As one of the most widely used pesticides in agriculture, chlorothalonil can pose threat to soil ecosystems. Therefore, the impact of this substance on the development of microbiological and biochemical properties of the soil as well as on the growth of spring wheat was evaluated. The study was conducted with two soils (loamy sand with pH_KCl 5.6 and sandy loam with pH_KCl 7.00), to which fungicide was used in the following doses: 0.00, 0.166 (recommended dose), 1.660, and 16.60 mg kg⁻¹ dry matter of soil (DM of soil). In addition, we determined the effectiveness of fertilizing substances (Lignohumat Super and Bioilsa N 12.5) in the restoration of soil homeostasis and chlorothalonil degradation in the soil. Chlorothalonil caused modifications in the count and biological diversity of soil microorganisms. It stimulated the growth of heterotrophic bacteria and actinobacteria, and inhibited the growth of fungi. This pesticide was a potent inhibitor of dehydrogenase, catalase and acid phosphatase activities. It showed variable effects on urease and alkaline phosphatase. The fungicide also a reduction the yield of dry matter of the aboveground parts of spring wheat. It should, however, be noted that these changes in the soil environment occurred after the introduction of higher doses of chlorothalonil. The fertilizing substances used contributed to enhanced microbial and biochemical activities of soils, while they did not significantly affect plant yields. The Bioilsa N 12.5 preparation was effective in chlorothalonil degradation, while Lignohumat Super reduced the degradation rate of the tested fungicide. Based on the conducted experiment, an ecological risk assessment of chlorothalonil was made by estimating the changes occurring in the soil environment evaluated through the microbiological and biochemical analyses of the soil.

The aim of this study was to determine the effect of three active substances, diflufenican, mesosulfuron-methyl and iodosulfuron-methyl-sodium, applied in combination, on soil microbial counts, the structure of soil microbial communities, activity of soil enzymes and their resistance to the tested product, the biochemical indicator of soil fertility, and spring wheat yield. Soil samples with the granulometric composition of sandy loam with pH_KCl 7.0 were used in a pot experiment. The herbicide was applied to soil at seven doses: 0.057 (dose recommended by the manufacturer), 1.140, 2.280, 4.560, 9.120, 18.240 and 36.480 mg kg⁻¹ soil DM. Uncontaminated soil served as the control treatment. It was found that a mixture of the tested active substances increased the counts of total oligotrophic bacteria and spore-forming oligotrophic bacteria, organotrophic bacteria and actinomycetes, decreased the counts of Azotobacter and fungi, and modified the structure of soil microbial communities. The highest values of the colony development (CD) index and the ecophysiological (EP) index were observed in fungi and organotrophic bacteria, respectively. The herbicide applied in the recommended dose stimulated the activity of catalase, urease and acid phosphatase, but it had no effect on the activity of dehydrogenases, alkaline phosphatase, arylsulfatase and β-glucosidase. The highest dose of the analyzed substances (36.480 mg kg⁻¹) significantly inhibited the activity of dehydrogenases, acid phosphatase, alkaline phosphatase and arylsulfatase. The values of the biochemical soil fertility indicator (BA₂₁) decreased in response to high doses of the herbicide. Urease was most resistant and dehydrogenases were least resistant to soil contamination with a mixture of diflufenican + mesosulfuron-methyl + iodosulfuron-methyl-sodium. The analyzed herbicide had an adverse influence on spring wheat yield, and doses of 18.240 and 36.480 mg kg⁻¹ led to eventual death of plants.

The use of fungicides in crop protection still effectively eliminates fungal pathogens of plants. However, fungicides may dissipate to various elements of the environment and cause irreversible changes. Considering this problem, the aim of the presented study was to evaluate changes in soil biological activity in response to contamination with azoxystrobin. The study was carried out in the laboratory on samples of sandy loam with a pH of 7.0 in 1 Mol KCl dm⁻³. Soil samples were treated with azoxystrobin in one of four doses: 0.075 (dose recommended by the manufacturer), 2.250, 11.25 and 22.50 mg kg⁻¹ soil DM (dry matter of soil). The control soil sample did not contain fungicide. Bacteria were identified based on 16S rRNA gene sequencing, and fungi were identified by internal transcribed spacer (ITS) region sequencing. The study revealed that increased doses of azoxystrobin inhibited the growth of organotrophic bacteria, actinomycetes and fungi. The fungicide also caused changes in microbial biodiversity. The lowest values of the colony development (CD) index were recorded for fungi and the ecophysiological (EP) index for organotrophic bacteria. Azoxystrobin had an inhibitory effect on the activity of dehydrogenases, catalase, urease, acid phosphatase and alkaline phosphatase. Dehydrogenases were found to be most resistant to the effects of the fungicide, while alkaline phosphatase in the soil recovered the balance in the shortest time. Four species of bacteria from the genus Bacillus and two species of fungi from the genus Aphanoascus were isolated from the soil contaminated with the highest dose of azoxystrobin (22.50 mg kg⁻¹).

The research objective has been to evaluate the effect, unexplored yet, of a mixture of three active ingredients of the herbicide Lumax 537.5 SE: terbuthylazine (T), mesotrione (M), and S-metolachlor (S) on counts of soil microorganisms, structure of microbial communities, activity of soil enzymes as well as the growth and development of maize. The research was based on a pot experiment established on sandy soil with pH_KCl 7.0. The herbicide was applied to soil once, in the form of liquid emulsion dosed as follows: 0.67, 13.4, 26.9, 53.8, 108, 215, and 430 mg kg⁻¹ of soil, converted per active substance (M + T + S). The control sample consisted of soil untreated with herbicide. The results showed that the mixture of the above active substances caused changes in values of the colony development (CD) indices of organotrophic bacteria, actinomycetes, and fungi and ecophysiological diversity (EP) indices of fungi. Changes in the ecophysiological diversity index of organotrophic bacteria and actinomycetes were small. The M + T + S mixture was a strong inhibitor of dehydrogenases, to a less degree catalase, urease, β-glucosidase, and arylsulfatase, while being a weak inhibitor of phosphatases. The actual impact was correlated with the dosage. The M + T + S mixture inhibited the growth and development of maize. The herbicide Lumax 537.5 SE should be applied strictly in line with the regime that defines its optimum dosage. Should its application adhere to the manufacturer’s instructions, the herbicide would not cause any serious disturbance in soil homeostasis. However, its excessive quantities (from 13.442 to 430.144 mg kg⁻¹ DM of soil) proved to be harmful to the soil environment.

Fungicides are considered to be effective crop protection chemicals in modern agriculture. However, they can also exert toxic effects on non-target organisms, including soil-dwelling microbes. Therefore, the environmental fate of fungicides has to be closely monitored. The aim of this study was to evaluate the influence of the Falcon 460 EC fungicide on microbial diversity, enzyme activity and resistance, and plant growth. Samples of sandy loam with pH_KCl 7.0 were collected for laboratory analyses on experimental days 30, 60 and 90. Falcon 460 EC was applied to soil in the following doses: control (soil without the fungicide), dose recommended by the manufacturer, 30-fold higher than the recommended dose, 150-fold higher than the recommended dose and 300-fold higher than the recommended dose. The observed differences in the values of the colony development index and the eco-physiological index indicate that the mixture of spiroxamine, tebuconazole and triadimenol modified the biological diversity of the analyzed groups of soil microorganisms. Bacteria of the genus Bacillus and fungi of the genera Penicillium and Rhizopus were isolated from fungicide-contaminated soil. The tested fungicide inhibited the activity of dehydrogenases, catalase, urease, acid phosphatase and alkaline phosphatase. The greatest changes were induced by the highest fungicide dose 300-fold higher than the recommended dose. Dehydrogenases were most resistant to soil contamination. The Phytotoxkit test revealed that the analyzed fungicide inhibits seed germination capacity and root elongation. The results of this study indicate that excessive doses of the Falcon 460 EC fungicide 30-fold higher than the recommended dose to 300-fold higher than the recommended dose) can induce changes in the biological activity of soil. The analyzed microbiological and biochemical parameters are reliable indicators of the fungicide’s toxic effects on soil quality.