OBJECTIVE—SIRT1, a class III histone/protein deacetylase, is known to interfere with the nuclear factor-κB (NF-κB) signaling pathway and thereby has an anti-inflammatory function. Because of the central role of NF-κB in cytokine-mediated pancreatic β-cell damage, we postulated that SIRT1 might work in pancreatic β-cell damage models. RESEARCH DESIGN AND METHODS—RINm5F (RIN) cells or isolated rat islets were treated with interleukin-1β and interferon-γ. SIRT1 was activated by resveratrol, a pharmacological activator, or ectopic overexpression. The underlying mechanisms of SIRT1 against cytokine toxicity were further explored. RESULTS—Treatment of RIN cells with cytokines induced cell damage, and this damage was well correlated with the expression of the inducible form of nitric oxide (NO) synthase (iNOS) and NO production. However, SIRT1 overexpression completely prevented cytokine-mediated cytotoxicity, NO production, and iNOS expression. The molecular mechanism by which SIRT1 inhibits iNOS expression appeared to involve the inhibition of the NF-κB signaling pathway through deacetylation of p65. In addition, SIRT1 activation by either resveratrol or adenoviral-directed overexpression of SIRT1 could prevent cytokine toxicity and maintain normal insulin-secreting responses to glucose in isolated rat islets. CONCLUSIONS—This study will provide valuable information not only into the mechanisms underlying β-cell destruction but also into the regulation of SIRT1 as a possible target to attenuate cytokine-induced β-cell damage.

Novel biodegradable poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [PHBV]/graphene nanocomposites were prepared by solution casting. The thermal properties, crystallization behavior, microstructure, and fracture morphology of the composites were investigated. Scanning electron microscope (SEM) results show that graphene layers are homogeneously dispersed in the polymer matrix. X-ray diffraction (XRD) and dynamic scanning calorimetry (DSC) studies show that the well dispersed graphene sheets act as nucleating agent for crystallization. Consequently, the mechanical properties of the composites have been substantially improved as evident from dynamic mechanical and static tensile tests. Differential thermal analysis (DTA) showed an increase in temperature of maximum degradation. Soil degradation tests of PHBV/graphene nanocomposites showed that presence of graphene doesn’t interfere in its biodegradability

In this study, the environmental impacts of various alternative ship fuels for a coastal ferry were assessed by the life cycle assessment (LCA) analysis. The comparative study was performed with marine gas oil (MGO), natural gas, and hydrogen with various energy sources for a 12,000 gross tonne (GT) coastal ferry operating in the Republic of Korea (ROK). Considering the energy imports of ROK, i.e., MGO from Saudi Arabia and natural gas from Qatar, these countries were chosen to provide the MGO and the natural gas for the LCA. The hydrogen is considered to be produced by steam methane reforming (SMR) from natural gas with hard coal, nuclear energy, renewable energy, and electricity in the ROK model. The lifecycles of the fuels were analyzed in classifications of Well-to- Tank, Tank-to-Wake, and Well-to-Wake phases. The environmental impacts were provided in terms of global warming potential (GWP), acidification potential (AP), photochemical potential (POCP), eutrophication potential (EP), and particulate matter (PM). The results showed that MGO and natural gas cannot be used for ships to meet the International Maritime Organization’s (IMO) 2050 GHG regulation. Moreover, it was pointed out that the energy sources in SMR are important contributing factors to emission levels. The paper concludes with suggestions for a hydrogen application plan for ships from small, nearshore ships in order to truly achieve a ship with zero emissions based on the results of this study.

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Nerve growth factor (NGF) is a neurotrophin and has been suggested to induce heme oxygenase-1 (HO1) expression. Although the role of HO1 in tumorigenesis remains controversial, recent evidence suggests NGF and HO1 as tumor-progressing factors. However, the correlative role of NGF and HO1 and their prognostic impact in breast carcinoma is unknown.