Faced with the scarcity of water resource and irrational fertilizer use, it is highly important to supply plants with water and fertilizer at desiderated stages to improve yield with high water use efficiency (WUE). A pot experiment was conducted to investigate the effects of growth stage-specific water deficiency and potassium (K) fertilization on tomato yield and WUE. The entire growing season of tomato was divided into 5 stages: vegetative growth stage (VG), flowering and fruit setting stage (FS), early fruit growth stage (FG), fruit development stage (FD) and fruit maturity stage (FM). Three soil moisture (W) and three K fertilization levels were set up. W levels included W1, W2 and W3, indicating that soil water was maintained at 60–70% field capacity, 70–80% field capacity, and 80–90% field capacity, respectively. K levels included K1, K2 and K3, indicating that 0 g K₂O per kg soil, 0.46 g K₂O per kg soil and 0.92 g K₂O per kg soil was applied. All combinations of the three W and three K levels were solely imposed at each of the five growth stages, for other four stages, plants were watered to 80–90% field capacity without K fertilizer (W3K1). The permanent W3K1 over the entire growth stage was taken as control (CK). The results showed that W deficiency imposed at all stages significantly affected tomato yield (P<0.01), except for VG stage in which W deficiency did not cause yield loss. K fertilization level during FS or FM stage had a significant effect on yield (P<0.01). A significant interaction effect of W and K on yield was only observed during FM stage. For WUE, significant effect of W deficiency at FS, FD and FM stages were observed, and a significant effect of K levels at FS, FD and FM stages was observed. Specifically, K fertilization was necessary during specific growth stage of tomato (i.e. FS and FM). During FS stage, even if a sufficient water supply seems necessary, a deficit irrigation with K fertilization could be applied as K fertilization could alleviate the negative effect of soil water deficit, however, excess of K fertilization during FM stage should be avoided to maintain tomato yield and WUE.

The objective of this study was to explore the effects of plant growth-promoting rhizobacteria (PGPR), strain Bacillus licheniformis, with softwood biochar amendment on potato growth and water use efficiency (WUE) under a deficit irrigation (DI) regime. A pot experiment was conducted in a greenhouse. The results showed that PGPR improved leaf gas exchange rates, including photosynthesis rate, stomatal conductance and transpiration rate at early seedling stage, while tended to depress these parameters gradually until final harvest. The effects of biochar on plant leaf physiology, plant growth and WUE were not evident. Plants were more affected by DI than PGPR inoculation and biochar amendment. DI significantly decreased leaf gas exchange rates after exposure to water treatment for around three weeks, and the negative effect was eliminated at final harvest. At final harvest, DI significantly decreased leaf area, specific leaf area, dry mass of leaf and stem, total dry mass, dry mass increment and plant water use. The synergistical effect of PGPR strain Bacillus licheniformis and DI on plant growth and WUE were not observed in our study. WUE was solely improved by DI, indicating that, compared to PGPR inoculation, DI was a more effective measure to enhance plant WUE.

A field experiment was carried out to investigate effect of nitrogen (N) supply method on root growth and its correlation with the above-ground parts in maize (Zea mays L.) under alternate partial root-zone irrigation (APRI) at Wuwei, northwest China in 2012 and 2014. The treatments included alternate N supply, conventional N supply and fixed N supply under APRI (designated AN, CN and FN, respectively), with an additional CN fertilizer treatment coupled with conventional irrigation (CK). Ridges were built in a west-east direction. Root weight density (RWD) in the 0–100 cm soil layer and shoot biomass at the V₆, V₁₂, VT, R₂ and R₆ stages, and grain yield and yield components at the R₆ were determined. Results showed that RWD around the plant (i.e. under the plant, south and north of the plant) in the 0–40 cm soil layer varied among different treatments at the VT, R₂ and R₆ stages. The RWD north and south the plant were comparable during maize growth stages for AN, CN and CK, while FN significantly decreased the RWD of its no N supply side at the three stages and markedly decreased the RWD of its N supply side at the VT. AN and CN significantly increased the RWD, shoot biomass at the three stages, and grain yield compared with FN and CK. Grain yield was positively correlated with RWD in the 0–40 cm soil layer at the three stages. These results suggested that AN and CN produced a relatively uniform distribution of roots and a greater root biomass, which contributed to the enhanced shoot biomass and grain yield of maize under APRI.

Root growth and its adaptation are quite important for efficient use of water and nutrients. The present study investigated the effects of alternate partial root-zone irrigation (APRI) coupled with nitrogen application on root growth and nitrogen-use efficiency (NUE) of maize (Zea mays L.). Treatments (conducted at Wuwei City, northwest China) included APRI with controlled irrigation at 45%, 65%, and 80% of field capacity (FC) (W1, W2, and W3, respectively) coupled with nitrogen application rate of 100, 200, and 300 kg N ha⁻¹ (N1, N2, and N3, respectively). The study found a significant interaction effect of irrigation regimes and nitrogen application rates on maize root growth and NUE. Compared with N2, N3 did not increase the length, surface area, weight, and volume of roots at the filling and maturity stages under W2, while it did under W3, which suggests that increasing nitrogen application rates did not compensate for the adverse effect of drought on root growth. In addition, NUE positively correlated with these root morphological parameters but negatively with the root-to-shoot ratio at the filling and maturity stages. The W2N2 treatment with moderate soil moisture and relatively high soil nitrate-nitrogen promoted total root growth and deeper roots and optimized vertical root distribution, with a low root-to-shoot ratio, resulting in the greatest NUE. These results suggest that moderately controlled irrigation (W2) combined with a reasonable nitrogen rate (N2) improves root growth and optimizes root distribution, resulting in a high NUE in maize under alternate partial root-zone irrigation.