Fluorescence Resonance Energy Transfer (FRET) has emerged as a predominant, highly sensitive, and homogeneous optical analytical technique in the realm of analytical testing and bio-imaging. Gold nanoparticles (AuNPs) demonstrate a size-dependent, broader absorption range within visible wavelengths owing to the phenomenon of surface plasmon resonance. As a result, they can effectively act as light acceptors, enabling the creation of a donor-acceptor system crucial for achieving precise target analyte analysis. In this comprehensive review, we present an extensive survey of recent research advancements in the field of FRET techniques based on AuNPs for the analytical detection of a wide range of entities, including some biomolecules, pesticides, enzymes, microorganisms, food safety and environmental pollutants. Additionally, we elucidate the procedural strategies and underlying mechanisms involved. Finally, we provide perspectives on the current issues and future efforts surrounding the FRET applications of AuNPs in biological analysis. Overall, this review aims to provide a holistic comprehension of gold nanoparticle applications in life analysis using FRET, while also presenting a promising vision for future endeavors in this domain.

Biosensor analysis based on the surface plasmon resonance (SPR) phenomenon enables label-free, highly sensitive analyte detection without prior sample purification or processing. However, potential applications of SPR biosensors in virus detection in biological samples remain to be explored. Owing to its excellent biocompatibility and abundance of hydroxyl and carboxyl functional groups, graphene oxide (GO) has been widely used as a biosensor of proteins and metal ions in living cells. The present work explored the effect of GO modification on the sensitivity of an SPR biosensor and used a GO-modified sensor to detect porcine reproductive and respiratory syndrome virus in cell culture, as shown. The GO modification markedly enhanced the sensitivity of the Fourier transform SPR sensor and enabled linear detection of porcine reproductive and respiratory syndrome virus (PRRSV) with a multiplicity of infection in the range 0.2–1.7 (R² = 0.998). Such a GO-modified sensor provides a promising alternative for virus detection.

Tumor invasion/metastasis is still the major cause of death in cancer patients. Membrane type-1 matrix metalloproteinase (MT1-MMP) is directly related to tumor invasion/metastasis. To accurately and quickly distinguish the risk of invasion/metastasis of primary tumor cells, it is urgent to develop a simple and precise quantitative method to distinguish the expression level of MT1-MMP. In this work, we have constructed red fluorescent Au clusters with peroxidase-like properties that could specifically bind to MT1-MMP on human cervical cancer cells. After MT1-MMP was labelled with Au clusters, we could visually see red fluorescence of MT1-MMP on cervical cancer cells via fluorescence microscopy and catalytic color imaging using an ordinary optical microscope. The constructed Au clusters contained 26 Au atoms; thus, the amount of MT1-MMP on cervical cancer cells could be accurately quantified using inductively coupled plasma mass spectrometry (ICP-MS). More importantly, the invasion/metastasis capabilities of the cervical cancer Siha, Caski and Hela cells with different MT1-MMP amounts could be accurately distinguished by fluorescence/catalysis qualitative imaging and ICP-MS quantitative analysis. This method of qualitative/quantitative analysis of tumor-associated proteins on cancer cells has great potential for accurately diagnosing aggressive tumor cells and assessment of their invasion/metastasis risk.