Objective: Targeting the protein of interest to a particular tissue to achieve high-level expression is an important strategy to increase expression efficiency. The use of the plant seed oil body as a bioreactor can not only increase the amount of target protein, but also reduce the cost of downstream processing. Methods: VEGF ₁₆₅ was expressed in Arabidopsis thaliana seeds via oilbody fusion technology. The pKO-VEGF ₁₆₅ vector was construted and transformed into A. thaliana seeds. T3 transgenic seeds was detected by SDS-PAGE and western blot methods. The cell activity was tested by MTT methods. Result: The phaseolin promoter was used to drive seed-specific expression of the VEGF ₁₆₅ gene in transgenic A. thaliana . The coding region of VEGF ₁₆₅ was fused to the Arabidopsis oleosin sequence to target the protein to the oil bodies in the seeds of transgenic plants. The T-DNA region of recombinant plasmid pKO-VEGF ₁₆₅ was shifted to A. thaliana seeds via the floral-dip method. Protein was analyzed by electrophoresis and protein hybridization analyses. Finally, MTT assays showed that the oleosin-VEGF ₁₆₅ fusion protein played a part in the proliferation of HUVEC cells in vitro. Conclusion: Oleosin-VEGF ₁₆₅ was successfully expressed and it had stimulated HUVEC cell proliferation activity.