To promote the application of strain FY-07 in oilfield development, experiments were performed on the mechanism of microscopic profile control using an etched glass micromodel. The physical simulation experiments were applied to evaluate the plugging ability and oil displacement performance. The results showed that the biopolymers were preferentially generated and existed in large pores that can improve the swept volume and oil recovery. The plugging rate of FY-07 for different permeability cores was 67–78%, which indicates FY-07 exhibited good plugging capacity. The EOR value of FY-07 was 10.54%, which demonstrated that FY-07 has great potential to enhance oil recovery.

To study the feasibility of polymer-producing bacteria Enterobacter cloacae (E. cloacae) FY-07 and surfactant-producing bacteria Pseudomonas aeruginosa WJ-1 combined profile control and flooding, the compatibility of FY-07 and WJ-1 was evaluated using laboratory experiments. The results showed that the growth and metabolism of WJ-1 was not significantly affected by the FY-07 in the degradation medium, and the surface tension of fermentation broth was reduced from 70 mN/m to 30 mN/m. FY-07 enhanced the degradation of WJ-1, increasing the ratio of C14- to C15+ from 0.37 to 0.67. The core-flooding experiments indicated the oil recovery of 17.4% when both FY-07 and WJ-1 were injected into the system, as against to 10.4% and 7.9% for FY-07 and WJ-1, respectively, when injected alone. The results demonstrate a good compatibility between the FY-07 and WJ-1 strains and highlight the application potential of stain FY-07 and strain WJ-1 compound flooding for enhancing the oil recovery in heterogeneous reservoirs.