This paper describes the complete findings of the EU-funded research project OPTIMISC, which investigated methods to optimize the production and use of miscanthus biomass. Miscanthus bioenergy and bioproduct chains were investigated by trialing fifteen diverse germplasm types in a range of climatic and soil environments across central Europe, Ukraine, Russia and China. The abiotic stress tolerances of a wider panel of 100 germplasm types to drought, salinity and low temperatures were measured in the laboratory and a field trial in Belgium. A small selection of germplasm types was evaluated for performance in grasslands on marginal sites in Germany and the UK. The growth traits underlying biomass yield and quality were measured to improve regional estimates of feedstock availability. Several potential high-value bioproducts were identified. The combined results provide recommendations to policymakers, growers and industry. The major technical advances in miscanthus production achieved by OPTIMISC include: 1) demonstration that novel hybrids can out-yield the standard commercially grown genotype Miscanthus x giganteus; 2) characterisation of the interactions of physiological growth responses with environmental variation within and between sites; 3) quantification of biomass-quality-relevant traits; 4) abiotic stress tolerances of miscanthus genotypes; 5) selections suitable for production on marginal land; 6) field establishment methods for seeds using plugs; 7) evaluation of harvesting methods; and 8) quantification of energy used in densification (pellet) technologies with a range of hybrids with differences in stem wall properties. End-user needs were addressed by demonstrating the potential of optimizing miscanthus biomass composition for the production of ethanol and biogas as well as for combustion. The costs and life-cycle assessment of seven miscanthus-based value chains, including small- and large-scale heat and power, ethanol, biogas and insulation material production, revealed GHG-emission- and fossil-energy-saving potentials of up to 30.6 t CO2eq C ha-1y-1 and 429 GJ ha-1y-1 , respectively. Transport distance was identified as an important cost factor. Negative carbon mitigation costs of -78 € t-1 CO2eq C were recorded for local biomass use. The OPTIMISC results demonstrate the potential of miscanthus as a crop for marginal sites and provide information and technologies for the commercial implementation of miscanthus-based value chains.

Farmers, scientists, and other soil health stakeholders require interpretable indicators of soil hydraulic function. Determining which indicators to use has been difficult because of measurement disconformity, spatial and temporal variability, recently established treatments, and the effect of site characteristics on management practice differences. The North American Project to Evaluate Soil Health Measurements includes 124 sites uniformly sampled across a range of soil health management practices in North America in 2019. We compare and recommend indicators of hydraulic function that best characterize soil health. We assessed the relationship of each indicator to a suite of soil inherent properties and climate variables, the response of each indicator to soil health management practices, the effect that soil inherent properties (clay content, sand content, and pH) and climatic variables (10‐yr mean annual precipitation and temperature) had on response to management practices, and the relationship among the responses of the indicators to soil health management practices. Field capacity measured on intact cores (θ _{FC_INTACT} ) was the best measure of soil hydraulic function, because it responded to management, represents a direct measure of soil hydraulic function, is proximal to stakeholder values, and its response to management was not significantly influenced by inherent and climatic variables. Other suitable indicators are bulk density, soil organic carbon (SOC), and aggregate stability, which are not direct measures of soil hydraulic function but do respond to management and may be practical in situations in which measuring θ _{FC_INTACT} is not. This study informs selection of soil health indicators to measure soil hydraulic function.

Currently accepted pedotransfer functions show negligible effect of management‐induced changes to soil organic carbon (SOC) on plant available water holding capacity (θ _AWHC ), while some studies show the ability to substantially increase θ _AWHC through management. The Soil Health Institute's North America Project to Evaluate Soil Health Measurements measured water content at field capacity using intact soil cores across 124 long‐term research sites that contained increases in SOC as a result of management treatments such as reduced tillage and cover cropping. Pedotransfer functions were created for volumetric water content at field capacity (θ _FC ) and permanent wilting point (θ _PWP ). New pedotransfer functions had predictions of θ _AWHC that were similarly accurate compared with Saxton and Rawls when tested on samples from the National Soil Characterization database. Further, the new pedotransfer functions showed substantial effects of soil calcareousness and SOC on θ _AWHC . For an increase in SOC of 10 g kg ^–1 (1%) in noncalcareous soils, an average increase in θ _AWHC of 3.0 mm 100 mm ^–1 soil (0.03 m ³ m ^–3 ) on average across all soil texture classes was found. This SOC related increase in θ _AWHC is about double previous estimates. Calcareous soils had an increase in θ _AWHC of 1.2 mm 100 mm ^–1 soil associated with a 10 g kg ^–1 increase in SOC, across all soil texture classes. New equations can aid in quantifying benefits of soil management practices that increase SOC and can be used to model the effect of changes in management on drought resilience.

Various soil health indicators that measure a chemically defined fraction of nitrogen (N) or a process related to N cycling have been proposed to quantify the potential to supply N to crops, a key soil function. We evaluated five N indicators (total soil N, autoclavable citrate extractable N, water‐extractable organic N, potentially mineralizable N, and N ‐acetyl‐β‐ D ‐glucosaminidase activity) at 124 sites with long‐term experiments across North America evaluating a variety of managements. We found that 59%–81% of the variation in N indicators was among sites, with indicator values decreasing with temperature and increasing with precipitation and clay content. The N indicators increased from 6%–39% in response to decreasing tillage, cover cropping, retaining residue, and applying organic sources of nutrients. Overall, increasing the quantity of organic inputs, whether from increased residue retention, cover cropping, or rotations with higher biomass, resulted in higher values of the N indicators. Although N indicators responded to management in similar ways, the analysis cost and availability of testing laboratories is highly variable. Further, given the strong relationships of the N indicators with carbon (C) indicators, measuring soil organic C along with 24‐h potential C mineralization could be used as a proxy for N supply instead of measuring potentially mineralizable N or any other N indicator directly.

Weed management is one of the main challenges of conservation agriculture. Although all three components of conservation agriculture (minimal tillage, permanent soil cover and crop diversification) can reduce weed populations, these effects may only become apparent in the medium to long term. This study evaluated weed biomass, density and diversity with and without herbicide control in a long-term trial initiated in 1991 in the Mexican Highlands to evaluate all three components of conservation agriculture. Data were collected in 2004, 2005, 2013, 2014 and 2015. Weed density and biomass were generally lower in conservation agriculture than with conventional tillage. The three components of conservation agriculture significantly reduced weed biomass, which was lower when all three components were applied together. When herbicides were applied, weed biomass in conservation agriculture was 91% lower in maize and 81% lower in wheat than in conventional tillage. Different treatments favored different weed species, but no trend toward increased perennial weeds was observed in conservation agriculture. These data supported claims stating that if adequate weed control is achieved in the initial years, weed populations in conservation agriculture systems are lower than in conventional tillage systems.