Reconstructing the colonization and demographic dynamics that gave rise to extant forests is essential to forecasts of forest responses to environmental changes. Classical approaches to map how population of trees changed through space and time largely rely on pollen distribution patterns, with only a limited number of studies exploiting DNA molecules preserved in wooden tree archaeological and subfossil remains. Here, we advance such analyses by applying high‐throughput (HTS) DNA sequencing to wood archaeological and subfossil material for the first time, using a comprehensive sample of 167 European white oak waterlogged remains spanning a large temporal (from 550 to 9,800 years) and geographical range across Europe. The successful characterization of the endogenous DNA and exogenous microbial DNA of 140 (~83%) samples helped the identification of environmental conditions favouring long‐term DNA preservation in wood remains, and started to unveil the first trends in the DNA decay process in wood material. Additionally, the maternally inherited chloroplast haplotypes of 21 samples from three periods of forest human‐induced use (Neolithic, Bronze Age and Middle Ages) were found to be consistent with those of modern populations growing in the same geographic areas. Our work paves the way for further studies aiming at using ancient DNA preserved in wood to reconstruct the micro‐evolutionary response of trees to climate change and human forest management.
1 October 2015 in Palaeogeography, Palaeoclimatology, Palaeoecology
The study of the technology underlying pre-industrial storage structures has an interest from an anthropological and archaeological perspective, in terms of the evolution of key cultural and cognitive capabilities, often related to the transition to food production.\ud \ud Microarchaeological techniques offer a unique perspective on the study of pre-industrial storing technologies. In this work, examples are presented from two archaeological contexts in different climatic and socio-ecological situations during the Holocene in S Asia and SW Europe. Microarchaeological techniques used in this study include micromorphology, Fourier transform infrared spectroscopy and phytolith analyses. The comparative study of two pits highlights key aspects of the decision-making process involved in technological solutions of storage: \ud • \ud \ud The choice of a location for the construction of a given storage facility is highly affected by contextual climatic, microclimatic, soil and bioturbative factors\ud • \ud \ud The time taken to consume stored foodstuffs seems to affect technological investment as much as the intrinsic conservation requirements of the stored taxa\ud • \ud \ud The use of fire to hygienise pits implies that such structures were not conceived for single use\ud • \ud \ud Pre-industrial storage systems can be seen as modular structures, which components (e.g. topographical location, sediment type, lining type, hygienisation techniques and cover) can be recombined to improve storage performance for different climatic settings and foodstuffs.Peer reviewe
Located on the Iberian Mediterranean coast, El Collado is an open-air site where a rescue excavation was conducted over two seasons in 1987 and 1988. The archaeological work excavated a surface area of 143m2 where 14 burials were discovered, providing skeletal remains from 15 individuals. We have obtained AMS dates for 10 of the 15 individuals by means of the direct dating of human bones. The ranges of the probability distribution of the calibrated dates suggest that the cemetery was used during a long period of time (781–1020 years at a probability of 95.4%). The new dates consequently set back the chrono-cultural attribution of the cemetery from the initial proposal of Late Mesolithic to an older date in the Early Mesolithic. Therefore, El Collado becomes the oldest known cemetery in the Iberian Peninsula, earlier than the numerous Mesolithic funerary contexts documented on the Atlantic façade such as the Portuguese shell-middens in the Muge and Sado Estuaries or the funerary sites on the northern Iberian coast.