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The loss of agro-biodiversity, climate changes and food insecurity are major challenges in the Mediterranean countries with potentially multidimensional consequences. With respect to salinity, approximately 18 million ha, corresponding to 25 % of total irrigated land in the Mediterranean area, are salt affected. Intensive cropping and the excessive use of expensive inputs such as water and fertilizers aggravate this situation. Understanding how we could improve crop productivity in salinized environments is therefore critical to face these challenges. Our comprehension of fundamental physiological mechanisms in plant salt stress adaptation has greatly advanced over the last decades. However, many of these mechanisms have been linked to salt tolerance in simplified experimental systems whereas they have been rarely functionally proven in real agricultural contexts. The sustainability of farming systems in salt affected Mediterranean soils can be effectively achieved by the use of salt-tolerant halophyte plants even more effective through the use of intercropping, crop rotation and aquaponics.. Moreover, if these halophyte plants are removed from the soil to grow other species, pressure on generating salt-tolerant crop plants would be reduced and much healthier crop plants would be cultivated in less stressed saline soils. This paper will focus on the sustainable practices based on the cultivation of halophytes in saline soils by highlighting some experimental activities carried out at laboratory and field levels in the last few years.
Karim Ben Hamed; Antonella Castagna; Annamaria Ranieri; Pedro García-Caparrós; Marco Santin; José Antonio Hernandez; Gregorio Barba Espin. Halophyte based Mediterranean agriculture in the contexts of food insecurity and global climate change. Environmental and Experimental Botany 2021, 191, 104601 .
AMA StyleKarim Ben Hamed, Antonella Castagna, Annamaria Ranieri, Pedro García-Caparrós, Marco Santin, José Antonio Hernandez, Gregorio Barba Espin. Halophyte based Mediterranean agriculture in the contexts of food insecurity and global climate change. Environmental and Experimental Botany. 2021; 191 ():104601.
Chicago/Turabian StyleKarim Ben Hamed; Antonella Castagna; Annamaria Ranieri; Pedro García-Caparrós; Marco Santin; José Antonio Hernandez; Gregorio Barba Espin. 2021. "Halophyte based Mediterranean agriculture in the contexts of food insecurity and global climate change." Environmental and Experimental Botany 191, no. : 104601.
Plants continuously rely on light as an energy source and as the driver of many processes in their lifetimes. The ability to perceive different light radiations involves several photoreceptors, which in turn activate complex signalling cascades that ultimately lead to a rearrangement in plant metabolism as an adaptation strategy towards specific light conditions. This review, after a brief summary of the structure and mode of action of the different photoreceptors, introduces the main classes of secondary metabolites and specifically focuses on the influence played by the different wavelengths on the content of these compounds in agricultural plants, because of their recognised roles as nutraceuticals.
Marco Santin; Annamaria Ranieri; Antonella Castagna. Anything New under the Sun? An Update on Modulation of Bioactive Compounds by Different Wavelengths in Agricultural Plants. Plants 2021, 10, 1485 .
AMA StyleMarco Santin, Annamaria Ranieri, Antonella Castagna. Anything New under the Sun? An Update on Modulation of Bioactive Compounds by Different Wavelengths in Agricultural Plants. Plants. 2021; 10 (7):1485.
Chicago/Turabian StyleMarco Santin; Annamaria Ranieri; Antonella Castagna. 2021. "Anything New under the Sun? An Update on Modulation of Bioactive Compounds by Different Wavelengths in Agricultural Plants." Plants 10, no. 7: 1485.
The effects of UV radiation on Vitis vinifera cv Tempranillo grapes were studied under field conditions as influenced by ultraviolet (UV) band (UV-A and UV-B), UV-B level (ambient vs enhanced), grape phenological stage (pea-size, veraison, and harvest), grape component (skin, flesh, and seeds), and fraction from which phenolic UV-absorbing compounds (UVACs) were extracted (soluble vs insoluble). Ambient UV-B levels caused stronger effects than ambient UV-A. These effects included increases in flavonol contents (particularly quercetins and kaempferols), the expression of flavonol synthase and chalcone synthase genes (VvFLS4 and VvCHS1), and grape weight and size. In addition, the contents of flavanols and hydroxycinnamic acids increased under UV-B radiation at pea-size stage. All these compounds play physiological roles as antioxidants and UV screens. Synergic effects between UV-B and UV-A were observed. The responses of anthocyanins, stilbenes, and volatile compounds to UV were diffuse or nonexistent. Enhanced UV-B led to rather subtle changes in comparison with ambient UV-B, but differences between both treatments could be demonstrated by multivariate analysis. Pea-size and harvest were the phenological stages where the most significant responses to UV were found, while the skin was the most UV-responsive grape component. Soluble phenolic compounds were much more UV-responsive than insoluble compounds. In conclusion, UV radiation was essential for the induction of specific grape phenolic and volatile compounds. Given the physiological roles of these compounds, as well as their contribution to grape and wine quality, and their potential use as nutraceuticals, our results may have implications on the artificial manipulation of UV radiation.
María‐Ángeles Del‐Castillo‐Alonso; Laura Monforte; Rafael Tomás‐Las‐Heras; Annamaria Ranieri; Antonella Castagna; Javier Martínez‐Abaigar; Encarnación Núñez‐Olivera. Secondary metabolites and related genes in Vitis vinifera L. cv. Tempranillo grapes as influenced by UV radiation and berry development. Physiologia Plantarum 2021, 1 .
AMA StyleMaría‐Ángeles Del‐Castillo‐Alonso, Laura Monforte, Rafael Tomás‐Las‐Heras, Annamaria Ranieri, Antonella Castagna, Javier Martínez‐Abaigar, Encarnación Núñez‐Olivera. Secondary metabolites and related genes in Vitis vinifera L. cv. Tempranillo grapes as influenced by UV radiation and berry development. Physiologia Plantarum. 2021; ():1.
Chicago/Turabian StyleMaría‐Ángeles Del‐Castillo‐Alonso; Laura Monforte; Rafael Tomás‐Las‐Heras; Annamaria Ranieri; Antonella Castagna; Javier Martínez‐Abaigar; Encarnación Núñez‐Olivera. 2021. "Secondary metabolites and related genes in Vitis vinifera L. cv. Tempranillo grapes as influenced by UV radiation and berry development." Physiologia Plantarum , no. : 1.
Ribavirin is an inosine monophosphate dehydrogenase inhibitor with demonstrated activity against coronaviruses, including SARS-CoV-2. Two hospitalized patients with COVID-19 (confirmed by positive tests for SARS-CoV-2) received treatment with ribavirin for inhalation solution (ribavirin aerosol) as part of a compassionate use program. Patient 1 was a 33-year-old male with an unremarkable medical history. Patient 2 was a 29-year-old male who was an active smoker. Both patients were managed according to international and Italian treatment guidelines for COVID-19. In addition, therapy with ribavirin aerosol 100 mg/mL was administered for 30 minutes twice daily for 6 days (ie, 12 doses) in both patients. In order to address concerns about a possible increase in viral dispersal with the use of a nebulizer, healthcare providers remained outside the patient room during ribavirin aerosol administration. Pretreatment chest computed tomography scans showed pseudonodular areas in the upper right lobe with associated ground glass opacities (Patient 1) and multiple areas of parenchymal consolidation in both lower lobes with associated ground glass opacities (Patient 2) that were almost completely resolved on imaging at the end of ribavirin treatment. No adverse reactions to ribavirin treatment were observed in either patient. Both patients recovered fully, and 2 sequential nasopharyngeal swabs obtained after hospital discharge tested negative for SARS-CoV-2. Ribavirin aerosol appears to be efficacious in the treatment of patients with COVID-19. The compassionate use study of ribavirin aerosol is ongoing and will provide additional data across a broader patient population.
Emanuela Messina; Anna Danise; Ferrari; Andrea Andolina; Matteo Chiurlo; Sn Marie Razanakolona; Barakat; Robert J. Israel; Antonella Castagna. Ribavirin Aerosol in the Treatment of SARS-CoV-2. 2021, 1 .
AMA StyleEmanuela Messina, Anna Danise, Ferrari, Andrea Andolina, Matteo Chiurlo, Sn Marie Razanakolona, Barakat, Robert J. Israel, Antonella Castagna. Ribavirin Aerosol in the Treatment of SARS-CoV-2. . 2021; ():1.
Chicago/Turabian StyleEmanuela Messina; Anna Danise; Ferrari; Andrea Andolina; Matteo Chiurlo; Sn Marie Razanakolona; Barakat; Robert J. Israel; Antonella Castagna. 2021. "Ribavirin Aerosol in the Treatment of SARS-CoV-2." , no. : 1.
Chitosan is receiving increasing attention from the food industry for being a biodegradable, non-toxic, antimicrobial biopolymer able to extend the shelf life of, and preserve the quality of, fresh food. However, few studies have investigated the ability of chitosan-based coatings to allow the diffusion of bioactive compounds into the food matrix to improve its nutraceutical quality. This research is aimed at testing whether a hydrophilic molecule (tyrosol) could diffuse from the chitosan-tyrosol coating and cross the tomato peel. To this end, in vitro permeation tests using excised tomato peel and an in vivo application of chitosan-tyrosol coating on tomato fruit, followed by tyrosol quantification in intact fruit, peel and flesh during a seven-day storage at room temperature, were performed. Both approaches demonstrated the ability of tyrosol to permeate across the fruit peel. Along with a decreased tyrosol content in the peel, its concentration within the flesh was increased, indicating an active transfer of tyrosol into this tissue. This finding, together with the maintenance of constant tyrosol levels during the seven-day storage period, is very promising for the use of chitosan formulations to produce functional tomato fruit.
Silvia Tampucci; Antonella Castagna; Daniela Monti; Clementina Manera; Giuseppe Saccomanni; Patrizia Chetoni; Erica Zucchetti; Mariacristina Barbagallo; Laura Fazio; Marco Santin; Annamaria Ranieri. Tyrosol-Enriched Tomatoes by Diffusion across the Fruit Peel from a Chitosan Coating: A Proposal of Functional Food. Foods 2021, 10, 335 .
AMA StyleSilvia Tampucci, Antonella Castagna, Daniela Monti, Clementina Manera, Giuseppe Saccomanni, Patrizia Chetoni, Erica Zucchetti, Mariacristina Barbagallo, Laura Fazio, Marco Santin, Annamaria Ranieri. Tyrosol-Enriched Tomatoes by Diffusion across the Fruit Peel from a Chitosan Coating: A Proposal of Functional Food. Foods. 2021; 10 (2):335.
Chicago/Turabian StyleSilvia Tampucci; Antonella Castagna; Daniela Monti; Clementina Manera; Giuseppe Saccomanni; Patrizia Chetoni; Erica Zucchetti; Mariacristina Barbagallo; Laura Fazio; Marco Santin; Annamaria Ranieri. 2021. "Tyrosol-Enriched Tomatoes by Diffusion across the Fruit Peel from a Chitosan Coating: A Proposal of Functional Food." Foods 10, no. 2: 335.
Background: The ability of elvitegravir/cobicistat/emtricitabine/tenofovir alafenamide (E/C/F/TAF) to maintain virologic suppression in participants with M184V and/or M184I resistance mutations from historical genotypic reports when switching from a tenofovir disoproxil fumarate (TDF)- or abacavir (ABC)-based regimen was investigated. Setting: Phase IIIb, 48-week, open-label, single-arm, multicenter clinical trial (NCT02616029). Methods: Virologically suppressed adults with HIV and documented M184V/I on historical genotypic records switched to E/C/F/TAF from a TDF- or ABC-based regimen. The primary endpoint was HIV-1 RNA <50 copies/mL at week 12 using pure virologic response (PVR). Secondary endpoints included HIV-1 RNA <50 copies/mL at weeks 24/48 (PVR) and at weeks 12/24/48 (FDA snapshot algorithm), and change in CD4+ count at weeks 12/24/48. Results: M184V alone was reported in 82.8% of 64 participants; 9.4% and 7.8% had M184I and M184V/I, respectively, and 43.8% had archived M184V/I (baseline DNA). All (62/62 with available data, 100%, 95% confidence interval 94.2–100%) participants maintained PVR at weeks 12, 24, and 48. By FDA snapshot algorithm, one participant had HIV-1 RNA ≥50 copies/mL (week 12); confirmatory HIV-1 RNA was <50 copies/mL. No significant changes were observed in CD4+ cell count. Drug-related adverse events (AEs) were reported by 10 (15.6%) participants. Six (9.4%) and 5 (7.8%) participants had Grade 3–4 AEs or serious AEs, respectively (none drug related). Conclusions: The presence of the resistance mutations M184V/I did not jeopardize the efficacy of switching to E/C/F/TAF in virologically suppressed adults. High rates of virologic suppression were maintained throughout 48 weeks of therapy and treatment was well tolerated.
Ignacio Perez-Valero; Josep M. Llibre; Antonella Castagna; Federico Pulido; Jean-Michel Molina; Stefan Esser; Nicolas Margot; Yongwu Shao; Lauren Temme; David Piontkowsky; Ian R. McNicholl; Richard Haubrich. Switching to Elvitegravir/Cobicistat/Emtricitabine/Tenofovir Alafenamide in Adults With HIV and M184V/I Mutation. JAIDS Journal of Acquired Immune Deficiency Syndromes 2020, 86, 490 -495.
AMA StyleIgnacio Perez-Valero, Josep M. Llibre, Antonella Castagna, Federico Pulido, Jean-Michel Molina, Stefan Esser, Nicolas Margot, Yongwu Shao, Lauren Temme, David Piontkowsky, Ian R. McNicholl, Richard Haubrich. Switching to Elvitegravir/Cobicistat/Emtricitabine/Tenofovir Alafenamide in Adults With HIV and M184V/I Mutation. JAIDS Journal of Acquired Immune Deficiency Syndromes. 2020; 86 (4):490-495.
Chicago/Turabian StyleIgnacio Perez-Valero; Josep M. Llibre; Antonella Castagna; Federico Pulido; Jean-Michel Molina; Stefan Esser; Nicolas Margot; Yongwu Shao; Lauren Temme; David Piontkowsky; Ian R. McNicholl; Richard Haubrich. 2020. "Switching to Elvitegravir/Cobicistat/Emtricitabine/Tenofovir Alafenamide in Adults With HIV and M184V/I Mutation." JAIDS Journal of Acquired Immune Deficiency Syndromes 86, no. 4: 490-495.
In the last decades, UV-B radiation has attracted attention due to its potential to increase nutraceutical values of fruit and vegetables, especially by inducing the accumulation of phenolics in a structure-dependent way. However, most current studies have investigated the UV-B-driven changes only in the peel or focusing on individual phenolic classes. Adopting an “-omics” approach, this work aimed to deepen the knowledge about the effects of UV-B radiation on the phenolic profile in the pulp of peach fruit. Based on these considerations, melting flesh yellow peaches (Prunus persica L., cv. Fairtime) were subjected to either a 10- or 60-min UV-B treatment (1.39 and 8.33 kJ m–2, respectively), and sampled at different time points from the exposure. A UHPLC-ESI/QTOF-MS analysis coupled with a phenolics-specific database for the annotation of compounds and a multivariate discriminant analysis revealed a marked effect of UV-B radiation on the phenolic profiles of peach pulp. Particularly, a general, transient increase was observed after 24 h from the irradiation, especially for flavanols, flavonols, and flavones. Such behavior diverges from what was observed in the peel, where an overall increase of phenolics was observed after 36 h from the irradiation. Concerning the flavonols in the pulp, UV-B exposure stimulated a specific accumulation of isorhamnetin and kaempferol derivatives, with variations imposed by the different sugar moiety bound. Anthocyanins, which were the second most abundant flavonoid group after flavonols, displayed a general decrease after 36 h that was not attributable to specific molecules. The UV-B treatments also increased the glycoside/aglycone ratio of flavonols and anthocyanins after 24 h, by increasing the glycoside concentration of both, flavonols and anthocyanins, and decreasing the aglycone concentration of anthocyanins. In support of the biochemical results, targeted gene expression analysis by RT-qPCR revealed an UV-B-induced activation of many genes involved in the flavonoid pathway, e.g., CHS, F3H, F3′H, DFR, as well as some MYB transcription factors and few genes involved in the UV-B perception. Generally, all the flavonoid-related and MYB genes showed a transient UV-B dose-dependent activation after 6 h from the irradiation, similarly to what was observed in the peel.
Marco Santin; Antonella Castagna; Begoña Miras-Moreno; Gabriele Rocchetti; Luigi Lucini; Marie-Theres Hauser; Annamaria Ranieri. Beyond the Visible and Below the Peel: How UV-B Radiation Influences the Phenolic Profile in the Pulp of Peach Fruit. A Biochemical and Molecular Study. Frontiers in Plant Science 2020, 11, 579063 .
AMA StyleMarco Santin, Antonella Castagna, Begoña Miras-Moreno, Gabriele Rocchetti, Luigi Lucini, Marie-Theres Hauser, Annamaria Ranieri. Beyond the Visible and Below the Peel: How UV-B Radiation Influences the Phenolic Profile in the Pulp of Peach Fruit. A Biochemical and Molecular Study. Frontiers in Plant Science. 2020; 11 ():579063.
Chicago/Turabian StyleMarco Santin; Antonella Castagna; Begoña Miras-Moreno; Gabriele Rocchetti; Luigi Lucini; Marie-Theres Hauser; Annamaria Ranieri. 2020. "Beyond the Visible and Below the Peel: How UV-B Radiation Influences the Phenolic Profile in the Pulp of Peach Fruit. A Biochemical and Molecular Study." Frontiers in Plant Science 11, no. : 579063.
In this study, the effect of different drying processes (freeze-drying (FD), microwave-assisted drying (MWD) and classic hot air drying (HAD)) on the polyphenols, flavonoids, and amino acids content was investigated on bee-collected chestnut, willow and ivy pollen for human consumption. Furthermore, the pollen chemical properties were monitored after three and six months of storage, and then analyzed using a multivariate approach. Chestnut pollen was the richest source of polyphenols, flavonoids, and rutin, while ivy pollen contained the highest amount of total and free amino acids, and total and free proline. Drying and storage affected pollen chemical composition with species-dependent effects. MWD allowed the best retention of flavonoids in chestnut pollen for up to six months of storage. All drying techniques led to a depletion of flavonoids in willow pollen; however, MWD ensured the highest flavonoids content after six months. FD and MWD did not lead to flavonoids depletion in ivy pollen during storage. Additionally, storage did not affect the rutin content, which was highest in FD willow samples after six months. Notably, both FD and MWD techniques are efficient in preserving amino acids-related quality of bee pollen up to six months of storage.
Antonella Castagna; Giovanni Benelli; Giuseppe Conte; Cristina Sgherri; Francesca Signorini; Cristiano Nicolella; Annamaria Ranieri; Angelo Canale. Drying Techniques and Storage: Do They Affect the Nutritional Value of Bee-Collected Pollen? Molecules 2020, 25, 4925 .
AMA StyleAntonella Castagna, Giovanni Benelli, Giuseppe Conte, Cristina Sgherri, Francesca Signorini, Cristiano Nicolella, Annamaria Ranieri, Angelo Canale. Drying Techniques and Storage: Do They Affect the Nutritional Value of Bee-Collected Pollen? Molecules. 2020; 25 (21):4925.
Chicago/Turabian StyleAntonella Castagna; Giovanni Benelli; Giuseppe Conte; Cristina Sgherri; Francesca Signorini; Cristiano Nicolella; Annamaria Ranieri; Angelo Canale. 2020. "Drying Techniques and Storage: Do They Affect the Nutritional Value of Bee-Collected Pollen?" Molecules 25, no. 21: 4925.
Stefania Frassinetti; Antonella Castagna; Marco Santin; Luisa Pozzo; Irene Baratto; Vincenzo Longo; Annamaria Ranieri. Gelatin-based coating enriched with blueberry juice preserves the nutraceutical quality and reduces the microbial contamination of tomato fruit. Natural Product Research 2020, 1 -5.
AMA StyleStefania Frassinetti, Antonella Castagna, Marco Santin, Luisa Pozzo, Irene Baratto, Vincenzo Longo, Annamaria Ranieri. Gelatin-based coating enriched with blueberry juice preserves the nutraceutical quality and reduces the microbial contamination of tomato fruit. Natural Product Research. 2020; ():1-5.
Chicago/Turabian StyleStefania Frassinetti; Antonella Castagna; Marco Santin; Luisa Pozzo; Irene Baratto; Vincenzo Longo; Annamaria Ranieri. 2020. "Gelatin-based coating enriched with blueberry juice preserves the nutraceutical quality and reduces the microbial contamination of tomato fruit." Natural Product Research , no. : 1-5.
UV-B-driven modulation of secondary metabolism in peach fruit by enhancing the biosynthesis of specific phenolic subclasses, is attracting interest among consumers. However, current literature explored the UV-B-induced metabolic changes only in peach skin subjected to direct UV-B irradiation. Accordingly, this study aimed to understand whether UV-B radiation penetrates the fruit skin and is able to induce metabolic changes also within the inner flesh. Peaches were UV-B-irradiated either 10 or 60 min, and the flesh was sampled after 24 and 36 h. Non-targeted metabolomics revealed that UV-B has a strong impact on peach flesh metabolome, determining an initial decrease after 24 h, followed by an overall increase after 36 h, particularly for terpenoids, phenylpropanoids, phytoalexins and fatty acids in the 60 min UV-B-treated samples (+150.02, +99.14, +43.79 and +25.44 log2FC, respectively). Transmittance analysis indicated that UV-B radiation does not penetrate below the skin, suggesting a possible signalling pathway between tissues.
Marco Santin; Annamaria Ranieri; Marie-Theres Hauser; Begoña Miras-Moreno; Gabriele Rocchetti; Luigi Lucini; Åke Strid; Antonella Castagna. The outer influences the inner: Postharvest UV-B irradiation modulates peach flesh metabolome although shielded by the skin. Food Chemistry 2020, 338, 127782 .
AMA StyleMarco Santin, Annamaria Ranieri, Marie-Theres Hauser, Begoña Miras-Moreno, Gabriele Rocchetti, Luigi Lucini, Åke Strid, Antonella Castagna. The outer influences the inner: Postharvest UV-B irradiation modulates peach flesh metabolome although shielded by the skin. Food Chemistry. 2020; 338 ():127782.
Chicago/Turabian StyleMarco Santin; Annamaria Ranieri; Marie-Theres Hauser; Begoña Miras-Moreno; Gabriele Rocchetti; Luigi Lucini; Åke Strid; Antonella Castagna. 2020. "The outer influences the inner: Postharvest UV-B irradiation modulates peach flesh metabolome although shielded by the skin." Food Chemistry 338, no. : 127782.
During the last decades, many studies investigated the effects of UV-B on the above-ground organs of plants, directly reached by the radiation but, to the best of our knowledges, the influence of mild UV-B doses on root hormones was not explored. Consequently, this research aimed at understanding whether low, not-stressful doses of UV-B radiation applied above-ground influenced the hormone concentrations in leaves and roots of Micro-Tom tomato (Solanum lycopersicum L.) plants during 11 days of treatment and after 3 days of recovery. In particular, ethylene, abscisic acid, jasmonic acid, salicylic acid and indoleacetic acid were investigated. The unchanged levels of chlorophyll a and b, lutein, total xanthophylls and carotenoids, as well as the similar H2O2 concentration between control and treated groups suggest that the UV-B dose applied was well tolerated by the plants. Leaf ethylene emission decreased after 8 and 11 days of irradiation, while no effect was found in roots. Conversely, indoleacetic acid underwent a significant reduction in both organs, though in the roots the decrease occurred only at the end of the recovery period. Salicylic acid increased transiently in both leaves and roots on day 8. Changes in leaf and root hormone levels induced by UV-B radiation were not accompanied by marked alterations of plant architecture. The results show that irradiation of above-ground organs with low UV-B doses can affect the hormone concentrations also in roots, with likely implications in stress and acclimation responses mediated by these signal molecules.
Alessia Mannucci; Lorenzo Mariotti; Antonella Castagna; Marco Santin; Alice Trivellini; Thais Huarancca Reyes; Anna Mensuali-Sodi; Annamaria Ranieri; Mike Frank Quartacci. Hormone profile changes occur in roots and leaves of Micro-Tom tomato plants when exposing the aerial part to low doses of UV-B radiation. Plant Physiology and Biochemistry 2020, 148, 291 -301.
AMA StyleAlessia Mannucci, Lorenzo Mariotti, Antonella Castagna, Marco Santin, Alice Trivellini, Thais Huarancca Reyes, Anna Mensuali-Sodi, Annamaria Ranieri, Mike Frank Quartacci. Hormone profile changes occur in roots and leaves of Micro-Tom tomato plants when exposing the aerial part to low doses of UV-B radiation. Plant Physiology and Biochemistry. 2020; 148 ():291-301.
Chicago/Turabian StyleAlessia Mannucci; Lorenzo Mariotti; Antonella Castagna; Marco Santin; Alice Trivellini; Thais Huarancca Reyes; Anna Mensuali-Sodi; Annamaria Ranieri; Mike Frank Quartacci. 2020. "Hormone profile changes occur in roots and leaves of Micro-Tom tomato plants when exposing the aerial part to low doses of UV-B radiation." Plant Physiology and Biochemistry 148, no. : 291-301.
To evaluate efficacy and drug-resistance mutations selected at virological failure (VF) in HIV-infected patients switched to dolutegravir plus rilpivirine (DTG+RPV) or lamivudine (DTG+3TC) while virologically suppressed. Retrospective study of HIV-infected patients prospectively followed at the Infectious Disease Department of the San Raffaele Scientific Institute who were switched to DTG+3TC or DTG+RPV with HIV-RNA 50 copies/mL in two consecutive determinations or in a single determination if followed by treatment modification or >1000 copies/mL in a single determination. Three hundred seventy-four patients were analyzed, 307 switched to DTG+3TC, 67 to DTG+RPV; 220 had historical resistance documented. The median time with HIV-RNA < 50 copies/ml before the switch was 4.52 years (IQR 1.93 - 8.14). VF occurred in 17 patients, after 1.74 years (IQR 0.90 - 2.46) of follow-up, in the 3TC group (incidence rate [95%CI]: 3.34 [2.08-5.37] per 100-PYFU) and in two patients, after 1.78 years (IQR 1.10 - 2.99) of follow-up, in the RPV group (incidence rate [95%CI]: 1.57 [0.4-6.28] per 100-PYFU). The 48-week estimated probabilities to maintain virological suppression during treatment with 2DR were 97.8% [95%CI: 95.1%-99.0%] and 98.3% [95%CI: 88.6%-99.8%] in the 3TC vs RPV group; p=0.311. Patients who experienced VF had, at switch, undetectable HIV-RNA since 0.71 (0.23-1.07) years vs 1.49 (0.64-2.2) years in those who did not experience VF (p=0.001). In the 3TC group, VF was not associated with the presence of historical resistance to nucleoside analogues and DTG-resistant variants were not selected at VF. One VF to DTG+RPV occurred because of historical resistance to RPV and was accompanied by newly selected 140A and 148R mutations. VF was infrequent with these regimens and was negatively associated with duration of viral undetectability. Drug-resistance mutations selected at failure of these regimens were those expectable in case of failure of any regimen including DTG, 3TC or RPV, but the impact of resistance to nucleoside reverse transcriptase inhibitors on efficacy of DTG+3TC seems lower than expected.
Nadia Galizzi; Andrea Poli; Laura Galli; Camilla Muccini; Andrea Mastrangelo; Raffaele Dell'Acqua; Myriam Maillard; Simona Bossolasco; Paola Cinque; Adriano Lazzarin; Antonella Castagna; Nicola Gianotti. Retrospective study on the outcome of two-drug regimens based on dolutegravir plus one reverse transcriptase inhibitor in virologically-suppressed HIV-infected patients. International Journal of Antimicrobial Agents 2020, 55, 105893 .
AMA StyleNadia Galizzi, Andrea Poli, Laura Galli, Camilla Muccini, Andrea Mastrangelo, Raffaele Dell'Acqua, Myriam Maillard, Simona Bossolasco, Paola Cinque, Adriano Lazzarin, Antonella Castagna, Nicola Gianotti. Retrospective study on the outcome of two-drug regimens based on dolutegravir plus one reverse transcriptase inhibitor in virologically-suppressed HIV-infected patients. International Journal of Antimicrobial Agents. 2020; 55 (3):105893.
Chicago/Turabian StyleNadia Galizzi; Andrea Poli; Laura Galli; Camilla Muccini; Andrea Mastrangelo; Raffaele Dell'Acqua; Myriam Maillard; Simona Bossolasco; Paola Cinque; Adriano Lazzarin; Antonella Castagna; Nicola Gianotti. 2020. "Retrospective study on the outcome of two-drug regimens based on dolutegravir plus one reverse transcriptase inhibitor in virologically-suppressed HIV-infected patients." International Journal of Antimicrobial Agents 55, no. 3: 105893.
Large amounts of peach (Prunus persica) leaves and stems are by-products deriving from peach tree cultivation and canned industries. This work aimed to evaluate mineral nutrients, phenolic and volatile profile and antioxidant activities from the by-products of five peach cultivars (Early Maycrest, Sweet Cap, O’Henry, Flordastar and Rubirich). Minerals showed significant variations with respect to peach by-product. N showed higher contents in peach leaves among macronutrients, while Mn showed higher contents among micronutrients. Stems had high levels of Ca and traces of micronutrient levels. The HPLC-DAD phenols analysis showed twelve compounds identified (neochlorogenic and chlorogenic acids, catechin and epicatechin, gallic, caffeic, syringic, ferulic and coumaric acids, quercetin-3-rutinoside, quercetin-3-galactoside, cyanidin-3-glucoside, cyanidin-3-galactoside). Flavonols showed the highest values (quercetin-3-galactoside with 70.79–232.16 mg 100g-1 DW, quercetin-3-rutinoside with 63.88–206.37 mg 100g-1 DW), while the least content was observed for anthocyanins. Cultivar had a significantly (P < 0.05) impact on phenolic compounds. Comparing by-products, stems showed higher levels of phenols. The GC-MS volatile compounds analysis revealed 43 compounds in different percentages and occurrences, depending on the cultivar and the by-product. Benzaldehyde was detected as the major volatile leaf component (70–95%), whereas myrcene (18–21%) and terpinolene (18–26%) were found to be the most important compounds in stems. Methanolic extracts of mature leaves were characterized by lower antioxidant capacity. Finally, peach by-products could represent a natural source of minerals, volatiles and phenolic compounds with high antioxidant activities having a great potential use in food products as natural flavouring agents and as nutraceutical supplements and pharmaceutical and cosmetic molecules.
Samira Maatallah; Samia Dabbou; Antonella Castagna; Monia Guizani; Hichem Hajlaoui; Anna Maria Ranieri; Guido Flamini. Prunus persica by-products: A source of minerals, phenols and volatile compounds. Scientia Horticulturae 2019, 261, 109016 .
AMA StyleSamira Maatallah, Samia Dabbou, Antonella Castagna, Monia Guizani, Hichem Hajlaoui, Anna Maria Ranieri, Guido Flamini. Prunus persica by-products: A source of minerals, phenols and volatile compounds. Scientia Horticulturae. 2019; 261 ():109016.
Chicago/Turabian StyleSamira Maatallah; Samia Dabbou; Antonella Castagna; Monia Guizani; Hichem Hajlaoui; Anna Maria Ranieri; Guido Flamini. 2019. "Prunus persica by-products: A source of minerals, phenols and volatile compounds." Scientia Horticulturae 261, no. : 109016.
Linseed (Linum usitatissimum L.) is becoming more and more important in the health food market as a functional food, since its seeds and oil represent a rich source of bioactive compounds. Its chemical composition is strongly correlated with, and dependent on, genetic characteristics. The aim of this study was to evaluate the variation in seed yield, oil content, fatty acid composition and secondary metabolite profiles between a low-linolenic linseed variety, belonging to the Solin-type group (Solal), and a high-linolenic traditional one (Bethune), cultivated, both as spring crops, in open field conditions of Central Italy. The achieved results pointed out the different behavior of the two varieties in terms of growth cycle, oil content, and some important yield components, such as capsule number per plant and thousand seed weight. There were also significant differences in seed composition regarding total phenols, total flavonoids, antioxidant activities as well as in carotenoid, tocopherol, and tocotrienol profiles between the two varieties. In particular, Solal was characterized by the greatest contents of oil, phenols, flavonoids, α- and δ- tocotrienol, together with the highest antioxidant activity. Bethune, on the contrary, showed the highest amounts of carotenoids (lutein and β-carotene). These results indicate a clear effect of the genetic characteristics on the biosynthesis of these secondary metabolites and, consequently, on the related antioxidant activity. Our findings suggest that the mutation process, responsible for the selection of the low-linolenic cultivar, is able to modify the biosynthetic pathways of carotenoids and phenolics.
Silvia Tavarini; Antonella Castagna; Giuseppe Conte; Lara Foschi; Chiara Sanmartin; Luca Incrocci; Annamaria Ranieri; Andrea Serra; Luciana G. Angelini. Evaluation of Chemical Composition of Two Linseed Varieties as Sources of Health-Beneficial Substances. Molecules 2019, 24, 3729 .
AMA StyleSilvia Tavarini, Antonella Castagna, Giuseppe Conte, Lara Foschi, Chiara Sanmartin, Luca Incrocci, Annamaria Ranieri, Andrea Serra, Luciana G. Angelini. Evaluation of Chemical Composition of Two Linseed Varieties as Sources of Health-Beneficial Substances. Molecules. 2019; 24 (20):3729.
Chicago/Turabian StyleSilvia Tavarini; Antonella Castagna; Giuseppe Conte; Lara Foschi; Chiara Sanmartin; Luca Incrocci; Annamaria Ranieri; Andrea Serra; Luciana G. Angelini. 2019. "Evaluation of Chemical Composition of Two Linseed Varieties as Sources of Health-Beneficial Substances." Molecules 24, no. 20: 3729.
The plants (green and red lettuce) were exposed to daily doses of UV‐B radiation for 1 hr for two weeks during growth. UV‐B‐treated green lettuces at the second week presented a greater content of quercetin than the control plants. Caffeoyltartaric acid, caffeoylquinic acid and caffeoylmalic acid also showed a significant accumulation in green lettuce after two weeks of UV‐B treatment in comparison to those non‐treated. Regarding carotenoid content, lutein, neoxanthin, and V + A + Z showed significant increased values after two weeks of UV‐B treatment in comparison to the non‐treated green lettuces. However, chlorophylls and carotenoids content did not present significant difference between the analyzed weeks for the red lettuces. In other words, quercetins, as well as caffeoyltartaric acid, caffeoylquinic acid and cyanidin glycoside of red lettuce showed a significant increase after UV‐B treatment at both weeks in comparison with control red lettuces. Practical applications The impact of UV‐B on plants depends on the fluency rate, exposure time, wavelength of UV‐B radiation, and the amount of UV‐B relative to photosynthetic active radiation. Since carotenoids, chlorophylls, and flavonoid compounds can be increased by ecologically relevant levels of UV‐B in glasshouse production, this study was designed to verify whether supplemental UV‐B radiation was effective in increasing the concentration of health‐promoting compounds in green and red lettuce.
Carolina Fagundes Assumpção; Renato Queiroz Assis; Vanessa Stahl Hermes Poletto; Antonella Castagna; Annamaria Ranieri; Susanne Neugart; Simone Hickmann Flôres; Alessandro Oliveira Rios. Application of supplemental UV‐B radiation in pre‐harvest to enhance health‐promoting compounds accumulation in green and red lettuce. Journal of Food Processing and Preservation 2019, 43, 1 .
AMA StyleCarolina Fagundes Assumpção, Renato Queiroz Assis, Vanessa Stahl Hermes Poletto, Antonella Castagna, Annamaria Ranieri, Susanne Neugart, Simone Hickmann Flôres, Alessandro Oliveira Rios. Application of supplemental UV‐B radiation in pre‐harvest to enhance health‐promoting compounds accumulation in green and red lettuce. Journal of Food Processing and Preservation. 2019; 43 (11):1.
Chicago/Turabian StyleCarolina Fagundes Assumpção; Renato Queiroz Assis; Vanessa Stahl Hermes Poletto; Antonella Castagna; Annamaria Ranieri; Susanne Neugart; Simone Hickmann Flôres; Alessandro Oliveira Rios. 2019. "Application of supplemental UV‐B radiation in pre‐harvest to enhance health‐promoting compounds accumulation in green and red lettuce." Journal of Food Processing and Preservation 43, no. 11: 1.
UV-B radiation reduces the softening of peach fruit, thus improving their quality and shelf-life.
Marco Santin; Tommaso Giordani; Andrea Cavallini; Rodolfo Bernardi; Antonella Castagna; Marie-Theres Hauser; Annamaria Ranieri. UV-B exposure reduces the activity of several cell wall-dismantling enzymes and affects the expression of their biosynthetic genes in peach fruit (Prunus persica L., cv. Fairtime, melting phenotype). Photochemical & Photobiological Sciences 2019, 18, 1280 -1289.
AMA StyleMarco Santin, Tommaso Giordani, Andrea Cavallini, Rodolfo Bernardi, Antonella Castagna, Marie-Theres Hauser, Annamaria Ranieri. UV-B exposure reduces the activity of several cell wall-dismantling enzymes and affects the expression of their biosynthetic genes in peach fruit (Prunus persica L., cv. Fairtime, melting phenotype). Photochemical & Photobiological Sciences. 2019; 18 (5):1280-1289.
Chicago/Turabian StyleMarco Santin; Tommaso Giordani; Andrea Cavallini; Rodolfo Bernardi; Antonella Castagna; Marie-Theres Hauser; Annamaria Ranieri. 2019. "UV-B exposure reduces the activity of several cell wall-dismantling enzymes and affects the expression of their biosynthetic genes in peach fruit (Prunus persica L., cv. Fairtime, melting phenotype)." Photochemical & Photobiological Sciences 18, no. 5: 1280-1289.
Flaxseed is well known for its nutritional/nutraceutical value and it is commonly cultivated for oil production. This process generates considerable amounts of by-products, such as oil cake. This study aimed at quantifying the nutrients and nutraceuticals content of cake and at checking their maintenance during storage in paper or polypropylene bags, at room temperature. Freshly-prepared cake was enriched in phenols (+250%), leading to increased antioxidant activity (+148%), while carotenoids and tocopherols underwent a remarkable reduction, as compared to seeds. During storage, the content of phenols, carotenoids, chlorophylls and tocopherols decreased, though α-tocopherol markedly increased (+86% and +99% after 3 months in paper and plastic, respectively), due to a possible conversion of γ-tocopherol to the α-form. Cake maintained an excellent fatty acid composition, also during storage, with optimal omega-6/omega-3 ratios. After 3 months, a peak of conjugated dienes was observed, while peroxides and MDA decreased with storage. Thanks to the presence of antioxidant molecules, polyunsaturated fatty acids and proteins, this by-product could represent an interesting feed supplement, also in the form of stored product. Interestingly, no differences between paper- or plastic-stored cakes occurred, suggesting the preferential use of the eco-friendly material.
Alessia Mannucci; Antonella Castagna; Marco Santin; Andrea Serra; Marcello Mele; Annamaria Ranieri. Quality of flaxseed oil cake under different storage conditions. LWT 2019, 104, 84 -90.
AMA StyleAlessia Mannucci, Antonella Castagna, Marco Santin, Andrea Serra, Marcello Mele, Annamaria Ranieri. Quality of flaxseed oil cake under different storage conditions. LWT. 2019; 104 ():84-90.
Chicago/Turabian StyleAlessia Mannucci; Antonella Castagna; Marco Santin; Andrea Serra; Marcello Mele; Annamaria Ranieri. 2019. "Quality of flaxseed oil cake under different storage conditions." LWT 104, no. : 84-90.
To investigate the association between total, direct and indirect bilirubin and the presence of carotid lesions in a large sample of HIV-1 infected patients on virological suppression. Retrospective study on adult HIV-1 infected patients, with a carotid ultrasound (CUS) examination performed between January 2008 to August 2016, with HIV-RNA Methods: Intima media thickness (IMT) was measured in 4 segments: carotid common artery and bifurcation on left and right sides. Carotid lesion was defined as an IMT ≥1.5 mm in ≥1 region at CUS. Patients were classified as: normal, if all bilirubin values before CUS were below the upper normal limit (UNL); with hyperbilirubinemia if ≥1 bilirubin value above UNL before CUS was recorded. Multivariate logistic regression was used to determine whether hyperbilirubinemia showed association with the presence of ≥1 carotid lesion, after adjusting for confounding factors. Overall, 903 patients were evaluated, 511 with ≥1 and 392 without carotid lesions. At multivariate analysis, total [adjusted odds ratio (AOR) (95% confidence interval, CI) 0.57 (0.36-0.90), p=0.016] and indirect hyperbilirubinemia before CUS [AOR (95%CI) 0.62 (0.40-0.97), p=0.036] were associated with a lower risk of carotid lesions in addition to younger age, negative HCV antibodies, higher nadir CD4+, lower low-density lipoprotein cholesterol, higher high-density lipoprotein cholesterol, lower triglycerides and no use of statin; no effect of atazanavir treatment on carotid lesions was detected. In HIV-1 treated patients, total or indirect hyperbilirubinemia was likely associated with the absence of carotid lesions.
Camilla Muccini; Laura Galli; Andrea Poli; Alessia Carbone; Myriam Maillard; Maria Cristina Giusti; Vincenzo Spagnuolo; Alba Bigoloni; Monica Guffanti; Nicola Gianotti; Adriano Lazzarin; Antonella Castagna. Brief Report: Hyperbilirubinemia Is Associated With a Decreased Risk of Carotid Atherosclerosis in HIV-Infected Patients on Virological Suppression. JAIDS Journal of Acquired Immune Deficiency Syndromes 2018, 79, 617 -623.
AMA StyleCamilla Muccini, Laura Galli, Andrea Poli, Alessia Carbone, Myriam Maillard, Maria Cristina Giusti, Vincenzo Spagnuolo, Alba Bigoloni, Monica Guffanti, Nicola Gianotti, Adriano Lazzarin, Antonella Castagna. Brief Report: Hyperbilirubinemia Is Associated With a Decreased Risk of Carotid Atherosclerosis in HIV-Infected Patients on Virological Suppression. JAIDS Journal of Acquired Immune Deficiency Syndromes. 2018; 79 (5):617-623.
Chicago/Turabian StyleCamilla Muccini; Laura Galli; Andrea Poli; Alessia Carbone; Myriam Maillard; Maria Cristina Giusti; Vincenzo Spagnuolo; Alba Bigoloni; Monica Guffanti; Nicola Gianotti; Adriano Lazzarin; Antonella Castagna. 2018. "Brief Report: Hyperbilirubinemia Is Associated With a Decreased Risk of Carotid Atherosclerosis in HIV-Infected Patients on Virological Suppression." JAIDS Journal of Acquired Immune Deficiency Syndromes 79, no. 5: 617-623.
Corrigendum: UV-B Pre-treatment Alters Phenolics Response to Monilinia fructicola Infection in a Structure-Dependent Way in Peach Skin
Marco Santin; Susanne Neugart; Antonella Castagna; Martina Barilari; Sabrina Sarrocco; Giovanni Vannacci; Monika Schreiner; Annamaria Ranieri. Corrigendum: UV-B Pre-treatment Alters Phenolics Response to Monilinia fructicola Infection in a Structure-Dependent Way in Peach Skin. Frontiers in Plant Science 2018, 9, 1 .
AMA StyleMarco Santin, Susanne Neugart, Antonella Castagna, Martina Barilari, Sabrina Sarrocco, Giovanni Vannacci, Monika Schreiner, Annamaria Ranieri. Corrigendum: UV-B Pre-treatment Alters Phenolics Response to Monilinia fructicola Infection in a Structure-Dependent Way in Peach Skin. Frontiers in Plant Science. 2018; 9 ():1.
Chicago/Turabian StyleMarco Santin; Susanne Neugart; Antonella Castagna; Martina Barilari; Sabrina Sarrocco; Giovanni Vannacci; Monika Schreiner; Annamaria Ranieri. 2018. "Corrigendum: UV-B Pre-treatment Alters Phenolics Response to Monilinia fructicola Infection in a Structure-Dependent Way in Peach Skin." Frontiers in Plant Science 9, no. : 1.
Ultraviolet-B (UV-B) radiation impacts the plant behaviour in many ways, including modifying their secondary metabolism. Although several studies have quantified the UV-B effects on phenolic composition, most of them focused on leaves or investigated a limited amount of phenolics. The present work aimed to investigate the phenolic changes after two postharvest UV-B treatments, 10 and 60 min (1.39 kJ m−2 and 8.33 kJ m−2, respectively), on peach (Prunus persica cv Fairtime) fruit with a non-targeted, whole profiling approach, and targeted gene expression analysis on skin. After both UV-B exposures, peach fruit were harvested at 24 and 36 h for “phenol-omics” analysis, while additional 6 h and 12 h recovery times were used for gene expression analysis. Our results revealed that both UV-B exposures resulted in a decrease of several phenolic compounds, such as anthocyanins, after 24 h from the exposure. In contrast, the expression of the UV-B signalling components, the phenylpropanoid biosynthesis genes and their transcriptional regulators increased 6 h after the treatment, mostly with a UV-B-dose dependent behaviour, preceding an accumulation of most phenolics in both the UV-B treatments at 36 h compared to 24 h. Orthogonal projections to latent structures discriminant analysis (OPLS-DA) revealed that flavonoids, particularly anthocyanins, were the main phenolic subclasses accumulated after UV-B exposure.
Marco Santin; Luigi Lucini; Antonella Castagna; Gabriele Rocchetti; Marie-Theres Hauser; Annamaria Ranieri. Comparative “phenol-omics” and gene expression analyses in peach (Prunus persica) skin in response to different postharvest UV-B treatments. Plant Physiology and Biochemistry 2018, 135, 511 -519.
AMA StyleMarco Santin, Luigi Lucini, Antonella Castagna, Gabriele Rocchetti, Marie-Theres Hauser, Annamaria Ranieri. Comparative “phenol-omics” and gene expression analyses in peach (Prunus persica) skin in response to different postharvest UV-B treatments. Plant Physiology and Biochemistry. 2018; 135 ():511-519.
Chicago/Turabian StyleMarco Santin; Luigi Lucini; Antonella Castagna; Gabriele Rocchetti; Marie-Theres Hauser; Annamaria Ranieri. 2018. "Comparative “phenol-omics” and gene expression analyses in peach (Prunus persica) skin in response to different postharvest UV-B treatments." Plant Physiology and Biochemistry 135, no. : 511-519.