Salinity stress is a severe environmental stress that affects plant growth and productivity of potato, a strategic crop moderately sensitive to saline soils. Limited studies are available on the use of combined nano-micronutrients to ameliorate salinity stress in potato plants (Solanum tuberosum L.). Two open field experiments were conducted in salt-affected sandy soil to investigate plant growth, physiology, and yield of potato in response to soil salinity stress under single or combined application of Zn, B, Si, and Zeolite nanoparticles. It was hypothesized that soil application of nanoparticles enhanced plant growth and yield by alleviating the adverse impact of soil salinity. In general, all the nano-treatments applications significantly increased plant height, shoot dry weight, number of stems per plant, leaf relative water content, leaf photosynthetic rate, leaf stomatal conductance, chlorophyll content, and tuber yield, as compared to the untreated control. Furthermore, soil application of these treatments increased the concentration of nutrients (N, P, K, Ca, Zn, and B) in plant tissues, leaf proline, and leaf gibberellic acid hormone (GA3) in addition to contents of protein, carbohydrates, and antioxidant enzymes (polyphenol oxidase (PPO) and peroxidase (POD) in tubers. Compared to other treatments, the combined application of nanoparticles showed the highest plant growth, physiological parameters, endogenous elements (N, P, K, Ca, Zn, and B) and the lowest concentration of leaf abscisic acid (ABA) and transpiration rate. The present findings suggest that soil addition of the aforementioned nanoparticles can be a promising approach to improving crop productivity in salt-affected soils.

Water stress is the most critical aspect restricting the development of agriculture in regions with scarce water resources, which requires enhancing irrigation water-saving strategies. The current work discusses the potential application of the plant-strengthening root endophyte Piriformospora indica against moderate (25% less irrigation water) and severe (50% less irrigation water) water stress in comparison to the optimum irrigation conditions of greenhouse cucumbers. P. indica improved growth, nutrient content, and photosynthesis apparatus under normal or water-stress conditions. On the other hand, moderate and severe water stress reduced yield up to 47% and 83%, respectively, in non-colonized cucumber plants, while up to 28 and 78%, respectively, in P. indica-colonized plants. In terms of water-use efficiency (WUE), P. indica improved the WUE of colonized cucumber plants grown under moderate (26 L/kg) or severe stress (73 L/kg) by supporting colonized plants in producing higher yield per unit volume of water consumed by the crop in comparison to non-colonized plants under the same level of moderate (43 L/kg) or severe (81 L/kg) water stress. Furthermore, P. indica increased the indole-3-acetic acid (IAA) content, activity levels of catalase (CAT) and peroxidase (POD) with an apparent clear reduction in the abscisic acid (ABA), ethylene, malondialdehyde (MDA), proline contents and stomatal closure compared to non-stressed plants under both water-stress levels. In addition, chlorophyll a, b, a + b contents were increased in the leaves of the colonized plants under water-stress conditions. This improvement in chlorophyll content could be correlated with a significant increment in the transcripts of chlorophyll biosynthesis genes (protochlorophyllide oxidoreductase [POR], chlorophyll a oxygenase [CAO]) and a reduction in the chlorophyll degradation genes (PPH, pheophorbide a oxygenase [PAO], and red chlorophyll catabolite reductase [RCCR]). In conclusion, P. indica has the potential to enhance the cucumber yield grown under moderate water stress rather than severe water stress by improving WUE and altering the activity levels of antioxidant enzymes and chlorophyll metabolism-related genes.