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Abdullah A. Alyousef
Microbiology Research Group, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia

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Journal article
Published: 22 May 2020 in Nanomaterials
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Aminoglycosides (AMGs) have been extensively used to treat infectious diseases caused by Gram-negative bacteria in livestock and humans. A selective and sensitive colorimetric probe for the determination of streptomycin and kanamycin was proposed based on chlortetracycline-coated silver nanoparticles (AgNPs–CTC) as the sensing element. Almost all of the tested aminoglycoside antibiotics can rapidly induce the aggregation of AgNPs, along with a color change from yellow to orange/red. The selective detection of aminoglycoside antibiotics, including tobramycin, streptomycin, amikacin, gentamicin, neomycin, and kanamycin, with other types of antibiotics, can be achieved by ultraviolet (UV) spectroscopy. This developed colorimetric assay has ability to detect various AMGs using in-depth surface plasmon resonance (SPR) studies. With this determination of streptomycin and kanamycin was achieved at the picomolar level (pM) by using a UV–visible spectrophotometer. Under aqueous conditions, the linear range of the colorimetric sensor for streptomycin and kanamycin was 1000–1,1000 and 120–480 pM, respectively. The corresponding limit of detection was 2000 pM and 120 pM, respectively. Thus, the validated dual colorimetric and ratiometric method can find various analytical applications for the ultrasensitive and rapid detection of AMG antibiotics in water samples.

ACS Style

Ganesh Dattatraya Saratale; Rijuta Ganesh Saratale; Gajanan Ghodake; Surendra Shinde; Dae-Young Kim; Abdullah A. Alyousef; Mohammed Arshad; Asad Syed; Deepak Pant; Han-Seung Shin. Chlortetracycline-Functionalized Silver Nanoparticles as a Colorimetric Probe for Aminoglycosides: Ultrasensitive Determination of Kanamycin and Streptomycin. Nanomaterials 2020, 10, 997 .

AMA Style

Ganesh Dattatraya Saratale, Rijuta Ganesh Saratale, Gajanan Ghodake, Surendra Shinde, Dae-Young Kim, Abdullah A. Alyousef, Mohammed Arshad, Asad Syed, Deepak Pant, Han-Seung Shin. Chlortetracycline-Functionalized Silver Nanoparticles as a Colorimetric Probe for Aminoglycosides: Ultrasensitive Determination of Kanamycin and Streptomycin. Nanomaterials. 2020; 10 (5):997.

Chicago/Turabian Style

Ganesh Dattatraya Saratale; Rijuta Ganesh Saratale; Gajanan Ghodake; Surendra Shinde; Dae-Young Kim; Abdullah A. Alyousef; Mohammed Arshad; Asad Syed; Deepak Pant; Han-Seung Shin. 2020. "Chlortetracycline-Functionalized Silver Nanoparticles as a Colorimetric Probe for Aminoglycosides: Ultrasensitive Determination of Kanamycin and Streptomycin." Nanomaterials 10, no. 5: 997.

Journal article
Published: 26 December 2019 in Saudi Journal of Biological Sciences
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The present study explored the phytochemicals, antibacterial, antioxidant and cytotoxic effect of Tridax procumbens leaves. The leaves were dried and extracted with various organic solvents. The leaves contained the phytochemicals such as alkaloids, carbohydrates, polyphenols and tannins respectively. Antimicrobial potentials of the extracts were determined by performing the disc diffusion techniques. Results revealed that different organic solvents extracts namely methanol, ethanol and ethyl acetate extracts documented comparatively good activity against the studied microbial strains. The methanol extract of leaves of T. procumbens showed combatively better antioxidant potential. The tested plant leaf extract showed high activity against human lung cancer cells than breast cancer cell lines. 250 µg/ml plants extract showed 84 ± 2.8% toxicity against human lung cancer cells.

ACS Style

Asad Syed; Natarajan Benit; Abdullah A. Alyousef; Abdulaziz Alqasim; Mohammed Arshad. In-vitro antibacterial, antioxidant potentials and cytotoxic activity of the leaves of Tridax procumbens. Saudi Journal of Biological Sciences 2019, 27, 757 -761.

AMA Style

Asad Syed, Natarajan Benit, Abdullah A. Alyousef, Abdulaziz Alqasim, Mohammed Arshad. In-vitro antibacterial, antioxidant potentials and cytotoxic activity of the leaves of Tridax procumbens. Saudi Journal of Biological Sciences. 2019; 27 (2):757-761.

Chicago/Turabian Style

Asad Syed; Natarajan Benit; Abdullah A. Alyousef; Abdulaziz Alqasim; Mohammed Arshad. 2019. "In-vitro antibacterial, antioxidant potentials and cytotoxic activity of the leaves of Tridax procumbens." Saudi Journal of Biological Sciences 27, no. 2: 757-761.

Journal article
Published: 18 November 2019 in Processes
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In this current study, we demonstrated the green synthesis and characterization of silver nanoparticles using Myrtus communis L. plant extract (Ag-MC) and its evaluation of anticancer and antimicrobial activities. The green synthesis of (Ag-MC), was assessed by numerous characterization techniques such as ultraviolet-visible spectroscopy (UV-VIS), Fourier-transform infrared spectroscopy (FT-IR), X-ray powder diffraction (XRD) transmission electron microscopy (TEM) and energy dispersive x-ray spectroscopy (EDX). The anti-cancer activity of the green synthesized silver nanoparticles was evaluated by the median inhibitory dose (IC50) on human liver carcinoma cell lines (HepG2). These results suggested that SN-NPs can be used as effective anticancer cell lines, as well as antibacterial and antiseptic agents in the medical field. This study showed that overexpression of aldose reductase (AR) in the human liver carcinoma cell line, HepG2, was down regulated by administration of SN-MC. The down regulation of AR was associated with abrogation of Pl3k/Akt, ERK and NF-kB pathways and the inhibition of cancer hallmarks, however, the target molecule for SN-MC was not practically established. Thus it is still unknown if the consequences were due to AR inhibition or direct Ag-MC interaction with AR.

ACS Style

Abdulwahab Ali Abuderman; Rabbani Syed; Abdullah A. Alyousef; Mohammed S. Alqahtani; Mohammad Shamsul Ola; Abdul Malik. Green Synthesized Silver Nanoparticles of Myrtus communis L (AgMC) Extract Inhibits Cancer Hallmarks via Targeting Aldose Reductase (AR) and Associated Signaling Network. Processes 2019, 7, 860 .

AMA Style

Abdulwahab Ali Abuderman, Rabbani Syed, Abdullah A. Alyousef, Mohammed S. Alqahtani, Mohammad Shamsul Ola, Abdul Malik. Green Synthesized Silver Nanoparticles of Myrtus communis L (AgMC) Extract Inhibits Cancer Hallmarks via Targeting Aldose Reductase (AR) and Associated Signaling Network. Processes. 2019; 7 (11):860.

Chicago/Turabian Style

Abdulwahab Ali Abuderman; Rabbani Syed; Abdullah A. Alyousef; Mohammed S. Alqahtani; Mohammad Shamsul Ola; Abdul Malik. 2019. "Green Synthesized Silver Nanoparticles of Myrtus communis L (AgMC) Extract Inhibits Cancer Hallmarks via Targeting Aldose Reductase (AR) and Associated Signaling Network." Processes 7, no. 11: 860.

Preprint content
Published: 04 February 2019
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Poly-β-hydroxybutyrate (PHB) depolymerase can decompose biodegradable polymers and therefore has great commercial significance in the bioplastic sector. However, few reports have described PHB depolymerases based on isolates obtained from plastic-contaminated sites that reflect the potential of the source organism. In this study, we evaluated Microbacterium paraoxydans RZS6 as a producer of extracellular PHB depolymerase isolated from a plastic-contaminated site in the municipal area of Shahada, Maharashtra, India, for the first time. The isolate was identified using the polyphasic approach, i.e., 16S rRNA gene sequencing, gas chromatographic analysis of fatty acid methyl esters, and BIOLOG identification, and was found to hydrolyze PHB on minimal salt medium containing PHB as the only source of carbon. Both isolates produced PHB depolymerase at 30°C within 2 days and at 45°C within 4 days. The enzyme was purified most efficiently using an octyl-sepharose CL-4B column, with the highest purification yield of 6.675 U/mg/mL. The enzyme required Ce2+ and Mg2+ ions but was inhibited by Fe2+ ions and mercaptoethanol. Moreover, enzyme kinetic analysis revealed that the enzyme was a metalloenzyme requiring Mg2+ ions, with optimum enzyme activity at 45°C (thermophilic) and under neutrophilic conditions (optimum pH = 7). The presence of Fe2+ ions (1 mM) and mercaptoethanol (1000 ppm) completely inhibited the enzyme activity. The molecular weight of the enzyme (40 kDa), as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, closely resembled that of PHB depolymerase from Aureobacterium saperdae. Scale-up from the shake-flask level to a laboratory-scale bioreactor further enhanced the enzyme yield. Our findings highlighted the applicability of M. paraoxydans as a producer of extracellular PHB depolymerase isolated from a plastic-contaminated site in the municipal area of Shahada, Maharashtra, India.

ACS Style

Riyazali Zafarali Sayyed; Sj Wani; Abdullah A. Alyousef; Abdulaziz Alqasim; Asad Syed. Production, purification, and kinetics of the poly-β-hydroxybutyrate depolymerase from Microbacterium paraoxydans RZS6: A novel biopolymer-degrading organism isolated from a dumping yard. 2019, 540609 .

AMA Style

Riyazali Zafarali Sayyed, Sj Wani, Abdullah A. Alyousef, Abdulaziz Alqasim, Asad Syed. Production, purification, and kinetics of the poly-β-hydroxybutyrate depolymerase from Microbacterium paraoxydans RZS6: A novel biopolymer-degrading organism isolated from a dumping yard. . 2019; ():540609.

Chicago/Turabian Style

Riyazali Zafarali Sayyed; Sj Wani; Abdullah A. Alyousef; Abdulaziz Alqasim; Asad Syed. 2019. "Production, purification, and kinetics of the poly-β-hydroxybutyrate depolymerase from Microbacterium paraoxydans RZS6: A novel biopolymer-degrading organism isolated from a dumping yard." , no. : 540609.

Article
Published: 01 January 2019 in Biotechnology & Biotechnological Equipment
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Green fabrication of nanoparticles has been established by means of a simple, economical and environmentally pleasant process. The present investigation describes the use of Myrtus communis plant leaves extract for the biosynthesis of the silver nanoparticle. The surface plasmon resonance peak at 435 nm, distinctive for silver, determined the synthesis of AgNP. The morphology of the particles was spherical and in size with an average diameter of ∼ 15 nm. Energy dispersive X-ray Fourier Transformed Infrared and X-ray diffraction techniques investigated the crystalline structure, purity and functional groups. The biogenic nanoparticles revealed the antibacterial efficacy against both gram-positive bacteria and gram-negative bacteria, suggesting its prospective applicability as an effective antibacterial agent.

ACS Style

Abdullah A. Alyousef; Mohammed Arshad; Raid Alakeel; Abdulaziz Alqasim. Biogenic silver nanoparticles by Myrtus communis plant extract: biosynthesis, characterization and antibacterial activity. Biotechnology & Biotechnological Equipment 2019, 33, 931 -936.

AMA Style

Abdullah A. Alyousef, Mohammed Arshad, Raid Alakeel, Abdulaziz Alqasim. Biogenic silver nanoparticles by Myrtus communis plant extract: biosynthesis, characterization and antibacterial activity. Biotechnology & Biotechnological Equipment. 2019; 33 (1):931-936.

Chicago/Turabian Style

Abdullah A. Alyousef; Mohammed Arshad; Raid Alakeel; Abdulaziz Alqasim. 2019. "Biogenic silver nanoparticles by Myrtus communis plant extract: biosynthesis, characterization and antibacterial activity." Biotechnology & Biotechnological Equipment 33, no. 1: 931-936.

Journal article
Published: 03 April 2018 in BMC Complementary and Alternative Medicine
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Cucumis sativus L. (cucumber), from the family Cucurbitaceae, is a therapeutic plant with various pharmacological benefits, broadly utilized as a part of complementary medicine (e.g., Unani, Ayurveda, Siddha, and Traditional Chinese). In light of past research discoveries, this plant had been chosen to consider its potential antibacterial action. Extracts were purified by dialysis and ion exchange chromatography strategy and then assayed for antibacterial activity against four standard pathogenic bacterial strains known to cause foodborne infections and spoilage of food and herbal drugs. Antimicrobial peptides were extracted from seeds using a sodium phosphate citrate (pH 7.2) - CTAB cradle (pH 6.0). The highest protein concentration was seen with elute fractions 1 and 3 (370 mg/mL) compared with elute fractions 2 and 4 (340 mg/mL). Among the bacteria utilized, E. coli was clearly the most sensitive out of selected four strains. Our results suggest that Cucumis sativus L seeds extracts have significant potentials as new antimicrobial agents.

ACS Style

Raid Al Akeel; Ayesha Mateen; Khalid K. Alharbi; Abdullah A. Alyousef; Hazem M. Al-Mandeel; Rabbani Syed. Purification and MIC analysis of antimicrobial proteins from Cucumis sativus L. seeds. BMC Complementary and Alternative Medicine 2018, 18, 121 .

AMA Style

Raid Al Akeel, Ayesha Mateen, Khalid K. Alharbi, Abdullah A. Alyousef, Hazem M. Al-Mandeel, Rabbani Syed. Purification and MIC analysis of antimicrobial proteins from Cucumis sativus L. seeds. BMC Complementary and Alternative Medicine. 2018; 18 (1):121.

Chicago/Turabian Style

Raid Al Akeel; Ayesha Mateen; Khalid K. Alharbi; Abdullah A. Alyousef; Hazem M. Al-Mandeel; Rabbani Syed. 2018. "Purification and MIC analysis of antimicrobial proteins from Cucumis sativus L. seeds." BMC Complementary and Alternative Medicine 18, no. 1: 121.

Journal article
Published: 08 April 2017 in Molecules
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Foeniculum vulgare Mill., commonly called fennel, is a medicinal plant belonging to the Umbelliferae (Apiaceae) family, and is used in traditional medicine. Antibacterial peptides were isolated using sodium phosphate citrate buffer and, for extraction, cetyltrimethyl ammonium bromide (CTAB) buffer with pH 6, have been employed and antimicrobial activity tested against four reference strains. The extracted protein was subjected to 3 kDa dialysis and separation was carried out by DEAE-ion exchange chromatography and further proteins were identified by 2D gel electrophoresis. The results of Foeniculum vulgare elutes obtained from DEAE-ion exchange chromatography were tested for antibacterial activity. Elute 3 shows the highest antibacterial activity on Pseudomonas aeruginosa with a diameter of a zone of inhibition of 16 mm and IC50 value 25.02 (mcg/mL). Based on the findings of the wide usage in treatment of various ailments and day-to-day life, Foeniculum vulgare seeds were used in the present research and have shown promising antibacterial effects, which requires further proteomic research to authenticate the role of the anticipated proteins.

ACS Style

Raid Al Akeel; Ayesha Mateen; Rabbani Syed; Abdullah A. Alyousef; Mohammed Rafi Shaik. Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare. Molecules 2017, 22, 602 .

AMA Style

Raid Al Akeel, Ayesha Mateen, Rabbani Syed, Abdullah A. Alyousef, Mohammed Rafi Shaik. Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare. Molecules. 2017; 22 (4):602.

Chicago/Turabian Style

Raid Al Akeel; Ayesha Mateen; Rabbani Syed; Abdullah A. Alyousef; Mohammed Rafi Shaik. 2017. "Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare." Molecules 22, no. 4: 602.