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Exopolysaccharides, or extracellular polysaccharides (EPS, sPS), represent a valuable metabolite compound synthesized from red microalgae. It is a non-toxic natural agent and can be applied as an immunostimulant. The toxicity test of exopolysaccharides from Porphyridium has been done in vivo using zebrafish (Danio rerio) embryonic model, or the ZET (zebrafish embryotoxicity test). The administration of extracellular polysaccharides or exopolysaccharides (EPS) from microalgae Porphyridium cruentum (synonym: P. purpureum) to shrimps Litopenaeus vannamei was investigated to determine the effect of this immunostimulant on their non-specific immune response and to test if this compound can be used as a protective agent for shrimps in relation to Vibrio infection. For immune response, exopolysaccharides were given to shrimps via the immersion method on day 1 and booster on day 8. Shrimp hemocytes were taken on day 1 (EPS administration), day 7 (no treatment), day 8 (EPS booster) and day 9 (Vibrio infection) and tested for their immune response on each treatment. The result shows that the EPS is not toxic, as represented by the normal embryonic development and the mortality data. In the Pacific white shrimps, an increase in the values of all immune parameters was shown, in line with the increasing EPS concentration, except for the differential hemocyte count (DHC). In detail, an increase was noted in total hemocytes (THC) value, phagocytotic activity (PA) and respiratory burst (RB) in line with the EPS concentration increase. These results and other previous studies indicate that EPS from Porphyridium is safe, enhances immune parameters in shrimp rapidly, and has the ability to act as an immunostimulant or an immunomodulator. It is a good modulator for the non-specific immune cells of Pacific white shrimps, and it can be used as a preventive agent against vibriosis.
Yenny Risjani; Nurul Mutmainnah; Praprianita Manurung; Siti Wulan; Yunianta. Exopolysaccharide from Porphyridium cruentum (purpureum) is Not Toxic and Stimulates Immune Response against Vibriosis: The Assessment Using Zebrafish and White Shrimp Litopenaeus vannamei. Marine Drugs 2021, 19, 133 .
AMA StyleYenny Risjani, Nurul Mutmainnah, Praprianita Manurung, Siti Wulan, Yunianta. Exopolysaccharide from Porphyridium cruentum (purpureum) is Not Toxic and Stimulates Immune Response against Vibriosis: The Assessment Using Zebrafish and White Shrimp Litopenaeus vannamei. Marine Drugs. 2021; 19 (3):133.
Chicago/Turabian StyleYenny Risjani; Nurul Mutmainnah; Praprianita Manurung; Siti Wulan; Yunianta. 2021. "Exopolysaccharide from Porphyridium cruentum (purpureum) is Not Toxic and Stimulates Immune Response against Vibriosis: The Assessment Using Zebrafish and White Shrimp Litopenaeus vannamei." Marine Drugs 19, no. 3: 133.
Exopolysaccharides or extracellular polysaccharides (EPS, sPS) represent valuable metabolite compound synthesized from red microalgae. It is a non toxic natural agent and can be applied as immunostimulant. Toxicity test of exopolysaccharides from Porphyridium has been done in-vivo using zebrafish (Danio rerio) embryonic model, or the ZET (Zebrafish Embryotoxicity Test). The administration of extracellular polysaccharide or exopolysaccharides (EPS) from microalgae Porphyridium cruentum (synonym: P. purpureum) on shrimps Litopenaeus vannamei was investigated to determine the effect of this immunostimulant on their non specific immune response and to test if this compound can be used as a protective agent for shrimp related to Vibrio infection. For immune response, exopolysaccharides was given to shrimps by immersion method on day 1 and booster on day 8. Shrimp hemocytes were taken on day 1 (EPS administration), day 7 (no treatment), day 8 (EPS booster) and day 9 (Vibrio infection) and tested for their immune response on each treatment. Result shows that the EPS is not toxic as represented by the normal embryonic development and the mortality data. In the Pacific whiteshrimps, it show an increase in values of all immune parameters in line with the increasing EPS concentration, except the Differential Haemocyte Count (DHC). In detail, an increase was noted in total hemocytes (THC) value, Phagocytotic Activity (PA), Respiratory Burst (RB) in line with the EPS concentration increase. These results and other previous studies indicate that EPS from Porphyridium is safe and it enhances immune parameters in shrimp rapidly and has the ability as an immunostimulant or an immunomodulator. It is a good modulator for the non-specific immune cells of Pacific white shrimps, and it can be used as a preventive agent against vibriosis.
Yenny Risjani; Nurul Mutmainnah; Praprianita Manurung; Siti Wulan; Yunianta Yunianta. Exopolysaccharide from Porphyridium cruentum (purpureum) is Not Toxic and Stimulates Immune Response against Vibriosis: The Assessment Using Zebrafish and White Shrimp Litopenaeus vannamei. 2021, 1 .
AMA StyleYenny Risjani, Nurul Mutmainnah, Praprianita Manurung, Siti Wulan, Yunianta Yunianta. Exopolysaccharide from Porphyridium cruentum (purpureum) is Not Toxic and Stimulates Immune Response against Vibriosis: The Assessment Using Zebrafish and White Shrimp Litopenaeus vannamei. . 2021; ():1.
Chicago/Turabian StyleYenny Risjani; Nurul Mutmainnah; Praprianita Manurung; Siti Wulan; Yunianta Yunianta. 2021. "Exopolysaccharide from Porphyridium cruentum (purpureum) is Not Toxic and Stimulates Immune Response against Vibriosis: The Assessment Using Zebrafish and White Shrimp Litopenaeus vannamei." , no. : 1.
White shrimps are susceptible to outbreaks of vibriosis because they do not have any adaptive immune system, they only have a non-specific innate immune system. The administration of EPS from microalgae Porphyridium cruentum (synonym: P. purpureum) on shrimps Litopenaeus vannamei was investigated to determine the effect of this immunostimulant on their non specific immune response and to test if EPS can be used as a protective agent for shrimp related to Vibrio infection. EPS was given to shrimps by immersion method on day 1 and booster on day 8. Shrimp hemocytes were taken on day 1 (EPS administration), day 7 (no treatment), day 8 (EPS booster) and day 9 (Vibrio infection) and tested for their immune response on each treatment. Result shows an increase in values of all immune parameters in line with the increasing EPS concentration, except the Differential Haemocyte Count (DHC). In detail, an increase was noted in total hemocytes (THC) value, Phagocytotic Activity (PA), Respiratory Burst (RB) in line as the EPS concentration increase. Although there is a decrease after the infection, the value obtained is not lower than the control value. These results indicate that EPS from Porphyrydium enhances immune parameters in shrimp rapidly and has the ability as an immunostimulant or an immunomodulator. It is a good modulator for the non specific immune cells of Pacific white shrimps, and it can be used as a preventive agent against Vibrio.
Yenny Risjani; Nurul Mutmainnah; Siti Narsito Wulan; Yunianta Yunianta. Extracellular Polysaccharide from Porphyridium cruentum (purpureum) Stimulates the Non-Specific Immune Activities on White Shrimp (Litopenaeus vannamei). 2020, 1 .
AMA StyleYenny Risjani, Nurul Mutmainnah, Siti Narsito Wulan, Yunianta Yunianta. Extracellular Polysaccharide from Porphyridium cruentum (purpureum) Stimulates the Non-Specific Immune Activities on White Shrimp (Litopenaeus vannamei). . 2020; ():1.
Chicago/Turabian StyleYenny Risjani; Nurul Mutmainnah; Siti Narsito Wulan; Yunianta Yunianta. 2020. "Extracellular Polysaccharide from Porphyridium cruentum (purpureum) Stimulates the Non-Specific Immune Activities on White Shrimp (Litopenaeus vannamei)." , no. : 1.
The taxonomy and identification of carrageenophyte red marine algae is confused due to lack of adequate characteristics. There are also difficulties when analyzing the species, relationship between color morphotype and site of origin. Meanwhile, the use of molecular tool has been promising in identifying the similarity and genetic gap between the varieties from different location. In fact, it is useful in seaweed selection to obtain superior heredity. Genetic resources diversity provides opportunity for breeders to obtain new strains or improved varieties with more desirable characteristics. Therefore, this study aimed to compare the similarities between the green and brown morphotypes of Kappaphycus alvarezii that inhabit different sites in Indonesia. The samples were obtained from Pinrang, Lombok, and Madura and 5 primer genomes of PCR were used for DNA isolation. This was achieved by incorporating the modified phenol-chloroform method of random amplified polymorphic DNA (RAPD). The data were processed in the RAPD instance package version 1.04 supported by UPGMA (unweighted pair group method of arithmetic). These are in one package with sequential, agglomerative, hierarchical, and nested clustering (SHAN), and used to obtain data grouping based on their genetic gap. Also, analysis of the genetic relationship of K. alvarezii originating from different locations shows high gap and variation. Therefore, it was concluded that seaweeds based on their cultivation origin have higher genetic gap than those based on color. For the purpose of breeding, selection should be based on the location rather than color. Furthermore, this study showed that RAPD is effective in identifying variety differences.
Yenny Risjani; Gunawan Abidin. Genetic diversity and similarity between green and brown morphotypes of Kappaphycus alvarezii using RAPD. Environmental Biology of Fishes 2020, 32, 2253 -2260.
AMA StyleYenny Risjani, Gunawan Abidin. Genetic diversity and similarity between green and brown morphotypes of Kappaphycus alvarezii using RAPD. Environmental Biology of Fishes. 2020; 32 (4):2253-2260.
Chicago/Turabian StyleYenny Risjani; Gunawan Abidin. 2020. "Genetic diversity and similarity between green and brown morphotypes of Kappaphycus alvarezii using RAPD." Environmental Biology of Fishes 32, no. 4: 2253-2260.
Yenny Risjani; D R Santoso; J Couteau; A Hermawati; I Widowati; C Minier. Impact of anthropogenic activity and lusi-mud volcano on fish biodiversity at the Brantas Delta, Indonesia. IOP Conference Series: Earth and Environmental Science 2020, 493, 1 .
AMA StyleYenny Risjani, D R Santoso, J Couteau, A Hermawati, I Widowati, C Minier. Impact of anthropogenic activity and lusi-mud volcano on fish biodiversity at the Brantas Delta, Indonesia. IOP Conference Series: Earth and Environmental Science. 2020; 493 ():1.
Chicago/Turabian StyleYenny Risjani; D R Santoso; J Couteau; A Hermawati; I Widowati; C Minier. 2020. "Impact of anthropogenic activity and lusi-mud volcano on fish biodiversity at the Brantas Delta, Indonesia." IOP Conference Series: Earth and Environmental Science 493, no. : 1.
Aeromonas hydrophila often attacks cultured catfish and causes motile aeromonad septicemia (MAS) disease outbreak in South Kalimantan, Indonesia. Deaths from A.hydrophila attacks could reach 100% so that prevention needs to done through vaccination. This study aimed to examine the potential immunogenicity of 6 heat-killed A.hydrophila vaccine candidates, a strain of Banjar, South Kalimantan, Indonesia. A.hydrophila strains obtained from infected catfish in aquaculture ponds around the Banjar District, South Kalimantan, Indonesia. From 10 fish infected with MAS, obtained 14 isolates of bacteria, ie, 8 isolates (AGC-1, AGC-2, AGC-3, AGC-4, AGC-6, AKC-2, AKC-3, and AKC-5) of Sungai Batang village, and 6 isolates (AGC-8, AGC-9, AKC-7, AKC-8, AKC-9, AKC-10) from Cindai Alus village. AGC signifies Aeromonas isolated from the gills, and AKC means Aeromonas isolated from the kidney. The antigen that used as a candidate for the heat-killed A.hydrophila vaccine made by inactivation through a heating process at 100 oC for 60 minutes. The results showed antigens from AGC-2 and AGC-8 strains had high immunogenicity because they could increase antibody titers compared to other strains and controls. The antibody titer in catfish, two weeks after booster vaccination, increased and showed the same value. The results of the cross-reaction assay showed that the antigens from the AGC-2 and AGC-8 strains were able to cross-react with strain AGC-1, AKC3, AKC-5, but unable to cross-react with AKC-7, so that AGC-2 and AGC-8 could be recommended as vaccine candidates for MAS disease in South Kalimantan, Indonesia.
Olga Olga; Siti Aisiah; Wendy Alexander Tanod; Yenny Risjani; Happy Nursyam; Maftuch Maftuch. Immunogenization of Heat-Killed Vaccine Candidate from Aeromonas hydrophila in Catfish (Pangasius hypophthalamus) using Strain of Banjar, South Kalimantan, Indonesia. Egyptian Journal of Aquatic Biology and Fisheries 2020, 24, 1 -13.
AMA StyleOlga Olga, Siti Aisiah, Wendy Alexander Tanod, Yenny Risjani, Happy Nursyam, Maftuch Maftuch. Immunogenization of Heat-Killed Vaccine Candidate from Aeromonas hydrophila in Catfish (Pangasius hypophthalamus) using Strain of Banjar, South Kalimantan, Indonesia. Egyptian Journal of Aquatic Biology and Fisheries. 2020; 24 (4):1-13.
Chicago/Turabian StyleOlga Olga; Siti Aisiah; Wendy Alexander Tanod; Yenny Risjani; Happy Nursyam; Maftuch Maftuch. 2020. "Immunogenization of Heat-Killed Vaccine Candidate from Aeromonas hydrophila in Catfish (Pangasius hypophthalamus) using Strain of Banjar, South Kalimantan, Indonesia." Egyptian Journal of Aquatic Biology and Fisheries 24, no. 4: 1-13.
Environmental and human health challenges are pronounced in Asia, an exceptionally diverse and complex region where global megatrends are extensive and numerous stresses to environmental quality exist. Identifying priorities necessary to engage grand challenges can be facilitated through horizon scanning exercises, and to this end we identified and examined 23 priority research questions needed to advance toward more sustainable environmental quality in Asia, as part of the Global Horizon Scanning Project. Advances in environmental toxicology, environmental chemistry, biological monitoring and risk assessment methodologies are necessary to address adverse impact of environmental stressors on ecosystem services and biodiversity, for which Asia is home to numerous biodiversity hotspots. Intersections of the food‐energy‐water nexus are profound in Asia; innovative and aggressive technologies are necessary to provide clean water, ensure food safety, and stimulate energy efficiency, while improving ecological integrity and addressing legacy and emerging threats to public health and the environment, particularly with increased aquaculture production. Asia is the largest chemical producing continent globally. Accordingly, sustainable and green chemistry and engineering presents decided opportunities to stimulate innovation and realize a number of the United Nations Sustainable Development Goals. Engaging the priority research questions identified herein will require transdisciplinary coordination through existing and non‐traditional partnerships within and among countries and sectors. Answering these questions will not be easy, but is necessary to achieve more sustainable environmental quality in Asia. This article is protected by copyright. All rights reserved.
Kenneth M.Y. Leung; Katie W. Y. Yeung; Jing You; Kyungho Choi; Xiaowei Zhang; Ross Smith; Guang‐Jie Zhou; Mana M.N. Yung; Carlos Arias‐Barreiro; Youn‐Joo An; S. Rebekah Burket; Robert Dwyer; Nathalie Goodkin; Yii Siang Hii; Tham Hoang; Chris Humphrey; Chuleemas Boonthai Iwai; Seung‐Woo Jeong; Guillaume Juhel; Ali Karami; Katerina Kyriazi‐Huber; Kuan‐Chun Lee; Bin‐Le Lin; Ben Lu; Patrick Martin; Mae Grace Nillos; Katharina Oginawati; I.V.N. Rathnayake; Yenny Risjani; Mohammad Shoeb; Chin Hon Tan; Maria Claret Tsuchiya; Gerald T. Ankley; Alistair B. A. Boxall; Murray A. Rudd; Bryan W. Brooks. Toward Sustainable Environmental Quality: Priority Research Questions for Asia. Environmental Toxicology and Chemistry 2020, 39, 1485 -1505.
AMA StyleKenneth M.Y. Leung, Katie W. Y. Yeung, Jing You, Kyungho Choi, Xiaowei Zhang, Ross Smith, Guang‐Jie Zhou, Mana M.N. Yung, Carlos Arias‐Barreiro, Youn‐Joo An, S. Rebekah Burket, Robert Dwyer, Nathalie Goodkin, Yii Siang Hii, Tham Hoang, Chris Humphrey, Chuleemas Boonthai Iwai, Seung‐Woo Jeong, Guillaume Juhel, Ali Karami, Katerina Kyriazi‐Huber, Kuan‐Chun Lee, Bin‐Le Lin, Ben Lu, Patrick Martin, Mae Grace Nillos, Katharina Oginawati, I.V.N. Rathnayake, Yenny Risjani, Mohammad Shoeb, Chin Hon Tan, Maria Claret Tsuchiya, Gerald T. Ankley, Alistair B. A. Boxall, Murray A. Rudd, Bryan W. Brooks. Toward Sustainable Environmental Quality: Priority Research Questions for Asia. Environmental Toxicology and Chemistry. 2020; 39 (8):1485-1505.
Chicago/Turabian StyleKenneth M.Y. Leung; Katie W. Y. Yeung; Jing You; Kyungho Choi; Xiaowei Zhang; Ross Smith; Guang‐Jie Zhou; Mana M.N. Yung; Carlos Arias‐Barreiro; Youn‐Joo An; S. Rebekah Burket; Robert Dwyer; Nathalie Goodkin; Yii Siang Hii; Tham Hoang; Chris Humphrey; Chuleemas Boonthai Iwai; Seung‐Woo Jeong; Guillaume Juhel; Ali Karami; Katerina Kyriazi‐Huber; Kuan‐Chun Lee; Bin‐Le Lin; Ben Lu; Patrick Martin; Mae Grace Nillos; Katharina Oginawati; I.V.N. Rathnayake; Yenny Risjani; Mohammad Shoeb; Chin Hon Tan; Maria Claret Tsuchiya; Gerald T. Ankley; Alistair B. A. Boxall; Murray A. Rudd; Bryan W. Brooks. 2020. "Toward Sustainable Environmental Quality: Priority Research Questions for Asia." Environmental Toxicology and Chemistry 39, no. 8: 1485-1505.
Hepcidin antimicrobial peptides are difficult to produce in prokaryotic expression systems due to their complex structure and antimicrobial activity. Although synthetic hepcidin provides an alternative to solve this issue, its high cost limits its practical application in various industries. The present study used zebrafish eggs as bioreactors to produce convict cichlid (Amatitlania nigrofasciata) hepcidin (AN-hepc) using the oocyte-specific zona pellucida (zp3) promoter. The expression plasmid pT2-ZP3-AN-hepc-ZP3-EGFP, using EGFP as a reporter of AN-hepc expression, was designed to establish the transgenic line Tg(ZP3:AN-hepc:ZP3:EGFP) for the expression of AN-hepc. The AN-hepc peptide was produced successfully in fertilized eggs, as evidenced by RT-PCR and Western blotting. The AN-hepc-expressing eggs exhibited antimicrobial activity against a variety of aquatic pathogens and antibiotic-resistant pathogens, suggesting that the AN-hepc expressed in fish eggs was bioactive. The immunomodulatory effects of AN-hepc-expressing fertilized eggs on zebrafish innate immunity were evaluated by determining the expression of indicator genes after feeding with AN-hepc-expressing fertilized eggs for two months. Zebrafish supplementation with AN-hepc-expressing fertilized eggs significantly increased the expression of innate immunity-related genes, including IL-1β, IL-6, IL-15, TNF-α, NF-κb, complement C3b, lysozyme and TLR-4a. The zebrafish administered AN-hepc-expressing eggs exhibited higher cumulative survival than fish supplemented with wild-type and control eggs after infection with Aeromonas hydrophila and Streptococcus iniae. In conclusion, the present results showed that supplementation with AN-hepc-expressing fish eggs enhanced zebrafish innate immunity against pathogenic infections, suggesting that fertilized eggs containing AN-hepc have the potential to be developed as a food supplement for improving health status in aquaculture.
Chung-Chih Tseng; Tah-Wei Chu; Ridha Danata; Yenny Risjani; Hui-Tsu Shih; Shao-Yang Hu. Hepcidin-Expressing Fish Eggs as A Novel Food Supplement to Modulate Immunity against Pathogenic Infection in Zebrafish (Danio rerio). Sustainability 2020, 12, 1 .
AMA StyleChung-Chih Tseng, Tah-Wei Chu, Ridha Danata, Yenny Risjani, Hui-Tsu Shih, Shao-Yang Hu. Hepcidin-Expressing Fish Eggs as A Novel Food Supplement to Modulate Immunity against Pathogenic Infection in Zebrafish (Danio rerio). Sustainability. 2020; 12 (10):1.
Chicago/Turabian StyleChung-Chih Tseng; Tah-Wei Chu; Ridha Danata; Yenny Risjani; Hui-Tsu Shih; Shao-Yang Hu. 2020. "Hepcidin-Expressing Fish Eggs as A Novel Food Supplement to Modulate Immunity against Pathogenic Infection in Zebrafish (Danio rerio)." Sustainability 12, no. 10: 1.
This paper reports the first data from an integrated study investigating genotoxicity in the Brantas River, Java, Indonesia. Results showed that organic sediment extracts from the sites in the Brantas Delta retained genotoxic compounds identified using the SOS Chromotest and that the Aloo River and, to a lesser extent, the Surabaya River were the most contaminated studied sites. This genotoxicity was attributable to compounds that did not require any bioactivation under the test conditions. Occurrence of genotoxic effects was further investigated in erythrocytes from Nile tilapia, Oreochromis niloticus. High numbers of micronuclei were counted, especially in fish sampled in the rivers of the Brantas Delta. Moreover, cytoplasmic alterations which could be indicative of the presence of lipofuscin were found in the cytoplasm of the fish blood cells, especially in fish from the Aloo, Surabaya and Kalimas rivers. Altogether, our data showed that genotoxicity is occurring in fish living in rivers of the delta of the Brantas River and suggest that sediments from these sites may constitute a major source of pollution and hazard for species living or feeding in the area.
Yenny Risjani; Géraldine Loppion; Jérôme Couteau; Yunianta Yunianta; Ita Widowati; Alfi Hermawati; Christophe Minier. Genotoxicity in the rivers from the Brantas catchment (East Java, Indonesia): occurrence in sediments and effects in Oreochromis niloticus (Linnæus 1758). Environmental Science and Pollution Research 2020, 27, 21905 -21913.
AMA StyleYenny Risjani, Géraldine Loppion, Jérôme Couteau, Yunianta Yunianta, Ita Widowati, Alfi Hermawati, Christophe Minier. Genotoxicity in the rivers from the Brantas catchment (East Java, Indonesia): occurrence in sediments and effects in Oreochromis niloticus (Linnæus 1758). Environmental Science and Pollution Research. 2020; 27 (17):21905-21913.
Chicago/Turabian StyleYenny Risjani; Géraldine Loppion; Jérôme Couteau; Yunianta Yunianta; Ita Widowati; Alfi Hermawati; Christophe Minier. 2020. "Genotoxicity in the rivers from the Brantas catchment (East Java, Indonesia): occurrence in sediments and effects in Oreochromis niloticus (Linnæus 1758)." Environmental Science and Pollution Research 27, no. 17: 21905-21913.
One of the wide spread aquatic organisms in the rivers of Indonesia is the mosquito fish (Gambusia affinis (Baird and Girard, 1853)). Mosquito fish are invasive species that can adapt to environmental changes, also they act as bioindicator species for polluted environments. The study aims to determine the effects of the exposure to cadmium on the cytoskeleton and morphology of the gill’s Chloride Cells (CCs) in the mosquito fish. In cadmium treated mosquito fish (0.03 mg/L for 28 days), the immuno-fluorescent light microscope showed strong absorption of actin stain and strong primary MT antibody uptake in CC and apical epithelia. Scanning electron microscopy observations showed a change in microfilament organized at the CC's apex, with the appearance of some actin filament aggregates. Higher cadmium concentrations (0.03 and 0.015 mg/L) did not alter such reorganization. Microtubules weren’t significantly affected by similar exposures. SEM analysis showed that cadmium exposure induced a significant increase in CC's surface area. After 28 days, the density of CC also increased. It was observed that there is an increase of CC's surface area, although the CC density didn’t increase significantly. The results of this study confirmed the cytotoxic effects of cadmium on mosquito fish.
Moh. Awaludin Adam; Maftuch Maftuch; Yuni Kilawati; Yenny Risjani. The effect of cadmium exposure on the cytoskeleton and morphology of the gill chloride cells in juvenile mosquito fish (Gambusia affinis). The Egyptian Journal of Aquatic Research 2019, 45, 337 -343.
AMA StyleMoh. Awaludin Adam, Maftuch Maftuch, Yuni Kilawati, Yenny Risjani. The effect of cadmium exposure on the cytoskeleton and morphology of the gill chloride cells in juvenile mosquito fish (Gambusia affinis). The Egyptian Journal of Aquatic Research. 2019; 45 (4):337-343.
Chicago/Turabian StyleMoh. Awaludin Adam; Maftuch Maftuch; Yuni Kilawati; Yenny Risjani. 2019. "The effect of cadmium exposure on the cytoskeleton and morphology of the gill chloride cells in juvenile mosquito fish (Gambusia affinis)." The Egyptian Journal of Aquatic Research 45, no. 4: 337-343.
Rosalina D, Herawati EY, Musa M, Sofarini D, Risjani Y. 2019. Short communication: Anatomical changes in the roots, rhizomes, and leaves of seagrass (Cymodocea serrulata) in response to lead. Biodiversitas 20: 2583-2588. Runoff of heavy metals as a result of urban and industrial development is a potential threat for seagrass populations in the coast. The objectives of this study were to study the anatomical changes in the tissues of roots, rhizomes, and leaves of seagrass Cymodocea serrulata in response to treatment with different concentrations of lead (Pb) for different time durations. This experiment used heavy metal Pb from a solution of Pb (NO3)2-with a concentration of 0 ppm, 5 ppm, 10 ppm, and 15 ppm and the treatment period extended up to 4 weeks with 3 replications. Analysis of changes in anatomical features showed that exodermis and endodermis cells in the roots thickened as lead concentration increased. The air spaces in the root cortex and rhizome also widened. Thickening of cell walls occurred in the epidermis and endodermis of rhizome. Likewise, in the leaves, thickening occurred in the upper and lower cuticle and also the upper and lower epidermis. In general, changes in anatomical features of root, rhizome, and leaves were observed in response to increasing lead concentrations. The results showed that C. serrulata developed some level of tolerance to heavy metals, especially lead.
Dwi Rosalina; Endang Yuli Herawati; Muhammad Musa; Dini Sofarini; Yenny Risjani. Short communication: Anatomical changes in the roots, rhizomes and leaves of seagrass (Cymodocea serrulata) in response to lead. Biodiversitas Journal of Biological Diversity 2019, 20, 1 .
AMA StyleDwi Rosalina, Endang Yuli Herawati, Muhammad Musa, Dini Sofarini, Yenny Risjani. Short communication: Anatomical changes in the roots, rhizomes and leaves of seagrass (Cymodocea serrulata) in response to lead. Biodiversitas Journal of Biological Diversity. 2019; 20 (9):1.
Chicago/Turabian StyleDwi Rosalina; Endang Yuli Herawati; Muhammad Musa; Dini Sofarini; Yenny Risjani. 2019. "Short communication: Anatomical changes in the roots, rhizomes and leaves of seagrass (Cymodocea serrulata) in response to lead." Biodiversitas Journal of Biological Diversity 20, no. 9: 1.
Background: As a marine organism, soft corals can be utilized to be various bioactive substances, especially terpenoids and steroids. The soft corals family which produces bioactive generally come from clavulariidae, alcyoniidae, nephtheidae and xeniidae family. Objective: To investigate the bioactivity of Nitric Oxide (NO) inhibitor release from soft coral crude extracts of Sinularia sp. (SCA), Nephthea sp. (SCB), Sarcophyton sp. (SCC), Sarcophyton sp. (SCD), Sinularia sp. (SCE) and Sinularia sp. (SCF). Materials and Methods: Soft coral is collected from Palu Bay (Central Sulawesi). NO inhibitory release activity measured according to the Griess reaction. Soft corals sample macerated with 1:2 (w/v). Then, Soft coral extracts with the best NO Inhibitor activity partitioned with Dichloromethane, Ethyl acetate, and n-butanol. The bioactive of all crude extracts were identified by GC-MS to find compounds with anti-inflammatory potential. Results: Sarcophyton sp. (SCC) and Sinularia sp. (SCF) are able to inhibit NO concentrations of 0.22 ± 0.04 and 0.20 ± 0.04 µM at 20 mg/mL, respectively. The chemical constituents determined and showed the potential as anti-inflammatory in the crude of Sinularia sp. (SCA) were Octacosane (3.25%). In Nephthea sp., (SCB) were Cyclohexene, 6-ethenyl-6- methyl-1-(1-methylethyl)-3-(1-methylethylidene)-,(S)- (0.55%); Azulene, 1,2,3,4,5,6,7,8- octahydro-1,4-dimethyl-7-(1-methylethylidene)-, (1S-cis)- (0.53%); and 1,7,7-Trimethyl- 2-vinylbicyclo[2.2.1]hept-2-ene (4.72%). In Sarcophyton sp, (SCC) were Eicosane (0.12%); Nonacosane (10.7%); 14(β)-Pregnane (0.87%); Octacosane 6.39%); and Tricosane (1.53%). In Sarcophyton sp. (SCD) were 14(β)-Pregnane (2.69%); and Octadecane (27.43%). In crude of Sinularia sp. (SCE) were Oleic Acid (0.63%); 7,10-Hexadecadienoic acid, methyl ester (0.54%); 14(β)-Pregnane (1.07%); 5,8,11,14-Eicosatetraenoic acid, ethyl ester, (all-Z)- (4.60%); Octacosane (7.75%); and 1,2-Benzisothiazole, 3-(hexahydro-1Hazepin- 1-yl)-, 1,1-dioxide (1.23%). In the crude of Sinularia sp., (SCF) were Oxirane, decyl- (1.38%); Nonacosane (0.57%); Cyclohexanol, 5-methyl-2-(1-methylethenyl)- (0.61%); 14B-Pregnane (0.76%); and Tetratriacontane (1.02%). Conclusion: The extract of Sarcophyton sp. (SCC) and Sinularia sp. (SCF) showed the best NO inhibitory release activity. This study is making soft corals from Central Sulawesi, Indonesia can become a potential organism in the discovery and development of bioactive substances anti-inflammatory.
Wendy Alexander Tanod; Uun Yanuhar; Maftuch; Masteria Yunovilsa Putra; Yenny Risjani. Screening of NO Inhibitor Release Activity from Soft Coral Extracts Origin Palu Bay, Central Sulawesi, Indonesia. Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry 2019, 18, 126 -141.
AMA StyleWendy Alexander Tanod, Uun Yanuhar, Maftuch, Masteria Yunovilsa Putra, Yenny Risjani. Screening of NO Inhibitor Release Activity from Soft Coral Extracts Origin Palu Bay, Central Sulawesi, Indonesia. Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry. 2019; 18 (2):126-141.
Chicago/Turabian StyleWendy Alexander Tanod; Uun Yanuhar; Maftuch; Masteria Yunovilsa Putra; Yenny Risjani. 2019. "Screening of NO Inhibitor Release Activity from Soft Coral Extracts Origin Palu Bay, Central Sulawesi, Indonesia." Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry 18, no. 2: 126-141.
Background and aims – The present study aims to describe a new species of pennate blue diatom from the genus Haslea, H. nusantara sp. nov., collected from Semak Daun Island, the Seribu Archipelago, in Indonesian marine waters. Methods – Assessment for species identification was conducted using light microscopy, Scanning Electron Microscopy and molecular techniques. The morphological characteristics of H. nusantara have been described, illustrated and compared to other morphologically similar blue Haslea taxa, distributed worldwide. Additionally, molecular characterization was achieved by sequencing plastidial and mitochondrial genomes. Key results – This new species, named Haslea nusantara, cannot be discriminated by its morphology (stria density) but it is characterized by its gene sequences (rbcL chloroplast gene and cox1 mitochondrial gene). Moreover, it differentiates from other blue Haslea species by the presence of a thin central bar, which has been previously reported in non-blue species like H. pseudostrearia. The complete mitochondrion (36,288 basepairs, bp) and plastid (120,448 bp) genomes of H. nusantara were sequenced and the gene arrangements were compared with other diatom genomes. Phylogeny analyses established using rbcL indicated that H. nusantara is included in the blue Haslea cluster and close to a blue Haslea sp. found in Canary Islands (H. silbo sp. ined.). Conclusions – All investigations carried out in this study show that H. nusantara is a new blue-pigmented species, which belongs to the blue Haslea clade, with an exceptional geographic distribution in the Southern Hemisphere.
Fiddy Semba Prasetiya; Romain Gastineau; Michel Poulin; Claude Lemieux; Monique Turmel; Agung Dhamar Syakti; Yann Hardivillier; Ita Widowati; Yenny Risjani; Iskandar Iskandar; Toto Subroto; Charlotte Falaise; Sulastri Arsad; Ikha Safitri; Jean-Luc Mouget; Vincent Leignel. Haslea nusantara (Bacillariophyceae), a new blue diatom from the Java Sea, Indonesia: morphology, biometry and molecular characterization. Plant Ecology and Evolution 2019, 152, 188 -202.
AMA StyleFiddy Semba Prasetiya, Romain Gastineau, Michel Poulin, Claude Lemieux, Monique Turmel, Agung Dhamar Syakti, Yann Hardivillier, Ita Widowati, Yenny Risjani, Iskandar Iskandar, Toto Subroto, Charlotte Falaise, Sulastri Arsad, Ikha Safitri, Jean-Luc Mouget, Vincent Leignel. Haslea nusantara (Bacillariophyceae), a new blue diatom from the Java Sea, Indonesia: morphology, biometry and molecular characterization. Plant Ecology and Evolution. 2019; 152 (2):188-202.
Chicago/Turabian StyleFiddy Semba Prasetiya; Romain Gastineau; Michel Poulin; Claude Lemieux; Monique Turmel; Agung Dhamar Syakti; Yann Hardivillier; Ita Widowati; Yenny Risjani; Iskandar Iskandar; Toto Subroto; Charlotte Falaise; Sulastri Arsad; Ikha Safitri; Jean-Luc Mouget; Vincent Leignel. 2019. "Haslea nusantara (Bacillariophyceae), a new blue diatom from the Java Sea, Indonesia: morphology, biometry and molecular characterization." Plant Ecology and Evolution 152, no. 2: 188-202.
The aim of this study was to assess the impact of cadmium nitrat (CdNO3) in Gambusia affinis on growth and the development of histopathological changes in gill organs and on the activity of some oxidative stress markers during a 96-hours acute toxicity test. Tests were performed on G. affinis. The average initial weight of fish used in the experiment was 3±1.2 g and the average initial total length of fish was 5.21±3.48 mm. The fish were exposed to a range of CdNO3 concentrations (1 mg/L, environmental concentration, 0.1, 0.01, 0.001 nad 0.0001 mg/L) for 96 hours. Each test on a CdNO3-treated group was performed in duplicate. There were no significant differences between the specific growth rates of fish from the test groups and from the control group. Histopathological examination revealed no pathological changes in organs of fish exposed to any CdNO3 concentration. Significantly lower CAT and SOD activity (p0.05). No significance differences (p>0.05) between the control group and any experimental group were found with respect to Protease activity. The MDA level was significantly higher (p0.05) were observed. According to our results, all tested concentrations of CdNO3, including the environmental concentration, had an inhibit on oxidative stress markers and detoxifying enzymes in exposed fish, but did not affect fish growth or cause the development of histopathological changes in the fish organism.
M A Adam; Maftuch; Y Kilawati; A Soegianto; Y Risjani. The effects of acute exposure to cadmium nitrate (CdNO3) on gambusia fish (Gambusia affinis). IOP Conference Series: Earth and Environmental Science 2019, 259, 012004 .
AMA StyleM A Adam, Maftuch, Y Kilawati, A Soegianto, Y Risjani. The effects of acute exposure to cadmium nitrate (CdNO3) on gambusia fish (Gambusia affinis). IOP Conference Series: Earth and Environmental Science. 2019; 259 (1):012004.
Chicago/Turabian StyleM A Adam; Maftuch; Y Kilawati; A Soegianto; Y Risjani. 2019. "The effects of acute exposure to cadmium nitrate (CdNO3) on gambusia fish (Gambusia affinis)." IOP Conference Series: Earth and Environmental Science 259, no. 1: 012004.
Dwi Rosalina Dwi Rosalina; Endang Yuli Herawati; Muhammad Musa; Dini Sofarini; Mohammad Amin; Yenny Risjani. Lead Accumulation and Its Histological Impact on Cymodocea serrulata Seagrass in the Laboratory. Sains Malaysiana 2019, 48, 813 -822.
AMA StyleDwi Rosalina Dwi Rosalina, Endang Yuli Herawati, Muhammad Musa, Dini Sofarini, Mohammad Amin, Yenny Risjani. Lead Accumulation and Its Histological Impact on Cymodocea serrulata Seagrass in the Laboratory. Sains Malaysiana. 2019; 48 (4):813-822.
Chicago/Turabian StyleDwi Rosalina Dwi Rosalina; Endang Yuli Herawati; Muhammad Musa; Dini Sofarini; Mohammad Amin; Yenny Risjani. 2019. "Lead Accumulation and Its Histological Impact on Cymodocea serrulata Seagrass in the Laboratory." Sains Malaysiana 48, no. 4: 813-822.
Antioxidants is a substance widely explored and sought after, due to the increase of free radicals in the human body, is very closely linked to the human degenerative disease. Soft corals are also reported to produce compounds that show antioxidant activity. This research aimed to examine the antioxidant and profile bioactive of six soft corals species from three genera Sinularia, Sarcophyton and Nephthea origin Palu Bay, Central Sulawesi. The antioxidant activity was measured by the DPPH radical scavenging method. Profile bioactive were identified using GC-MS spectra analysis. The results of IC50 analysis showed the crudes of soft corals belonging to the very strong antioxidant activity. Sinularia sp. (SCA) has the highest (IC50 = 10.708 ± 0.374 μg/mL). The GC-MS analysis showed 2-Dodecen-1-yl (-) succinic anhydrid; Hexadecanoic acid, methyl ester; Bacchotricuneatin c; L - (+) - Ascorbic acid 2,6-dihexadecanoate; 14 (β) -Pregnane; Octacosane; Heptacosane; and Tetrapentacontane, 1,54-dibromoare common compounds that have the potential as antioxidants. This study provides on the potential soft corals origin Palu Bay, Central Sulawesi as a source of antioxidant. This study also shows the compounds in soft coral according to GC-MS.This is a new report of antioxidant substances from Nephthea sp. origin of Indonesian oceans.
W A Tanod; U Yanuhar; Maftuch; D Wahyudi; Y Risjani. DPPH scavenging property of bioactives from soft corals origin palu bay, Central Sulawesi, Indonesia. IOP Conference Series: Earth and Environmental Science 2019, 236, 012121 .
AMA StyleW A Tanod, U Yanuhar, Maftuch, D Wahyudi, Y Risjani. DPPH scavenging property of bioactives from soft corals origin palu bay, Central Sulawesi, Indonesia. IOP Conference Series: Earth and Environmental Science. 2019; 236 (1):012121.
Chicago/Turabian StyleW A Tanod; U Yanuhar; Maftuch; D Wahyudi; Y Risjani. 2019. "DPPH scavenging property of bioactives from soft corals origin palu bay, Central Sulawesi, Indonesia." IOP Conference Series: Earth and Environmental Science 236, no. 1: 012121.
Hasleaostrearia is a marine diatom that can feed oysters. This study aimed to cultivate H.ostrearia in the following media: seawater (SW), seawater filtered by activated carbon (SW+CA), and drilling water (DW). The three-water media were supplemented with two types of nutrients, Walne-Conway+silica (CS) and ES-1/3 Provasoli (P), leading to a six experiment series. The cultures were grown in 200 mL and 400 mL respectively. The dilution cycle was every 3 - 4 day for 200 mL volume and every 4 - 5 days for the 400 mL volume for 20 - 40 days or until the cultures showed a decrease in growth. The microalgae exhibited a better growth in SW and SW+CA CS, whereas DW showed a diminishing number of cells in both the CS and P medium. The highest value of Emn in the 200 mL volume presented in the SW CS medium (1.94 mg L−1) and in the SW+CA CS medium (8.16 mg L−1), while the lowest value of Emn was found in the SW+CA CS medium (0.42 mg L−1). Imn was apparent in the SW P medium (0.56 mg L−1). In conclusion, both SW CS and SW+CA CS showed a better performance in growth. The seawater was more stable during the experiment in both the 200 mL and 400 mL volumes.
S Arsad; C Stavrakakis; V Turpin; P Rossa; Y Risjani; Luthfiana Aprilianita Sari; F S Prasetiya; J-L Mouget. Optimization of diatom Hasleao strearia cultivation in different mediums and nutrients. IOP Conference Series: Earth and Environmental Science 2019, 236, 012044 .
AMA StyleS Arsad, C Stavrakakis, V Turpin, P Rossa, Y Risjani, Luthfiana Aprilianita Sari, F S Prasetiya, J-L Mouget. Optimization of diatom Hasleao strearia cultivation in different mediums and nutrients. IOP Conference Series: Earth and Environmental Science. 2019; 236 (1):012044.
Chicago/Turabian StyleS Arsad; C Stavrakakis; V Turpin; P Rossa; Y Risjani; Luthfiana Aprilianita Sari; F S Prasetiya; J-L Mouget. 2019. "Optimization of diatom Hasleao strearia cultivation in different mediums and nutrients." IOP Conference Series: Earth and Environmental Science 236, no. 1: 012044.
Title (Bahasa Indonesia): Histopatologi daging dan hati ikan kakap putih (Lates calcarifer) pada musim berbeda dari Perairan Teluk Kao yang tercemar merkuri (Hg), Halmahera Utara, Indonesia. Fish caught in Kao Gulf waters has been contaminated by mercury in low concentration. This study aimed to identify and analyze the impact of mercury (Hg). Water, sediment and fish samples were taken using a plastic scoop, a grab, and fishing. Hg content analysis used AAS (atomic absorption spectrophotometer). While the histopathological analysis of the fish meat and liver used automated slide stainer and microscope. Hg concentration of Kobok and Taolas river water in dry season and rainy season was not safe to consume according to Indonesian National Standard of 7387/2009 for natural mineral water. Hg concentration of the sediment also did not meet the Indonesian quality standard according to Government Regulation Numbered 82/2001. Fish were still allowed to be consumed. The meat and liver histopathology in dry and rainy season had tissue cell change, such as edema, degeneration hydrophic, and lamellae fusion. Ikan yang tertangkap di Perairan Teluk Kao telah terkontaminasi dengan merkuri dalam jumlah rendah. Penelitian ini bertujuan untuk mengetahui dan menganalisis dampak pencemaran merkuri (Hg) dengan menginformasikan keamanan konsumsi ikan Kakap Putih di perairan Teluk Kao Halmahera Utara. Sampel air diambil mengunakan gayung plastik, sampel sedimen dengan alat grab sampler sedangkan sampel ikan mengunakan alat pacing. Analisis kandungan Hg air, sedimen dan ikan dengan metode AAS (atomic absorption spectrophotometer). Analisis histopatologi daging dan hati ikan dengan alat automated slide stainer dan mikroskop. Hasil menunjukkan bahwa Nilai Hg air sungai Kobok dan Taolas pada musim panas dan musim hujan tidak layak dikonsumsi menurut SNI 7387/2009. Konsentrasi Hg dalam sedimen juga tidak memenuhi Standar Baku Mutu Indonesia menurut Peraturan Pemerintah No. 82 Tahun 2001. Ikan kakap putih masih diizinkan untuk dikonsumsi. Histopatologi daging dan hati ikan kakap putih pada musim panas dan musim hujan yang mengalami perubahan jaringan sel, seperti mengalami edema, degenerasi hidropis, dan fusi lamellae.
Azis Husen; Endang Yuli Herawati; Yenny Risjani. Seasonal variation in meat and liver histopathology of white snapper (Lates calcarifer) from mercury-polluted Kao Gulf Waters, North Halmahera, Indonesia. AQUATIC SCIENCE & MANAGEMENT 2016, 4, 1 -10.
AMA StyleAzis Husen, Endang Yuli Herawati, Yenny Risjani. Seasonal variation in meat and liver histopathology of white snapper (Lates calcarifer) from mercury-polluted Kao Gulf Waters, North Halmahera, Indonesia. AQUATIC SCIENCE & MANAGEMENT. 2016; 4 (1):1-10.
Chicago/Turabian StyleAzis Husen; Endang Yuli Herawati; Yenny Risjani. 2016. "Seasonal variation in meat and liver histopathology of white snapper (Lates calcarifer) from mercury-polluted Kao Gulf Waters, North Halmahera, Indonesia." AQUATIC SCIENCE & MANAGEMENT 4, no. 1: 1-10.
The traditional gold mining in Cempaka, Banjarbaru, South Kalimantan, Indonesia used mercury in order to separate the gold with a fine sand and rocks. Mercury can impair the function of the cellular and organism physiology that live in the mining sites. In cellular on earthworm may adapt to help in repairing cell damage with expression of HSP70 proteins. This study was conducted in November 2012 to September 2014. Sampling of soil and earthworms do in Banjarbaru village (control) and in paddy field near gold mining among them Murung village, Pumpung village and Palam village (ex. PT. Galuh Cempaka). Soil quality in the gold mining indicate conditions are still good in term of C-organic, pH and CEC. The C-organic content of the soil between the 2.08-2.52%; the value of pH H2O between 4.90-5.72, CEC between 17.20-18.11. Detection of protein HSP70 on earthworms use the method immunohistochemistry. HSP70 expression was found in the longitudinal muscle layer (LM). The expression of HSP70 most occurred in earthworms that live in the land of the paddy fields ex. PT. Galuh Cempaka.
Setya Widi Ayuning; Diana Arfiati; Yenny Risjani. Immunohistochemistry of Heat Shock Protein 70 (Hsp70) on Earthworm (Lumbricus terrestris) in Gold Mining Sites. Journal of Applied Biotechnology 2014, 2, 102 .
AMA StyleSetya Widi Ayuning, Diana Arfiati, Yenny Risjani. Immunohistochemistry of Heat Shock Protein 70 (Hsp70) on Earthworm (Lumbricus terrestris) in Gold Mining Sites. Journal of Applied Biotechnology. 2014; 2 (2):102.
Chicago/Turabian StyleSetya Widi Ayuning; Diana Arfiati; Yenny Risjani. 2014. "Immunohistochemistry of Heat Shock Protein 70 (Hsp70) on Earthworm (Lumbricus terrestris) in Gold Mining Sites." Journal of Applied Biotechnology 2, no. 2: 102.
Since May 29, 2006, a mud volcano in the Brantas Delta of the Sidoarjo district has emitted mud that has inundated nearby villages. Pollution in this area has been implicated in detrimental effects on fish health. In fishes, leukocyte and phagocytic cells play a vital role in body defenses. We report for the first time the effect of "LUSI" volcano mud on the immune systems of fish in the Brantas Delta. The aim of this study was to find biomarkers to allow the evaluation of the effects of volcanic mud and anthropogenic pollution on fish health in the Brantas Delta. The study took places at the Brantas Delta, which was polluted by volcano mud, and at reference sites in Karangkates and Pasuruan. Leukocyte numbers were determined using a Neubauer hemocytometer and a light microscope. Differential leukocyte counts were determined using blood smears stained with May Grunwald-Giemsa, providing neutrophil, lymphocyte and monocyte counts. Macrophages were taken from fish kidney, and their phagocytic activity was measured. In vitro analyses revealed that leukocyte and differential leukocyte counts (DLC) were higher in Channa striata and Chanos chanos caught from the polluted area. Macrophage numbers were higher in Oreochromis mossambicus than in the other species, indicating that this species is more sensitive to pollution. In areas close to volcanic mud eruption, all specimens had lower phagocytic activity. Our results show that immune cells were changed and phagocytic activity was reduced in the polluted area indicating cytotoxicity and alteration of the innate immune system in fishes exposed to LUSI volcano mud and anthropogenic pollution.
Yenny Risjani; Yunianta Yunianta; Jerome Couteau; Christophe Minier. Cellular immune responses and phagocytic activity of fishes exposed to pollution of volcano mud. Marine Environmental Research 2014, 96, 73 -80.
AMA StyleYenny Risjani, Yunianta Yunianta, Jerome Couteau, Christophe Minier. Cellular immune responses and phagocytic activity of fishes exposed to pollution of volcano mud. Marine Environmental Research. 2014; 96 ():73-80.
Chicago/Turabian StyleYenny Risjani; Yunianta Yunianta; Jerome Couteau; Christophe Minier. 2014. "Cellular immune responses and phagocytic activity of fishes exposed to pollution of volcano mud." Marine Environmental Research 96, no. : 73-80.