The functional properties of chicken wooden breast myofibrillar protein (WBMP) are impaired. The protein structure and functional properties of WBMP are investigated using high-intensity ultrasound (HIU, 20 kHz, 200, 400, 600, and 800 W) combined with pH-shifting. HIU promoted the unfolding of WBMP, reduced the particle size of WBMP, and enhanced electrostatic repulsion. Medium-power (200 and 400 W) HIU promoted the α-helix to β-sheet transformation, while high-power (600 and 800 W) HIU significantly (P < 0.05) increased the content of the random coil. The microstructure and images after storage further showed that 400 W HIU in combination with pH-shifting made the WBMP emulsion more uniform. In addition, gel performance analysis showed that the gel strength and water-holding capacity of the protein gel increased gradually after 400 W. Scanning electron microscope images also showed the formation of a stable network structure in the protein gel. This work could help promote the utilization of inferior proteins similar to WBMP, but the utilization rate still needs to be further improved.

• Heteropolysaccharides are more suitable for addition to emulsions than other sugars. • The electrostatic repulsion hinders the stratification of the emulsion. • The protein membrane in the emulsion is affected by different sugar structures. • Heteropolysaccharides make emulsions more stable during long-term storage.

The deterioration of wooden breast myofibrillar protein (WBMP) causes a decline in its processing performance, and the protein becomes easier to oxidize. Previous studies have revealed that the use of (-)-epigallocatechin-3-gallate (EGCG) may improve the physicochemical properties and oxidative stability of proteins in aqueous solutions. The effects of varying concentrations (0.01%, 0.02%, 0.03%, and 0.04% w/v) of EGCG on the physicochemical properties of a WBMP emulsion (1.2% WBMP/10% oil) and the inhibition of lipid and protein oxidation were studied. The results revealed that a moderate dose of EGCG (0.03%) could significantly (p < 0.05) improve the emulsion activity index (4.66 ± 0.41 m²/g) and emulsion stability index (91.95 ± 4.23%), as well as reduce the particle size of the WBMP emulsion. According to the micrographs and cream index, 0.03% EGCG retarded the phase separation by stopping the aggregation of droplets and proteins, thus significantly improving the stability of WBMP emulsions. During storage at 50 °C for 96 h, 0.03% EGCG inhibited lipid oxidation (lipid hydroperoxide and 2-thiobarbituric acid-reactive substance formation) and protein oxidation (carbonyl formation and sulfhydryl loss). In contrast, lower and higher EGCG concentrations (0.01%, 0.02%, and 0.04%) demonstrated shortcomings (such as weak antioxidant capacity or protein over-aggregation) in improving the quality and oxidation stability of the emulsion. In conclusion, a moderate dose of EGCG (0.03%) can be used to improve the quality and shelf life of WBMP emulsions.

The present study focused on the investigation of yak ( Bos grunniens ) meat peptides on immunity, antioxidant capacity, and hypoxia tolerance in hypotonic hypoxic mice using malondialdehyde (MDA), superoxide dismutase (SOD) kit, and so on. Yak protein powder was extracted first, and then peptides were obtained by INFOGEST static simulation of human gastrointestinal tract digestion. The results demonstrated that yak meat peptides increased the content of immune cells, oxygen transport capacity, and antioxidant enzyme activities in mice, while reducing oxidative stress damage in the body. In addition, protein expression of hypoxia‐related genes hypoxia‐inducible factor (HIF) and erythropoietin (EPO) was improved. This study can provide a theoretical foundation for reducing the harm of reactive oxygen species and free radicals to the human body and for the development and utilization of yak bioactive substances.