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Plants are affected by numerous environmental factors that influence their physiological processes and productivity. Early revealing of their action based on measuring spectra of reflected light and calculating reflectance indices is an important stage in the protection of agricultural plants. Photochemical reflectance index (PRI) is a widely used parameter related to photosynthetic changes in plants under action of stressors. We developed a new system for proximal imaging of PRI based on using short pulses of measuring light detected simultaneously in green (530 nm) and yellow (570 nm) spectral bands. The system has several advances compared to those reported in literature. Active light illumination and subtraction of the ambient light allow for PRI measurements without periodic calibrations. Short duration of measuring pulses (18 ms) minimizes their influence on plants. Measurements in two spectral bands operated by separate cameras with aligned fields of visualization allow one to exclude mechanically switchable parts like filter wheels thus minimizing acquisition time and increasing durability of the setup. Absolute values of PRI and light-induced changes in PRI (ΔPRI) in pea leaves and changes of these parameters under action of light with different intensities, water shortage, and heating have been investigated using the developed setup. Changes in ΔPRI are shown to be more robust than the changes in the absolute value of PRI which is in a good agreement with our previous studies. Values of PRI and, especially, ΔPRI are strongly linearly related to the energy-dependent component of the non-photochemical quenching and can be potentially used for estimation of this component. Additionally, we demonstrate that the developed system can also measure fast changes in PRI (hundreds of milliseconds and seconds) under leaf illumination by the pulsed green-yellow measuring light. Thus, the developed system of proximal PRI imaging can be used for PRI measurements (including fast changes in PRI) and estimation of stressors-induced photosynthetic changes.
Vladimir Sukhov; Ekaterina Sukhova; Andrey Khlopkov; Lyubov Yudina; Anastasiia Ryabkova; Alexander Telnykh; Ekaterina Sergeeva; Vladimir Vodeneev; Ilya Turchin. Proximal Imaging of Changes in Photochemical Reflectance Index in Leaves Based on Using Pulses of Green-Yellow Light. Remote Sensing 2021, 13, 1762 .
AMA StyleVladimir Sukhov, Ekaterina Sukhova, Andrey Khlopkov, Lyubov Yudina, Anastasiia Ryabkova, Alexander Telnykh, Ekaterina Sergeeva, Vladimir Vodeneev, Ilya Turchin. Proximal Imaging of Changes in Photochemical Reflectance Index in Leaves Based on Using Pulses of Green-Yellow Light. Remote Sensing. 2021; 13 (9):1762.
Chicago/Turabian StyleVladimir Sukhov; Ekaterina Sukhova; Andrey Khlopkov; Lyubov Yudina; Anastasiia Ryabkova; Alexander Telnykh; Ekaterina Sergeeva; Vladimir Vodeneev; Ilya Turchin. 2021. "Proximal Imaging of Changes in Photochemical Reflectance Index in Leaves Based on Using Pulses of Green-Yellow Light." Remote Sensing 13, no. 9: 1762.
Local damage (e.g., burning) induces a variation potential (VP), which is an important electrical signal in higher plants. A VP propagates into undamaged parts of the plant and influences numerous physiological processes, including photosynthesis. Rapidly increasing plant tolerance to stressors is likely to be a result of the physiological changes. Thus, developing methods of revealing VP-induced physiological changes can be used for the remote sensing of plant systemic responses to local damage. Previously, we showed that burning-induced VP influenced a photochemical reflectance index in pea leaves, but the influence of the electrical signals on other reflectance indices was not investigated. In this study, we performed a complex analysis of the influence of VP induction by local burning on difference reflectance indices based on 400–700 nm wavelengths in leaves of pea seedlings. Heat maps of the significance of local burning-induced changes in the reflectance indices and their correlations with photosynthetic parameters were constructed. Large spectral regions with significant changes in these indices after VP induction were revealed. Most changes were strongly correlated to photosynthetic parameters. Some indices, which can be potentially effective for revealing local burning-induced photosynthetic changes, are separately shown. Our results show that difference reflectance indices based on 400–700 nm wavelengths can potentially be used for the remote sensing of plant systemic responses induced by local damages and subsequent propagation of VPs.
Ekaterina Sukhova; Lyubov Yudina; Ekaterina Gromova; Anastasiia Ryabkova; Vladimir Vodeneev; Vladimir Sukhov. Influence of Local Burning on Difference Reflectance Indices Based on 400–700 nm Wavelengths in Leaves of Pea Seedlings. Plants 2021, 10, 878 .
AMA StyleEkaterina Sukhova, Lyubov Yudina, Ekaterina Gromova, Anastasiia Ryabkova, Vladimir Vodeneev, Vladimir Sukhov. Influence of Local Burning on Difference Reflectance Indices Based on 400–700 nm Wavelengths in Leaves of Pea Seedlings. Plants. 2021; 10 (5):878.
Chicago/Turabian StyleEkaterina Sukhova; Lyubov Yudina; Ekaterina Gromova; Anastasiia Ryabkova; Vladimir Vodeneev; Vladimir Sukhov. 2021. "Influence of Local Burning on Difference Reflectance Indices Based on 400–700 nm Wavelengths in Leaves of Pea Seedlings." Plants 10, no. 5: 878.
In the natural fluidic environment of a biological system, nanoparticles swiftly adsorb plasma proteins on their surface forming a “protein corona”, which profoundly and often adversely affects their residence in the systemic circulation in vivo and their interaction with cells in vitro. It has been recognized that preformation of a protein corona under controlled conditions ameliorates the protein corona effects, including colloidal stability in serum solutions. We report on the investigation of the stabilizing effects of a denatured bovine serum albumin (dBSA) protein corona formed on the surface of upconversion nanoparticles (UCNPs). UCNPs were chosen as a nanoparticle model due to their unique photoluminescent properties suitable for background-free biological imaging and sensing. UCNP surface was modified with nitrosonium tetrafluoroborate (NOBF4) to render it hydrophilic. UCNP-NOBF4 nanoparticles were incubated in dBSA solution to form a dBSA corona followed up by lyophilization. As produced dBSA-UCNP-NOBF4 demonstrated high photoluminescence brightness, sustained colloidal stability after long-term storage and the reduced level of serum protein surface adsorption. These results show promise of dBSA-based nanoparticle pretreatment to improve the amiability to biological environments towards theranostic applications.
Samah Shanwar; Liuen Liang; Andrey Nechaev; Daria Bausheva; Irina Balalaeva; Vladimir Vodeneev; Indrajit Roy; Andrei Zvyagin; Evgenii Guryev. Controlled Formation of a Protein Corona Composed of Denatured BSA on Upconversion Nanoparticles Improves Their Colloidal Stability. Materials 2021, 14, 1657 .
AMA StyleSamah Shanwar, Liuen Liang, Andrey Nechaev, Daria Bausheva, Irina Balalaeva, Vladimir Vodeneev, Indrajit Roy, Andrei Zvyagin, Evgenii Guryev. Controlled Formation of a Protein Corona Composed of Denatured BSA on Upconversion Nanoparticles Improves Their Colloidal Stability. Materials. 2021; 14 (7):1657.
Chicago/Turabian StyleSamah Shanwar; Liuen Liang; Andrey Nechaev; Daria Bausheva; Irina Balalaeva; Vladimir Vodeneev; Indrajit Roy; Andrei Zvyagin; Evgenii Guryev. 2021. "Controlled Formation of a Protein Corona Composed of Denatured BSA on Upconversion Nanoparticles Improves Their Colloidal Stability." Materials 14, no. 7: 1657.
Variation potential (VP) is an important long-distance electrical signal in higher plants that is induced by local damages, influences numerous physiological processes, and participates in plant adaptation to stressors. The transmission of increased hydraulic pressure through xylem vessels is the probable mechanism of VP propagation in plants; however, the rates of the pressure transmission and VP propagation can strongly vary. We analyzed this problem on the basis of a simple mathematical model of the pressure distribution along a xylem vessel, which was approximated by a tube with a pressure gradient. It is assumed that the VP is initiated if the integral over pressure is more than a threshold one, taking into account that the pressure is transiently increased in the initial point of the tube and is kept constant in the terminal point. It was shown that this simple model can well describe the parameters of VP propagation in higher plants, including the increase in time before VP initiation and the decrease in the rate of VP propagation with an increase in the distance from the zone of damage. Considering three types of the pressure dynamics, our model predicts that the velocity of VP propagation can be stimulated by an increase in the length of a plant shoot and also depends on pressure dynamics in the damaged zone. Our results theoretically support the hypothesis about the impact of pressure variations in xylem vessels on VP propagation.
Ekaterina Sukhova; Elena Akinchits; Sergey Gudkov; Roman Pishchalnikov; Vladimir Vodeneev; Vladimir Sukhov. A Theoretical Analysis of Relations between Pressure Changes along Xylem Vessels and Propagation of Variation Potential in Higher Plants. Plants 2021, 10, 372 .
AMA StyleEkaterina Sukhova, Elena Akinchits, Sergey Gudkov, Roman Pishchalnikov, Vladimir Vodeneev, Vladimir Sukhov. A Theoretical Analysis of Relations between Pressure Changes along Xylem Vessels and Propagation of Variation Potential in Higher Plants. Plants. 2021; 10 (2):372.
Chicago/Turabian StyleEkaterina Sukhova; Elena Akinchits; Sergey Gudkov; Roman Pishchalnikov; Vladimir Vodeneev; Vladimir Sukhov. 2021. "A Theoretical Analysis of Relations between Pressure Changes along Xylem Vessels and Propagation of Variation Potential in Higher Plants." Plants 10, no. 2: 372.
Mechanisms of the specific systemic response of plant to different adverse factors are poorly understood. We studied the mechanisms acting in wheat (Triticum aestivum L.) under the action of local burn and gradual heating. Both stimuli induce a variation potential (VP) propagation and a biphasic (fast and long-term phases) photosynthetic response in non-stimulated zones of plant with stimulus-specific parameters of the latter: the fast phase or long-term phase predominance in responses induced by burn or heating, respectively. The burn-induced VP and photosynthetic response attenuate with distance, while the heating-induced VP and photosynthetic response were of more stable amplitude in distant part of the stimulated plant. VP propagation in both cases induced apoplast alkalization with dynamics well corresponding to such of VP and of the fast phase of photosynthetic response. Gradual heating induced a significant rise in jasmonate production along with a decrease in stomatal conductance with characteristic times well corresponding to the long-term phase of the photosynthetic response. We suppose that the VP-induced pH shift is responsible for in the induction of the fast phase, while jasmonate production for the long-term phase of the photosynthetic response. The revealed differences in the systemic response to stressors studied, apparently, reflect two distinct plant adaptation strategies to fast and slow-growing stimuli. The immediate response in the tissue nearest to the damage zone is the most important under a fast-growing stimulus. The fundamentally different situation is under a slowly-growing stimulus which provokes long-term changes in the plant that ensure the preparation of the whole organism for impending environmental changes.
Maxim Mudrilov; Maria Ladeynova; Ekaterina Berezina; Marina Grinberg; Anna Brilkina; Vladimir Sukhov; Vladimir Vodeneev. Mechanisms of specific systemic response in wheat plants under different locally acting heat stimuli. Journal of Plant Physiology 2021, 258-259, 153377 .
AMA StyleMaxim Mudrilov, Maria Ladeynova, Ekaterina Berezina, Marina Grinberg, Anna Brilkina, Vladimir Sukhov, Vladimir Vodeneev. Mechanisms of specific systemic response in wheat plants under different locally acting heat stimuli. Journal of Plant Physiology. 2021; 258-259 ():153377.
Chicago/Turabian StyleMaxim Mudrilov; Maria Ladeynova; Ekaterina Berezina; Marina Grinberg; Anna Brilkina; Vladimir Sukhov; Vladimir Vodeneev. 2021. "Mechanisms of specific systemic response in wheat plants under different locally acting heat stimuli." Journal of Plant Physiology 258-259, no. : 153377.
Photosynthesis is an important target of action of numerous environmental factors; in particular, stressors can strongly affect photosynthetic light reactions. Considering relations of photosynthetic light reactions to electron and proton transport, it can be supposed that extremely low frequency magnetic field (ELFMF) may influence these reactions; however, this problem has been weakly investigated. In this paper, we experimentally tested a hypothesis about the potential influence of ELFMF of 18 µT intensity with Schumann resonance frequencies (7.8, 14.3, and 20.8 Hz) on photosynthetic light reactions in wheat and pea seedlings. It was shown that ELFMF decreased non-photochemical quenching in wheat and weakly influenced quantum yield of photosystem II at short-term treatment; in contrast, the changes in potential and effective quantum yields of photosystem II were observed mainly under chronic action of ELFMF. It is interesting that both short-term and chronic treatment decreased the time periods for 50% activation of quantum yield and non-photochemical quenching under illumination. Influence of ELFMF on pea was not observed at both short-term and chronic treatment. Thus, we showed that ELFMF with Schumann resonance frequencies could influence photosynthetic light processes; however, this effect depends on plant species (wheat or pea) and type of treatment (short-term or chronic).
Vladimir Sukhov; Ekaterina Sukhova; Yulia Sinitsyna; Ekaterina Gromova; Natalia Mshenskaya; Anastasiia Ryabkova; Nikolay Ilin; Vladimir Vodeneev; Evgeny Маreev; Colin Price. Influence of Magnetic Field with Schumann Resonance Frequencies on Photosynthetic Light Reactions in Wheat and Pea. Cells 2021, 10, 149 .
AMA StyleVladimir Sukhov, Ekaterina Sukhova, Yulia Sinitsyna, Ekaterina Gromova, Natalia Mshenskaya, Anastasiia Ryabkova, Nikolay Ilin, Vladimir Vodeneev, Evgeny Маreev, Colin Price. Influence of Magnetic Field with Schumann Resonance Frequencies on Photosynthetic Light Reactions in Wheat and Pea. Cells. 2021; 10 (1):149.
Chicago/Turabian StyleVladimir Sukhov; Ekaterina Sukhova; Yulia Sinitsyna; Ekaterina Gromova; Natalia Mshenskaya; Anastasiia Ryabkova; Nikolay Ilin; Vladimir Vodeneev; Evgeny Маreev; Colin Price. 2021. "Influence of Magnetic Field with Schumann Resonance Frequencies on Photosynthetic Light Reactions in Wheat and Pea." Cells 10, no. 1: 149.
Plants are unavoidably exposed to ionizing radiation (IR) from natural and anthropogenic sources. Along with the chronic irradiation, plants are influenced by other environmental stress factors, to which they must adapt. We suppose that high background radiation modifies plant response to environmental stressors by influencing the stress signaling which are the crucial players in induction and orchestrating the formation of plant adaptation. Chronic β-radiation (31.3 μGy/h) does not cause pronounced oxidative stress and significant changes in morphometric parameters of wheat seedlings (Triticum aestivum L.). At the same time, chronic IR exposure causes slight activation of transpiration and assimilation, and activates H+-ATPase-dependent transmembrane transport. In irradiated plants, there is an increase in the amplitude, velocity and propagation distance of electrical signals induced by a local stimulus. Accordingly, in irradiated plants, photosynthesis and transpiration responses caused by an electrical signal are significantly enhanced. The opposite effect of the electric signal on heat stress resistance was found: after the signal passing, the resistance of control plants increases, but it decreases for irradiated plants. It is assumed that the inversion of the effect is resulted from the enhanced responses of transpiration due to the electrical signals amplification.
Marina A. Grinberg; Sergey V. Gudkov; Irina V. Balalaeva; Ekaterina Gromova; Yuliya Sinitsyna; Vladimir Sukhov; Vladimir Vodeneev. Effect of chronic β-radiation on long-distance electrical signals in wheat and their role in adaptation to heat stress. Environmental and Experimental Botany 2021, 184, 104378 .
AMA StyleMarina A. Grinberg, Sergey V. Gudkov, Irina V. Balalaeva, Ekaterina Gromova, Yuliya Sinitsyna, Vladimir Sukhov, Vladimir Vodeneev. Effect of chronic β-radiation on long-distance electrical signals in wheat and their role in adaptation to heat stress. Environmental and Experimental Botany. 2021; 184 ():104378.
Chicago/Turabian StyleMarina A. Grinberg; Sergey V. Gudkov; Irina V. Balalaeva; Ekaterina Gromova; Yuliya Sinitsyna; Vladimir Sukhov; Vladimir Vodeneev. 2021. "Effect of chronic β-radiation on long-distance electrical signals in wheat and their role in adaptation to heat stress." Environmental and Experimental Botany 184, no. : 104378.
Electrical signals in plants caused by external stimuli are capable of inducing various physiological responses. The mechanisms of transformation of a long-distance electrical signal (ES) into a functional response remain largely unexplored and require additional research. In this work, we investigated the role of calcium ions in the development of ES-induced respiratory response. Gradual heating of the leaf causes the propagation of variation potential (VP) in the pea seedling. The propagation of VP leads to a transient activation of respiration in an unaffected leaf. During the VP generation, a transient increase in the intracellular calcium concentration takes place. A calcium channel blocker inhibits the respiratory response, and a calcium ionophore induces the activation of respiration. Inhibitory analysis has showed that the VP-induced increase in respiration activity is probably associated with calcium-mediated activation of rotenone-insensitive alternative NADPH dehydrogenases in mitochondria.
Andrey Khlopkov; Oksana Sherstneva; Maria Ladeynova; Marina Grinberg; Lyubov Yudina; Vladimir Sukhov; Vladimir Vodeneev. Participation of calcium ions in induction of respiratory response caused by variation potential in pea seedlings. Plant Signaling & Behavior 2021, 16, 1869415 .
AMA StyleAndrey Khlopkov, Oksana Sherstneva, Maria Ladeynova, Marina Grinberg, Lyubov Yudina, Vladimir Sukhov, Vladimir Vodeneev. Participation of calcium ions in induction of respiratory response caused by variation potential in pea seedlings. Plant Signaling & Behavior. 2021; 16 (4):1869415.
Chicago/Turabian StyleAndrey Khlopkov; Oksana Sherstneva; Maria Ladeynova; Marina Grinberg; Lyubov Yudina; Vladimir Sukhov; Vladimir Vodeneev. 2021. "Participation of calcium ions in induction of respiratory response caused by variation potential in pea seedlings." Plant Signaling & Behavior 16, no. 4: 1869415.
Local damage (e.g., burning, heating, or crushing) causes the generation and propagation of a variation potential (VP), which is a unique electrical signal in higher plants. A VP influences numerous physiological processes, with photosynthesis and respiration being important targets. VP generation is based on transient inactivation of H+-ATPase in plasma membrane. In this work, we investigated the participation of this inactivation in the development of VP-induced photosynthetic and respiratory responses. Two- to three-week-old pea seedlings (Pisum sativum L.) and their protoplasts were investigated. Photosynthesis and respiration in intact seedlings were measured using a GFS-3000 gas analyzer, Dual-PAM-100 Pulse-Amplitude-Modulation (PAM)-fluorometer, and a Dual-PAM gas-exchange Cuvette 3010-Dual. Electrical activity was measured using extracellular electrodes. The parameters of photosynthetic light reactions in protoplasts were measured using the Dual-PAM-100; photosynthesis- and respiration-related changes in O2 exchange rate were measured using an Oxygraph Plus System. We found that preliminary changes in the activity of H+-ATPase in the plasma membrane (its inactivation by sodium orthovanadate or activation by fusicoccin) influenced the amplitudes and magnitudes of VP-induced photosynthetic and respiratory responses in intact seedlings. Decreases in H+-ATPase activity (sodium orthovanadate treatment) induced fast decreases in photosynthetic activity and increases in respiration in protoplasts. Thus, our results support the effect of H+-ATPase inactivation on VP-induced photosynthetic and respiratory responses.
Lyubov Yudina; Oksana Sherstneva; Ekaterina Sukhova; Marina Grinberg; Sergey Mysyagin; Vladimir Vodeneev; Vladimir Sukhov. Inactivation of H+-ATPase Participates in the Influence of Variation Potential on Photosynthesis and Respiration in Peas. Plants 2020, 9, 1585 .
AMA StyleLyubov Yudina, Oksana Sherstneva, Ekaterina Sukhova, Marina Grinberg, Sergey Mysyagin, Vladimir Vodeneev, Vladimir Sukhov. Inactivation of H+-ATPase Participates in the Influence of Variation Potential on Photosynthesis and Respiration in Peas. Plants. 2020; 9 (11):1585.
Chicago/Turabian StyleLyubov Yudina; Oksana Sherstneva; Ekaterina Sukhova; Marina Grinberg; Sergey Mysyagin; Vladimir Vodeneev; Vladimir Sukhov. 2020. "Inactivation of H+-ATPase Participates in the Influence of Variation Potential on Photosynthesis and Respiration in Peas." Plants 9, no. 11: 1585.
A local leaf burning causes variation potential (VP) propagation, a decrease in photosynthesis activity, and changes in the content of phytohormones in unstimulated leaves in pea plants. The VP-induced photosynthesis response develops in two phases: fast inactivation and long-term inactivation. Along with a decrease in photosynthetic activity, there is a transpiration suppression in unstimulated pea leaves, which corresponds to the long-term phase of photosynthesis response. Phytohormone level analysis showed an increase in the concentration of jasmonic acid (JA) preceding a transpiration suppression and a long-term phase of the photosynthesis response. Analysis of the spatial and temporal dynamics of electrical signals, phytohormone levels, photosynthesis, and transpiration activity showed the most pronounced changes in the more distant leaf from the area of local stimulation. The established features are related to the architecture of the vascular bundles in the pea stem.
Maria Ladeynova; Maxim Mudrilov; Ekaterina Berezina; Dmitry Kior; Marina Grinberg; Anna Brilkina; Vladimir Sukhov; Vladimir Vodeneev. Spatial and Temporal Dynamics of Electrical and Photosynthetic Activity and the Content of Phytohormones Induced by Local Stimulation of Pea Plants. Plants 2020, 9, 1364 .
AMA StyleMaria Ladeynova, Maxim Mudrilov, Ekaterina Berezina, Dmitry Kior, Marina Grinberg, Anna Brilkina, Vladimir Sukhov, Vladimir Vodeneev. Spatial and Temporal Dynamics of Electrical and Photosynthetic Activity and the Content of Phytohormones Induced by Local Stimulation of Pea Plants. Plants. 2020; 9 (10):1364.
Chicago/Turabian StyleMaria Ladeynova; Maxim Mudrilov; Ekaterina Berezina; Dmitry Kior; Marina Grinberg; Anna Brilkina; Vladimir Sukhov; Vladimir Vodeneev. 2020. "Spatial and Temporal Dynamics of Electrical and Photosynthetic Activity and the Content of Phytohormones Induced by Local Stimulation of Pea Plants." Plants 9, no. 10: 1364.
Abscisic acid (ABA) is an important hormone in plants that participates in their acclimation to the action of stressors. Treatment by exogenous ABA and its synthetic analogs are a potential way of controlling the tolerance of agricultural plants; however, the mechanisms of influence of the ABA treatment on photosynthetic processes require further investigations. The aim of our work was to investigate the participation of inactivation of the plasma membrane H+-ATP-ase on the influence of ABA treatment on photosynthetic processes and their regulation by electrical signals in peas. The ABA treatment of seedlings was performed by spraying them with aqueous solutions (10−5 M). The combination of a Dual-PAM-100 PAM fluorometer and GFS-3000 infrared gas analyzer was used for photosynthetic measurements; the patch clamp system on the basis of a SliceScope Pro 2000 microscope was used for measurements of electrical activity. It was shown that the ABA treatment stimulated the cyclic electron flow around photosystem I and decreased the photosynthetic CO2 assimilation, the amplitude of burning-induced electrical signals (variation potentials), and the magnitude of photosynthetic responses relating to these signals; in contrast, treatment with exogenous ABA increased the heat tolerance of photosynthesis. An investigation of the influence of ABA treatment on the metabolic component of the resting potential showed that this treatment decreased the activity of the H+-ATP-ase in the plasma membrane. Inhibitor analysis using sodium orthovanadate demonstrated that this decrease may be a mechanism of the ABA treatment-induced changes in photosynthetic processes, their heat tolerance, and regulation by electrical signals.
Lyubov Yudina; Ekaterina Sukhova; Oksana Sherstneva; Marina Grinberg; Maria Ladeynova; Vladimir Vodeneev; Vladimir Sukhov. Exogenous Abscisic Acid Can Influence Photosynthetic Processes in Peas through a Decrease in Activity of H+-ATP-ase in the Plasma Membrane. Biology 2020, 9, 324 .
AMA StyleLyubov Yudina, Ekaterina Sukhova, Oksana Sherstneva, Marina Grinberg, Maria Ladeynova, Vladimir Vodeneev, Vladimir Sukhov. Exogenous Abscisic Acid Can Influence Photosynthetic Processes in Peas through a Decrease in Activity of H+-ATP-ase in the Plasma Membrane. Biology. 2020; 9 (10):324.
Chicago/Turabian StyleLyubov Yudina; Ekaterina Sukhova; Oksana Sherstneva; Marina Grinberg; Maria Ladeynova; Vladimir Vodeneev; Vladimir Sukhov. 2020. "Exogenous Abscisic Acid Can Influence Photosynthetic Processes in Peas through a Decrease in Activity of H+-ATP-ase in the Plasma Membrane." Biology 9, no. 10: 324.
Electrical signals (ESs) can be induced by local action of stressors in plants; they influence numerous physiological processes (photosynthesis, transpiration, respiration, genes expression, synthesis of phytohormones, etc.) and, thereby, induce a systemic adaptation response. Development of optical methods of a remote sensing of this response can be important for agricultural and ecological monitoring. Preliminarily, we showed (Sukhova et al., Plant Sign Behav 2019; 14:e1610301) that burning-induced ESs induced changes in leaf reflectance at broad spectral bands (400-500, 500-600, 600-700, and 700-800 nm). The aims of the present work were (i) investigation of ESs influence on difference reflectance indices (RIs) calculated on the basis of these broad spectral bands and (ii) analysis of connection of the indices with water content in plants. Pea seedlings were investigated. ESs were induced by burning of the first mature leaf; ESs had high amplitudes in the second leaf and had low amplitudes in the fourth leaf. It was shown that ESs induced significant changes in RIs, which were calculated on basis of intensities of the reflected light at (i) 400-500 and 600-700 nm, (ii) 500-600 and 700-800 nm, and (iii) 600-700 and 700-800 nm. The effect was strong in the second leaf and weak in the fourth leaf; that is, the response was dependent on the magnitude of ESs. ESs-induced changes in RI were strongly connected with ESs-induced decrease of leaf water content which was estimated on basis of decrease of water index. Thus, broadband RIs can be used for revealing the ESs-induced systemic stress response in plants.
Ekaterina Sukhova; Lyubov Yudina; Ekaterina Gromova; Vladimir Nerush; Vladimir Vodeneev; Vladimir Sukhov. Burning-induced electrical signals influence broadband reflectance indices and water index in pea leaves. Plant Signaling & Behavior 2020, 15, 1737786 .
AMA StyleEkaterina Sukhova, Lyubov Yudina, Ekaterina Gromova, Vladimir Nerush, Vladimir Vodeneev, Vladimir Sukhov. Burning-induced electrical signals influence broadband reflectance indices and water index in pea leaves. Plant Signaling & Behavior. 2020; 15 (4):1737786.
Chicago/Turabian StyleEkaterina Sukhova; Lyubov Yudina; Ekaterina Gromova; Vladimir Nerush; Vladimir Vodeneev; Vladimir Sukhov. 2020. "Burning-induced electrical signals influence broadband reflectance indices and water index in pea leaves." Plant Signaling & Behavior 15, no. 4: 1737786.
The remote sensing of a plant’s physiological state is a key problem of precision agriculture. The photochemical reflectance index (PRI), which is based on the intensities of the reflected light at 531 and 570 nm, is an important tool for the remote sensing of photosynthetic processes in plants. In particular, the PRI can be strongly connected with the non-photochemical quenching of chlorophyll fluorescence (NPQ) and the quantum yield of photosystem II (ФPSII); however, this connection is dependent on illumination, the intensity of stressor actions, the time scale of measurements, etc. The aim of the present work was to analyze the connection of PRI with the energy-dependent component of NPQ (NPQF) and ФPSII under heating and soil drought conditions. Pea, wheat, and pumpkin seedlings, which were grown under controlled conditions, were investigated. A PAM fluorometer Dual-PAM-100 and spectrometer S-100 were used for measurements of photosynthetic parameters and PRI, respectively. It was shown that heat stress increased the NPQF and the magnitude of light-induced changes in PRI (ΔPRI) and decreased ФPSII in pea seedlings. The decreased ФPSII and increased ΔPRI were observed in wheat after heating, but significant changes in NPQF were absent; the significant decrease in ФPSII was observed in pumpkin seedlings, while there were no significant changes in the other parameters. ΔPRI and NPQF after heating were significantly correlated. However, a significant correlation of the absolute values of PRI with photosynthetic parameters was absent. The soil drought increased NPQF and the magnitude of ΔPRI and decreased ФPSII in peas. ΔPRI was strongly correlated with photosynthetic parameters, but this correlation was absent for the absolute value of PRI. Thus, ΔPRI is strongly connected with the magnitude of NPQF and can be used as an estimator of this parameter.
Lyubov Yudina; Ekaterina Sukhova; Ekaterina Gromova; Vladimir Nerush; Vladimir Vodeneev; Vladimir Sukhov. A light-induced decrease in the photochemical reflectance index (PRI) can be used to estimate the energy-dependent component of non-photochemical quenching under heat stress and soil drought in pea, wheat, and pumpkin. Photosynthesis Research 2020, 146, 175 -187.
AMA StyleLyubov Yudina, Ekaterina Sukhova, Ekaterina Gromova, Vladimir Nerush, Vladimir Vodeneev, Vladimir Sukhov. A light-induced decrease in the photochemical reflectance index (PRI) can be used to estimate the energy-dependent component of non-photochemical quenching under heat stress and soil drought in pea, wheat, and pumpkin. Photosynthesis Research. 2020; 146 (1-3):175-187.
Chicago/Turabian StyleLyubov Yudina; Ekaterina Sukhova; Ekaterina Gromova; Vladimir Nerush; Vladimir Vodeneev; Vladimir Sukhov. 2020. "A light-induced decrease in the photochemical reflectance index (PRI) can be used to estimate the energy-dependent component of non-photochemical quenching under heat stress and soil drought in pea, wheat, and pumpkin." Photosynthesis Research 146, no. 1-3: 175-187.
Smart agriculture management systems with real-time control of plant health and vegetation are recognized as one of the crucial technologies determining agriculture development, playing a fundamental role in reducing yield losses and improving product quality. The earliest plant responses to various adverse factors are propagating stress signals, including electrical ones, and the changes in physiological processes induced by them. Among the latter, photosynthesis is of particular interest due to its key role in the production process. Of practical importance, photosynthesis activity can be registered not only in contact mode but by remote sensing using optical methods. The aim of the present work was to develop the approach to automatic determination of the main parameters of electrical signals and changes in photosynthesis activity and transpiration using continuous wavelet transform (CWT). Applying CWT based on derivatives of the Gaussian function allows accurate determination of the parameters of electrical signals as well as induced physiological responses. Moreover, CWT was applied for spatio-temporal mapping of the photosynthesis response to stress factors in pea leaf. The offered approach allowed automatic identification of the response start time in every pixel and visualization of the change propagation front. The results indicate high potential of CWT for automatic assessment of plants stress, including monitoring of plant health in large-scale agricultural lands and automated fields.
Maxim Mudrilov; Lyubov Katicheva; Maria Ladeynova; Irina Balalaeva; Vladimir Sukhov; Vladimir Vodeneev. Automatic Determination of the Parameters of Electrical Signals and Functional Responses of Plants Using the Wavelet Transformation Method. Agriculture 2019, 10, 7 .
AMA StyleMaxim Mudrilov, Lyubov Katicheva, Maria Ladeynova, Irina Balalaeva, Vladimir Sukhov, Vladimir Vodeneev. Automatic Determination of the Parameters of Electrical Signals and Functional Responses of Plants Using the Wavelet Transformation Method. Agriculture. 2019; 10 (1):7.
Chicago/Turabian StyleMaxim Mudrilov; Lyubov Katicheva; Maria Ladeynova; Irina Balalaeva; Vladimir Sukhov; Vladimir Vodeneev. 2019. "Automatic Determination of the Parameters of Electrical Signals and Functional Responses of Plants Using the Wavelet Transformation Method." Agriculture 10, no. 1: 7.
An analysis of photosynthetic response on action of stressors is an important problem, which can be solved by experimental and theoretical methods, including mathematical modeling of photosynthetic processes. The aim of our work was elaboration of a mathematical model, which simulated development of a nonphotochemical quenching under different light conditions. We analyzed two variants of the model: the first variant included a light-induced activation of the electron transport chain; in contrast, the second variant did not describe this activation. Both variants of the model described interactions between transitions from open reaction centers to closed ones (and vice versa) and development of the nonphotochemical quenching. Investigation of both variants of the model showed well qualitative and quantitative accordance between simulated and experimental changes in coefficient of the nophotochemical quenching which were analyzed under different light regimes: (i) the stepped increase of the light intensity without dark intervals between steps, (ii) periodical illuminations by different light intensities with constant durations which were separated by constant dark intervals, and (iii) periodical illuminations by the constant light intensity with different durations which were separated by different dark intervals. Thus, the model can be used for theoretical prediction of stress changes in photosynthesis under fluctuations in light intensity and search of optimal regimes of plant illumination.
Ekaterina Sukhova; Andrey Khlopkov; Vladimir Vodeneev; Vladimir Sukhov. Simulation of a nonphotochemical quenching in plant leaf under different light intensities. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms 2019, 1861, 148138 .
AMA StyleEkaterina Sukhova, Andrey Khlopkov, Vladimir Vodeneev, Vladimir Sukhov. Simulation of a nonphotochemical quenching in plant leaf under different light intensities. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. 2019; 1861 (2):148138.
Chicago/Turabian StyleEkaterina Sukhova; Andrey Khlopkov; Vladimir Vodeneev; Vladimir Sukhov. 2019. "Simulation of a nonphotochemical quenching in plant leaf under different light intensities." Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms 1861, no. 2: 148138.
High-contrast optical imaging is achievable using phosphorescent labels to suppress the short-lived background due to the optical backscatter and autofluorescence. However, the long-lived phosphorescence is generally incompatible with high-speed laser-scanning imaging modalities. Here, we show that upconversion nanoparticles of structure NaYF4:Yb co-doped with 8% Tm (8T-UCNP) in combination with a commercial laser-scanning multiphoton microscopy are uniquely suited for labeling biological systems to acquire high-resolution images with the enhanced contrast. In comparison with many phosphorescent labels, the 8T-UCNP emission lifetime of ∼ 15 µs affords rapid image acquisition. The high-order optical nonlinearity of the 8T-UCNP (n ≈ 4, as confirmed experimentally and theoretically) afforded pushing the resolution limit attainable with UCNPs to the diffraction-limit. The contrast enhancement was achieved by suppressing the background using (i) bandpass spectral filtering of the narrow emission peak of 8T-UCNP at 455-nm, and (ii) time-gating implemented with a time-correlated single-photon counting system that demonstrated the contrast enhancement of > 2.5-fold of polyethyleneimine-coated 8T-UCNPs taken up by human breast adenocarcinoma cells SK-BR-3. As a result, discrete 8T-UCNP nanoparticles became clearly observable in the freshly excised spleen tissue of laboratory mice 15-min post intravenous injection of an 8T-UCNP solution. The demonstrated approach paves the way for high-contrast, high-resolution, and high-speed multiphoton microscopy in challenging environments of intense autofluorescence, exogenous staining, and turbidity, as typically occur in intravital imaging. Open image in new window
Alexey B. Kostyuk; Artem D. Vorotnov; Andrey Ivanov; Arthur B. Volovetskiy; Aleksandr V. Kruglov; Lyudmila M. Sencha; Liuen Liang; Evgenii Guryev; Vladimir Vodeneev; Sergey M. Deyev; Yiqing Lu; Andrei V. Zvyagin. Resolution and contrast enhancement of laser-scanning multiphoton microscopy using thulium-doped upconversion nanoparticles. Nano Research 2019, 12, 2933 -2940.
AMA StyleAlexey B. Kostyuk, Artem D. Vorotnov, Andrey Ivanov, Arthur B. Volovetskiy, Aleksandr V. Kruglov, Lyudmila M. Sencha, Liuen Liang, Evgenii Guryev, Vladimir Vodeneev, Sergey M. Deyev, Yiqing Lu, Andrei V. Zvyagin. Resolution and contrast enhancement of laser-scanning multiphoton microscopy using thulium-doped upconversion nanoparticles. Nano Research. 2019; 12 (12):2933-2940.
Chicago/Turabian StyleAlexey B. Kostyuk; Artem D. Vorotnov; Andrey Ivanov; Arthur B. Volovetskiy; Aleksandr V. Kruglov; Lyudmila M. Sencha; Liuen Liang; Evgenii Guryev; Vladimir Vodeneev; Sergey M. Deyev; Yiqing Lu; Andrei V. Zvyagin. 2019. "Resolution and contrast enhancement of laser-scanning multiphoton microscopy using thulium-doped upconversion nanoparticles." Nano Research 12, no. 12: 2933-2940.
Measurement of the photochemical reflectance index (PRI) is a simple and non-invasive method for the evaluation of photosynthetic processes in higher plants. At the same time, the relationship between photosynthetic parameters and PRI can be significantly modified at the initial stages of illumination; thus, the study of the mechanisms of development of PRI after the beginning of illumination is an important task. The aim of this work was to analyze the relationship between the acidification of chloroplast lumen, which was assessed as an increase in the absorption of light by the leaf at a wavelength of 535 nm (light scattering, LS), and changes in PRI under short-term illumination in the leaves of pea and geranium. It was shown that the illumination caused an increase in LS and a decrease in PRI in both studied objects. Significant differences were found for the amplitude of the photochemical reflectance index decrease, while the differences in the absolute values of PRI at different stages of illumination were not reliable. Correlation analysis showed that the increase in LS during the first two minutes of illumination, reflecting light-induced acidification of chloroplast lumen, strongly correlated with the amplitude of the decrease in PRI; at the same time, at later stages of illumination, such a relationship was absent. Additional analysis carried out on geranium showed that the cessation of illumination caused the opposite dynamics: the decrease in LS was accompanied by an increase in PRI. The results show that the changes in PRI at the first minutes after the beginning of illumination, apparently, were due to the acidification of the chloroplast lumen.
E. M. Sukhova; L. M. Yudina; Vladimir Vodeneev; V. S. Sukhov. Analysis of Changes in Photochemical Reflectance Index (PRI) in Relation to the Acidification of the Lumen of the Chloroplasts of Pea and Geranium Leaves under a Short-Term Illumination. Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology 2019, 13, 243 -252.
AMA StyleE. M. Sukhova, L. M. Yudina, Vladimir Vodeneev, V. S. Sukhov. Analysis of Changes in Photochemical Reflectance Index (PRI) in Relation to the Acidification of the Lumen of the Chloroplasts of Pea and Geranium Leaves under a Short-Term Illumination. Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology. 2019; 13 (3):243-252.
Chicago/Turabian StyleE. M. Sukhova; L. M. Yudina; Vladimir Vodeneev; V. S. Sukhov. 2019. "Analysis of Changes in Photochemical Reflectance Index (PRI) in Relation to the Acidification of the Lumen of the Chloroplasts of Pea and Geranium Leaves under a Short-Term Illumination." Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology 13, no. 3: 243-252.
The purpose of this investigation is to create a scientific basis and technology of the production of fluoropolymer photoconversion films for greenhouses to improve the performance of greenhouses in the area of risk farming. The aim basis of photoconversion technology in greenhouses is reduced to photoconversion of UV radiation into blue-violet, and green and yellow light into red light necessary for plants. In other words, when sunlight passes through fluoropolymer photoconversion films, the intensity of the blue-violet and red regions of the spectrum should increase, and the intensity of the UV, green and yellow regions of the spectrum should drop. The article presents the manufacturing technology of fluoropolymer photoconversion films and examples of its use in greenhouses.
Sergey Gudkov; A V Simakin; V E Ivanov; E V Barmina; I V Baimler; I I Rakov; L A Katicheva; Vladimir Vodeneev; G A Shafeev. Creation and application of fluoropolymer photoconversion films for greenhouses: Concept. IOP Conference Series: Materials Science and Engineering 2019, 525, 012087 .
AMA StyleSergey Gudkov, A V Simakin, V E Ivanov, E V Barmina, I V Baimler, I I Rakov, L A Katicheva, Vladimir Vodeneev, G A Shafeev. Creation and application of fluoropolymer photoconversion films for greenhouses: Concept. IOP Conference Series: Materials Science and Engineering. 2019; 525 (1):012087.
Chicago/Turabian StyleSergey Gudkov; A V Simakin; V E Ivanov; E V Barmina; I V Baimler; I I Rakov; L A Katicheva; Vladimir Vodeneev; G A Shafeev. 2019. "Creation and application of fluoropolymer photoconversion films for greenhouses: Concept." IOP Conference Series: Materials Science and Engineering 525, no. 1: 012087.
The study of effects of ionizing radiation (IR) on plants is important in relation to several problems: (I) the existence of zones where background radiation - either natural or technogenic - is increased; (II) the problems of space biology; (III) the use of IR in agricultural selection; (IV) general biological problems related to the fundamental patterns and specifics of the effects of IR on various living organisms. By now, researchers have accumulated and systematized a large body of data on the effects of IR on the growth and reproduction of plants, as well as on the changes induced by IR at the genetic level. At the same time, there is a large gap in understanding the mechanisms of IR influence on the biochemical and physiological processes - despite the fact that these processes form the basis determining the manifestation of IR effects at the level of the whole organism. On the one hand, the activity of physiological processes determines the growth of plants; on the other, it is determined by changes at the genetic level. Thus, it is the study of IR effects at the physiological and biochemical levels that can give the most detailed and complex picture of IR action in plants. The review focuses on the effects of radiation on the essential physiological processes, including photosynthesis, respiration, long-distance transport, the functioning of the hormonal system, and various biosynthetic processes. On the basis of a large body of experimental data, we analyze dose and time dependences of the IR-induced effects - which are qualitatively similar - on various physiological and biochemical processes. We also consider the sequence of stages in the development of those effects and discuss their mechanisms, as well as the cause-effect relationships between them. The primary IR-induced physicochemical reactions include the formation of various forms of reactive oxygen species (ROS) and are the cause of the observed changes in the functional activity of plants. The review emphasizes the role of hydrogen peroxide, a long-lived ROS, not only as a damaging agent, but also as a mediator - a universal intracellular messenger, which provides for the mechanism of long-distance signaling. A supposition is made that IR affects physiological processes mainly by violating the regulation of their activity. The violation seems to become possible due to the fact that there exists a crosstalk between different signaling systems of plants, such as ROS, calcium, hormonal and electrical systems. As a result of both acute and chronic irradiation, an increase in the level of ROS can influence the activity of a wide range of physiological processes - by regulating them both at the genetic and physiological levels. To understand the ways, by which IR affects plant growth and development, one needs detailed knowledge about the mechanisms of the processes that occur at the (i) genetic and (ii) physiological levels, as well as their interplay and (iii) knowledge about regulation of these processes at different levels.
Sergey V. Gudkov; Marina A. Grinberg; Vladimir Sukhov; Vladimir Vodeneev. Effect of ionizing radiation on physiological and molecular processes in plants. Journal of Environmental Radioactivity 2019, 202, 8 -24.
AMA StyleSergey V. Gudkov, Marina A. Grinberg, Vladimir Sukhov, Vladimir Vodeneev. Effect of ionizing radiation on physiological and molecular processes in plants. Journal of Environmental Radioactivity. 2019; 202 ():8-24.
Chicago/Turabian StyleSergey V. Gudkov; Marina A. Grinberg; Vladimir Sukhov; Vladimir Vodeneev. 2019. "Effect of ionizing radiation on physiological and molecular processes in plants." Journal of Environmental Radioactivity 202, no. : 8-24.
Local action of stressors induces generation and propagation of electrical signals (ESs), which influence numerous physiological processes (including photosynthesis, expression of genes, production of phytohormones, etc.) in undamaged parts of plants; i.e. they induce a systemic stress response. Development of methods of remote sensing of this response (in particular, optical methods) is an important practical task for agricultural and ecological monitoring. However, this problem is not sufficiently researched. Earlier, we reported that ESs influence the photochemical reflectance index, which can be calculated on the basis of reflected light at 531 and 570 nm, and these changes are connected with photosynthetic changes. The aim of the current work is investigation of the influence of ESs on reflectance at broad spectral bands (400–500 nm, 500–600 nm, 600–700 nm and 700–800 nm). We showed that burning-induced ESs caused transient increase of intensity of reflected light at the all investigated spectral bands of visible light: reflectance at 600–700 nm had the maximal magnitude of changes and reflectance at 700–800 nm had the minimal magnitude of changes. Dynamics of the reflectance changes were distinguished from dynamics of photosynthetic changes, induced by ESs; i.e. ESs-induced changes in reflectance seem to be weakly connected with the photosynthetic response. Thus, our results show that changes in reflectance at broad spectral bands can also be used for remote sensing of the ESs-induced systemic stress response in plants.
Ekaterina Sukhova; Lyubov Yudina; Elena Akinchits; Vladimir Vodeneev; Vladimir Sukhov. Influence of electrical signals on pea leaf reflectance in the 400–800-nm range. Plant Signaling & Behavior 2019, 14, 1610301 .
AMA StyleEkaterina Sukhova, Lyubov Yudina, Elena Akinchits, Vladimir Vodeneev, Vladimir Sukhov. Influence of electrical signals on pea leaf reflectance in the 400–800-nm range. Plant Signaling & Behavior. 2019; 14 (7):1610301.
Chicago/Turabian StyleEkaterina Sukhova; Lyubov Yudina; Elena Akinchits; Vladimir Vodeneev; Vladimir Sukhov. 2019. "Influence of electrical signals on pea leaf reflectance in the 400–800-nm range." Plant Signaling & Behavior 14, no. 7: 1610301.