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In mammalian cells, two cellular organelles, mitochondria and peroxisomes, share the ability to degrade fatty acid chains. Although each organelle harbors its own fatty acid β-oxidation pathway, a distinct mitochondrial system feeds the oxidative phosphorylation pathway for ATP synthesis. At the same time, the peroxisomal β-oxidation pathway participates in cellular thermogenesis. A scientific milestone in 1965 helped discover the hepatomegaly effect in rat liver by clofibrate, subsequently identified as a peroxisome proliferator in rodents and an activator of the peroxisomal fatty acid β-oxidation pathway. These peroxisome proliferators were later identified as activating ligands of Peroxisome Proliferator-Activated Receptor α (PPARα), cloned in 1990. The ligand-activated heterodimer PPARα/RXRα recognizes a DNA sequence, called PPRE (Peroxisome Proliferator Response Element), corresponding to two half-consensus hexanucleotide motifs, AGGTCA, separated by one nucleotide. Accordingly, the assembled complex containing PPRE/PPARα/RXRα/ligands/Coregulators controls the expression of the genes involved in liver peroxisomal fatty acid β-oxidation. This review mobilizes a considerable number of findings that discuss miscellaneous axes, covering the detailed expression pattern of PPARα in species and tissues, the lessons from several PPARα KO mouse models and the modulation of PPARα function by dietary micronutrients.
Mounia Tahri-Joutey; Pierre Andreoletti; Sailesh Surapureddi; Boubker Nasser; Mustapha Cherkaoui-Malki; Norbert Latruffe. Mechanisms Mediating the Regulation of Peroxisomal Fatty Acid Beta-Oxidation by PPARα. International Journal of Molecular Sciences 2021, 22, 8969 .
AMA StyleMounia Tahri-Joutey, Pierre Andreoletti, Sailesh Surapureddi, Boubker Nasser, Mustapha Cherkaoui-Malki, Norbert Latruffe. Mechanisms Mediating the Regulation of Peroxisomal Fatty Acid Beta-Oxidation by PPARα. International Journal of Molecular Sciences. 2021; 22 (16):8969.
Chicago/Turabian StyleMounia Tahri-Joutey; Pierre Andreoletti; Sailesh Surapureddi; Boubker Nasser; Mustapha Cherkaoui-Malki; Norbert Latruffe. 2021. "Mechanisms Mediating the Regulation of Peroxisomal Fatty Acid Beta-Oxidation by PPARα." International Journal of Molecular Sciences 22, no. 16: 8969.
The Mediterranean diet is a central element of a healthy lifestyle, where polyphenols play a key role due to their anti-oxidant properties, and for some of them, as nutripharmacological compounds capable of preventing a number of diseases, including cancer. Due to the high prevalence of intestinal cancer (ranking second in causing morbidity and mortality), this review is focused on the beneficial effects of selected dietary phytophenols, largely present in Mediterranean cooking: apigenin, curcumin, epigallocatechin gallate, quercetin-rutine, and resveratrol. The role of the Mediterranean diet in the prevention of colorectal cancer and future perspectives are discussed in terms of food polyphenol content, the effectiveness, the plasma level, and the importance of other factors, such as the polyphenol metabolites and the influence of the microbiome. Perspectives are discussed in terms of microbiome-dependency of the brain-second brain axis. The emergence of polyphenol formulations may strengthen the efficiency of the Mediterranean diet in the prevention of cancer.
Aline Yammine; Amira Namsi; Dominique Vervandier-Fasseur; John Mackrill; Gérard Lizard; Norbert Latruffe. Polyphenols of the Mediterranean Diet and Their Metabolites in the Prevention of Colorectal Cancer. Molecules 2021, 26, 3483 .
AMA StyleAline Yammine, Amira Namsi, Dominique Vervandier-Fasseur, John Mackrill, Gérard Lizard, Norbert Latruffe. Polyphenols of the Mediterranean Diet and Their Metabolites in the Prevention of Colorectal Cancer. Molecules. 2021; 26 (12):3483.
Chicago/Turabian StyleAline Yammine; Amira Namsi; Dominique Vervandier-Fasseur; John Mackrill; Gérard Lizard; Norbert Latruffe. 2021. "Polyphenols of the Mediterranean Diet and Their Metabolites in the Prevention of Colorectal Cancer." Molecules 26, no. 12: 3483.
The Mediterranean diet is associated with health benefits due to bioactive compounds such as polyphenols. The biological activities of three polyphenols (quercetin (QCT), resveratrol (RSV), apigenin (API)) were evaluated in mouse neuronal N2a cells in the presence of 7-ketocholesterol (7KC), a major cholesterol oxidation product increased in patients with age-related diseases, including neurodegenerative disorders. In N2a cells, 7KC (50 µM; 48 h) induces cytotoxic effects characterized by an induction of cell death. When associated with RSV, QCT and API (3.125; 6.25 µM), 7KC-induced toxicity was reduced. The ability of QCT, RSV and API to prevent 7KC-induced oxidative stress was characterized by a decrease in reactive oxygen species (ROS) production in whole cells and at the mitochondrial level; by an attenuation of the increase in the level and activity of catalase; by attenuating the decrease in the expression, level and activity of glutathione peroxidase 1 (GPx1); by normalizing the expression, level and activity of superoxide dismutases 1 and 2 (SOD1, SOD2); and by reducing the decrease in the expression of nuclear erythroid 2-like factor 2 (Nrf2) which regulates antioxidant genes. QCT, RSV and API also prevented mitochondrial dysfunction in 7KC-treated cells by counteracting the loss of mitochondrial membrane potential (ΨΔm) and attenuating the decreased gene expression and/or protein level of AMP-activated protein kinase α (AMPKα), sirtuin 1 (SIRT1) and peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) implicated in mitochondrial biogenesis. At the peroxisomal level, QCT, RSV and API prevented the impact of 7KC by counteracting the decrease in ATP binding cassette subfamily D member (ABCD)3 (a peroxisomal mass marker) at the protein and mRNA levels, as well as the decreased expresssion of genes associated with peroxisomal biogenesis (Pex13, Pex14) and peroxisomal β-oxidation (Abcd1, Acox1, Mfp2, Thiolase A). The 7KC-induced decrease in ABCD1 and multifunctional enzyme type 2 (MFP2), two proteins involved in peroxisomal β-oxidation, was also attenuated by RSV, QCT and API. 7KC-induced cell death, which has characteristics of apoptosis (cells with fragmented and/or condensed nuclei; cleaved caspase-3; Poly(ADP-ribose) polymerase (PARP) fragmentation) and autophagy (cells with monodansyl cadaverine positive vacuoles; activation of microtubule associated protein 1 light chain 3–I (LC3-I) to LC3-II, was also strongly attenuated by RSV, QCT and API. Thus, in N2a cells, 7KC induces a mode of cell death by oxiapoptophagy, including criteria of OXIdative stress, APOPTOsis and autoPHAGY, associated with mitochondrial and peroxisomal dysfunction, which is counteracted by RSV, QCT, and API reinforcing the interest for these polyphenols in prevention of diseases associated with increased 7KC levels.
Aline Yammine; Amira Zarrouk; Thomas Nury; Anne Vejux; Norbert Latruffe; Dominique Vervandier-Fasseur; Mohammad Samadi; John J. Mackrill; Hélène Greige-Gerges; Lizette Auezova; Gérard Lizard. Prevention by Dietary Polyphenols (Resveratrol, Quercetin, Apigenin) Against 7-Ketocholesterol-Induced Oxiapoptophagy in Neuronal N2a Cells: Potential Interest for the Treatment of Neurodegenerative and Age-Related Diseases. Cells 2020, 9, 2346 .
AMA StyleAline Yammine, Amira Zarrouk, Thomas Nury, Anne Vejux, Norbert Latruffe, Dominique Vervandier-Fasseur, Mohammad Samadi, John J. Mackrill, Hélène Greige-Gerges, Lizette Auezova, Gérard Lizard. Prevention by Dietary Polyphenols (Resveratrol, Quercetin, Apigenin) Against 7-Ketocholesterol-Induced Oxiapoptophagy in Neuronal N2a Cells: Potential Interest for the Treatment of Neurodegenerative and Age-Related Diseases. Cells. 2020; 9 (11):2346.
Chicago/Turabian StyleAline Yammine; Amira Zarrouk; Thomas Nury; Anne Vejux; Norbert Latruffe; Dominique Vervandier-Fasseur; Mohammad Samadi; John J. Mackrill; Hélène Greige-Gerges; Lizette Auezova; Gérard Lizard. 2020. "Prevention by Dietary Polyphenols (Resveratrol, Quercetin, Apigenin) Against 7-Ketocholesterol-Induced Oxiapoptophagy in Neuronal N2a Cells: Potential Interest for the Treatment of Neurodegenerative and Age-Related Diseases." Cells 9, no. 11: 2346.
The health benefits of moderate wine consumption have been extensively studied during the last few decades. Some studies have demonstrated protective associations between moderate drinking and several diseases including oral cavity cancer (OCC). However, due to the various adverse effects related to ethanol content, the recommendation of moderate wine consumption has been controversial. The polyphenolic components of wine contribute to its beneficial effects with different biological pathways, including antioxidant, lipid regulating and anti-inflammatory effects. On the other hand, in the oral cavity, ethanol is oxidized to form acetaldehyde, a metabolite with genotoxic properties. This review is a critical compilation of both the beneficial and the detrimental effects of wine consumption on OCC.
Paula Silva; Norbert Latruffe; Giovanni De Gaetano. Wine Consumption and Oral Cavity Cancer: Friend or Foe, Two Faces of Janus. Molecules 2020, 25, 2569 .
AMA StylePaula Silva, Norbert Latruffe, Giovanni De Gaetano. Wine Consumption and Oral Cavity Cancer: Friend or Foe, Two Faces of Janus. Molecules. 2020; 25 (11):2569.
Chicago/Turabian StylePaula Silva; Norbert Latruffe; Giovanni De Gaetano. 2020. "Wine Consumption and Oral Cavity Cancer: Friend or Foe, Two Faces of Janus." Molecules 25, no. 11: 2569.
The brain, which is a cholesterol-rich organ, can be subject to oxidative stress in a variety of pathophysiological conditions, age-related diseases and some rare pathologies. This can lead to the formation of 7-ketocholesterol (7KC), a toxic derivative of cholesterol mainly produced by auto-oxidation. So, preventing the neuronal toxicity of 7KC is an important issue to avoid brain damage. As there are numerous data in favor of the prevention of neurodegeneration by the Mediterranean diet, this study aimed to evaluate the potential of a series of polyphenols (resveratrol, RSV; quercetin, QCT; and apigenin, API) as well as ω3 and ω9 unsaturated fatty acids (α-linolenic acid, ALA; eicosapentaenoic acid, EPA; docosahexaenoic acid, DHA, and oleic acid, OA) widely present in this diet, to prevent 7KC (50 µM)-induced dysfunction of N2a neuronal cells. When polyphenols and fatty acids were used at non-toxic concentrations (polyphenols: ≤6.25 µM; fatty acids: ≤25 µM) as defined by the fluorescein diacetate assay, they greatly reduce 7KC-induced toxicity. The cytoprotective effects observed with polyphenols and fatty acids were comparable to those of α-tocopherol (400 µM) used as a reference. These polyphenols and fatty acids attenuate the overproduction of reactive oxygen species and the 7KC-induced drop in mitochondrial transmembrane potential (ΔΨm) measured by flow cytometry after dihydroethidium and DiOC6(3) staining, respectively. Moreover, the studied polyphenols and fatty acids reduced plasma membrane permeability considered as a criterion for cell death measured by flow cytometry after propidium iodide staining. Our data show that polyphenols (RSV, QCT and API) as well as ω3 and ω9 unsaturated fatty acids (ALA, EPA, DHA and OA) are potent cytoprotective agents against 7KC-induced neurotoxicity in N2a cells. Their cytoprotective effects could partly explain the benefits of the Mediterranean diet on human health, particularly in the prevention of neurodegenerative diseases.
Aline Yammine; Thomas Nury; Anne Vejux; Norbert Latruffe; Dominique Vervandier-Fasseur; Mohammad Samadi; Hélène Greige-Gerges; Lizette Auezova; Gérard Lizard. Prevention of 7-Ketocholesterol-Induced Overproduction of Reactive Oxygen Species, Mitochondrial Dysfunction and Cell Death with Major Nutrients (Polyphenols, ω3 and ω9 Unsaturated Fatty Acids) of the Mediterranean Diet on N2a Neuronal Cells. Molecules 2020, 25, 2296 .
AMA StyleAline Yammine, Thomas Nury, Anne Vejux, Norbert Latruffe, Dominique Vervandier-Fasseur, Mohammad Samadi, Hélène Greige-Gerges, Lizette Auezova, Gérard Lizard. Prevention of 7-Ketocholesterol-Induced Overproduction of Reactive Oxygen Species, Mitochondrial Dysfunction and Cell Death with Major Nutrients (Polyphenols, ω3 and ω9 Unsaturated Fatty Acids) of the Mediterranean Diet on N2a Neuronal Cells. Molecules. 2020; 25 (10):2296.
Chicago/Turabian StyleAline Yammine; Thomas Nury; Anne Vejux; Norbert Latruffe; Dominique Vervandier-Fasseur; Mohammad Samadi; Hélène Greige-Gerges; Lizette Auezova; Gérard Lizard. 2020. "Prevention of 7-Ketocholesterol-Induced Overproduction of Reactive Oxygen Species, Mitochondrial Dysfunction and Cell Death with Major Nutrients (Polyphenols, ω3 and ω9 Unsaturated Fatty Acids) of the Mediterranean Diet on N2a Neuronal Cells." Molecules 25, no. 10: 2296.
Several series of natural polyphenols are described for their biological and therapeutic potential. Natural stilbenoid polyphenols, such as trans-resveratrol, pterostilbene and piceatannol are well-known for their numerous biological activities. However, their moderate bio-availabilities, especially for trans-resveratrol, prompted numerous research groups to investigate innovative and relevant synthetic resveratrol derivatives. This review is focused on isosteric resveratrol analogs aza-stilbenes and azo-stilbenes in which the C=C bond between both aromatic rings was replaced with C=N or N=N bonds, respectively. In each series, synthetic ways will be displayed, and structural sights will be highlighted and compared with those of resveratrol. The biological activities of some of these molecules will be presented as well as their potential therapeutic applications. In some cases, structure-activity relationships will be discussed.
Gérard Lizard; Norbert Latruffe; Dominique Vervandier-Fasseur. Aza- and Azo-Stilbenes: Bio-Isosteric Analogs of Resveratrol. Molecules 2020, 25, 605 .
AMA StyleGérard Lizard, Norbert Latruffe, Dominique Vervandier-Fasseur. Aza- and Azo-Stilbenes: Bio-Isosteric Analogs of Resveratrol. Molecules. 2020; 25 (3):605.
Chicago/Turabian StyleGérard Lizard; Norbert Latruffe; Dominique Vervandier-Fasseur. 2020. "Aza- and Azo-Stilbenes: Bio-Isosteric Analogs of Resveratrol." Molecules 25, no. 3: 605.
This paper reports that the human peroxisomal 3-ketoacyl-CoA thiolase expression shows three transcripts: Tr1 (1705 bp), Tr2 (1375 bp) and Tr3 (1782 bp). Their highest expression is observed in the human liver and at a lesser extent in hepatic-derived HepG2 cells. The intestine and blood and endothelial cells show lower expression. The lowest expression is found in adipocytes. The transcript Tr3 appears to be the most abundant. So far, no data have been published regarding the regulation of the human peroxisomal thiolase. After cloning a fragment of the 5′ region involved in the regulation of the human thiolase gene, the effects of different treatments have been studied on the thiolase expression in the hepatoma HepG2 human cell line. Biocomputing analysis indicates that (i) a GRE (glucocorticoid response element) is located at −650 bp upstream of the transcription initiation site; (ii) a C/EBPα (CCAAT/enhancer-binding protein) binding site is located at − 1000 bp upstream of the transcription initiation site – and (iii) there is no putative PPRE (peroxisome proliferator-activated receptor response element). In the human HepG2 cells, thiolase expression is upregulated by glucose and downregulated by insulin and sterols, while dexamethasone and fatty acids have no effect. The ciprofibrate, a peroxisome proliferator, leads only to a weak stimulation of the mRNA expression as compared to thiolase B expression in the rat liver.
Norbert Latruffe. Human Peroxisomal 3-Ketoacyl-CoA Thiolase: Tissue Expression and Metabolic Regulation. Advances in Experimental Medicine and Biology 2020, 1299, 161 -167.
AMA StyleNorbert Latruffe. Human Peroxisomal 3-Ketoacyl-CoA Thiolase: Tissue Expression and Metabolic Regulation. Advances in Experimental Medicine and Biology. 2020; 1299 ():161-167.
Chicago/Turabian StyleNorbert Latruffe. 2020. "Human Peroxisomal 3-Ketoacyl-CoA Thiolase: Tissue Expression and Metabolic Regulation." Advances in Experimental Medicine and Biology 1299, no. : 161-167.
In addition to the traditional treatments of cancer and cancer prevention, the use of natural compounds, especially those found in food, should be considered. To clarify if resveratrol has the potential for cancer prevention and the possibility of use in therapy, the following must be taken into account: data from epidemiology, clinical protocol (case and control), preclinical studies (lab animals), use of established cell lines as models of cancer cells, test tube assays (enzymes activities), and requirements of nanotechnologies in order to discover new drugs to fight cancer. From this perspective and future expected advances, more information is needed such as improved efficacy, methods of application, and the synergistic sensitization of resveratrol as an adjuvant. In addition, resveratrol nanoformulation is considered to overcome its weak bioavailability.
Dominique Vervandier-Fasseur; Norbert Latruffe. The Potential Use of Resveratrol for Cancer Prevention. Molecules 2019, 24, 4506 .
AMA StyleDominique Vervandier-Fasseur, Norbert Latruffe. The Potential Use of Resveratrol for Cancer Prevention. Molecules. 2019; 24 (24):4506.
Chicago/Turabian StyleDominique Vervandier-Fasseur; Norbert Latruffe. 2019. "The Potential Use of Resveratrol for Cancer Prevention." Molecules 24, no. 24: 4506.
Resveratrol has been proposed to prevent tumor growth and the different steps of carcinogenesis; nevertheless, these biological effects are sometimes discordant between different cell types. Several hypotheses and works have suggested that the metabolism of resveratrol could be at the origin of a different cellular response. We show here, using colorectal tumor cell lines, that the biological effects of RSV result mainly from its carriage by carriers of the superfamily of ABC transporter, i.e., P-gP, MRP, or BCRP. Using cell lines overexpressing these different transporters, we have been able to highlight the importance of P-gP in the response of cells to RSV. These results were confirmed by invalidating the gene coding for P-gP, which restored the sensitivity of colorectal cells resistant to the polyphenol. Subsequently, the status of P-glycoprotein expression is an important element to be taken into consideration in the cytotoxic activity of resveratrol in colorectal cancer cells.
Virginie Aires; Didier J Colin; Agnès Doreau; Attilio Di Pietro; Jean-Marie Heydel; Yves Artur; Norbert Latruffe; Dominique Delmas. P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells. Nutrients 2019, 11, 2098 .
AMA StyleVirginie Aires, Didier J Colin, Agnès Doreau, Attilio Di Pietro, Jean-Marie Heydel, Yves Artur, Norbert Latruffe, Dominique Delmas. P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells. Nutrients. 2019; 11 (9):2098.
Chicago/Turabian StyleVirginie Aires; Didier J Colin; Agnès Doreau; Attilio Di Pietro; Jean-Marie Heydel; Yves Artur; Norbert Latruffe; Dominique Delmas. 2019. "P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells." Nutrients 11, no. 9: 2098.
Mediterranean diet (MD) is the most relevant nutritional aspect of the multisecular Mediterranean civilization which includes wine as an element of health and wellbeing when consumed with moderation. Mediterranean meals provide food micronutrients which include polyphenols, especially resveratrol from grape and red wine. MD, also called Cretan diet, has been proven to prevent diseases including cardiovascular pathologies, cancer, and to prevent aging. Interestingly, the grape and more precisely in grape skin contains the highest concentration of RSV. In consequence, red wine it is the most concentrated food source of RSV found in human diet. This review topic deals to how efficient is RSV towards alterations during the aging process; obtained from recent data of clinical trials, preclinical studies and cell culture approach; especially RSV protecting effect on brain aging of elderly; its role on the microglial cells playing a central role in the neuro-inflammation; and in its anti-inflammatory effects on ocular diseases.
Paula Silva; Antoni Sureda; Josep A. Tur; Pierre Andreoletti; Mustapha Cherkaoui-Malki; Norbert Latruffe. How efficient is resveratrol as an antioxidant of the Mediterranean diet, towards alterations during the aging process? Free Radical Research 2019, 53, 1101 -1112.
AMA StylePaula Silva, Antoni Sureda, Josep A. Tur, Pierre Andreoletti, Mustapha Cherkaoui-Malki, Norbert Latruffe. How efficient is resveratrol as an antioxidant of the Mediterranean diet, towards alterations during the aging process? Free Radical Research. 2019; 53 (sup1):1101-1112.
Chicago/Turabian StylePaula Silva; Antoni Sureda; Josep A. Tur; Pierre Andreoletti; Mustapha Cherkaoui-Malki; Norbert Latruffe. 2019. "How efficient is resveratrol as an antioxidant of the Mediterranean diet, towards alterations during the aging process?" Free Radical Research 53, no. sup1: 1101-1112.
There is much literature on the topic of wine and health dating back to the days of Hippocrates, and it is believed that there are unlimited varieties of wine, allowing the association of senses, nutrition, and hedonism
Norbert Latruffe; Jean-Pierre Rifler. Special Issue: Wine and Vine Components and Health. Diseases 2019, 7, 30 .
AMA StyleNorbert Latruffe, Jean-Pierre Rifler. Special Issue: Wine and Vine Components and Health. Diseases. 2019; 7 (1):30.
Chicago/Turabian StyleNorbert Latruffe; Jean-Pierre Rifler. 2019. "Special Issue: Wine and Vine Components and Health." Diseases 7, no. 1: 30.
The mitochondrial-located enzyme Carnitine palmitoyltransferase
Virginie Aires; Dominique Delmas; Fatima Djouadi; Jean Bastin; Mustapha Cherkaoui; Norbert Latruffe. Resveratrol-Dependent Stimulation of Mitochondrial Fatty Acid Oxidation in Deficient Cells. Implication of miRNAs. Proceedings 2019, 11, 5 .
AMA StyleVirginie Aires, Dominique Delmas, Fatima Djouadi, Jean Bastin, Mustapha Cherkaoui, Norbert Latruffe. Resveratrol-Dependent Stimulation of Mitochondrial Fatty Acid Oxidation in Deficient Cells. Implication of miRNAs. Proceedings. 2019; 11 (1):5.
Chicago/Turabian StyleVirginie Aires; Dominique Delmas; Fatima Djouadi; Jean Bastin; Mustapha Cherkaoui; Norbert Latruffe. 2019. "Resveratrol-Dependent Stimulation of Mitochondrial Fatty Acid Oxidation in Deficient Cells. Implication of miRNAs." Proceedings 11, no. 1: 5.
Several of the peroxisomal neurodegenerative disorders are the consequence of a specific deficiency of an enzyme or a transporter involved in peroxisomal beta-oxidation of very long chain fatty acids [1,2].
Mustapha Cherkaoui-Malki; Saad Shaaban; Mounia Tahri-Joutey; Ahmed Elshobaky; Fatima-Ezzahra Saih; Dominique Vervandier-Fasseur; Claus Jacob; Boubker Nasser; Norbert Latruffe; Pierre Andreoletti. Cytoprotective and Antioxidants in Peroxisomal Neurodegenerative Diseases. Proceedings 2019, 11, 33 .
AMA StyleMustapha Cherkaoui-Malki, Saad Shaaban, Mounia Tahri-Joutey, Ahmed Elshobaky, Fatima-Ezzahra Saih, Dominique Vervandier-Fasseur, Claus Jacob, Boubker Nasser, Norbert Latruffe, Pierre Andreoletti. Cytoprotective and Antioxidants in Peroxisomal Neurodegenerative Diseases. Proceedings. 2019; 11 (1):33.
Chicago/Turabian StyleMustapha Cherkaoui-Malki; Saad Shaaban; Mounia Tahri-Joutey; Ahmed Elshobaky; Fatima-Ezzahra Saih; Dominique Vervandier-Fasseur; Claus Jacob; Boubker Nasser; Norbert Latruffe; Pierre Andreoletti. 2019. "Cytoprotective and Antioxidants in Peroxisomal Neurodegenerative Diseases." Proceedings 11, no. 1: 33.
Trans-resveratrol, the most well-known polyphenolic stilbenoid, is found in grapes and accordingly in wine and it is considered to be beneficial for human health, especially towards the aging-linked cell alterations by providing numerous biological activities, such as anti-oxidant, antitumoral, antiviral, anti-inflammatory, neuroprotective, and platelet anti-aggregation properties. Although trans-resveratrol is a promising molecule, it cannot be considered as a drug, due to its weak bio-availability and fast metabolism. To overcome these weaknesses, several research teams have undertaken the synthesis of innovative trans-resveratrol derivatives, with the aim to increase its solubility in water and pharmacological activities towards cell targets. The aim of this review is to show the chronological evolution over the last 25 years of different strategies to develop more efficient trans-resveratrol derivatives towards organism physiology and, therefore, to enhance various pharmacological activities. While the literature on the development of new synthetic derivatives is impressive, this review will focus on selected strategies regarding the substitution of trans-resveratrol phenyl rings, first with hydroxy, methoxy, and halogen groups, and next with functionalized substituents. The effects on cell functions and dysfunctions of interesting resveratrol analogs will be addressed in this review.
Norbert Latruffe; Dominique Vervandier-Fasseur. Strategic Syntheses of Vine and Wine Resveratrol Derivatives to Explore their Effects on Cell Functions and Dysfunctions. Diseases 2018, 6, 110 .
AMA StyleNorbert Latruffe, Dominique Vervandier-Fasseur. Strategic Syntheses of Vine and Wine Resveratrol Derivatives to Explore their Effects on Cell Functions and Dysfunctions. Diseases. 2018; 6 (4):110.
Chicago/Turabian StyleNorbert Latruffe; Dominique Vervandier-Fasseur. 2018. "Strategic Syntheses of Vine and Wine Resveratrol Derivatives to Explore their Effects on Cell Functions and Dysfunctions." Diseases 6, no. 4: 110.
In the prevention of neurodegeneration associated with aging and neurodegenerative diseases (Alzheimer’s disease, Parkinson’s disease), neuronal differentiation is of interest. In this context, neurotrophic factors are a family of peptides capable of promoting the growth, survival, and/or differentiation of both developing and immature neurons. In contrast to these peptidyl compounds, polyphenols are not degraded in the intestinal tract and are able to cross the blood–brain barrier. Consequently, they could potentially be used as therapeutic agents in neurodegenerative pathologies associated with neuronal loss, thus requiring the stimulation of neurogenesis. We therefore studied the ability to induce neuronal differentiation of two major polyphenols present in the Mediterranean diet: resveratrol (RSV), a major compound found in grapes and red wine, and apigenin (API), present in parsley, rosemary, olive oil, and honey. The effects of these compounds (RSV and API: 6.25–50 µM) were studied on murine neuro-2a (N2a) cells after 48 h of treatment without or with 10% fetal bovine serum (FBS). Retinoic acid (RA: 6.25–50 µM) was used as positive control. Neuronal differentiation was morphologically evaluated through the presence of dendrites and axons. Cell growth was determined by cell counting and cell viability by staining with fluorescein diacetate (FDA). Neuronal differentiation was more efficient in the absence of serum than with 10% FBS or 10% delipidized FBS. At concentrations inducing neuronal differentiation, no or slight cytotoxicity was observed with RSV and API, whereas RA was cytotoxic. Without FBS, RSV and API, as well as RA, trigger the neuronal differentiation of N2a cells via signaling pathways simultaneously involving protein kinase A (PKA)/phospholipase C (PLC)/protein kinase C (PKC) and MEK/ERK. With 10% FBS, RSV and RA induce neuronal differentiation via PLC/PKC and PKA/PLC/PKC, respectively. With 10% FBS, PKA and PLC/PKC as well as MEK/ERK signaling pathways were not activated in API-induced neuronal differentiation. In addition, the differentiating effects of RSV and API were not inhibited by cyclo[DLeu5] OP, an antagonist of octadecaneuropeptide (ODN) which is a neurotrophic factor. Moreover, RSV and API do not stimulate the expression of the diazepam-binding inhibitor (DBI), the precursor of ODN. Thus, RSV and API are able to induce neuronal differentiation, ODN and its receptor are not involved in this process, and the activation of the (PLC/PKC) signaling pathway is required, except with apigenin in the presence of 10% FBS. These data show that RSV and API are able to induce neuronal differentiation and therefore mimic neurotrophin activity. Thus, RSV and API could be of interest in regenerative medicine to favor neurogenesis.
Amira Namsi; Thomas Nury; Haithem Hamdouni; Aline Yammine; Anne Vejux; Dominique Vervandier-Fasseur; Norbert Latruffe; Olfa Masmoudi-Kouki; Gérard Lizard. Induction of Neuronal Differentiation of Murine N2a Cells by Two Polyphenols Present in the Mediterranean Diet Mimicking Neurotrophins Activities: Resveratrol and Apigenin. Diseases 2018, 6, 67 .
AMA StyleAmira Namsi, Thomas Nury, Haithem Hamdouni, Aline Yammine, Anne Vejux, Dominique Vervandier-Fasseur, Norbert Latruffe, Olfa Masmoudi-Kouki, Gérard Lizard. Induction of Neuronal Differentiation of Murine N2a Cells by Two Polyphenols Present in the Mediterranean Diet Mimicking Neurotrophins Activities: Resveratrol and Apigenin. Diseases. 2018; 6 (3):67.
Chicago/Turabian StyleAmira Namsi; Thomas Nury; Haithem Hamdouni; Aline Yammine; Anne Vejux; Dominique Vervandier-Fasseur; Norbert Latruffe; Olfa Masmoudi-Kouki; Gérard Lizard. 2018. "Induction of Neuronal Differentiation of Murine N2a Cells by Two Polyphenols Present in the Mediterranean Diet Mimicking Neurotrophins Activities: Resveratrol and Apigenin." Diseases 6, no. 3: 67.
Carnitine palmitoyltransferase-2 (CPT2) is a mitochondrial enzyme involved in long-chain fatty acid entry into mitochondria for their β-oxidation and energy production. Two phenotypes are associated with the extremely reduced CPT2 activity in genetically deficient patients: neonatal lethality or, in milder forms, myopathy. Resveratrol (RSV) is a phytophenol produced by grape plant in response to biotic or abiotic stresses that displays anti-oxidant properties, in particular through AP-1, NFκB, STAT-3, and COX pathways. Some beneficiary effects of RSV are due to its modulation of microRNA (miRNA) expression. RSV can enhance residual CPT2 activities in human fibroblasts derived from CPT2-deficient patients and restores normal fatty acid oxidation rates likely through stimulation of mitochondrial biogenesis. Here, we report changes in miRNA expression linked to CPT2-deficiency, and we identify miRNAs whose expression changed following RSV treatment of control or CPT2-deficient fibroblasts isolated from patients. Our findings suggest that RSV consumption might exert beneficiary effects in patients with CPT2-deficiency.
Virginie Aires; Dominique Delmas; Fatima Djouadi; Jean Bastin; Mustapha Cherkaoui-Malki; Norbert Latruffe. Resveratrol-Induced Changes in MicroRNA Expression in Primary Human Fibroblasts Harboring Carnitine-Palmitoyl Transferase-2 Gene Mutation, Leading to Fatty Acid Oxidation Deficiency. Molecules 2017, 23, 7 .
AMA StyleVirginie Aires, Dominique Delmas, Fatima Djouadi, Jean Bastin, Mustapha Cherkaoui-Malki, Norbert Latruffe. Resveratrol-Induced Changes in MicroRNA Expression in Primary Human Fibroblasts Harboring Carnitine-Palmitoyl Transferase-2 Gene Mutation, Leading to Fatty Acid Oxidation Deficiency. Molecules. 2017; 23 (1):7.
Chicago/Turabian StyleVirginie Aires; Dominique Delmas; Fatima Djouadi; Jean Bastin; Mustapha Cherkaoui-Malki; Norbert Latruffe. 2017. "Resveratrol-Induced Changes in MicroRNA Expression in Primary Human Fibroblasts Harboring Carnitine-Palmitoyl Transferase-2 Gene Mutation, Leading to Fatty Acid Oxidation Deficiency." Molecules 23, no. 1: 7.
Sepsis causes severe dysregulation of organ functions, via the development of oxidative stress and inflammation. These pathophysiological mechanisms are mimicked in mice injected with bacterial lipopolysaccharide (LPS). Here, protective properties of argan oil against LPS-induced oxidative stress and inflammation are explored in the murine model. Mice received standard chow, supplemented with argan oil (AO) or olive oil (OO) for 25 days, before septic shock was provoked with a single intraperitoneal injection of LPS, 16 hours prior to animal sacrifice. In addition to a rise in oxidative stress and inflammatory markers, injected LPS also caused hepatotoxicity, accompanied by hyperglycemia, hypercholesterolemia and hyperuremia. These LPS-associated toxic effects were blunted by AO pretreatment, as corroborated by normal plasma parameters and cell stress markers (glutathione: GSH) and antioxidant enzymology (catalase, CAT; superoxide dismutase, SOD and glutathione peroxidase, GPx). Hematoxylin–eosin staining revealed that AO can protect against acute liver injury, maintaining a normal status, which is pointed out by absent or reduced LPS-induced hepatic damage markers (i.e., alanine aminotransferase (ALT) and aspartate transaminase (AST)). Our work also indicated that AO displayed anti-inflammatory activity, due to down-regulations of genes encoding pro-inflammatory cytokines Interleukin-6 (IL-6) and Tumor Necrosis Factor-α (TNF-α) and in up-regulations of the expression of anti-inflammatory genes encoding Interleukin-4 (IL-4) and Interleukin-10 (IL-10). OO provided animals with similar, though less extensive, protective changes. Collectively our work adds compelling evidence to the protective mechanisms of AO against LPS-induced liver injury and hence therapeutic potentialities, in regard to the management of human sepsis. Activations of IL-4/Peroxisome Proliferator-Activated Receptors (IL-4/PPARs) signaling and, under LPS, an anti-inflammatory IL-10/Liver X Receptor (IL-10/LXR) route, obviously indicated the high potency and plasticity of the anti-inflammatory properties of argan oil.
Soufiane El Kamouni; Riad El Kebbaj; Pierre Andreoletti; Abderrahim El Ktaibi; Issam Rharrassi; Abdelkhalid Essamadi; M’Hammed Saïd El Kebbaj; Stéphane Mandard; Norbert Latruffe; Joseph Vamecq; Boubker Nasser; Mustapha Cherkaoui-Malki; M’Hammed El Kebbaj. Protective Effect of Argan and Olive Oils against LPS-Induced Oxidative Stress and Inflammation in Mice Livers. International Journal of Molecular Sciences 2017, 18, 2181 .
AMA StyleSoufiane El Kamouni, Riad El Kebbaj, Pierre Andreoletti, Abderrahim El Ktaibi, Issam Rharrassi, Abdelkhalid Essamadi, M’Hammed Saïd El Kebbaj, Stéphane Mandard, Norbert Latruffe, Joseph Vamecq, Boubker Nasser, Mustapha Cherkaoui-Malki, M’Hammed El Kebbaj. Protective Effect of Argan and Olive Oils against LPS-Induced Oxidative Stress and Inflammation in Mice Livers. International Journal of Molecular Sciences. 2017; 18 (10):2181.
Chicago/Turabian StyleSoufiane El Kamouni; Riad El Kebbaj; Pierre Andreoletti; Abderrahim El Ktaibi; Issam Rharrassi; Abdelkhalid Essamadi; M’Hammed Saïd El Kebbaj; Stéphane Mandard; Norbert Latruffe; Joseph Vamecq; Boubker Nasser; Mustapha Cherkaoui-Malki; M’Hammed El Kebbaj. 2017. "Protective Effect of Argan and Olive Oils against LPS-Induced Oxidative Stress and Inflammation in Mice Livers." International Journal of Molecular Sciences 18, no. 10: 2181.
Resveratrol is a well-known phenolic stilbene because of its presence in several edible plants and its proposed properties that are beneficial to human health
Dominique Vervandier-Fasseur; Ole Vang; Norbert Latruffe. Special Issue: Improvements for Resveratrol Efficacy. Molecules 2017, 22, 1737 .
AMA StyleDominique Vervandier-Fasseur, Ole Vang, Norbert Latruffe. Special Issue: Improvements for Resveratrol Efficacy. Molecules. 2017; 22 (10):1737.
Chicago/Turabian StyleDominique Vervandier-Fasseur; Ole Vang; Norbert Latruffe. 2017. "Special Issue: Improvements for Resveratrol Efficacy." Molecules 22, no. 10: 1737.
Note: In lieu of an abstract, this is an excerpt from the first page.Excerpt Inflammation (or inflammatory reaction) is the response to body aggression by a pathogen agent, an allergen, a toxic compound, a tissue lesion, etc.
Norbert Latruffe. Natural Products and Inflammation. Molecules 2017, 22, 120 .
AMA StyleNorbert Latruffe. Natural Products and Inflammation. Molecules. 2017; 22 (1):120.
Chicago/Turabian StyleNorbert Latruffe. 2017. "Natural Products and Inflammation." Molecules 22, no. 1: 120.
In this study, we aimed to evaluate the antioxidant and anti-inflammatory properties of Opuntia ficus-indica cactus cladode extracts in microglia BV-2 cells. Inflammation associated with microglia activation in neuronal injury can be achieved by LPS exposure. Using four different structurally and biologically well-characterized LPS serotypes, we revealed a structure-related differential effect of LPS on fatty acid β-oxidation and antioxidant enzymes in peroxisomes: Escherichia coli-LPS decreased ACOX1 activity while Salmonella minnesota-LPS reduced only catalase activity. Different cactus cladode extracts showed an antioxidant effect through microglial catalase activity activation and an anti-inflammatory effect by reducing nitric oxide (NO) LPS-dependent production. These results suggest that cactus extracts may possess a neuroprotective activity through the induction of peroxisomal antioxidant activity and the inhibition of NO production by activated microglial cells.
Fatima-Ezzahra Saih; Pierre Andreoletti; Stéphane Mandard; Norbert Latruffe; M’Hammed Saïd El Kebbaj; Gérard Lizard; Boubker Nasser; Mustapha Cherkaoui-Malki; M’Hammed El Kebbaj. Protective Effect of Cactus Cladode Extracts on Peroxisomal Functions in Microglial BV-2 Cells Activated by Different Lipopolysaccharides. Molecules 2017, 22, 102 .
AMA StyleFatima-Ezzahra Saih, Pierre Andreoletti, Stéphane Mandard, Norbert Latruffe, M’Hammed Saïd El Kebbaj, Gérard Lizard, Boubker Nasser, Mustapha Cherkaoui-Malki, M’Hammed El Kebbaj. Protective Effect of Cactus Cladode Extracts on Peroxisomal Functions in Microglial BV-2 Cells Activated by Different Lipopolysaccharides. Molecules. 2017; 22 (1):102.
Chicago/Turabian StyleFatima-Ezzahra Saih; Pierre Andreoletti; Stéphane Mandard; Norbert Latruffe; M’Hammed Saïd El Kebbaj; Gérard Lizard; Boubker Nasser; Mustapha Cherkaoui-Malki; M’Hammed El Kebbaj. 2017. "Protective Effect of Cactus Cladode Extracts on Peroxisomal Functions in Microglial BV-2 Cells Activated by Different Lipopolysaccharides." Molecules 22, no. 1: 102.