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Marine sediments serve as a sink for contaminants of anthropogenic origin. Here, 25 major and trace elements were determined in surface and core sediments from an urban lagoon (Yundang Lagoon), China. The median concentrations of Pb, Cd, Cu, and Zn in both surface and core sediments exceeded global and crustal averages. Principal component analysis for the elements and ecological impact of the heavy metals indicated spatial heterogeneity in core sediments from different lagoon areas; however, no such pattern was observed in surface sediments. Geodetector analysis indicated spatial locations of lakes, pH, N%, C%, and S% as the major factors influencing the heterogeneity of potential ecological risk index, a cumulative measure of the ecological impact of heavy metal. The interaction detector indicated nonlinear and bivariate enhancement between different physicochemical parameters. Besides, a depth profile of the elements in different samples was also elucidated.
Philomina O. Peter; Azhar Rashid; François Nkinahamira; Hongjie Wang; Qian Sun; Mahmoud Gad; Chang-Ping Yu; Anyi Hu. Integrated assessment of major and trace elements in surface and core sediments from an urban lagoon, China: Potential ecological risks and influencing factors. Marine Pollution Bulletin 2021, 170, 112651 .
AMA StylePhilomina O. Peter, Azhar Rashid, François Nkinahamira, Hongjie Wang, Qian Sun, Mahmoud Gad, Chang-Ping Yu, Anyi Hu. Integrated assessment of major and trace elements in surface and core sediments from an urban lagoon, China: Potential ecological risks and influencing factors. Marine Pollution Bulletin. 2021; 170 ():112651.
Chicago/Turabian StylePhilomina O. Peter; Azhar Rashid; François Nkinahamira; Hongjie Wang; Qian Sun; Mahmoud Gad; Chang-Ping Yu; Anyi Hu. 2021. "Integrated assessment of major and trace elements in surface and core sediments from an urban lagoon, China: Potential ecological risks and influencing factors." Marine Pollution Bulletin 170, no. : 112651.
Sewage and fecal pollution cause antibiotic resistance genes (ARGs) pollution in urban lagoons. Seasonality also affects ARG dynamics. However, knowledge of factors controlling ARG community assembly across seasons is still limited. Here, we revealed the seasonal variation of ARGs and depict the underlying assembly processes and drivers via high-throughput quantitative PCR in an urban lagoon, China. A higher richness and abundance of ARGs were observed in summer and winter compared to spring and fall (Kruskal-Wallis test, P < 0.05). Both ARG and prokaryotic communities were mainly governed by stochastic processes, however, these processes drove ARGs and prokaryotes differently across seasons. Stochastic processes played a more dominant role in shaping ARG communities in summer (average stochasticity: 83%) and winter (75%), resulting in a stable antibiotic resistome. However, no such seasonal pattern of stochastic processes was determined for prokaryotes, indicating a decoupling of the assembly process of ARGs and prokaryotes. Moreover, fecal microorganisms (e.g., Bacteroidetes and Faecalibacterium) mediated the stochastic processes of ARG profiles, via enhancement of prokaryotic biomass and mobile genetic element abundances. The tnpA-07 transposase was the key for the horizontal gene transfer. These findings will enhance our understanding of how fecal pollution shapes ARG community assembly in urban lagoons.
Liyuan Hou; Hongjie Wang; Qingfu Chen; Jian-Qiang Su; Mahmoud Gad; Jiangwei Li; Sikandar I. Mulla; Chang-Ping Yu; Anyi Hu. Fecal pollution mediates the dominance of stochastic assembly of antibiotic resistome in an urban lagoon (Yundang lagoon), China. Journal of Hazardous Materials 2021, 417, 126083 .
AMA StyleLiyuan Hou, Hongjie Wang, Qingfu Chen, Jian-Qiang Su, Mahmoud Gad, Jiangwei Li, Sikandar I. Mulla, Chang-Ping Yu, Anyi Hu. Fecal pollution mediates the dominance of stochastic assembly of antibiotic resistome in an urban lagoon (Yundang lagoon), China. Journal of Hazardous Materials. 2021; 417 ():126083.
Chicago/Turabian StyleLiyuan Hou; Hongjie Wang; Qingfu Chen; Jian-Qiang Su; Mahmoud Gad; Jiangwei Li; Sikandar I. Mulla; Chang-Ping Yu; Anyi Hu. 2021. "Fecal pollution mediates the dominance of stochastic assembly of antibiotic resistome in an urban lagoon (Yundang lagoon), China." Journal of Hazardous Materials 417, no. : 126083.
Understanding the underlying mechanism that drives the microbial community mediated by substrates is crucial to enhance the biostimulation in trichloroethene (TCE)-contaminated sites. Here, we investigated the performance of stable TCE-dechlorinating consortia by monitoring the variations in TCE-related metabolites and explored their underlying assembly mechanisms using 16S rDNA amplicon sequencing and bioinformatics analyses. The monitoring results indicated that three stable TCE-dechlorinating consortia were successfully enriched by lactate-containing anaerobic media. The statistical analysis results demonstrated that the microbial communities of the enrichment cultures changed along with time and were distinguished by their sample sources. The deterministic and stochastic processes were simultaneously responsible for shaping the TCE-dechlorinating community assembly. The indicator patterns shifted with the exhaustion of the carbon source and the pollutants, and the tceA-carrying Dehalococcoides, as an indicator for the final stage samples, responded positively to TCE removal during the incubation period. Pseudomonas, Desulforhabdus, Desulfovibrio and Methanofollis were identified as keystone populations in the TCE-dechlorinating process by co-occurrence network analysis. The results of this study indicate that lactate can be an effective substrate for stimulated bioremediation of TCE-contaminated sites, and the reduction of the stochastic forces or enhancement of the deterministic interventions may promote more effective biostimulation.
Jiangwei Li; Anyi Hu; Shijie Bai; Xiaoyong Yang; Qian Sun; Xu Liao; Chang-Ping Yu. Characterization and Performance of Lactate-Feeding Consortia for Reductive Dechlorination of Trichloroethene. Microorganisms 2021, 9, 751 .
AMA StyleJiangwei Li, Anyi Hu, Shijie Bai, Xiaoyong Yang, Qian Sun, Xu Liao, Chang-Ping Yu. Characterization and Performance of Lactate-Feeding Consortia for Reductive Dechlorination of Trichloroethene. Microorganisms. 2021; 9 (4):751.
Chicago/Turabian StyleJiangwei Li; Anyi Hu; Shijie Bai; Xiaoyong Yang; Qian Sun; Xu Liao; Chang-Ping Yu. 2021. "Characterization and Performance of Lactate-Feeding Consortia for Reductive Dechlorination of Trichloroethene." Microorganisms 9, no. 4: 751.
Rapid urbanization has resulted in pervasive occurrence of antibiotic resistance genes (ARGs) in urban aquatic ecosystems. However, limited information is available concerning the ARG profiles and the forces responsible for their assembly in urban landscape lagoon systems. Here, we employed high-throughput quantitative PCR (HT-qPCR) to characterize the spatial variations of ARGs in surface and core sediments of Yundang Lagoon, China. The results indicated that the average richness and absolute abundance of ARGs were 11 and 53 times higher in the lagoon sediments as compared to pristine reference Tibetan lake sediments, highlighting the role of anthropogenic activities in ARG pollution. Co-occurrence network analysis indicated that various anaerobic prokaryotic genera belonging to Alpha-, Deltaproteobacteria, Bacteroidetes, Euryarchaeota, Firmicutes and Synergistetes were the potential hosts of ARGs. The partial least squares-path modeling (PLS-PM) analysis revealed positive and negative indirect effects of physicochemical factors and heavy metals on the lagoon ARG profiles, via biotic factors, respectively. The horizontal (mediated by mobile genetic elements) and vertical (mediated by prokaryotic communities) gene transfer may directly contribute the most to drive the abundance and composition of ARGs, respectively. Furthermore, the neutral community model demonstrated that the assembly of sediment ARG communities was jointly governed by deterministic and stochastic processes. Overall, this study provides novel insights into the diversity and distribution of ARGs in the benthic habitat of urban lagoon systems and underlying mechanisms for the spread and proliferation of ARGs.
Hongjie Wang; Liyuan Hou; Yongqin Liu; Keshao Liu; Lanping Zhang; Fuyi Huang; Lin Wang; Azhar Rashid; Anyi Hu; Changping Yu. Horizontal and vertical gene transfer drive sediment antibiotic resistome in an urban lagoon system. Journal of Environmental Sciences 2020, 102, 11 -23.
AMA StyleHongjie Wang, Liyuan Hou, Yongqin Liu, Keshao Liu, Lanping Zhang, Fuyi Huang, Lin Wang, Azhar Rashid, Anyi Hu, Changping Yu. Horizontal and vertical gene transfer drive sediment antibiotic resistome in an urban lagoon system. Journal of Environmental Sciences. 2020; 102 ():11-23.
Chicago/Turabian StyleHongjie Wang; Liyuan Hou; Yongqin Liu; Keshao Liu; Lanping Zhang; Fuyi Huang; Lin Wang; Azhar Rashid; Anyi Hu; Changping Yu. 2020. "Horizontal and vertical gene transfer drive sediment antibiotic resistome in an urban lagoon system." Journal of Environmental Sciences 102, no. : 11-23.
The occurrence, dissemination and assembly processes of antibiotic resistance genes (ARGs) in urban water ecosystems are far from being understood. Here, we examined the diversity and abundance of ARGs in urban water ecosystems including landscape ponds, drinking water reservoirs, influents (IFs) and effluents (EFs) of wastewater treatment plants of a coastal city, China through high-throughput quantitative PCR. A total of 237 ARGs were identified, where multidrug, aminoglycoside and beta-lactamase resistance genes were the most abundant. Urban ponds had a comparatively high diversity and large numbers of shared ARGs with IFs and EFs. The average absolute abundance of ARGs (1.38 × 107 copies/mL) and mobile genetic elements (MGEs) (4.19 × 106 copies/mL) in ponds were only one order of magnitude lower than those of IFs, but higher than those of EFs and reservoirs. Stochastic processes dominated the ARG community assembly in IFs and ponds due to the random horizontal gene transfer caused by MGEs. These results imply that urban ponds are hotspots of ARGs. We further identified 25, 3, and 11 indicator ARGs for tracing the ARG contamination from IFs, EFs and ponds, respectively. Our study represents the first to highlight the role of urban ponds in the dissemination of ARGs.
Liyuan Hou; Lanping Zhang; Furun Li; Sijun Huang; Jun Yang; Cong Ma; Duanxin Zhang; Chang-Ping Yu; Anyi Hu. Urban ponds as hotspots of antibiotic resistome in the urban environment. Journal of Hazardous Materials 2020, 403, 124008 .
AMA StyleLiyuan Hou, Lanping Zhang, Furun Li, Sijun Huang, Jun Yang, Cong Ma, Duanxin Zhang, Chang-Ping Yu, Anyi Hu. Urban ponds as hotspots of antibiotic resistome in the urban environment. Journal of Hazardous Materials. 2020; 403 ():124008.
Chicago/Turabian StyleLiyuan Hou; Lanping Zhang; Furun Li; Sijun Huang; Jun Yang; Cong Ma; Duanxin Zhang; Chang-Ping Yu; Anyi Hu. 2020. "Urban ponds as hotspots of antibiotic resistome in the urban environment." Journal of Hazardous Materials 403, no. : 124008.
Microeukaryotic communities are sensitive to environmental changes; and are considered essential for microbial food webs and biogeochemical cycles. Therefore, understanding the community responses of microeukaryotes to environmental changes is of great ecological significance. Very little is known about the assembly mechanisms underlying the microeukaryotic communities, especially for the key ecological groups (e.g., habitat generalists/specialists) in the riverine ecosystems. Here, we employed 18S rDNA amplicon sequencing to study the assembly processes governing the microeukaryotic communities and their habitat generalists and specialists across three hydrological seasons in a subtropical river in China. The results showed that deterministic and stochastic processes jointly shaped the microeukaryotic communities, where the relative importance of stochastic processes decreased in the following order: wet > normal > dry seasons. However, deterministic processes played more important role in shaping the microeukaryotic communities than those of prokaryotes. Meanwhile, stochastic and deterministic processes were responsible for structuring the microeukaryotic habitat generalists and specialists, respectively. Generally, the pure effects of physicochemical factors on the microeukaryotic communities and their key ecological groups were ~ 1.7 folds than those of the micropollutants. However, several micropollutants (e.g., acetaminophen, benzophenone-3, bisphenol A, sulfadiazine, triclocarban and triclosan) were found to have a strong effect on the habitat specialists. Altogether, we suggested that the ecological responses of the riverine microeukaryotes to micropollutants may vary among species due to the intrinsic differences in their environmental plasticity.
Mahmoud Gad; Liyuan Hou; Jiangwei Li; Yang Wu; Azhar Rashid; Nengwang Chen; Anyi Hu. Distinct mechanisms underlying the assembly of microeukaryotic generalists and specialists in an anthropogenically impacted river. Science of The Total Environment 2020, 748, 141434 .
AMA StyleMahmoud Gad, Liyuan Hou, Jiangwei Li, Yang Wu, Azhar Rashid, Nengwang Chen, Anyi Hu. Distinct mechanisms underlying the assembly of microeukaryotic generalists and specialists in an anthropogenically impacted river. Science of The Total Environment. 2020; 748 ():141434.
Chicago/Turabian StyleMahmoud Gad; Liyuan Hou; Jiangwei Li; Yang Wu; Azhar Rashid; Nengwang Chen; Anyi Hu. 2020. "Distinct mechanisms underlying the assembly of microeukaryotic generalists and specialists in an anthropogenically impacted river." Science of The Total Environment 748, no. : 141434.
Heavy metal(loid)s and organic pollutants have garnered global concern due to their potential for bioaccumulation and carcinogenic effects. The aquatic environment is one of the most important receiving compartments for these pollutants. Therefore, researchers are committed to developing inexpensive and highly efficient technologies for the removal of these recalcitrant contaminants from water. Zero-valent iron (ZVI), which has a strong reduction ability, low cost and can be recycled, has been considered as an environmentally benign element for removal of heavy metal(loid)s and organic chlorinated pollutants. However, some factors limit the ZVI application, with passivation of ZVI considered as the main limitation. Recent research has focused on methods to eliminate or alleviate these interferences and maximize ZVI performance. This review summarizes recent findings for the removal of typical heavy metal(loid)s and organic pollutants in the aquatic environment by ZVI-based technologies. Different combinations of chemical, physical, biological, or other methods with ZVI and their operating conditions and influencing factors are presented and discussed. Finally, we also present the removal mechanisms of ZVI-based technologies and recommendations for future research. This review provides an up-to-date perspective on the removal of pollutants using ZVI-based technologies and collates references for future large-scale application to wastewater treatment.
Yang Wu; Chung-Yu Guan; Nicholas Griswold; Li-Yuan Hou; Xin Fang; Anyi Hu; Zhi-Qiang Hu; Chang-Ping Yu. Zero-valent iron-based technologies for removal of heavy metal(loid)s and organic pollutants from the aquatic environment: Recent advances and perspectives. Journal of Cleaner Production 2020, 277, 123478 .
AMA StyleYang Wu, Chung-Yu Guan, Nicholas Griswold, Li-Yuan Hou, Xin Fang, Anyi Hu, Zhi-Qiang Hu, Chang-Ping Yu. Zero-valent iron-based technologies for removal of heavy metal(loid)s and organic pollutants from the aquatic environment: Recent advances and perspectives. Journal of Cleaner Production. 2020; 277 ():123478.
Chicago/Turabian StyleYang Wu; Chung-Yu Guan; Nicholas Griswold; Li-Yuan Hou; Xin Fang; Anyi Hu; Zhi-Qiang Hu; Chang-Ping Yu. 2020. "Zero-valent iron-based technologies for removal of heavy metal(loid)s and organic pollutants from the aquatic environment: Recent advances and perspectives." Journal of Cleaner Production 277, no. : 123478.
Estuaries, being the transitional zones between freshwater and marine environments, are important for protecting and rehabilitating the aquatic environments. Sediments from freshwater and marine environments were studied in Jiulong River Estuary (JRE) in different years for rare earth elements (REEs) and some environmentally important metal and metalloid elements (MMEs). The concentration of REEs ranged from 0.11 (Tm) to 296.20 mg kg−1 (Ce), while that of MMEs ranged from 0.40 (Cd) to 86,000 mg kg−1 (Al). The temporal analysis indicated an increase of both REEs and MMEs contaminants from 2012 to 2018. Fractionation of REEs and Ce and Eu anomalies indicated natural weathering, erosion processes and changes in redox chemistry at the sampling sites. Spatial structure analysis showed relatively higher levels of both REEs and MMEs in the freshwater sediments. These variations among the sampling sites indicated different land use and anthropogenic activities. The values of enrichment factor (EF) and geoaccumulation index (Igeo) indicated anthropogenic sources of accumulation, while, ecological risk assessment (Eix) and potential ecological risk index (PERI) indicated potential hazards for biota due to the accumulation of Pb, Zn and As elements.
Philomina Onyedikachi Peter; Azhar Rashid; Liyuan Hou; François Nkinahamira; Claude Kiki; Qian Sun; Chang-Ping Yu; Anyi Hu. Elemental Contaminants in Surface Sediments from Jiulong River Estuary, China: Pollution Level and Ecotoxicological Risk Assessment. Water 2020, 12, 1640 .
AMA StylePhilomina Onyedikachi Peter, Azhar Rashid, Liyuan Hou, François Nkinahamira, Claude Kiki, Qian Sun, Chang-Ping Yu, Anyi Hu. Elemental Contaminants in Surface Sediments from Jiulong River Estuary, China: Pollution Level and Ecotoxicological Risk Assessment. Water. 2020; 12 (6):1640.
Chicago/Turabian StylePhilomina Onyedikachi Peter; Azhar Rashid; Liyuan Hou; François Nkinahamira; Claude Kiki; Qian Sun; Chang-Ping Yu; Anyi Hu. 2020. "Elemental Contaminants in Surface Sediments from Jiulong River Estuary, China: Pollution Level and Ecotoxicological Risk Assessment." Water 12, no. 6: 1640.
Rivers are a significant reservoir of antibiotic resistance genes ARGs), yet the biogeographic pattern of riverine ARGs and its underlying driving forces remain poorly understood. Here, we used metagenomic approach to investigate the spatio-temporal variation of ARGs in two adjacent sub-watersheds viz. North River (NR) and West River (WR), China. The results demonstrated that Bacitracin (22.8 % of the total ARGs), multidrug (20.7 %), sulfonamide (15.2 %) and tetracycline (10.9 %) were the dominant ARG types. SourceTracker analysis indicated that sewage treatment plants as the main source of ARGs, while animal feces mainly contributed in spreading the ARGs in the upstream of NR. Random forest and network analyses confirmed that NR was under the influence of fecal pollution. PCoA analysis demonstrated that the composition of ARGs changed along with the anthropogenic gradients, while the Raup–Crick null model showed that homogenizing selection mediated by class 1 integron intI1 resulted in stable ARG communities at whole watershed scale. Structural equation models revealed that microbial community, grassland and several non-antibiotic micropollutants may also play certain roles in influencing the distribution of ARGs. Overall, the observed deterministic formation of ARGs in riverine systems calls effective management strategies to mitigate the risks of antibiotic resistance on public health.
Anyi Hu; Hongjie Wang; Jiangwei Li; Sikandar I. Mulla; Quanyi Qiu; Lina Tang; Azhar Rashid; Yang Wu; Qian Sun; Chang-Ping Yu. Homogeneous selection drives antibiotic resistome in two adjacent sub-watersheds, China. Journal of Hazardous Materials 2020, 398, 122820 .
AMA StyleAnyi Hu, Hongjie Wang, Jiangwei Li, Sikandar I. Mulla, Quanyi Qiu, Lina Tang, Azhar Rashid, Yang Wu, Qian Sun, Chang-Ping Yu. Homogeneous selection drives antibiotic resistome in two adjacent sub-watersheds, China. Journal of Hazardous Materials. 2020; 398 ():122820.
Chicago/Turabian StyleAnyi Hu; Hongjie Wang; Jiangwei Li; Sikandar I. Mulla; Quanyi Qiu; Lina Tang; Azhar Rashid; Yang Wu; Qian Sun; Chang-Ping Yu. 2020. "Homogeneous selection drives antibiotic resistome in two adjacent sub-watersheds, China." Journal of Hazardous Materials 398, no. : 122820.
Geographic patterns of bacteria and microeukaryotes have attracted increasing attention. However, mechanisms underlying geographic patterns in the community composition of both microbial groups are still poorly resolved. In particular, knowledge of whether bacterial communities and microeukaryotic communities are subject to the same or different assembly mechanisms is still limited. In this study, we investigated the biogeographic patterns of bacterial and microeukaryotic communities of 23 lakes on the Tibetan Plateau and quantified the relative influence of assembly mechanisms in shaping both microbial communities. Results showed that water salinity was the major driving force in controlling the community structures of bacteria and microeukaryotes. Although bacterial and microeukaryotic communities exhibited similar distance-decay patterns, the bacterial communities were mainly governed by environmental filtering (a niche-related process), whereas microeukaryotic communities were strongly driven by dispersal limitation (a neutral-related process). Furthermore, we found that bacteria exhibited wider niche breadths and higher dispersal ability but lower community stabilities than microeukaryotes. The similar distribution patterns but contrasting assembly mechanisms effecting bacteria and microeukaryotes resulted from the differences in dispersal ability and community stability. Our results highlight the importance of considering organism types in studies of the assembly mechanisms that shape microbial communities in microbial ecology.
Keshao Liu; Yongqin Liu; Anyi Hu; Feng Wang; Yuying Chen; Zhengquan Gu; Sten Anslan; Juzhi Hou. Different community assembly mechanisms underlie similar biogeography of bacteria and microeukaryotes in Tibetan lakes. FEMS Microbiology Ecology 2020, 96, 1 .
AMA StyleKeshao Liu, Yongqin Liu, Anyi Hu, Feng Wang, Yuying Chen, Zhengquan Gu, Sten Anslan, Juzhi Hou. Different community assembly mechanisms underlie similar biogeography of bacteria and microeukaryotes in Tibetan lakes. FEMS Microbiology Ecology. 2020; 96 (6):1.
Chicago/Turabian StyleKeshao Liu; Yongqin Liu; Anyi Hu; Feng Wang; Yuying Chen; Zhengquan Gu; Sten Anslan; Juzhi Hou. 2020. "Different community assembly mechanisms underlie similar biogeography of bacteria and microeukaryotes in Tibetan lakes." FEMS Microbiology Ecology 96, no. 6: 1.
Micropollutants have become of great concern, because of their disrupting effects on the structure and function of microbial communities. However, little is known about the relative importance of trace micropollutants on the aquatic prokaryotic communities as compared to the traditional physico-chemical characteristics, especially at different spatial dimensions. Here, we investigated free-living (FL) and particle-associated (PA) prokaryotic communities in a subtropical water reservoir, China, across seasons at horizontal (surface water) and vertical (depth-profile) scales by using 16S rRNA gene amplicon sequencing. Our results showed that the shared variances of physico-chemicals and micropollutants explained majority of the spatial variations in prokaryotic communities, suggesting a strong joint effect of the two abiotic categories on reservoir prokaryotic communities. Micropollutants appeared to exert strong independent influence on the core sub-communities (i.e., abundant and wide-spread taxa) than on the satellite (i.e., less abundant and narrow-range taxa) counterparts. The pure effect of micropollutants on both core and satellite sub-communities from FL and PA fractions was ~1.5 folds greater than that of physico-chemical factors at the horizontal scale, whereas an opposite effect was observed at the vertical scale. Moreover, eight micropollutants including anti-fungal agents, antibiotics, bisphenol analogues, stimulant and UV-filter were identified as the major disrupting compounds with strong associations with core taxa of typical freshwater prokaryotes. Altogether, we concluded that the ecological disrupting effects of micropollutants on prokaryotic communities may vary along horizontal and vertical dimensions in freshwater ecosystems.
Bob Adyari; Dandan Shen; Shuang Li; Lanping Zhang; Azhar Rashid; Qian Sun; Anyi Hu; Nengwang Chen; Chang-Ping Yu. Strong impact of micropollutants on prokaryotic communities at the horizontal but not vertical scales in a subtropical reservoir, China. Science of The Total Environment 2020, 721, 137767 .
AMA StyleBob Adyari, Dandan Shen, Shuang Li, Lanping Zhang, Azhar Rashid, Qian Sun, Anyi Hu, Nengwang Chen, Chang-Ping Yu. Strong impact of micropollutants on prokaryotic communities at the horizontal but not vertical scales in a subtropical reservoir, China. Science of The Total Environment. 2020; 721 ():137767.
Chicago/Turabian StyleBob Adyari; Dandan Shen; Shuang Li; Lanping Zhang; Azhar Rashid; Qian Sun; Anyi Hu; Nengwang Chen; Chang-Ping Yu. 2020. "Strong impact of micropollutants on prokaryotic communities at the horizontal but not vertical scales in a subtropical reservoir, China." Science of The Total Environment 721, no. : 137767.
Increasing extreme precipitation events (EPEs) can induce biogeochemical disturbances in the coastal lagoon ecosystems. Very little is known about the response of prokaryotic communities to such influences, which are the key components mediating the biogeochemical cycling in lagoons. Here 16S rRNA gene amplicon sequencing and high-through quantitative PCR (HT-qPCR) were employed to investigate the distribution of prokaryotic communities and fecal indicator genes in the surface waters of Yundang lagoon, Xiamen, China during EPEs, respectively. Prokaryotic communities from rainwaters, influents (IFs) and effluents (EFs) from a nearby wastewater treatment plant were also characterised. The results indicated a significant variation in the composition of lagoon prokaryotic communities compared with rainwaters, IFs and EFs. Multivariate and phylogenetic signal analyses revealed that environmental filtering, mainly controlled by salinity, was the major ecological process responsible for the temporal succession of lagoon prokaryotic communities during EPEs. Moreover, the pollution indicator taxa (based on amplicon sequencing) and fecal indicator genes (based on HT-qPCR) demonstrated that EPEs may induce sewage overflows and fecal pollution (mainly from humans and dogs), resulting in an increase in the relative abundance of pollution indicator taxa and genes in Yundang lagoon. Network analysis illustrated that the number of network edges and keystone species decreased along the sampling times, implying that EPEs-induced disturbances may affect prokaryotic species associations. Taken together, this study provides an enhanced understanding of the responses of lagoon prokaryotic communities to EPEs-induced disturbances.
Hongjie Wang; Xiaoyong Yang; Qingfu Chen; Jian-Qiang Su; Sikandar I. Mulla; Azhar Rashid; Anyi Hu; Chang-Ping Yu. Response of prokaryotic communities to extreme precipitation events in an urban coastal lagoon: A case study of Yundang lagoon, China. Science of The Total Environment 2019, 706, 135937 .
AMA StyleHongjie Wang, Xiaoyong Yang, Qingfu Chen, Jian-Qiang Su, Sikandar I. Mulla, Azhar Rashid, Anyi Hu, Chang-Ping Yu. Response of prokaryotic communities to extreme precipitation events in an urban coastal lagoon: A case study of Yundang lagoon, China. Science of The Total Environment. 2019; 706 ():135937.
Chicago/Turabian StyleHongjie Wang; Xiaoyong Yang; Qingfu Chen; Jian-Qiang Su; Sikandar I. Mulla; Azhar Rashid; Anyi Hu; Chang-Ping Yu. 2019. "Response of prokaryotic communities to extreme precipitation events in an urban coastal lagoon: A case study of Yundang lagoon, China." Science of The Total Environment 706, no. : 135937.
The main goal of the analysis of microbial ecology is to understand the relationship between Earth’s microbial community and their functions in the environment. This paper presents a proof-of-concept research to develop a bioclimatic modeling approach that leverages artificial intelligence techniques to identify the microbial species in a river as a function of physicochemical parameters. Feature reduction and selection are both utilized in the data preprocessing owing to the scarce of available data points collected and missing values of physicochemical attributes from a river in Southeast China. A bio-inspired metaheuristic optimized machine learner, which supports the adjustment to the multiple-output prediction form, is used in bioclimatic modeling. The accuracy of prediction and applicability of the model can help microbiologists and ecologists in quantifying the predicted microbial species for further experimental planning with minimal expenditure, which is become one of the most serious issues when facing dramatic changes of environmental conditions caused by global warming. This work demonstrates a neoteric approach for potential use in predicting preliminary microbial structures in the environment.
Jui-Sheng Chou; Chang-Ping Yu; Dinh-Nhat Truong; Billy Susilo; Anyi Hu; Qian Sun. Predicting Microbial Species in a River Based on Physicochemical Properties by Bio-Inspired Metaheuristic Optimized Machine Learning. Sustainability 2019, 11, 6889 .
AMA StyleJui-Sheng Chou, Chang-Ping Yu, Dinh-Nhat Truong, Billy Susilo, Anyi Hu, Qian Sun. Predicting Microbial Species in a River Based on Physicochemical Properties by Bio-Inspired Metaheuristic Optimized Machine Learning. Sustainability. 2019; 11 (24):6889.
Chicago/Turabian StyleJui-Sheng Chou; Chang-Ping Yu; Dinh-Nhat Truong; Billy Susilo; Anyi Hu; Qian Sun. 2019. "Predicting Microbial Species in a River Based on Physicochemical Properties by Bio-Inspired Metaheuristic Optimized Machine Learning." Sustainability 11, no. 24: 6889.
Coastal sands harbor diverse microbial assemblages that play a critical role in the biogeochemical cycling of beach ecosystems. However, little is known about the relative importance of the different ecological processes underlying the assembly of communities of sand microbiota. Here, we employed 16S rDNA amplicon sequencing to investigate the sand microbiota of two coastal beaches, in southern China. The results showed that sand microbial assemblages at intertidal and supratidal zones exhibited contrasting compositions that can be attributed to environmental filtering by electric conductivity. A consistent pattern of habitat generalists and specialists of sand microbiota was observed among different beach zones. Null and neutral model analyses indicated that the environmental filtering was mainly responsible for supratidal microbial communities, while the neutral processes could partially influence the assembly of intertidal communities. Moreover, environmental filtering was found to shape the habitat specialists, while random dispersal played a major role in shaping generalists. The neutral model analysis revealed that the habitat generalists exceeding the neutral prediction harbored a relatively higher proportion of microbial taxa than the specialist counterparts. An opposite pattern was observed for taxa falling below the neutral prediction. Collectively, these findings offer a novel insight into the assembly mechanisms of coastal sand microbiota.
Anyi Hu; Hongjie Wang; Meixian Cao; Azhar Rashid; Mingfeng Li; Chang-Ping Yu. Environmental Filtering Drives the Assembly of Habitat Generalists and Specialists in the Coastal Sand Microbial Communities of Southern China. Microorganisms 2019, 7, 598 .
AMA StyleAnyi Hu, Hongjie Wang, Meixian Cao, Azhar Rashid, Mingfeng Li, Chang-Ping Yu. Environmental Filtering Drives the Assembly of Habitat Generalists and Specialists in the Coastal Sand Microbial Communities of Southern China. Microorganisms. 2019; 7 (12):598.
Chicago/Turabian StyleAnyi Hu; Hongjie Wang; Meixian Cao; Azhar Rashid; Mingfeng Li; Chang-Ping Yu. 2019. "Environmental Filtering Drives the Assembly of Habitat Generalists and Specialists in the Coastal Sand Microbial Communities of Southern China." Microorganisms 7, no. 12: 598.
Wastewater treatment plants (WWTPs) rely mainly on the microbial assemblages to contribute significantly for the removal of organic pollutants and nutrients. However, limited information is available on the ecological driving forces underlying the turnover of prokaryotic communities across wastewater treatment processes (i.e., from influents (IFs) and effluents (EFs)) within WWTPs. Here, we used a combination of the 16S rRNA gene amplicon sequencing and a quantitative ecological null model analysis to explore the ecological processes governing the turnover of the prokaryotic communities and the dominant taxonomic taxa across wastewater treatment processes of five full-scale WWTPs in China. Our results indicated that a significant variation in the composition of prokaryotic communities and the dominant taxa between IFs and EFs. The analysis of the environmental sources of indicator OTUs showed that a relatively lower abundance of the sludge/sewage and human guts associated OTUs in EFs than in IFs. Ecological null models revealed that among the ecological processes, deterministic processes were dominant in controlling the turnover of the overall communities from IFs to EFs, whereas the relative importance of deterministic processes varied among the dominant taxa (i.e., Bacteroidetes > Proteobacteria > Gammaproteobacteria > Firmicutes > Betaproteobacteria). However, the assembly of IF and EF communities was influenced mainly by the deterministic and stochastic processes, respectively. In addition, our results indicated that EF communities have a higher phylogenetic diversity than those of the IF communities, but the abundance of prokaryotic 16S rRNA genes was lower in EFs than in IFs. Overall, our study provides a novel insight of the assembly mechanisms underlying the turnover of prokaryotic communities during wastewater treatment processes.
Liyuan Hou; Sikandar I. Mulla; Juan Pablo Niño-Garcia; Daliang Ning; Azhar Rashid; Anyi Hu; Chang-Ping Yu. Deterministic and stochastic processes driving the shift in the prokaryotic community composition in wastewater treatment plants of a coastal Chinese city. Applied Microbiology and Biotechnology 2019, 103, 9155 -9168.
AMA StyleLiyuan Hou, Sikandar I. Mulla, Juan Pablo Niño-Garcia, Daliang Ning, Azhar Rashid, Anyi Hu, Chang-Ping Yu. Deterministic and stochastic processes driving the shift in the prokaryotic community composition in wastewater treatment plants of a coastal Chinese city. Applied Microbiology and Biotechnology. 2019; 103 (21-22):9155-9168.
Chicago/Turabian StyleLiyuan Hou; Sikandar I. Mulla; Juan Pablo Niño-Garcia; Daliang Ning; Azhar Rashid; Anyi Hu; Chang-Ping Yu. 2019. "Deterministic and stochastic processes driving the shift in the prokaryotic community composition in wastewater treatment plants of a coastal Chinese city." Applied Microbiology and Biotechnology 103, no. 21-22: 9155-9168.
The free-living (FL) and particle-attached (PA) bacteria have different dispersal potentials and ecological roles in aquatic ecosystems. However, our knowledge of their diversity and regional biogeographic patterns in high-altitude alpine lakes is limited. In this study, to investigate the diversity and geographic patterns of the FL and PA bacterial communities and to quantify the relative influence of environmental filtering and dispersal limitation in shaping the FL and PA bacterial communities, we collected surface water samples from 26 lakes over 1000 kilometers on the Tibetan Plateau. The majority of sequences exclusively in the FL bacteria were Actinobacteria (29.4%), Proteobacteria (27.7%) and Bacteroidetes (21.6%), while sequences exclusively in the PA bacteria were dominated by Proteobacteria (57.9%). The α-diversity indices, including Shannon index and Pielou's evenness, were significantly lower in the FL bacteria than that in PA bacteria. The surrounding soils as an important potential source contributed more to the diversity of the PA bacteria than the FL bacteria. Both of the FL and PA bacterial communities exhibited a significant regional distance-decay pattern. Environmental filtering and dispersal limitation were significantly related to the spatial variation of the FL and PA bacterial communities, whereas the environmental filtering/dispersal limitation effect ratio was higher in the FL bacterial communities. Our study is the first to describe the regional-scale spatial variability and to identify the factors that drive regional variability of the FL and PA bacterial communities in Tibetan lakes.
Keshao Liu; Juzhi Hou; Yongqin Liu; Anyi Hu; Mingda Wang; Feng Wang; Yuying Chen; Zhengquan Gu. Biogeography of the free-living and particle-attached bacteria in Tibetan lakes. FEMS Microbiology Ecology 2019, 95, 1 .
AMA StyleKeshao Liu, Juzhi Hou, Yongqin Liu, Anyi Hu, Mingda Wang, Feng Wang, Yuying Chen, Zhengquan Gu. Biogeography of the free-living and particle-attached bacteria in Tibetan lakes. FEMS Microbiology Ecology. 2019; 95 (7):1.
Chicago/Turabian StyleKeshao Liu; Juzhi Hou; Yongqin Liu; Anyi Hu; Mingda Wang; Feng Wang; Yuying Chen; Zhengquan Gu. 2019. "Biogeography of the free-living and particle-attached bacteria in Tibetan lakes." FEMS Microbiology Ecology 95, no. 7: 1.
Food waste (FW) contributes to a great proportion in the municipal solid waste and is generated during disposal from different life cycles of food processing and consumption. FW treatment is a big challenge and there is an urgent need to develop a suitable treatment technology. The microbial fuel cell (MFC) is a promising bioelectrochemical technology using bacteria as the catalyst, which has been developed to effectively generate bioelectricity from diverse organic wastes. The present study investigated the treatment of food waste collected from a Chinese canteen using MFCs under different conditions. It was observed that the highest closed circuit voltage and maximum power density obtained were 775 ± 21 mV and 422 mW m-2, respectively, when using food waste with nutrient medium as the anolyte and permanganate as the catholyte. Under this condition, biodegradation processes in the MFCs could achieve 69 ± 18 % COD, 88 ± 5 % carbohydrate, 76 ± 9 % protein, 65 ± 8 % TOC and 71 ± 8 % total nitrogen removal. Microbial community analysis using 16S rRNA gene high-throughput sequencing showed Firmicutes, Bacteroidetes and Proteobacteria were the dominant phyla and Geobacter was the most abundant genus. Therefore, our microbial community results suggested that the mixture of exoelectrogenic and fermentative bacteria have a pronounced effect on MFCs system treating FW while affecting on organic degradation and energy production. A power management system was used to demonstrate that electricity from FW-fed MFCs can be successfully harvested to provide intermittent electricity to loads. Overall, this study demonstrated the potential of using MFCs for food waste treatment to achieve electricity production and waste reduction.
Bahareh Asefi; Shiue-Lin Li; Henry A. Moreno; Viviana Sanchez-Torres; Anyi Hu; Jiangwei Li; Chang-Ping Yu. Characterization of electricity production and microbial community of food waste-fed microbial fuel cells. Process Safety and Environmental Protection 2019, 125, 83 -91.
AMA StyleBahareh Asefi, Shiue-Lin Li, Henry A. Moreno, Viviana Sanchez-Torres, Anyi Hu, Jiangwei Li, Chang-Ping Yu. Characterization of electricity production and microbial community of food waste-fed microbial fuel cells. Process Safety and Environmental Protection. 2019; 125 ():83-91.
Chicago/Turabian StyleBahareh Asefi; Shiue-Lin Li; Henry A. Moreno; Viviana Sanchez-Torres; Anyi Hu; Jiangwei Li; Chang-Ping Yu. 2019. "Characterization of electricity production and microbial community of food waste-fed microbial fuel cells." Process Safety and Environmental Protection 125, no. : 83-91.
The urban water ecosystems, such as the landscape ponds are commonly considered under the influence of anthropogenic disturbances, which can lead to the deterioration of the water quality. The prokaryotic communities are considered as one of the best indicators of the water quality. However, there are significant gaps in understanding the ecological processes that shape the composition and function of prokaryotic communities in the urban water ecosystems. Here, we investigated the biogeographic distribution of prokaryotic assemblages in water environments including landscape ponds, drinking water reservoirs, influents (IFs) and effluents (EFs) of wastewater treatment plants of a coastal city (Xiamen), China, by using 16S rDNA amplicon sequencing. Our results indicated that the ponds had higher α-diversity of prokaryotic communities than those in the reservoirs, while there were significant variations in the community compositions among ponds, reservoirs, IFs and EFs. Moreover, ponds harbored a significantly higher proportion of sewage- and fecal-indicator taxa than those in the reservoirs, suggesting the occurrence of exogenous pollution in the urban ponds. Null model analysis revealed that dispersal limitation was the main ecological processes resulting in the divergence of prokaryotic community compositions between ponds and other environments, while dispersal limitation and variable selection played an essential role in the formation of unique prokaryotic assemblages in the reservoirs. Function predication analysis demonstrated that the ponds shared more similar functional profiles with IFs or EFs (e.g., chemoheterotrophy, fermentation, chlorate reducers, nitrate reduction and respiration) than the reservoirs, whereas dominance of photoautotrophy was observed in the reservoirs. Overall, this study provides a profound insight of the ecological mechanisms underlying the responses of prokaryotic communities in the urban landscape ponds to the anthropogenic disturbances.
Anyi Hu; Shuang Li; Lanping Zhang; Hongjie Wang; Jun Yang; Zhuanxi Luo; Azhar Rashid; Shaoqing Chen; Weixiong Huang; Chang-Ping Yu. Prokaryotic footprints in urban water ecosystems: A case study of urban landscape ponds in a coastal city, China. Environmental Pollution 2018, 242, 1729 -1739.
AMA StyleAnyi Hu, Shuang Li, Lanping Zhang, Hongjie Wang, Jun Yang, Zhuanxi Luo, Azhar Rashid, Shaoqing Chen, Weixiong Huang, Chang-Ping Yu. Prokaryotic footprints in urban water ecosystems: A case study of urban landscape ponds in a coastal city, China. Environmental Pollution. 2018; 242 ():1729-1739.
Chicago/Turabian StyleAnyi Hu; Shuang Li; Lanping Zhang; Hongjie Wang; Jun Yang; Zhuanxi Luo; Azhar Rashid; Shaoqing Chen; Weixiong Huang; Chang-Ping Yu. 2018. "Prokaryotic footprints in urban water ecosystems: A case study of urban landscape ponds in a coastal city, China." Environmental Pollution 242, no. : 1729-1739.
Although the health of rivers is threatened by multiple anthropogenic stressors with increasing frequency, it remains an open question how riverine microbial communities respond to emerging micropollutants. Here, by using 16S rDNA amplicon sequencing of 60 water samples collected during different hydrological seasons, we investigated the spatio‐temporal variation and the co‐occurrence patterns of microbial communities in the anthropogenically impacted Jiulong River in China. The results indicated that the riverine microbial co‐occurrence network had a nonrandom, modular structure, which was mainly shaped by the taxonomic relatedness of co‐occurring species. Fecal indicator bacteria may survive for prolonged periods of time in river water, but they formed an independent module which had fewer interactions with typical freshwater bacteria. Multivariate analysis demonstrated that nutrients and micropollutants [i.e., pharmaceuticals and personal care products (PPCPs)] exerted combined effects in shaping α‐ and β‐diversity of riverine microbial communities. Remarkably, we showed that a hitherto unrecognized disruptive effect of PPCPs on the abundance variations of central species and module communities was stronger than the influence of physicochemical factors, suggesting the key role played by micropollutants for the microbial co‐occurrence relationships in lotic ecosystems. Overall, our findings provide novel insights into community assembly in aquatic environments experiencing anthropogenic stresses.
Anyi Hu; Feng Ju; Liyuan Hou; Jiangwei Li; Xiaoyong Yang; Hongjie Wang; Sikandar I. Mulla; Qian Sun; Helmut Bürgmann; Chang-Ping Yu. Strong impact of anthropogenic contamination on the co-occurrence patterns of a riverine microbial community. Environmental Microbiology 2017, 19, 4993 -5009.
AMA StyleAnyi Hu, Feng Ju, Liyuan Hou, Jiangwei Li, Xiaoyong Yang, Hongjie Wang, Sikandar I. Mulla, Qian Sun, Helmut Bürgmann, Chang-Ping Yu. Strong impact of anthropogenic contamination on the co-occurrence patterns of a riverine microbial community. Environmental Microbiology. 2017; 19 (12):4993-5009.
Chicago/Turabian StyleAnyi Hu; Feng Ju; Liyuan Hou; Jiangwei Li; Xiaoyong Yang; Hongjie Wang; Sikandar I. Mulla; Qian Sun; Helmut Bürgmann; Chang-Ping Yu. 2017. "Strong impact of anthropogenic contamination on the co-occurrence patterns of a riverine microbial community." Environmental Microbiology 19, no. 12: 4993-5009.
The response of freshwater bacterial community to anthropogenic disturbance has been well documented, yet the studies of freshwater archaeal community are rare, especially in lotic environments. Here, we investigated planktonic and benthic archaeal communities in a human-perturbed watershed (Jiulong River Watershed, JRW) of southeast China by using Illumina 16S ribosomal RNA gene amplicon sequencing. The results of taxonomic assignments indicated that SAGMGC-1, Methanobacteriaceae, Methanospirillaceae, and Methanoregulaceae were the four most abundant families in surface waters, accounting for 12.65, 23.21, 18.58 and 10.97 % of planktonic communities, whereas Nitrososphaeraceae and Miscellaneous Crenarchaeotic Group occupied more than 49 % of benthic communities. The compositions of archaeal communities and populations in waters and sediments were significantly different from each other. Remarkably, the detection frequencies of families Methanobacteriaceae and Methanospirillaceae, and genera Methanobrevibacter and Methanosphaera in planktonic communities correlated strongly with bacterial fecal indicator, suggesting some parts of methanogenic Archaea may come from fecal contamination. Because soluble reactive phosphorus (SRP) and the ratio of dissolved inorganic nitrogen to SRP instead of nitrogen nutrients showed significant correlation with several planktonic Nitrosopumilus- and Nitrosotalea-like OTUs, Thaumarchaeota may play an unexplored role in biogeochemical cycling of river phosphorus. Multivariate statistical analyses revealed that the variation of α-diversity of planktonic archaeal community was best explained by water temperature, whereas nutrient concentrations and stoichiometry were the significant drivers of β-diversity of planktonic and benthic communities. Taken together, these results demonstrate that the structure of archaeal communities in the JRW is sensitive to anthropogenic disturbances caused by riparian human activities.
Anyi Hu; Hongjie Wang; Jiangwei Li; Jing Liu; Nengwang Chen; Chang-Ping Yu. Archaeal community in a human-disturbed watershed in southeast China: diversity, distribution, and responses to environmental changes. Applied Microbiology and Biotechnology 2016, 100, 4685 -4698.
AMA StyleAnyi Hu, Hongjie Wang, Jiangwei Li, Jing Liu, Nengwang Chen, Chang-Ping Yu. Archaeal community in a human-disturbed watershed in southeast China: diversity, distribution, and responses to environmental changes. Applied Microbiology and Biotechnology. 2016; 100 (10):4685-4698.
Chicago/Turabian StyleAnyi Hu; Hongjie Wang; Jiangwei Li; Jing Liu; Nengwang Chen; Chang-Ping Yu. 2016. "Archaeal community in a human-disturbed watershed in southeast China: diversity, distribution, and responses to environmental changes." Applied Microbiology and Biotechnology 100, no. 10: 4685-4698.