Tuberculosis. Vaccines. 2020; 8 (2):195.
Chicago/Turabian StyleIván Pacheco; Marinela Contreras; Margarita Villar; María Angeles Risalde; Pilar Alberdi; Alejandro Cabezas-Cruz; Christian Gortázar; José De La Fuente. 2020. "Vaccination with Alpha-Gal Protects Against Mycobacterial Infection in the Zebrafish Model of Tuberculosis." Vaccines 8, no. 2: 195.
Ticks are obligate hematophagous arthropods and vectors of pathogens affecting human and animal health worldwide. Cement is a complex protein polymerization substance secreted by ticks with antimicrobial properties and a possible role in host attachment, sealing the feeding lesion, facilitating feeding and pathogen transmission, and protection from host immune and inflammatory responses. The biochemical properties of tick cement during feeding have not been fully characterized. In this study, we characterized the proteome of Rhipicephalus microplus salivary glands (sialome) and cement (cementome) together with their physicochemical properties at different adult female parasitic stages. The results showed the combination of tick and host derived proteins and other biomolecules such as α-Gal in cement composition, which varied during the feeding process. We propose that these compounds may synergize in cement formation, solidification and maintenance to facilitate attachment, feeding, interference with host immune response and detachment. These results advanced our knowledge of the complex tick cement composition and suggested that tick and host derived compounds modulate cement properties throughout tick feeding.
Margarita Villar; Iván Pacheco; Octavio Merino; Marinela Contreras; Lourdes Mateos-Hernández; Eduardo Prado; Dina Karen Barros-Picanço; José Francisco Lima-Barbero; Sara Artigas-Jerónimo; Pilar Alberdi; Isabel G. Fernández De Mera; Agustín Estrada-Peña; Alejandro Cabezas-Cruz; José De La Fuente. Tick and Host Derived Compounds Detected in the Cement Complex Substance. Biomolecules 2020, 10, 555 .
AMA StyleMargarita Villar, Iván Pacheco, Octavio Merino, Marinela Contreras, Lourdes Mateos-Hernández, Eduardo Prado, Dina Karen Barros-Picanço, José Francisco Lima-Barbero, Sara Artigas-Jerónimo, Pilar Alberdi, Isabel G. Fernández De Mera, Agustín Estrada-Peña, Alejandro Cabezas-Cruz, José De La Fuente. Tick and Host Derived Compounds Detected in the Cement Complex Substance. Biomolecules. 2020; 10 (4):555.
Chicago/Turabian StyleMargarita Villar; Iván Pacheco; Octavio Merino; Marinela Contreras; Lourdes Mateos-Hernández; Eduardo Prado; Dina Karen Barros-Picanço; José Francisco Lima-Barbero; Sara Artigas-Jerónimo; Pilar Alberdi; Isabel G. Fernández De Mera; Agustín Estrada-Peña; Alejandro Cabezas-Cruz; José De La Fuente. 2020. "Tick and Host Derived Compounds Detected in the Cement Complex Substance." Biomolecules 10, no. 4: 555.
The negative impact of ticks and tick-borne diseases on animals and human health is driving research to discover novel targets affecting both vectors and pathogens. The salivary glands are involved in feeding and pathogen transmission, thus are considered as a compelling target to focus research. In this study, proteomics approach was used to characterize Rhipicephalus bursa sialoproteome in response to Babesia ovis infection and blood feeding. Two potential tick protective antigens were identified and its influence in tick biological parameters and pathogen infection was evaluated. Results demonstrate that the R. bursa sialoproteome is highly affected by feeding but infection is well tolerated by tick cells. The combination of both stimuli shifts the previous scenario and a more evident pathogen manipulation can be suggested. Knockdown of ub2n led to a significative increase of infection in tick salivary glands but a brusque decrease in the progeny, revealing its importance in the cellular response to pathogen infection, which is worth pursuing in future studies. Additionally, an impact in the recovery rate of adults (62%), the egg production efficiency (45.75%), and the hatching rate (88.57 %) was detected. Building knowledge on vector and/or pathogen interplay bridges the identification of protective antigens and the development of novel control strategies.
Joana Couto; Margarita Villar; Lourdes Mateos-Hernández; Joana Ferrolho; Gustavo S. Sanches; Ana Sofia Santos; Maria Margarida Santos-Silva; João Nobre; Olga Moreira; Sandra Antunes; José De La Fuente; Ana Domingos. Quantitative Proteomics Identifies Metabolic Pathways Affected by Babesia Infection and Blood Feeding in the Sialoproteome of the Vector Rhipicephalus bursa. Vaccines 2020, 8, 91 .
AMA StyleJoana Couto, Margarita Villar, Lourdes Mateos-Hernández, Joana Ferrolho, Gustavo S. Sanches, Ana Sofia Santos, Maria Margarida Santos-Silva, João Nobre, Olga Moreira, Sandra Antunes, José De La Fuente, Ana Domingos. Quantitative Proteomics Identifies Metabolic Pathways Affected by Babesia Infection and Blood Feeding in the Sialoproteome of the Vector Rhipicephalus bursa. Vaccines. 2020; 8 (1):91.
Chicago/Turabian StyleJoana Couto; Margarita Villar; Lourdes Mateos-Hernández; Joana Ferrolho; Gustavo S. Sanches; Ana Sofia Santos; Maria Margarida Santos-Silva; João Nobre; Olga Moreira; Sandra Antunes; José De La Fuente; Ana Domingos. 2020. "Quantitative Proteomics Identifies Metabolic Pathways Affected by Babesia Infection and Blood Feeding in the Sialoproteome of the Vector Rhipicephalus bursa." Vaccines 8, no. 1: 91.
The main objective of this study was to propose a novel methodology to approach challenges in molecular biology. Akirin/Subolesin (AKR/SUB) are vaccine protective antigens and are a model for the study of the interactome due to its conserved function in the regulation of different biological processes such as immunity and development throughout the metazoan. Herein, three visual artists and a music professor collaborated with scientists for the functional characterization of the AKR2 interactome in the regulation of the NF-κB pathway in human placenta cells. The results served as a methodological proof-of-concept to advance this research area. The results showed new perspectives on unexplored characteristics of AKR2 with functional implications. These results included protein dimerization, the physical interactions with different proteins simultaneously to regulate various biological processes defined by cell type-specific AKR–protein interactions, and how these interactions positively or negatively regulate the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway in a biological context-dependent manner. These results suggested that AKR2-interacting proteins might constitute suitable secondary transcription factors for cell- and stimulus-specific regulation of NF-κB. Musical perspective supported AKR/SUB evolutionary conservation in different species and provided new mechanistic insights into the AKR2 interactome. The combined scientific and artistic perspectives resulted in a multidisciplinary approach, advancing our knowledge on AKR/SUB interactome, and provided new insights into the function of AKR2–protein interactions in the regulation of the NF-κB pathway. Additionally, herein we proposed an algorithm for quantum vaccinomics by focusing on the model proteins AKR/SUB.
Sara Artigas-Jerónimo; Juan J. Pastor Comín; Margarita Villar; Marinela Contreras; Pilar Alberdi; Israel León Viera; Leandro Soto; Raúl Cordero; James J. Valdés; Alejandro Cabezas-Cruz; Agustín Estrada-Peña; José De La Fuente. A Novel Combined Scientific and Artistic Approach for the Advanced Characterization of Interactomes: The Akirin/Subolesin Model. Vaccines 2020, 8, 77 .
AMA StyleSara Artigas-Jerónimo, Juan J. Pastor Comín, Margarita Villar, Marinela Contreras, Pilar Alberdi, Israel León Viera, Leandro Soto, Raúl Cordero, James J. Valdés, Alejandro Cabezas-Cruz, Agustín Estrada-Peña, José De La Fuente. A Novel Combined Scientific and Artistic Approach for the Advanced Characterization of Interactomes: The Akirin/Subolesin Model. Vaccines. 2020; 8 (1):77.
Chicago/Turabian StyleSara Artigas-Jerónimo; Juan J. Pastor Comín; Margarita Villar; Marinela Contreras; Pilar Alberdi; Israel León Viera; Leandro Soto; Raúl Cordero; James J. Valdés; Alejandro Cabezas-Cruz; Agustín Estrada-Peña; José De La Fuente. 2020. "A Novel Combined Scientific and Artistic Approach for the Advanced Characterization of Interactomes: The Akirin/Subolesin Model." Vaccines 8, no. 1: 77.
Infestation with the salmon louse Lepeophtheirus salmonis (Copepoda, Caligidae) affects Atlantic salmon (Salmo salar L.) production in European aquaculture. Furthermore, high levels of salmon lice in farms significantly increase challenge pressure against wild salmon populations. Currently, available control methods for salmon louse have limitations, and vaccination appears as an attractive, environmentally sound strategy. In this study, we addressed one of the main limitations for vaccine development, the identification of candidate protective antigens. Based on recent advances in tick vaccine research, herein, we targeted the salmon louse midgut function and blood digestion for the identification of candidate target proteins for the control of ectoparasite infestations. The results of this translational approach resulted in the identification and subsequent evaluation of the new candidate protective antigens, putative Toll-like receptor 6 (P30), and potassium chloride, and amino acid transporter (P33). Vaccination with these antigens provided protection in Atlantic salmon by reducing adult female (P33) or chalimus II (P30) sea lice infestations. These results support the development of vaccines for the control of sea lice infestations.
Marinela Contreras; Marius Karlsen; Margarita Villar; Rolf Hetlelid Olsen; Lisa Marie Leknes; Anette Furevik; Karine Lindmo Yttredal; Haitham Tartor; Soren Grove; Pilar Alberdi; Bjorn Brudeseth; José De La Fuente. Vaccination with Ectoparasite Proteins Involved in Midgut Function and Blood Digestion Reduces Salmon Louse Infestations. Vaccines 2020, 8, 32 .
AMA StyleMarinela Contreras, Marius Karlsen, Margarita Villar, Rolf Hetlelid Olsen, Lisa Marie Leknes, Anette Furevik, Karine Lindmo Yttredal, Haitham Tartor, Soren Grove, Pilar Alberdi, Bjorn Brudeseth, José De La Fuente. Vaccination with Ectoparasite Proteins Involved in Midgut Function and Blood Digestion Reduces Salmon Louse Infestations. Vaccines. 2020; 8 (1):32.
Chicago/Turabian StyleMarinela Contreras; Marius Karlsen; Margarita Villar; Rolf Hetlelid Olsen; Lisa Marie Leknes; Anette Furevik; Karine Lindmo Yttredal; Haitham Tartor; Soren Grove; Pilar Alberdi; Bjorn Brudeseth; José De La Fuente. 2020. "Vaccination with Ectoparasite Proteins Involved in Midgut Function and Blood Digestion Reduces Salmon Louse Infestations." Vaccines 8, no. 1: 32.
The poultry red mite (PRM), Dermanyssus gallinae, is a hematophagous ectoparasite considered as the major pest in the egg-laying industry. Its pesticide-based control is only partially successful and requires the development of new control interventions such as vaccines. In this study, we follow a vaccinology approach to identify PRM candidate protective antigens. Based on proteomic data from fed and unfed nymph and adult mites, we selected a novel PRM protein, calumenin (Deg-CALU), which is tested as a vaccine candidate on an on-hen trial. Rhipicephalus microplus Subolesin (Rhm-SUB) was chosen as a positive control. Deg-CALU and Rhm-SUB reduced the mite oviposition by 35 and 44%, respectively. These results support Deg-CALU and Rhm-SUB as candidate protective antigens for the PRM control.
José Francisco Lima-Barbero; Marinela Contreras; Lourdes Mateos-Hernández; Francisco Manuel Mata-Lorenzo; Roxana Triguero-Ocaña; Olivier Sparagano; Robert D. Finn; Christina Strube; Daniel R.G. Price; Francesca Nunn; Kathryn Bartley; Ursula Höfle; Mariana Boadella; Alasdair J. Nisbet; José De La Fuente; Margarita Villar. A Vaccinology Approach to the Identification and Characterization of Dermanyssus gallinae Candidate Protective Antigens for the Control of Poultry Red Mite Infestations. Vaccines 2019, 7, 190 .
AMA StyleJosé Francisco Lima-Barbero, Marinela Contreras, Lourdes Mateos-Hernández, Francisco Manuel Mata-Lorenzo, Roxana Triguero-Ocaña, Olivier Sparagano, Robert D. Finn, Christina Strube, Daniel R.G. Price, Francesca Nunn, Kathryn Bartley, Ursula Höfle, Mariana Boadella, Alasdair J. Nisbet, José De La Fuente, Margarita Villar. A Vaccinology Approach to the Identification and Characterization of Dermanyssus gallinae Candidate Protective Antigens for the Control of Poultry Red Mite Infestations. Vaccines. 2019; 7 (4):190.
Chicago/Turabian StyleJosé Francisco Lima-Barbero; Marinela Contreras; Lourdes Mateos-Hernández; Francisco Manuel Mata-Lorenzo; Roxana Triguero-Ocaña; Olivier Sparagano; Robert D. Finn; Christina Strube; Daniel R.G. Price; Francesca Nunn; Kathryn Bartley; Ursula Höfle; Mariana Boadella; Alasdair J. Nisbet; José De La Fuente; Margarita Villar. 2019. "A Vaccinology Approach to the Identification and Characterization of Dermanyssus gallinae Candidate Protective Antigens for the Control of Poultry Red Mite Infestations." Vaccines 7, no. 4: 190.
The poultry red mite (PRM), Dermanyssus gallinae, is a hematophagous ectoparasite of birds with worldwide distribution that causes economic losses in the egg-production sector of the poultry industry. Traditional control methods, mainly based on acaricides, have been only partially successful, and new vaccine-based interventions are required for the control of PRM. Vaccination with insect Akirin (AKR) and its homolog in ticks, Subolesin (SUB), have shown protective efficacy for the control of ectoparasite infestations and pathogen infection/transmission. The aim of this study was the identification of the akr gene from D. gallinae (Deg-akr), the production of the recombinant Deg-AKR protein, and evaluation of its efficacy as a vaccine candidate for the control of PRM. The anti-Deg-AKR serum IgY antibodies in hen sera and egg yolk were higher in vaccinated than control animals throughout the experiment. The results demonstrated the efficacy of the vaccination with Deg-AKR for the control of PRM by reducing mite oviposition by 42% following feeding on vaccinated hens. A negative correlation between the levels of serum anti-Deg-AKR IgY and mite oviposition was obtained. These results support Deg-AKR as a candidate protective antigen for the control of PRM population growth.
Jose Francisco Lima-Barbero; Marinela Contreras; Kathryn Bartley; Daniel R. G. Price; Francesca Nunn; Marta Sanchez-Sanchez; Eduardo Prado; Ursula Höfle; Margarita Villar; Alasdair J. Nisbet; José De La Fuente. Reduction in Oviposition of Poultry Red Mite (Dermanyssus gallinae) in Hens Vaccinated with Recombinant Akirin. Vaccines 2019, 7, 121 .
AMA StyleJose Francisco Lima-Barbero, Marinela Contreras, Kathryn Bartley, Daniel R. G. Price, Francesca Nunn, Marta Sanchez-Sanchez, Eduardo Prado, Ursula Höfle, Margarita Villar, Alasdair J. Nisbet, José De La Fuente. Reduction in Oviposition of Poultry Red Mite (Dermanyssus gallinae) in Hens Vaccinated with Recombinant Akirin. Vaccines. 2019; 7 (3):121.
Chicago/Turabian StyleJose Francisco Lima-Barbero; Marinela Contreras; Kathryn Bartley; Daniel R. G. Price; Francesca Nunn; Marta Sanchez-Sanchez; Eduardo Prado; Ursula Höfle; Margarita Villar; Alasdair J. Nisbet; José De La Fuente. 2019. "Reduction in Oviposition of Poultry Red Mite (Dermanyssus gallinae) in Hens Vaccinated with Recombinant Akirin." Vaccines 7, no. 3: 121.
Mammalian sperm must undergo a set of structural and functional changes collectively termed as capacitation to ensure a successful oocyte fertilization. However, capacitation can be compromised by cryopreservation procedures, which alter the proteome and longevity of sperm. To date, how the protein changes induced by cryopreservation could affect the acquisition of sperm fertilizing potential remains unexplored. The present study investigated the protein profile of ram sperm during in vitro capacitation before and after cryopreservation to elucidate the impact of cryopreservation on sperm capacitation at a molecular level. Fresh and cryopreserved ram sperm were incubated under capacitating (CAP) and non-capacitating (NC) conditions for 240 min. The sperm proteome of these four treatments was analyzed and compared at different incubation times using reverse phase liquid chromatography coupled to mass spectrometry (RP-LC-MS/MS). The comparison between fresh and cryopreserved sperm suggested that cryopreservation facilitated an apoptosis-stress response and redox process, while the comparison between sperm incubated in CAP and NC conditions showed that capacitation increased those biological processes associated with signaling, metabolism, motility, and reproductive processes. In addition, 14 proteins related to mitochondrial activity, sperm motility, oocyte recognition, signaling, spermatogenesis, and the apoptosis-stress response underwent significant changes in abundance over time when fresh and cryopreserved sperm incubated in CAP and NC conditions were compared. Our results indicate that disturbances in a ram sperm proteome after cryopreservation may alter the quality of sperm and its specific machinery to sustain capacitation under in vitro conditions.
Patricia Peris-Frau; Alicia Martín-Maestro; María Iniesta-Cuerda; Irene Sánchez-Ajofrín; Lourdes Mateos-Hernández; J. Julián Garde; Margarita Villar; Ana Josefa Soler. Freezing–Thawing Procedures Remodel the Proteome of Ram Sperm before and after In Vitro Capacitation. International Journal of Molecular Sciences 2019, 20, 4596 .
AMA StylePatricia Peris-Frau, Alicia Martín-Maestro, María Iniesta-Cuerda, Irene Sánchez-Ajofrín, Lourdes Mateos-Hernández, J. Julián Garde, Margarita Villar, Ana Josefa Soler. Freezing–Thawing Procedures Remodel the Proteome of Ram Sperm before and after In Vitro Capacitation. International Journal of Molecular Sciences. 2019; 20 (18):4596.
Chicago/Turabian StylePatricia Peris-Frau; Alicia Martín-Maestro; María Iniesta-Cuerda; Irene Sánchez-Ajofrín; Lourdes Mateos-Hernández; J. Julián Garde; Margarita Villar; Ana Josefa Soler. 2019. "Freezing–Thawing Procedures Remodel the Proteome of Ram Sperm before and after In Vitro Capacitation." International Journal of Molecular Sciences 20, no. 18: 4596.
Due to the functional inactivation of the gene encoding for the enzyme that is involved in the oligosaccharide galactose-α-1,3-galactose (α-Gal) synthesis, humans and Old-World primates are able to produce a large amount of antibodies against the glycan epitope. Apart from being involved in the hyperacute organ rejection in humans, anti-α-Gal antibodies have shown a protective effect against some pathogenic agents and an implication in the recently recognized tick-induced mammalian meat allergy. Conversely, non-primate mammals, including dogs, have the ability to synthetize α-Gal and, thus, their immune system is not expected to naturally generate the antibodies toward this self-antigen molecule. However, in the current study, we detected specific IgG, IgM, and IgE antibodies to α-Gal in sera of clinically healthy dogs by an indirect enzyme-linked immunosorbent assay (ELISA) for the first time. Furthermore, in a tick infestation experiment, we showed that bites of Ixodes ricinus induce the immune response to α-Gal in dogs and that the resulting antibodies (IgM) might be protective against Anaplasma phagocytophilum. These findings may help lead to a better understanding of the underlying mechanisms involved in mammalian meat allergy and tick-host-pathogen interactions, but they also open up the question about the possibility that dogs could develop an allergy to mammalian meat after tick bites, similar to that in humans.
Adnan Hodžić; Lourdes Mateos-Hernández; Michael Leschnik; Pilar Alberdi; Ryan O. M. Rego; Marinela Contreras; Margarita Villar; José De La Fuente; Alejandro Cabezas-Cruz; Georg Gerhard Duscher. Tick Bites Induce Anti-α-Gal Antibodies in Dogs. Vaccines 2019, 7, 114 .
AMA StyleAdnan Hodžić, Lourdes Mateos-Hernández, Michael Leschnik, Pilar Alberdi, Ryan O. M. Rego, Marinela Contreras, Margarita Villar, José De La Fuente, Alejandro Cabezas-Cruz, Georg Gerhard Duscher. Tick Bites Induce Anti-α-Gal Antibodies in Dogs. Vaccines. 2019; 7 (3):114.
Chicago/Turabian StyleAdnan Hodžić; Lourdes Mateos-Hernández; Michael Leschnik; Pilar Alberdi; Ryan O. M. Rego; Marinela Contreras; Margarita Villar; José De La Fuente; Alejandro Cabezas-Cruz; Georg Gerhard Duscher. 2019. "Tick Bites Induce Anti-α-Gal Antibodies in Dogs." Vaccines 7, no. 3: 114.
Aerobic organisms evolved conserved mechanisms controlling the generation of reactive oxygen species (ROS) to maintain redox homeostasis signaling and modulate signal transduction, gene expression and cellular functional responses under physiological conditions. The production of ROS by mitochondria is essential in the oxidative stress associated with different pathologies and in response to pathogen infection. Anaplasma phagocytophilum is an intracellular pathogen transmitted by Ixodes scapularis ticks and causing human granulocytic anaplasmosis. Bacteria multiply in vertebrate neutrophils and infect first tick midgut cells and subsequently hemocytes and salivary glands from where transmission occurs. Previous results demonstrated that A. phagocytophilum does not induce the production of ROS as part of its survival strategy in human neutrophils. However, little is known about the role of ROS during pathogen infection in ticks. In this study, the role of tick oxidative stress during A. phagocytophilum infection was characterized through the function of different pathways involved in ROS production. The results showed that tick cells increase mitochondrial ROS production to limit A. phagocytophilum infection, while pathogen inhibits alternative ROS production pathways and apoptosis to preserve cell fitness and facilitate infection. The inhibition of NADPH oxidase-mediated ROS production by pathogen infection appears to occur in both neutrophils and tick cells, thus supporting that A. phagocytophilum uses common mechanisms for infection of ticks and vertebrate hosts. However, differences in ROS response to A. phagocytophilum infection between human and tick cells may reflect host-specific cell tropism that evolved during pathogen life cycle.
Pilar Alberdi; Alejandro Cabezas-Cruz; Pedro Espinosa Prados; Margarita Villar Rayo; Sara Artigas Jerónimo; José De La Fuente. The redox metabolic pathways function to limit Anaplasma phagocytophilum infection and multiplication while preserving fitness in tick vector cells. Scientific Reports 2019, 9, 1 -15.
AMA StylePilar Alberdi, Alejandro Cabezas-Cruz, Pedro Espinosa Prados, Margarita Villar Rayo, Sara Artigas Jerónimo, José De La Fuente. The redox metabolic pathways function to limit Anaplasma phagocytophilum infection and multiplication while preserving fitness in tick vector cells. Scientific Reports. 2019; 9 (1):1-15.
Chicago/Turabian StylePilar Alberdi; Alejandro Cabezas-Cruz; Pedro Espinosa Prados; Margarita Villar Rayo; Sara Artigas Jerónimo; José De La Fuente. 2019. "The redox metabolic pathways function to limit Anaplasma phagocytophilum infection and multiplication while preserving fitness in tick vector cells." Scientific Reports 9, no. 1: 1-15.
Blood-feeding ectoparasites constitute a growing burden for human and animal health, and animal production worldwide. In particular, mites (Acari: Gamasida) of the genera Dermanyssus (Dermanyssidae) and Ornithonyssus (Macronyssidae) infest birds and cause gamasoidosis in humans. The tropical fowl mite, Ornithonyssus bursa, is commonly found in tropical and subtropical countries but rarely reported in Europe. In this research we characterized the first two cases in Spain of clinical gamasoidosis diagnosed in patients infested with O. bursa, and investigated the IgE, IgM and IgG antibody response to mite proteins and the carbohydrate Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) involved in the tick-bite associated alpha-Gal syndrome (AGS). The results suggested that O. bursa is establishing across Mediterranean countries, and may increase the risk for gamasoidosis. The immune antibody response to mite proteins was higher for IgM and similar for IgE and IgG antibodies between patients and non-allergic control individuals exposed to mite or tick bites. The anti-α-Gal antibody levels were similar between patients and controls, a result supported by the absence of this carbohydrate in mites. These results suggested that mite bites do not correlate with antibody response to acarine proteins or α-Gal, and are not associated with the AGS.
Jose Francisco Lima Barbero; Marta Sánchez Sánchez; Alejandro Cabezas-Cruz; Lourdes Mateos-Hernández; Marinela Contreras; Isabel G. Fernández De Mera; Margarita Villar; José De La Fuente. Clinical gamasoidosis and antibody response in two patients infested with Ornithonyssus bursa (Acari: Gamasida: Macronyssidae). Experimental and Applied Acarology 2019, 78, 555 -564.
AMA StyleJose Francisco Lima Barbero, Marta Sánchez Sánchez, Alejandro Cabezas-Cruz, Lourdes Mateos-Hernández, Marinela Contreras, Isabel G. Fernández De Mera, Margarita Villar, José De La Fuente. Clinical gamasoidosis and antibody response in two patients infested with Ornithonyssus bursa (Acari: Gamasida: Macronyssidae). Experimental and Applied Acarology. 2019; 78 (4):555-564.
Chicago/Turabian StyleJose Francisco Lima Barbero; Marta Sánchez Sánchez; Alejandro Cabezas-Cruz; Lourdes Mateos-Hernández; Marinela Contreras; Isabel G. Fernández De Mera; Margarita Villar; José De La Fuente. 2019. "Clinical gamasoidosis and antibody response in two patients infested with Ornithonyssus bursa (Acari: Gamasida: Macronyssidae)." Experimental and Applied Acarology 78, no. 4: 555-564.
Ticks and tick-borne pathogens affect health and welfare of companion animals worldwide, and some human tick-borne diseases are associated with exposure to domestic animals. Vaccines are the most environmentally friendly alternative to acaracides for the control of tick infestations, and to reduce the risk for tick-borne diseases affecting human and animal health. However, vaccines have not been developed or successfully implemented for most vector-borne diseases. The main limitation for the development of effective vaccines is the identification of protective antigens. To address this limitation, in this study we used an experimental approach combining vaccinomics based on transcriptomics and proteomics data with vaccination trials for the identification of tick protective antigens. The study was focused on Ixodes ricinus and Dermacentor reticulatus that infest humans, companion animals and other domestic and wild animals, and transmit disease-causing pathogens. Tick larvae and adult salivary glands were selected for analysis to target tick organs and developmental stages playing a key role during tick life cycle and pathogen infection and transmission. Two I. ricinus (heme lipoprotein and uncharacterized secreted protein) and five D. reticulatus (glypican-like protein, secreted protein involved in homophilic cell adhesion, sulfate/anion exchanger, signal peptidase complex subunit 3, and uncharacterized secreted protein) proteins were identified as the most effective protective antigens based on the criteria of vaccine E > 80%. The putative function of selected protective antigens, which are involved in different biological processes, resulted in vaccines affecting multiple tick developmental stages. These results suggested that the combination of some of these antigens might be considered to increase vaccine efficacy through antigen synergy for the control of tick infestations and potentially affecting pathogen infection and transmission. These antigens were proposed for commercial vaccine development for the control of tick infestations in companion animals, and potentially in other hosts for these tick species.
Marinela Contreras; Margarita Villar; José de la Fuente. A Vaccinomics Approach for the Identification of Tick Protective Antigens for the Control of Ixodes ricinus and Dermacentor reticulatus Infestations in Companion Animals. Frontiers in Physiology 2019, 10, 977 .
AMA StyleMarinela Contreras, Margarita Villar, José de la Fuente. A Vaccinomics Approach for the Identification of Tick Protective Antigens for the Control of Ixodes ricinus and Dermacentor reticulatus Infestations in Companion Animals. Frontiers in Physiology. 2019; 10 ():977.
Chicago/Turabian StyleMarinela Contreras; Margarita Villar; José de la Fuente. 2019. "A Vaccinomics Approach for the Identification of Tick Protective Antigens for the Control of Ixodes ricinus and Dermacentor reticulatus Infestations in Companion Animals." Frontiers in Physiology 10, no. : 977.
The poultry red mite, Dermanyssus gallinae (De Geer, 1778), is a worldwide distributed ectoparasite and considered a major pest affecting the laying hen industry in Europe. Based on available information in other ectoparasites, the mite microbiome might participate in several biological processes and the acquisition, maintenance and transmission of pathogens. However, little is known about the role of poultry red mite in the transmission of pathogenic bacteria as a mechanical carrier or a biological vector. Herein, we used a metaproteomics approach to characterize the alphaproteobacteria in the microbiota of poultry red mite, and variations in its profile with ectoparasite development (nymphs vs. adults) and feeding (unfed vs. fed). The results showed that the bacterial community associated with D. gallinae was mainly composed of environmental and commensal bacteria. Putative symbiotic bacteria of the genera Wolbachia, C. Tokpelaia and Sphingomonas were identified, together with potential pathogenic bacteria of the genera Inquilinus, Neorickettsia and Roseomonas. Significant differences in the composition of alphaproteobacterial microbiota were associated with mite development and feeding, suggesting that bacteria have functional implications in metabolic pathways associated with blood feeding. These results support the use of metaproteomics for the characterization of alphaproteobacteria associated with the D. gallinae microbiota that could provide relevant information for the understanding of mite-host interactions and the development of potential control interventions. Research Highlights - Metaproteomics is a valid approach for microbiome characterization in ectoparasites. - Alphaproteobacteria putative bacterial symbionts were identified in D. gallinae. - Mite development and feeding were related to variations in bacterial community. - Potentially pathogenic bacteria were identified in mite microbiota.
José Francisco Lima-Barbero; Sandra Díaz-Sanchez; Olivier Sparagano; Robert D. Finn; José De La Fuente; Margarita Villar. Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778). Avian Pathology 2019, 48, S52 -S59.
AMA StyleJosé Francisco Lima-Barbero, Sandra Díaz-Sanchez, Olivier Sparagano, Robert D. Finn, José De La Fuente, Margarita Villar. Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778). Avian Pathology. 2019; 48 (sup1):S52-S59.
Chicago/Turabian StyleJosé Francisco Lima-Barbero; Sandra Díaz-Sanchez; Olivier Sparagano; Robert D. Finn; José De La Fuente; Margarita Villar. 2019. "Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778)." Avian Pathology 48, no. sup1: S52-S59.
Intracerebral inoculation of extracts from post-mortem human Alzheimer’s disease brains into mice produces a prion-like spreading effect of amyloid-β. The differences observed between these extracts and the synthetic peptide, in terms of amyloid-β internalization and seed and cell-to-cell transmission of cytosolic protein aggregates, suggest that brain extracts contain key contributors that enhance the prion-like effect of amyloid-β. Nevertheless, these potential partners are still unknown due to the complexity of whole brain extracts. Herein, we established a method based on sequential detergent solubilization of post-mortem samples of human brains affected by Alzheimer’s disease that strongly enrich amyloid-β aggregates by eliminating 92% of the remaining proteins. Internalization of Aβ1–42 from the enriched AD extracts was evaluated in vitro, and internalization of fluorescent-labeled AD extracts was also investigated in vivo. Furthermore, we carried out a molecular characterization of the Aβ-enriched fraction using label-free proteomics, studying the distribution of representative components in the amygdala and the olfactory cortex of additional human AD brain samples by immunohistochemistry. Aβ1–42 from the enriched AD extracts are internalized into endothelial cells in vitro after 48 h. Furthermore, accumulation of fluorescent-labeled Aβ-enriched extracts into mouse microglia was observed in vivo after 4 months of intracerebral inoculation. Label-free proteomics (FDR < 0.01) characterization of the amyloid-β-enriched fraction from different post-mortem samples allowed for the identification of more than 130 proteins, several of which were significantly overrepresented (i.e., ANXA5 and HIST1H2BK; p < 0.05) and underrepresented (i.e., COL6A or FN1; p < 0.05) in the samples with Alzheimer’s disease. We were also able to identify proteins exclusively observed in Alzheimer’s disease (i.e., RNF213) or only detected in samples not affected by the disease (i.e., CNTN1) after the enrichment process. Immunohistochemistry against these proteins in additional tissues revealed their particular distribution in the amygdala and the olfactory cortex in relation to the amyloid-β plaque. Identification and characterization of the unique features of these extracts, in terms of amyloid-β enrichment, identification of the components, in vitro and in vivo cell internalization, and tissue distribution, constitute the best initial tool to further investigate the seeding and transmissibility proposed in the prion-like hypothesis of Alzheimer’s disease.
Cristina M Pedrero-Prieto; Alicia Flores-Cuadrado; Daniel Saiz-Sánchez; Isabel Úbeda-Bañón; Javier Frontiñan; Francisco J. Alcaín; Lourdes Mateos-Hernández; José De La Fuente; Mario Durán-Prado; Margarita Villar; Alino Martínez-Marcos; Juan R. Peinado. Human amyloid-β enriched extracts: evaluation of in vitro and in vivo internalization and molecular characterization. Alzheimer's Research & Therapy 2019, 11, 1 -16.
AMA StyleCristina M Pedrero-Prieto, Alicia Flores-Cuadrado, Daniel Saiz-Sánchez, Isabel Úbeda-Bañón, Javier Frontiñan, Francisco J. Alcaín, Lourdes Mateos-Hernández, José De La Fuente, Mario Durán-Prado, Margarita Villar, Alino Martínez-Marcos, Juan R. Peinado. Human amyloid-β enriched extracts: evaluation of in vitro and in vivo internalization and molecular characterization. Alzheimer's Research & Therapy. 2019; 11 (1):1-16.
Chicago/Turabian StyleCristina M Pedrero-Prieto; Alicia Flores-Cuadrado; Daniel Saiz-Sánchez; Isabel Úbeda-Bañón; Javier Frontiñan; Francisco J. Alcaín; Lourdes Mateos-Hernández; José De La Fuente; Mario Durán-Prado; Margarita Villar; Alino Martínez-Marcos; Juan R. Peinado. 2019. "Human amyloid-β enriched extracts: evaluation of in vitro and in vivo internalization and molecular characterization." Alzheimer's Research & Therapy 11, no. 1: 1-16.
The microRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms including innate and adaptive immune pathways to control bacterial, parasite and viral infections, and pathogens could modify host miRNA profile to facilitate infection and multiplication. Therefore, understanding the function of host miRNAs in response to pathogen infection is relevant to characterize host-pathogen molecular interactions and to provide new targets for effective new interventions for the control infectious diseases. The objective of this study was to characterize the dynamics and functional significance of the miRNA response of the tick vector Ixodes scapularis in response to Anaplasma phagocytophilum infection, the causative agent of human and animal granulocytic anaplasmosis. To address this objective, the composition of tick miRNAs, functional annotation, and expression profiling was characterized using high throughout RNA sequencing in uninfected and A. phagocytophilum-infected I. scapularis ISE6 tick cells, a model for tick hemocytes involved in pathogen infection. The results provided new evidences on the role of tick miRNA during pathogen infection, and showed that A. phagocytophilum modifies I. scapularis tick cell miRNA profile and upregulates isc-mir-79 to facilitate infection by targeting the Roundabout protein 2 (Robo2) pathway. Furthermore, these results suggested new targets for interventions to control pathogen infection in ticks.
Sara Artigas Jerónimo; Pilar Alberdi; Margarita Villar Rayo; Alejandro Cabezas-Cruz; Pedro J. Espinosa Prados; Lourdes Mateos-Hernández; José De La Fuente. Anaplasma phagocytophilum modifies tick cell microRNA expression and upregulates isc-mir-79 to facilitate infection by targeting the Roundabout protein 2 pathway. Scientific Reports 2019, 9, 1 -15.
AMA StyleSara Artigas Jerónimo, Pilar Alberdi, Margarita Villar Rayo, Alejandro Cabezas-Cruz, Pedro J. Espinosa Prados, Lourdes Mateos-Hernández, José De La Fuente. Anaplasma phagocytophilum modifies tick cell microRNA expression and upregulates isc-mir-79 to facilitate infection by targeting the Roundabout protein 2 pathway. Scientific Reports. 2019; 9 (1):1-15.
Chicago/Turabian StyleSara Artigas Jerónimo; Pilar Alberdi; Margarita Villar Rayo; Alejandro Cabezas-Cruz; Pedro J. Espinosa Prados; Lourdes Mateos-Hernández; José De La Fuente. 2019. "Anaplasma phagocytophilum modifies tick cell microRNA expression and upregulates isc-mir-79 to facilitate infection by targeting the Roundabout protein 2 pathway." Scientific Reports 9, no. 1: 1-15.
Ticks act as vectors of pathogens affecting human and animal health worldwide, and recent research has focused on the characterization of tick-pathogen interactions using omics technologies to identify new targets for developing novel control interventions. The regulome (transcription factors-target genes interactions) plays a critical role in cell response to pathogen infection. Therefore, the application of regulomics to tick-pathogen interactions would advance our understanding of these molecular interactions and contribute to the identification of novel control targets for the prevention and control of tick infestations and tick-borne diseases. However, limited information is available on the role of tick regulome in response to pathogen infection. In this study, we applied complementary in silico approaches to modeling how Anaplasma phagocytophilum infection modulates tick vector regulome. This proof-of-concept research provided support for the use of network analysis in the study of regulome response to infection, resulting in new information on tick-pathogen interactions and potential targets for developing interventions for the control of tick infestations and pathogen transmission. Deciphering the precise nature of circuits that shape the tick regulome in response to pathogen infection is an area of research that in the future will advance our knowledge of tick-pathogen interactions, and the identification of new antigens for the control of tick infestations and pathogen infection/transmission.
Sara Artigas Jerónimo; Agustín Estrada-Peña; Alejandro Cabezas-Cruz; Pilar Alberdi; Margarita Villar; José De La Fuente. Modeling Modulation of the Tick Regulome in Response to Anaplasma phagocytophilum for the Identification of New Control Targets. Frontiers in Physiology 2019, 10, 1 .
AMA StyleSara Artigas Jerónimo, Agustín Estrada-Peña, Alejandro Cabezas-Cruz, Pilar Alberdi, Margarita Villar, José De La Fuente. Modeling Modulation of the Tick Regulome in Response to Anaplasma phagocytophilum for the Identification of New Control Targets. Frontiers in Physiology. 2019; 10 ():1.
Chicago/Turabian StyleSara Artigas Jerónimo; Agustín Estrada-Peña; Alejandro Cabezas-Cruz; Pilar Alberdi; Margarita Villar; José De La Fuente. 2019. "Modeling Modulation of the Tick Regulome in Response to Anaplasma phagocytophilum for the Identification of New Control Targets." Frontiers in Physiology 10, no. : 1.