Nitrogen fertilizer is an important component of crop production; however, its excessive application could result in N loss that could have serious environmental concerns. Straw incorporation in the soil after crop harvest is one of the most feasible straw management techniques, however, the optimization of nitrogen (N) fertilizer management and maize straw incorporation to modulate the crop yield and to maintain N balance in sweet corn is necessary to get better yields on a sustained basis. The present study was comprised of two straw management treatments i.e., (i) no straw return (S0), and (ii) incorporation of crushed corn ears by into the soil using rotary tiller (S100) and four N fertilizer management treatments i.e., (i) common farmer practice with total N applied at 300 kg ha⁻¹ with 50%, 5%, 30%, and 15% splits at basal, 3-leaf stage (V3), at hilling stage (V8) and at tasseling/flowering stage (RT), respectively (FM); (ii) application of total N at 225 kg ha⁻¹ with 40%, 10% and 50% at basal, 3-leaf stage (V3) and at hilling stage (V8), respectively (OMI); (iii) application of total N at 150 kg ha⁻¹ with 40%, 10% and 50% at basal, 3-leaf stage (V3), and at hilling stage (V8), respectively (OMII); and (iv) the treatment without any fertilizer application (N0). The hybrid sweet corn cultivar ‘Yuetian 28’ was grown during the spring and autumn growing seasons of 2016 and 2017. The results showed that the N management treatments substantially improved the fresh ear yield, ear number and ear weight, partial factor productivity from nitrogen (PFP_N), recovery efficiency from nitrogen (RE_N), and agronomic efficiency from nitrogen (AE_N). Compared with FM, the OMI and OMII treatments significantly increased the AE_N and PFP_N whilst decreased the apparent N loss, however, the fresh ear yield, ear number and ear weight in OMI treatment were found to be statistically similar (p ˃ 0.05) to FM. Moreover, the S100 treatment did not affect the fresh ear yield and yield components significantly. Overall, straw return combined with an optimized N fertilizer application could improve the yield of sweet corn on a sustained basis with minimum and/or negligible N loss.

In plants, sugar metabolism involves a complex interplay of genetic, molecular and environmental factors. To better understand the molecular mechanisms underlying these processes, we utilized a multi-layered approach that integrated transcriptomic and metabolomic datasets generated from multiple different varieties of sweet corn. Through this analysis, we found 2533 genes that were differentially expressed in the immature kernel tissues of sweet corn, including genes involved in transcriptional regulation, sugar metabolism, primary metabolism, and other processes associated with adaptability of sweet corn. We also detected 31 differential metabolites among the three types of sweet corn. Utilizing an integrated approach encompassing transcriptomics and eGWAS, we elucidated the transcriptional regulatory patterns governing these differential metabolites. Specifically, we delved into the transcriptional modulation of malate- and ubiquitin-associated genes across a range of sweet corn varieties, shedding new light on the molecular mechanisms underlying their regulation. This study provides a framework for future research aimed at improving the current understanding of sugar metabolism and regulatory gene networks in sweet corn, which could ultimately lead to the development of novel strategies for crop improvement.