Heavy metal monitoring in food-producing ecosystems can play an important role in human health safety. Since they are able to interfere with plants’ physiochemical characteristics, which influence the optical properties of leaves, they can be measured by in-field spectroscopy. In this study, the predictive power of spectroscopic data is examined. Five treatments of heavy metal stress (Cu, Zn, Pb, Cr, and Cd) were applied to grapevine seedlings and hyperspectral data (350–2500 nm), and heavy metal contents were collected based on in-field and laboratory experiments. The partial least squares (PLS) method was used as a feature selection technique, and multiple linear regressions (MLR) and support vector machine (SVM) regression methods were applied for modelling purposes. Based on the PLS results, the wavelengths in the vicinity of 2431, 809, 489, and 616 nm; 2032, 883, 665, 564, 688, and 437 nm; 1865, 728, 692, 683, and 356 nm; 863, 2044, 415, 652, 713, and 1036 nm; and 1373, 631, 744, and 438 nm were found most sensitive for the estimation of Cu, Zn, Pb, Cr, and Cd contents in the grapevine leaves, respectively. Therefore, visible and red-edge regions were found most suitable for estimating heavy metal contents in the present study. Heavy metals played a significant role in reforming the spectral pattern of stressed grapevine compared to healthy samples, meaning that in the best structures of the SVM regression models, the concentrations of Cu, Zn, Pb, Cr, and Cd were estimated with R² rates of 0.56, 0.85, 0.71, 0.80, and 0.86 in the testing set, respectively. The results confirm the efficiency of in-field spectroscopy in estimating heavy metals content in grapevine foliage.

Sustainable management of orchard fields requires detailed information about the tree types, which is a main component of precision agriculture programs. To this end, hyperspectral imagery can play a major role in orchard tree species mapping. Efficient use of hyperspectral data in combination with field measurements requires the development of optimized band selection strategies to separate tree species. In this study, field spectroscopy (350 to 2500 nm) was performed through scanning 165 spectral leaf samples of dominant orchard tree species (almond, walnut, and grape) in Chaharmahal va Bakhtiyari province, Iran. Two multivariable methods were employed to identify the optimum wavelengths: the first includes three-step approach ANOVA, random forest classifier (RFC) and principal component analysis (PCA), and the second employs partial least squares (PLS). For both methods we determined whether tree species can be spectrally separated using discriminant analysis (DA) and then the optimal wavelengths were identified for this purpose. Results indicate that all species express distinct spectral behaviors at the beginning of the visible range (from 350 to 439 nm), the red edge and the near infrared wavelengths (from 701 to 1405 nm). The ANOVA test was able to reduce primary wavelengths (2151) to 792, which had a significant difference (99% confidence level), then the RFC further reduced the wavelengths to 118. By removing the overlapping wavelengths, the PCA represented five components (99.87% of variance) which extracted optimal wavelengths were: 363, 423, 721, 1064, and 1388 nm. The optimal wavelengths for the species discrimination using the best PLS-DA model (100% accuracy) were at 397, 515, 647, 1386, and 1919 nm.