This article presents a complete test equipment for the promising on-board serial high-speed SpaceFibre protocol, published by the European Committee for Space Standardization. SpaceFibre and SpaceWire are standard communication protocols for the latest technology sensor devices intended for on-board satellites and spacecrafts in general, especially for sensors based on image acquisition, such as scanning radiometers or star-tracking devices. The new design aims to provide the enabling tools to the scientific community and the space industry in order to promote the adoption of open standards in space on-board communications for current- and future-generation spacecraft missions. It is the first instrument expressly designed for LabVIEW users, and it offers tools and advanced features for the test and development of new SpaceFibre devices. In addition, it supports the previous SpaceWire standard and cross-communications. Thanks to novel cutting-edge design methods, the system complex architecture can be implemented on natively supported LabVIEW programmable devices. The presented system is highly customizable in terms of interface support and is provided with a companion LabVIEW application and LabVIEW Application Programming Interface (API) for user custom automated test-chains. It offers real-time capabilities and supports data rates up to 6.25 Gbps.The proposed solutions is then fairly compared with other currently available SpaceFibre test equipment. Its comprehensiveness and modularity make it suitable for either on-board device developments or spacecraft system integrations.