Anthony Purcell

Prof. Anthony Purcell

Monash University, Department of Biochemistry & Molecular Biology
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orcid

Research Focus & Metrics

Academic Career Length

38Years (1989-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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10 October 2012 in Nature
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Journal Article
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LKLars Kjer-Nielsen
Lars Kjer-Nielsen
OPOnisha Patel
Onisha Patel
ACAlexandra Corbett
Alexandra Corbett
JNJerome Le Nours
Jerome Le Nours
BMBronwyn Meehan
Bronwyn Meehan
JMJames McCluskey
James McCluskey

30 March 2011 in Nature
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Journal Article
Closed access
BGBjörn Gerlach
Björn Gerlach
SCStefanie M. Cordier
Stefanie M. Cordier
ASAnna C. Schmukle
Anna C. Schmukle
CEChristoph H. Emmerich
Christoph H. Emmerich
EREva Rieser
Eva Rieser
HWHenning Walczak
Henning Walczak

1 May 2007 in Nature Reviews Drug Discovery
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Journal Article
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Anthony PurcellAnthony Purcell
Anthony Purcell
JMJames McCluskey
James McCluskey
JRJamie Rossjohn
Jamie Rossjohn

23 May 2012 in Nature
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Journal Article
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PIPatricia Illing
Patricia Illing
JVJulian P. Vivian
Julian P. Vivian
NDNadine L. Dudek
Nadine L. Dudek
LKLyudmila Kostenko
Lyudmila Kostenko
ZCZhenjun Chen
Zhenjun Chen
JMJames McCluskey
James McCluskey

15 May 2019 in Nature Protocols
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Journal Article
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Anthony PurcellAnthony Purcell
Anthony Purcell
SRSri H. Ramarathinam
Sri H. Ramarathinam
NTNicola Ternette
Nicola Ternette

4 November 2013 in Journal of Experimental Medicine
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Journal Article
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SSStephen W. Scally
Stephen W. Scally
JPJan Petersen
Jan Petersen
SLSoi Cheng Law
Soi Cheng Law
NDNadine L. Dudek
Nadine L. Dudek
HNHendrik J. Nel
Hendrik J. Nel
JRJamie Rossjohn
Jamie Rossjohn
Rheumatoid arthritis (RA) is strongly associated with the human leukocyte antigen (HLA)-DRB1 locus that possesses the shared susceptibility epitope (SE) and the citrullination of self-antigens. We show how citrullinated aggrecan and vimentin epitopes bind to HLA-DRB1*04:01/04. Citrulline was accommodated within the electropositive P4 pocket of HLA-DRB1*04:01/04, whereas the electronegative P4 pocket of the RA-resistant HLA-DRB1*04:02 allomorph interacted with arginine or citrulline-containing epitopes. Peptide elution studies revealed P4 arginine–containing peptides from HLA-DRB1*04:02, but not from HLA-DRB1*04:01/04. Citrullination altered protease susceptibility of vimentin, thereby generating self-epitopes that are presented to T cells in HLA-DRB1*04:01+ individuals. Using HLA-II tetramers, we observed citrullinated vimentin- and aggrecan-specific CD4+ T cells in the peripheral blood of HLA-DRB1*04:01+ RA-affected and healthy individuals. In RA patients, autoreactive T cell numbers correlated with disease activity and were deficient in regulatory T cells relative to healthy individuals. These findings reshape our understanding of the association between citrullination, the HLA-DRB1 locus, and T cell autoreactivity in RA.