This page has only limited features, please log in for full access.

Dr. Huijun Wu
Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P. R. China

Basic Info

Basic Info is private.

Research Keywords & Expertise

0 volatile organic compounds
0 Induced systemic resistance
0 Biocontrol agents
0 Antimicrobial mechanisms of active substances
0 Biocontrol of plant diseases

Fingerprints

Plant growth-promoting and biocontrol microorganisms
Induced systemic resistance
Biocontrol of plant diseases

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 18 February 2021 in Pathogens
Reads 0
Downloads 0

Biological nematicides have been widely used to lower the losses generated by phytoparasitic nematodes. The purpose of this study was to evaluate the nematicidal effects of Escherichia coli BL21(DE3) against Meloidogyne javanica and to identify nematicide-related genes. Culture filtrates of BL21(DE3) caused juvenile mortality and inhibited egg hatching in a dose-dependent manner. In the greenhouse, treatment of tomato seedlings with BL21(DE3) culture filtrates at 50 and 100% concentrations not only reduced the amount of M. javanica egg masses and galls, but improved plant root and shoot fresh weight. Culture filtrate analysis indicated that the nematicidal active ingredients of strain BL21(DE3) were non-proteinaceous, heat and cold resistant, sensitive to pH and volatile. To identify the genes associated with nematicidal activity, a BL21(DE3) library of 5000 mutants was produced using Tn5 transposase insertion. The culture filtrate of the MB12 mutant showed no nematicidal activity after 72 h of treatment and thermal asymmetrical interlaced PCR demonstrated that the carB gene was disrupted. Nematicidal activity was restored when the pH of the MB12 culture filtrate was adjusted to the original pH value (4.15) or following MB12 complementation with the carB gene, confirming a role for carB in mediating pH value and nematicidal activity. The outcomes of this pilot study indicate that BL21(DE3) is a potential microorganism for the continuable biological control of root-knot nematode in tomato and that carB affects the nematicidal activity of BL21(DE3) by modulating the pH environment.

ACS Style

Yanfei Xia; Shen Li; Guohui Xu; Shanshan Xie; Xueting Liu; Xiaomin Lin; Huijun Wu; Xuewen Gao. The carB Gene of Escherichia coli BL21(DE3) is Associated with Nematicidal Activity against the Root-Knot Nematode Meloidogyne javanica. Pathogens 2021, 10, 222 .

AMA Style

Yanfei Xia, Shen Li, Guohui Xu, Shanshan Xie, Xueting Liu, Xiaomin Lin, Huijun Wu, Xuewen Gao. The carB Gene of Escherichia coli BL21(DE3) is Associated with Nematicidal Activity against the Root-Knot Nematode Meloidogyne javanica. Pathogens. 2021; 10 (2):222.

Chicago/Turabian Style

Yanfei Xia; Shen Li; Guohui Xu; Shanshan Xie; Xueting Liu; Xiaomin Lin; Huijun Wu; Xuewen Gao. 2021. "The carB Gene of Escherichia coli BL21(DE3) is Associated with Nematicidal Activity against the Root-Knot Nematode Meloidogyne javanica." Pathogens 10, no. 2: 222.

Biotechnologically relevant enzymes and proteins
Published: 12 October 2020 in Applied Microbiology and Biotechnology
Reads 0
Downloads 0

Several quorum sensing systems occurring in Bacillus subtilis, e.g. Rap-Phr systems, were reported to interact with major regulatory proteins, such as ComA, DegU, and Spo0A, in order to regulate competence, sporulation, and synthesis of secondary metabolites. In this study, we characterized a novel Rap-Phr system, RapA4-PhrA4, in Bacillus velezensis NAU-B3. We found that the rapA4 and phrA4 genes were co-transcribed in NAU-B3. When rapA4 was expressed in the heterologous host Bacillus subtilis OKB105, surfactin production and sporulation were severely inhibited. However, when the phrA4 was co-expressed, the RapA4 activity was inhibited. The transcription of the surfactin synthetase srfA gene and sporulation-related genes were also regulated by the RapA4-PhrA4 system. In vitro results obtained from electrophoretic mobility shift assay (EMSA) proved that RapA4 inhibits ComA binding to the promoter of the srfA operon, and the PhrA4 pentapeptide acts as anti-activator of RapA4. We also found that the F24 residue plays a key role in RapA4 function. This study indicated that the novel RapA4-PhrA4 system regulates the surfactin synthesis and sporulation via interaction with ComA, thereby supporting the bacterium to compete and to survive in a hostile environment. •Bacillus velezensis NAU-B3 has a novel Rap-Phr quorum sensing system, which does not occur in model strains Bacillus subtilis 168 and B. velezensis FZB42. •RapA4-PhrA4 regulates surfactin production and sporulation. •RapA4-PhrA4 interacts with the ComA protein from ComP/ComA two-component system.

ACS Style

Zhao Liang; Jun-Qing Qiao; Ping-Ping Li; Lu-Lu Zhang; Zi-Xuan Qiao; Ling Lin; Chen-Jie Yu; Yang Yang; Muhammad Zubair; Qin Gu; Hui-Jun Wu; Rainer Borriss; Xue-Wen Gao. A novel Rap-Phr system in Bacillus velezensis NAU-B3 regulates surfactin production and sporulation via interaction with ComA. Applied Microbiology and Biotechnology 2020, 104, 10059 -10074.

AMA Style

Zhao Liang, Jun-Qing Qiao, Ping-Ping Li, Lu-Lu Zhang, Zi-Xuan Qiao, Ling Lin, Chen-Jie Yu, Yang Yang, Muhammad Zubair, Qin Gu, Hui-Jun Wu, Rainer Borriss, Xue-Wen Gao. A novel Rap-Phr system in Bacillus velezensis NAU-B3 regulates surfactin production and sporulation via interaction with ComA. Applied Microbiology and Biotechnology. 2020; 104 (23):10059-10074.

Chicago/Turabian Style

Zhao Liang; Jun-Qing Qiao; Ping-Ping Li; Lu-Lu Zhang; Zi-Xuan Qiao; Ling Lin; Chen-Jie Yu; Yang Yang; Muhammad Zubair; Qin Gu; Hui-Jun Wu; Rainer Borriss; Xue-Wen Gao. 2020. "A novel Rap-Phr system in Bacillus velezensis NAU-B3 regulates surfactin production and sporulation via interaction with ComA." Applied Microbiology and Biotechnology 104, no. 23: 10059-10074.

Journal article
Published: 27 April 2020 in Postharvest Biology and Technology
Reads 0
Downloads 0

Biological control utilizing antagonistic yeasts has been actively pursued as an alternative to synthetic fungicides for the management of postharvest diseases. Abiotic stress resistance is an important attribute for antagonistic yeasts, directly associated with their biocontrol efficacy. The MADS-box transcription factor, Rlm1, has been reported to regulate the response of model yeasts to cell wall stress. Rlm1 in the antagonistic yeast, Candida oleophila, was found to play a role in resistance to salt, heat, and oxidative stress. Two Rlm1 mutants (ΔRlm1-1 and ΔRlm1-2) were generated. Compared to the wild-type (WT), C. oleophila I-182, ΔRlm1-1, and ΔRlm1-2 were more sensitive to a variety of stresses, including heat, salt, and oxidative stress. The mutants also exhibited lower biocontrol efficacy against gray mold caused by Botrytis cinerea, and slower growth in kiwifruit wounds with respect to the WT. This study provided the information to understand the relationship between the Rlm1 transcription factor, stress resistance, and biocontrol efficacy of antagonistic yeasts used for the biocontrol of postharvest diseases.

ACS Style

Yuan Sui; Zhiqiang Sun; Yuping Zou; Wenhua Li; Mingguo Jiang; Yuzhen Luo; Wenjian Liao; Yuanhong Wang; Xuewen Gao; Jia Liu; Huijun Wu. The Rlm1 transcription factor in Candida oleophila contributes to abiotic stress resistance and biocontrol efficacy against postharvest gray mold of kiwifruit. Postharvest Biology and Technology 2020, 166, 111222 .

AMA Style

Yuan Sui, Zhiqiang Sun, Yuping Zou, Wenhua Li, Mingguo Jiang, Yuzhen Luo, Wenjian Liao, Yuanhong Wang, Xuewen Gao, Jia Liu, Huijun Wu. The Rlm1 transcription factor in Candida oleophila contributes to abiotic stress resistance and biocontrol efficacy against postharvest gray mold of kiwifruit. Postharvest Biology and Technology. 2020; 166 ():111222.

Chicago/Turabian Style

Yuan Sui; Zhiqiang Sun; Yuping Zou; Wenhua Li; Mingguo Jiang; Yuzhen Luo; Wenjian Liao; Yuanhong Wang; Xuewen Gao; Jia Liu; Huijun Wu. 2020. "The Rlm1 transcription factor in Candida oleophila contributes to abiotic stress resistance and biocontrol efficacy against postharvest gray mold of kiwifruit." Postharvest Biology and Technology 166, no. : 111222.

Journal article
Published: 24 May 2019 in Toxins
Reads 0
Downloads 0

Fusarium graminearum is a notorious pathogen that causes Fusarium head blight (FHB) in cereal crops. It produces secondary metabolites, such as deoxynivalenol, diminishing grain quality and leading to lesser crop yield. Many strategies have been developed to combat this pathogenic fungus; however, considering the lack of resistant cultivars and likelihood of environmental hazards upon using chemical pesticides, efforts have shifted toward the biocontrol of plant diseases, which is a sustainable and eco-friendly approach. Fengycin, derived from Bacillus amyloliquefaciens FZB42, was purified from the crude extract by HPLC and further analyzed by MALDI-TOF-MS. Its application resulted in structural deformations in fungal hyphae, as observed via scanning electron microscopy. In planta experiment revealed the ability of fengycin to suppress F. graminearum growth and highlighted its capacity to combat disease incidence. Fengycin significantly suppressed F. graminearum, and also reduced the deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), and zearalenone (ZEN) production in infected grains. To conclude, we report that fengycin produced by B. amyloliquefaciens FZB42 has potential as a biocontrol agent against F. graminearum and can also inhibit the mycotoxins produced by this fungus.

ACS Style

Alvina Hanif; Feng Zhang; Pingping Li; Chuchu Li; Yujiao Xu; Muhammad Zubair; Mengxuan Zhang; Dandan Jia; Xiaozhen Zhao; Jingang Liang; Taha Majid; Jingyuau Yan; Ayaz Farzand; Huijun Wu; Qin Gu; Xuewen Gao. Fengycin Produced by Bacillus amyloliquefaciens FZB42 Inhibits Fusarium graminearum Growth and Mycotoxins Biosynthesis. Toxins 2019, 11, 295 .

AMA Style

Alvina Hanif, Feng Zhang, Pingping Li, Chuchu Li, Yujiao Xu, Muhammad Zubair, Mengxuan Zhang, Dandan Jia, Xiaozhen Zhao, Jingang Liang, Taha Majid, Jingyuau Yan, Ayaz Farzand, Huijun Wu, Qin Gu, Xuewen Gao. Fengycin Produced by Bacillus amyloliquefaciens FZB42 Inhibits Fusarium graminearum Growth and Mycotoxins Biosynthesis. Toxins. 2019; 11 (5):295.

Chicago/Turabian Style

Alvina Hanif; Feng Zhang; Pingping Li; Chuchu Li; Yujiao Xu; Muhammad Zubair; Mengxuan Zhang; Dandan Jia; Xiaozhen Zhao; Jingang Liang; Taha Majid; Jingyuau Yan; Ayaz Farzand; Huijun Wu; Qin Gu; Xuewen Gao. 2019. "Fengycin Produced by Bacillus amyloliquefaciens FZB42 Inhibits Fusarium graminearum Growth and Mycotoxins Biosynthesis." Toxins 11, no. 5: 295.

Journal article
Published: 01 October 2017 in Applied and Environmental Microbiology
Reads 0
Downloads 0

Fusarium graminearum (teleomorph: Ascomycota, Hypocreales, Gibberella , Gibberella zeae ) is a destructive fungal pathogen that threatens the production and quality of wheat and barley worldwide. Controlling this toxin-producing pathogen is a significant challenge. In the present study, the commercially available strain Bacillus amyloliquefaciens ( Bacteria , Firmicutes , Bacillales , Bacillus ) FZB42 showed strong activity against F. graminearum . The lipopeptide bacillomycin D, produced by FZB42, was shown to contribute to the antifungal activity. Purified bacillomycin D showed strong activity against F. graminearum , and its 50% effective concentration was determined to be approximately 30 μg/ml. Analyses using scanning and transmission electron microscopy revealed that bacillomycin D caused morphological changes in the plasma membranes and cell walls of F. graminearum hyphae and conidia. Fluorescence microscopy combined with different dyes showed that bacillomycin D induced the accumulation of reactive oxygen species and caused cell death in F. graminearum hyphae and conidia. F. graminearum secondary metabolism also responded to bacillomycin D challenge, by increasing the production of deoxynivalenol. Biological control experiments demonstrated that bacillomycin D exerted good control of F. graminearum on corn silks, wheat seedlings, and wheat heads. In response to bacillomycin D, F. graminearum genes involved in scavenging reactive oxygen species were downregulated, whereas genes involved in the synthesis of deoxynivalenol were upregulated. Phosphorylation of MGV1 and HOG1, the mitogen-activated protein kinases of F. graminearum , was increased in response to bacillomycin D. Taken together, these findings reveal the mechanism of the antifungal action of bacillomycin D. IMPORTANCE Biological control of plant disease caused by Fusarium graminearum is desirable. Bacillus amyloliquefaciens FZB42 is a representative of the biocontrol bacterial strains. In this work, the lipopeptide bacillomycin D, produced by FZB42, showed strong fungicidal activity against F. graminearum . Bacillomycin D caused morphological changes in the plasma membrane and cell wall of F. graminearum , induced accumulation of reactive oxygen species, and ultimately caused cell death in F. graminearum . Interestingly, when F. graminearum was challenged with bacillomycin D, the deoxynivalenol production, gene expression, mitogen-activated protein kinase phosphorylation, and pathogenicity of F. graminearum were significantly altered. These findings clarified the mechanisms of the activity of bacillomycin D against F. graminearum and highlighted the potential of B. amyloliquefaciens FZB42 as a biocontrol agent against F. graminearum .

ACS Style

Qin Gu; Yang Yang; Qiming Yuan; Guangming Shi; Liming Wu; Zhiying Lou; Rong Huo; Huijun Wu; Rainer Borriss; Xuewen Gao. Bacillomycin D Produced by Bacillus amyloliquefaciens Is Involved in the Antagonistic Interaction with the Plant-Pathogenic Fungus Fusarium graminearum. Applied and Environmental Microbiology 2017, 83, e01075-17 .

AMA Style

Qin Gu, Yang Yang, Qiming Yuan, Guangming Shi, Liming Wu, Zhiying Lou, Rong Huo, Huijun Wu, Rainer Borriss, Xuewen Gao. Bacillomycin D Produced by Bacillus amyloliquefaciens Is Involved in the Antagonistic Interaction with the Plant-Pathogenic Fungus Fusarium graminearum. Applied and Environmental Microbiology. 2017; 83 (19):e01075-17.

Chicago/Turabian Style

Qin Gu; Yang Yang; Qiming Yuan; Guangming Shi; Liming Wu; Zhiying Lou; Rong Huo; Huijun Wu; Rainer Borriss; Xuewen Gao. 2017. "Bacillomycin D Produced by Bacillus amyloliquefaciens Is Involved in the Antagonistic Interaction with the Plant-Pathogenic Fungus Fusarium graminearum." Applied and Environmental Microbiology 83, no. 19: e01075-17.

Journal article
Published: 01 October 2017 in Fungal Genetics and Biology
Reads 0
Downloads 0

Histone H3 lysine 36 methylation (H3K36me) is generally associated with activation of gene expression in most eukaryotic cells. However, the function of H3K36me in filamentous fungi is largely unknown. Set2 is the sole lysine histone methyltransferase (KHMTase) enzyme responsible for the methylation of H3K36 in Saccharomyces cerevisiae. In the current study, we identified a single ortholog of S. cerevisiae Set2 in Fusarium verticillioides. We report that FvSet2 is responsible for the trimethylation of H3K36 (H3K36me3). The FvSET2 deletion mutant (ΔFvSet2) showed significant defects in vegetative growth, FB1 biosynthesis, pigmentation, and fungal virulence. Furthermore, trimethylation of H3K36 was found to be important for active transcription of genes involved in FB1 and bikaverin biosyntheses. These data indicate that FvSet2 plays an important role in the regulation of secondary metabolism, vegetative growth and fungal virulence in F. verticillioides.

ACS Style

Qin Gu; Zhenzhong Wang; Xiao Sun; Tiantian Ji; Hai Huang; Yang Yang; Hao Zhang; Hafiz Abdul Samad Tahir; Liming Wu; Huijun Wu; Xuewen Gao. FvSet2 regulates fungal growth, pathogenicity, and secondary metabolism in Fusarium verticillioides. Fungal Genetics and Biology 2017, 107, 24 -30.

AMA Style

Qin Gu, Zhenzhong Wang, Xiao Sun, Tiantian Ji, Hai Huang, Yang Yang, Hao Zhang, Hafiz Abdul Samad Tahir, Liming Wu, Huijun Wu, Xuewen Gao. FvSet2 regulates fungal growth, pathogenicity, and secondary metabolism in Fusarium verticillioides. Fungal Genetics and Biology. 2017; 107 ():24-30.

Chicago/Turabian Style

Qin Gu; Zhenzhong Wang; Xiao Sun; Tiantian Ji; Hai Huang; Yang Yang; Hao Zhang; Hafiz Abdul Samad Tahir; Liming Wu; Huijun Wu; Xuewen Gao. 2017. "FvSet2 regulates fungal growth, pathogenicity, and secondary metabolism in Fusarium verticillioides." Fungal Genetics and Biology 107, no. : 24-30.

Journal article
Published: 02 August 2017 in BMC Plant Biology
Reads 0
Downloads 0

Microbial volatiles play an expedient role in the agricultural ecological system by enhancing plant growth and inducing systemic resistance against plant pathogens, without causing hazardous effects on the environment. To explore the effects of VOCs of Ralstonia solanacearum TBBS1 (Rs) on tobacco plant growth and on plant growth promoting efficiency of VOCs produced by Bacillus subtilis SYST2, experiments were conducted both in vitro and in planta. The VOCs produced by SYST2 significantly enhanced the plant growth and induced the systemic resistance (ISR) against wilt pathogen Rs in all experiments. The SYST2-VOCs significantly increased PPO and PAL activity and over-expressed the genes relating to expansin, wilt resistance, and plant defense while repressed the genes relating to ethylene production. More interestingly, VOCs produced by pathogen, Rs had no significant effect on plant growth; however, Rs-VOCs decreased the growth promoting potential of SYST2-VOCs when plants were exposed to VOCs produced by both SYST2 and Rs. The co-culture of SYST2 and Rs revealed that they inhibited the growth of each other; however, the inhibition of Rs by SYST2-VOCs appeared to be greater than that of SYST2 by Rs-VOCs. Our findings provide new insights regarding the interaction among SYST2-VOCs, Rs-VOCs and plant, resulting in growth promotion and induced systemic resistance against the bacterial wilt pathogen Rs. This is the first report of the effect of VOCs produced by pathogenic microorganism on plant growth and on plant growth-promoting and systemic resistance-inducing potential of PGPR strain SYST2.

ACS Style

Hafiz Abdul Samad Tahir; Qin Gu; Huijun Wu; Waseem Raza; Asma Safdar; Ziyang Huang; Faheem Uddin Rajer; Xuewen Gao. Effect of volatile compounds produced by Ralstonia solanacearum on plant growth promoting and systemic resistance inducing potential of Bacillus volatiles. BMC Plant Biology 2017, 17, 1 -16.

AMA Style

Hafiz Abdul Samad Tahir, Qin Gu, Huijun Wu, Waseem Raza, Asma Safdar, Ziyang Huang, Faheem Uddin Rajer, Xuewen Gao. Effect of volatile compounds produced by Ralstonia solanacearum on plant growth promoting and systemic resistance inducing potential of Bacillus volatiles. BMC Plant Biology. 2017; 17 (1):1-16.

Chicago/Turabian Style

Hafiz Abdul Samad Tahir; Qin Gu; Huijun Wu; Waseem Raza; Asma Safdar; Ziyang Huang; Faheem Uddin Rajer; Xuewen Gao. 2017. "Effect of volatile compounds produced by Ralstonia solanacearum on plant growth promoting and systemic resistance inducing potential of Bacillus volatiles." BMC Plant Biology 17, no. 1: 1-16.

Journal article
Published: 24 January 2017 in Toxins
Reads 0
Downloads 0

Fusarium verticillioides (teleomorph, Gibberella moniliformis) is an important plant pathogen that causes seedling blight, stalk rot, and ear rot in maize (Zea mays). During infection, F. verticillioides produce fumonsins B1 (FB1) that pose a serious threat to human and animal health. Recent studies showed that Set1, a methyltransferase of H3K4, was responsible for toxin biosynthesis in filamentous fungi. However, to date, the regulation of FvSet1 on FB1 biosynthesis remains unclear. In the current study, we identified only one Set1 ortholog in F. verticillioides (FvSet1) and found that the deletion of FvSET1 led to various defects in fungal growth and pathogenicity. More interestingly, the FvSET1 deletion mutant (ΔFvSet1) showed a significant defect in FB1 biosynthesis and lower expression levels of FUM genes. FvSet1 was also found to play an important role in the responses of F. verticillioides to multiple environmental stresses via regulating the phosphorylation of FvMgv1 and FvHog1. Taken together, these results indicate that FvSet1 plays essential roles in the regulation of FB1 biosynthesis, fungal growth and virulence, as well as various stress responses in F. verticillioides.

ACS Style

Qin Gu; Hafiz Abdul Samad Tahir; Hao Zhang; Hai Huang; Tiantian Ji; Xiao Sun; Liming Wu; Huijun Wu; Xuewen Gao. Involvement of FvSet1 in Fumonisin B1 Biosynthesis, Vegetative Growth, Fungal Virulence, and Environmental Stress Responses in Fusarium verticillioides. Toxins 2017, 9, 43 .

AMA Style

Qin Gu, Hafiz Abdul Samad Tahir, Hao Zhang, Hai Huang, Tiantian Ji, Xiao Sun, Liming Wu, Huijun Wu, Xuewen Gao. Involvement of FvSet1 in Fumonisin B1 Biosynthesis, Vegetative Growth, Fungal Virulence, and Environmental Stress Responses in Fusarium verticillioides. Toxins. 2017; 9 (2):43.

Chicago/Turabian Style

Qin Gu; Hafiz Abdul Samad Tahir; Hao Zhang; Hai Huang; Tiantian Ji; Xiao Sun; Liming Wu; Huijun Wu; Xuewen Gao. 2017. "Involvement of FvSet1 in Fumonisin B1 Biosynthesis, Vegetative Growth, Fungal Virulence, and Environmental Stress Responses in Fusarium verticillioides." Toxins 9, no. 2: 43.

Journal article
Published: 01 October 2015 in Enzyme and Microbial Technology
Reads 0
Downloads 0

A novel thermostable mannanase from a newly isolated Bacillus pumilus GBSW19 has been identified, expressed, purified and characterized. The enzyme shows a structure comprising a 28 amino acid signal peptide, a glycoside hydrolase family 5 (GH5) catalytic domain and no carbohydrate-binding module. The recombinant mannanase has molecular weight of 45 kDa with an optimal pH around 6.5 and is stable in the range from pH 5-11. Meanwhile, the optimal temperature is around 65 °C, and it retains 50% relative activity at 60 °C for 12h. In addition, the purified enzyme can be activated by several ions and organic solvents and is resistant to detergents. Bpman5 can efficiently convert locus bean gum to mainly M2, M3 and M5, and hydrolyze manno-oligosaccharides with a minimum DP of 3. Further exploration of the optimum condition using HPLC to prepare oligosaccharides from locust bean gum was obtained as 10mg/ml locust bean gum incubated with 10 U/mg enzyme at 50 °C for 24h. By using this enzyme, locust bean gum can be utilized to generate high value-added oligosaccharides with a DP of 2-6.

ACS Style

Haoyu Zang; Shanshan Xie; Huijun Wu; Weiduo Wang; Xiankun Shao; Liming Wu; Faheem Uddin Rajer; Xuewen Gao. A novel thermostable GH5_7 β-mannanase from Bacillus pumilus GBSW19 and its application in manno-oligosaccharides (MOS) production. Enzyme and Microbial Technology 2015, 78, 1 -9.

AMA Style

Haoyu Zang, Shanshan Xie, Huijun Wu, Weiduo Wang, Xiankun Shao, Liming Wu, Faheem Uddin Rajer, Xuewen Gao. A novel thermostable GH5_7 β-mannanase from Bacillus pumilus GBSW19 and its application in manno-oligosaccharides (MOS) production. Enzyme and Microbial Technology. 2015; 78 ():1-9.

Chicago/Turabian Style

Haoyu Zang; Shanshan Xie; Huijun Wu; Weiduo Wang; Xiankun Shao; Liming Wu; Faheem Uddin Rajer; Xuewen Gao. 2015. "A novel thermostable GH5_7 β-mannanase from Bacillus pumilus GBSW19 and its application in manno-oligosaccharides (MOS) production." Enzyme and Microbial Technology 78, no. : 1-9.

Journal article
Published: 06 February 2015 in BMC Microbiology
Reads 0
Downloads 0

Plant growth-promoting rhizobacteria (PGPR) are soil beneficial microorganisms that colonize plant roots for nutritional purposes and accordingly benefit plants by increasing plant growth or reducing disease. However, the mechanisms and pathways involved in the interactions between PGPR and plants remain unclear. In order to better understand these complex plant-PGPR interactions, changes in the transcriptome of the typical PGPR Bacillus subtilis in response to rice seedlings were analyzed. Microarray technology was used to study the global transcriptionl response of B. subtilis OKB105 to rice seedlings after an interaction period of 2 h. A total of 176 genes representing 3.8% of the B. subtilis strain OKB105 transcriptome showed significantly altered expression levels in response to rice seedlings. Among these, 52 were upregulated, the majority of which are involved in metabolism and transport of nutrients, and stress responses, including araA, ywkA, yfls, mtlA, ydgG et al. The 124 genes that were downregulated included cheV, fliL, spmA and tua, and these are involved in chemotaxis, motility, sporulation and teichuronic acid biosynthesis, respectively. We present a transcriptome analysis of the bacteria Bacillus subtilis OKB105 in response to rice seedings. Many of the 176 differentially expressed genes are likely to be involved in the interaction between Gram-positive bacteria and plants.

ACS Style

Shanshan Xie; Huijun Wu; Lina Chen; Haoyu Zang; Yongli Xie; Xuewen Gao. Transcriptome profiling of Bacillus subtilis OKB105 in response to rice seedlings. BMC Microbiology 2015, 15, 21 -14.

AMA Style

Shanshan Xie, Huijun Wu, Lina Chen, Haoyu Zang, Yongli Xie, Xuewen Gao. Transcriptome profiling of Bacillus subtilis OKB105 in response to rice seedlings. BMC Microbiology. 2015; 15 (1):21-14.

Chicago/Turabian Style

Shanshan Xie; Huijun Wu; Lina Chen; Haoyu Zang; Yongli Xie; Xuewen Gao. 2015. "Transcriptome profiling of Bacillus subtilis OKB105 in response to rice seedlings." BMC Microbiology 15, no. 1: 21-14.