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Seanna L. Hewitt
Department of Horticulture, Washington State University, Pullman, WA 99163, USA

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Journal article
Published: 30 August 2021 in Horticulturae
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The harvesting of sweet cherry (Prunus avium L.) fruit is a labor-intensive process. The mechanical harvesting of sweet cherry fruit is feasible; however, it is dependent on the formation of an abscission zone at the fruit–pedicel junction. The natural propensity for pedicel-–fruit abscission zone (PFAZ) activation varies by cultivar, and the general molecular basis for PFAZ activation is not well characterized. In this study, ethylene-inducible change in pedicel fruit retention force (PFRF) was recorded in a developmental time-course with a concomitant analysis of the PFAZ transcriptome from three sweet cherry cultivars. In ‘Skeena’, mean PFRF for both control and treatment fruit dropped below the 0.40 kg-force (3.92 N) threshold for mechanical harvesting, indicating the activation of a discrete PFAZ. In ‘Bing’, mean PFRF for both control and treatment groups decreased over time. However, a mean PFRF conducive to mechanical harvesting was achieved only in the ethylene-treated fruit. While in ‘Chelan’ the mean PFRF of the control and treatment groups did not meet the threshold required for efficient mechanical harvesting. Transcriptome analysis of the PFAZ region followed by the functional annotation, differential expression analysis, and gene ontology (GO) enrichment analyses of the data facilitated the identification of phytohormone-responsive and abscission-related transcripts, as well as processes that exhibited differential expression and enrichment in a cultivar-dependent manner over the developmental time-course. Additionally, read alignment-based variant calling revealed several short variants in differentially expressed genes, associated with enriched gene ontologies and associated metabolic processes, lending potential insight into the genetic basis for different abscission responses between the cultivars. These results provide genetic targets for the induction or inhibition of PFAZ activation, depending on the desire to harvest the fruit with or without the stem attached. Understanding the genetic mechanisms underlying the development of the PFAZ will inform future cultivar development while laying a foundation for mechanized sweet cherry harvest.

ACS Style

SeAnna Hewitt; Benjamin Kilian; Tyson Koepke; Jonathan Abarca; Matthew Whiting; Amit Dhingra. Transcriptome Analysis Reveals Potential Mechanisms for Ethylene-Inducible Pedicel–Fruit Abscission Zone Activation in Non-Climacteric Sweet Cherry (Prunus avium L.). Horticulturae 2021, 7, 270 .

AMA Style

SeAnna Hewitt, Benjamin Kilian, Tyson Koepke, Jonathan Abarca, Matthew Whiting, Amit Dhingra. Transcriptome Analysis Reveals Potential Mechanisms for Ethylene-Inducible Pedicel–Fruit Abscission Zone Activation in Non-Climacteric Sweet Cherry (Prunus avium L.). Horticulturae. 2021; 7 (9):270.

Chicago/Turabian Style

SeAnna Hewitt; Benjamin Kilian; Tyson Koepke; Jonathan Abarca; Matthew Whiting; Amit Dhingra. 2021. "Transcriptome Analysis Reveals Potential Mechanisms for Ethylene-Inducible Pedicel–Fruit Abscission Zone Activation in Non-Climacteric Sweet Cherry (Prunus avium L.)." Horticulturae 7, no. 9: 270.

Genome editing in plants
Published: 06 April 2021 in Transgenic Research
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The advent of genome editing has opened new avenues for targeted trait enhancement in fruit, ornamental, industrial, and all specialty crops. In particular, CRISPR-based editing systems, derived from bacterial immune systems, have quickly become routinely used tools for research groups across the world seeking to edit plant genomes with a greater level of precision, higher efficiency, reduced off-target effects, and overall ease-of-use compared to ZFNs and TALENs. CRISPR systems have been applied successfully to a number of horticultural and industrial crops to enhance fruit ripening, increase stress tolerance, modify plant architecture, control the timing of flower development, and enhance the accumulation of desired metabolites, among other commercially-important traits. As editing technologies continue to advance, so too does the ability to generate improved crop varieties with non-transgenic modifications; in some crops, direct transgene-free edits have already been achieved, while in others, T-DNAs have successfully been segregated out through crossing. In addition to the potential to produce non-transgenic edited crops, and thereby circumvent regulatory impediments to the release of new, improved crop varieties, targeted gene editing can speed up trait improvement in crops with long juvenile phases, reducing inputs resulting in faster market introduction to the market. While many challenges remain regarding optimization of genome editing in ornamental, fruit, and industrial crops, the ongoing discovery of novel nucleases with niche specialties for engineering applications may form the basis for additional and potentially crop-specific editing strategies.

ACS Style

Fabiola Ramirez-Torres; Rishikesh Ghogare; Evan Stowe; Pedro CerdĂĄ-Bennasser; Maria Lobato-GĂłmez; Bruce A Williamson-Benavides; Patricia Sarai Giron-Calva; SeAnna Hewitt; Paul Christou; Amit Dhingra. Genome editing in fruit, ornamental, and industrial crops. Transgenic Research 2021, 30, 499 -528.

AMA Style

Fabiola Ramirez-Torres, Rishikesh Ghogare, Evan Stowe, Pedro CerdĂĄ-Bennasser, Maria Lobato-GĂłmez, Bruce A Williamson-Benavides, Patricia Sarai Giron-Calva, SeAnna Hewitt, Paul Christou, Amit Dhingra. Genome editing in fruit, ornamental, and industrial crops. Transgenic Research. 2021; 30 (4):499-528.

Chicago/Turabian Style

Fabiola Ramirez-Torres; Rishikesh Ghogare; Evan Stowe; Pedro CerdĂĄ-Bennasser; Maria Lobato-GĂłmez; Bruce A Williamson-Benavides; Patricia Sarai Giron-Calva; SeAnna Hewitt; Paul Christou; Amit Dhingra. 2021. "Genome editing in fruit, ornamental, and industrial crops." Transgenic Research 30, no. 4: 499-528.

Preprint content
Published: 07 September 2020
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Harvesting of sweet cherry (Prunus avium L.) fruit is a labor-intensive process. Mechanical harvesting of sweet cherry fruit is feasible; however, it is dependent on the formation of an abscission zone at the fruit-pedicel junction. The natural propensity for pedicel-fruit abscission zone (PFAZ) formation varies by cultivar, and the general molecular basis for PFAZ formation is not well characterized. In this study, ethylene-inducible change in pedicel fruit retention force (PFRF) was recorded in a developmental time course with a concomitant analysis of the PFAZ transcriptome from three sweet cherry cultivars. In ‘Skeena’, mean PFRF for both control and treatment fruit dropped below the 0.40kg-force (3.92N) threshold for mechanical harvesting and indicating the formation of a discrete PFAZ. In ‘Bing’, mean PFRF for both control and treatment groups decreased over time. However, a mean PFRF conducive to mechanical harvesting was achieved only in the ethylene-treated fruit. While in ‘Chelan’ the mean PFRF of the control and treatment groups did not meet the threshold required for efficient mechanical harvesting. Transcriptome analysis of the PFAZ followed by the functional annotation, differential expression analysis, and gene ontology (GO) enrichment analyses of the data facilitated the identification of phytohormone-responsive and abscission-related transcripts as well as processes that exhibited differential expression and enrichment in a cultivar-dependent manner over the developmental time-course. Additionally, read alignment-based variant calling revealed several short variants in differentially expressed genes, associated with enriched gene ontologies and associated metabolic processes, lending potential insight into the genetic basis for different abscission responses between the cultivars. These results provide genetic targets for induction or inhibition of PFAZ formation, depending on the desire to harvest the fruit with or without the stem attached. Understanding the genetic mechanisms underlying the development of the PFAZ will inform future cultivar development while laying a foundation for mechanized sweet cherry harvest.

ACS Style

Seanna L Hewitt; Benjamin Kilian; Tyson Koepke; Jonathan Abarca; Matthew Whiting; Amit Dhingra. Characterization of ethylene-inducible pedicel-fruit abscission zone formation in non-climacteric sweet cherry (Prunus avium L.). 2020, 1 .

AMA Style

Seanna L Hewitt, Benjamin Kilian, Tyson Koepke, Jonathan Abarca, Matthew Whiting, Amit Dhingra. Characterization of ethylene-inducible pedicel-fruit abscission zone formation in non-climacteric sweet cherry (Prunus avium L.). . 2020; ():1.

Chicago/Turabian Style

Seanna L Hewitt; Benjamin Kilian; Tyson Koepke; Jonathan Abarca; Matthew Whiting; Amit Dhingra. 2020. "Characterization of ethylene-inducible pedicel-fruit abscission zone formation in non-climacteric sweet cherry (Prunus avium L.)." , no. : 1.

Journal article
Published: 21 May 2020 in Scientific Reports
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European pear (Pyrus communis L.) cultivars require a genetically pre-determined duration of cold-temperature exposure to induce autocatalytic system 2 ethylene biosynthesis and subsequent fruit ripening. The physiological responses of pear to cold-temperature-induced ripening have been well characterized, but the molecular mechanisms underlying this phenomenon continue to be elucidated. This study employed previously established cold temperature conditioning treatments for ripening of two pear cultivars, ‘D’Anjou’ and ‘Bartlett’. Using a time-course transcriptomics approach, global gene expression responses of each cultivar were assessed at four stages of developmental during the cold conditioning process. Differential expression, functional annotation, and gene ontology enrichment analyses were performed. Interestingly, evidence for the involvement of cold-induced, vernalization-related genes and repressors of endodormancy release was found. These genes have not previously been described to play a role in fruit during the ripening transition. The resulting data provide insight into cultivar-specific mechanisms of cold-induced transcriptional regulation of ripening in European pear, as well as a unique comparative analysis of the two cultivars with very different cold conditioning requirements.

ACS Style

SeAnna Hewitt; Christopher A. Hendrickson; Amit Dhingra. Evidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit. Scientific Reports 2020, 10, 1 .

AMA Style

SeAnna Hewitt, Christopher A. Hendrickson, Amit Dhingra. Evidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit. Scientific Reports. 2020; 10 (1):1.

Chicago/Turabian Style

SeAnna Hewitt; Christopher A. Hendrickson; Amit Dhingra. 2020. "Evidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit." Scientific Reports 10, no. 1: 1.

Journal article
Published: 19 May 2020 in Scientific Reports
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Plastids are morphologically and functionally diverse organelles that are dependent on nuclear-encoded, plastid-targeted proteins for all biochemical and regulatory functions. However, how plastid proteomes vary temporally, spatially, and taxonomically has been historically difficult to analyze at a genome-wide scale using experimental methods. A bioinformatics workflow was developed and evaluated using a combination of fast and user-friendly subcellular prediction programs to maximize performance and accuracy for chloroplast transit peptides and demonstrate this technique on the predicted proteomes of 15 sequenced plant genomes. Gene family grouping was then performed in parallel using modified approaches of reciprocal best BLAST hits (RBH) and UCLUST. A total of 628 protein families were found to have conserved plastid targeting across angiosperm species using RBH, and 828 using UCLUST. However, thousands of clusters were also detected where only one species had predicted plastid targeting, most notably in Panicum virgatum which had 1,458 proteins with species-unique targeting. An average of 45% overlap was found in plastid-targeted protein-coding gene families compared with Arabidopsis, but an additional 20% of proteins matched against the full Arabidopsis proteome, indicating a unique evolution of plastid targeting. Neofunctionalization through subcellular relocalization is known to impart novel biological functions but has not been described before on a genome-wide scale for the plastid proteome. Further work to correlate these predicted novel plastid-targeted proteins to transcript abundance and high-throughput proteomics will uncover unique aspects of plastid biology and shed light on how the plastid proteome has evolved to influence plastid morphology and biochemistry.

ACS Style

Ryan W. Christian; SeAnna Hewitt; Eric Roalson; Amit Dhingra. Genome-Scale Characterization of Predicted Plastid-Targeted Proteomes in Higher Plants. Scientific Reports 2020, 10, 1 -22.

AMA Style

Ryan W. Christian, SeAnna Hewitt, Eric Roalson, Amit Dhingra. Genome-Scale Characterization of Predicted Plastid-Targeted Proteomes in Higher Plants. Scientific Reports. 2020; 10 (1):1-22.

Chicago/Turabian Style

Ryan W. Christian; SeAnna Hewitt; Eric Roalson; Amit Dhingra. 2020. "Genome-Scale Characterization of Predicted Plastid-Targeted Proteomes in Higher Plants." Scientific Reports 10, no. 1: 1-22.

Article
Published: 27 April 2020 in Scientific Reports
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1-methylcyclopropene (1-MCP) in an ethylene receptor antagonist that blocks ethylene perception and downstream ripening responses in climacteric fruit imparting a longer shelf life. However, in European pear, the application of 1-MCP irreversibly obstructs the onset of system 2 ethylene production resulting in perpetually unripe fruit with undesirable quality. Application of exogenous ethylene, carbon dioxide and treatment to high temperatures is not able to reverse the blockage in ripening. We recently reported that during cold conditioning, activation of alternative oxidase (AOX) occurs pre-climacterically. In this study, we report that activation of AOX via exposure of 1-MCP treated ‘D’Anjou’ pear fruit to glyoxylic acid triggers an accelerated ripening response. Time course physiological analysis revealed that ripening is evident from decreased fruit firmness and increased internal ethylene. Transcriptomic and functional enrichment analyses revealed genes and ontologies implicated in glyoxylic acid-mediated ripening, including AOX, TCA cycle, fatty acid metabolism, amino acid metabolism, organic acid metabolism, and ethylene-responsive pathways. These observations implicate the glyoxylate cycle as a biochemical hub linking multiple metabolic pathways to stimulate ripening through an alternate mechanism. The results provide information regarding how blockage caused by 1-MCP may be circumvented at the metabolic level, thus opening avenues for consistent ripening in pear and possibly other fruit.

ACS Style

Seanna L. Hewitt; Rishikesh Ghogare; Amit Dhingra. Glyoxylic acid overcomes 1-MCP-induced blockage of fruit ripening in Pyrus communis L. var. ‘D’Anjou’. Scientific Reports 2020, 10, 1 -14.

AMA Style

Seanna L. Hewitt, Rishikesh Ghogare, Amit Dhingra. Glyoxylic acid overcomes 1-MCP-induced blockage of fruit ripening in Pyrus communis L. var. ‘D’Anjou’. Scientific Reports. 2020; 10 (1):1-14.

Chicago/Turabian Style

Seanna L. Hewitt; Rishikesh Ghogare; Amit Dhingra. 2020. "Glyoxylic acid overcomes 1-MCP-induced blockage of fruit ripening in Pyrus communis L. var. ‘D’Anjou’." Scientific Reports 10, no. 1: 1-14.

Preprint content
Published: 06 December 2019
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Plastids are morphologically and functionally diverse organelles that are dependent on nuclear-encoded, plastid-targeted proteins for all biochemical and regulatory functions. However, how plastid proteomes vary temporally, spatially, and taxonomically has been historically difficult to analyze at genome-wide scale using experimental methods. A bioinformatics workflow was developed and evaluated using a combination of fast and user-friendly subcellular prediction programs to maximize performance and accuracy for chloroplast transit peptides and demonstrate this technique on the predicted proteomes of 15 sequenced plant genomes. Gene family grouping was then performed in parallel using modified approaches of reciprocal best BLAST hits (RBH) and UCLUST. Between 628 protein families were found to have conserved plastid targeting across angiosperm species using RBH, and 828 using UCLUST. However, thousands of clusters were also detected where only one species had predicted plastid targeting, most notably inPanicum virgatumwhich had 1,458 proteins with species-unique targeting. An average of 45% overlap was found in plastid-targeted gene families compared with Arabidopsis, but an additional 20% of proteins matched against the full Arabidopsis proteome, indicating a unique evolution of plastid targeting. Neofunctionalization through subcellular relocalization is known to impart novel biological functions but has not been described before on genome-wide scale for the plastid proteome. Further work to correlate these predicted novel plastid-targeted proteins to transcript abundance and high-throughput proteomics will uncover unique aspects of plastid biology and shed light on how the plastid proteome has evolved to change plastid morphology and biochemistry.

ACS Style

Ryan W. Christian; Seanna Louise Hewitt; Eric H. Roalson; Amit Dhingra. Genome-Scale Characterization of Predicted Plastid-Targeted Proteomes in Higher Plants. 2019, 867242 .

AMA Style

Ryan W. Christian, Seanna Louise Hewitt, Eric H. Roalson, Amit Dhingra. Genome-Scale Characterization of Predicted Plastid-Targeted Proteomes in Higher Plants. . 2019; ():867242.

Chicago/Turabian Style

Ryan W. Christian; Seanna Louise Hewitt; Eric H. Roalson; Amit Dhingra. 2019. "Genome-Scale Characterization of Predicted Plastid-Targeted Proteomes in Higher Plants." , no. : 867242.

Preprint content
Published: 25 November 2019
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1-methylcyclopropene (1-MCP) in an ethylene receptor antagonist which blocks ethylene perception and downstream ripening responses in climacteric fruit imparting a longer shelf life. However, in European pear, application of 1-MCP irreversibly obstructs the onset of system 2 ethylene production resulting in perpetually unripe fruit with undesirable quality. Application of exogenous ethylene, carbon dioxide and treatment to high temperatures is not able to reverse the blockage in ripening. We recently reported that during cold conditioning, activation of AOX occurs pre-climaterically. In this study we report that activation of AOX via exposure of 1-MCP treated ‘D’Anjou’ pear fruit to glyoxylic acid triggers an accelerated ripening response. Ripening is consistently evident in decrease of fruit firmness and onset of S1-S2 ethylene transition. Time course ripening related measurements and transcriptomic analysis were performed to assess the effects of glyoxylic acid-driven stimulation of ripening. Transcriptomic and functional enrichment analyses revealed genes and ontologies implicated in glyoxylic acid mediated ripening, including alternative oxidase, TCA cycle, fatty acid metabolism, amino acid metabolism, organic acid metabolism, and ethylene responsive pathways. These observations implicate the glyoxylate cycle as a metabolic hub linking multiple pathways to stimulate ripening through an alternate mechanism. The results provide information regarding how blockage caused by 1-MCP may be circumvented at the metabolic level, thus opening avenues for consistent ripening in pear and possibly other fruit.

ACS Style

Seanna Louise Hewitt; Amit Dhingra. Glyoxylic acid overcomes 1-MCP-induced blockage of fruit ripening in Pyrus communis L. var. ‘D’Anjou’. 2019, 852954 .

AMA Style

Seanna Louise Hewitt, Amit Dhingra. Glyoxylic acid overcomes 1-MCP-induced blockage of fruit ripening in Pyrus communis L. var. ‘D’Anjou’. . 2019; ():852954.

Chicago/Turabian Style

Seanna Louise Hewitt; Amit Dhingra. 2019. "Glyoxylic acid overcomes 1-MCP-induced blockage of fruit ripening in Pyrus communis L. var. ‘D’Anjou’." , no. : 852954.

Preprint content
Published: 22 November 2019
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European pear (Pyrus communis L.) cultivars require a genetically pre-determined duration of cold-temperature exposure to induce autocatalytic system 2 ethylene biosynthesis and subsequent fruit ripening. The physiological responses of pear to cold-temperature-induced ripening have been well characterized, but the molecular mechanisms underlying this phenomenon continue to be elucidated. This study employed established cold temperature conditioning treatments for ripening of two pear cultivars, ‘D’Anjou’ and ‘Bartlett’. Using a time-course transcriptomics approach, global gene expression responses of each cultivar were assessed at four different developmental stages during the cold conditioning process. Differential expression, functional annotation, and gene ontology enrichment analyses were performed. Interestingly, evidence for the involvement of cold-induced, vernalization-related genes and repressors of endodormancy release was found. These genes have not previously been described to play a role in fruit during the ripening transition. The resulting data provide insight into cultivar-specific mechanisms of cold-induced transcriptional regulation of ripening in European pear, as well as a unique comparative analysis of the two cultivars with very different cold conditioning requirements.

ACS Style

Seanna Louise Hewitt; Christopher A. Hendrickson; Amit Dhingra. Evidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit. 2019, 851733 .

AMA Style

Seanna Louise Hewitt, Christopher A. Hendrickson, Amit Dhingra. Evidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit. . 2019; ():851733.

Chicago/Turabian Style

Seanna Louise Hewitt; Christopher A. Hendrickson; Amit Dhingra. 2019. "Evidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit." , no. : 851733.

Preprint
Published: 03 September 2019
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European pears (Pyrus communis L.) require a range of cold-temperature exposure to induce ethylene biosynthesis and fruit ripening. Physiological and hormonal responses to cold temperature storage in pear have been well characterized, but the molecular underpinnings of these phenomena remain unclear. An established low-temperature conditioning model was used to induce ripening of ‘D’Anjou’ and ‘Bartlett’ pear cultivars and quantify the expression of key genes representing ripening-related metabolic pathways in comparison to non-conditioned fruit. Physiological indicators of pear ripening were recorded, and fruit peel tissue sampled in parallel, during the cold-conditioning and ripening time-course experiment to correlate gene expression to ontogeny. Two complementary approaches, Nonparametric Multi-Dimensional Scaling and efficiency-corrected 2-(ΔΔCt), were used to identify genes exhibiting the most variability in expression. Interestingly, the enhanced alternative oxidase (AOX) transcript abundance at the pre-climacteric stage in ‘Bartlett’ and ‘D’Anjou’ at the peak of the conditioning treatments suggests that AOX may play a key and a novel role in the achievement of ripening competency. There were indications that cold-sensing and signaling elements from ABA and auxin pathways modulate the S1-S2 ethylene transition in European pears, and that the S1-S2 ethylene biosynthesis transition is more pronounced in ‘Bartlett’ as compared to ‘D’Anjou’ pear. This information has implications in preventing post-harvest losses of this important crop.

ACS Style

Christopher Hendrickson; SeAnna Hewitt; Mark E. Swanson; Todd Einhorn; Amit Dhingra; Christopher Hendrikckson. Evidence for pre-climacteric activation of AOX transcription during cold-induced conditioning to ripen in European pear (Pyrus communis L.). 2019, 755686 .

AMA Style

Christopher Hendrickson, SeAnna Hewitt, Mark E. Swanson, Todd Einhorn, Amit Dhingra, Christopher Hendrikckson. Evidence for pre-climacteric activation of AOX transcription during cold-induced conditioning to ripen in European pear (Pyrus communis L.). . 2019; ():755686.

Chicago/Turabian Style

Christopher Hendrickson; SeAnna Hewitt; Mark E. Swanson; Todd Einhorn; Amit Dhingra; Christopher Hendrikckson. 2019. "Evidence for pre-climacteric activation of AOX transcription during cold-induced conditioning to ripen in European pear (Pyrus communis L.)." , no. : 755686.

Preprint
Published: 03 September 2019
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Enhanced levels of antioxidants, phenolic compounds, carotenoids and vitamin C have been reported for several crops grown under organic fertilizer, albeit with yield penalties. As organic agricultural practices continue to grow and find favor it is critical to gain an understanding of the molecular underpinnings of the factors that limit the yields in organically farmed crops. Concomitant phytochemical and transcriptomic analysis was performed on mature fruit and leaf tissues derived from Solanum lycopersicum L. ‘Oregon Spring’ grown under organic and conventional fertilizer conditions to evaluate the following hypotheses. 1. Organic soil fertilizer management results in greater allocation of photosynthetically derived resources to the synthesis of secondary metabolites than to plant growth, and 2. Genes involved in changes in the accumulation of phytonutrients under organic fertilizer regime will exhibit differential expression, and that the growth under different fertilizer treatments will elicit a differential response from the tomato genome. Both these hypotheses were supported, suggesting an adjustment of the metabolic and genomic activity of the plant in response to different fertilizers. Organic fertilizer treatment showed an activation of photoinhibitory processes through differential activation of nitrogen transport and assimilation genes resulting in higher accumulation of phytonutrients. This information can be used to identify alleles for breeding crops that allow for efficient utilization of organic inputs.Significance statementOrganic fertilizer changes the expression of the tomato genome, induces photosynthetic stress which elicits higher production of secondary metabolites.

ACS Style

Richard M Sharpe; Luke Gustafson; SeAnna Hewitt; Benjamin Kilian; James Crabb; Christopher Hendrickson; Derick Jiwan; Preston Andrews; Amit Dhingra. Concomitant phytonutrient and transcriptome analysis of mature fruit and leaf tissues of tomato (Solanum lycopersicum L. cv. Oregon Spring) grown using organic and conventional fertilizer. 2019, 755769 .

AMA Style

Richard M Sharpe, Luke Gustafson, SeAnna Hewitt, Benjamin Kilian, James Crabb, Christopher Hendrickson, Derick Jiwan, Preston Andrews, Amit Dhingra. Concomitant phytonutrient and transcriptome analysis of mature fruit and leaf tissues of tomato (Solanum lycopersicum L. cv. Oregon Spring) grown using organic and conventional fertilizer. . 2019; ():755769.

Chicago/Turabian Style

Richard M Sharpe; Luke Gustafson; SeAnna Hewitt; Benjamin Kilian; James Crabb; Christopher Hendrickson; Derick Jiwan; Preston Andrews; Amit Dhingra. 2019. "Concomitant phytonutrient and transcriptome analysis of mature fruit and leaf tissues of tomato (Solanum lycopersicum L. cv. Oregon Spring) grown using organic and conventional fertilizer." , no. : 755769.

Research article
Published: 19 November 2017 in Agribusiness
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The North American fresh pear industry faces marketing challenges that could jeopardize its’ long‐term economic profitability. The production of sliced fresh pears is a promising alternative to overcome the lack of supplying consistently a product with superior quality with added convenience, potentially able to increase domestic consumption. In this paper, we used sensory evaluation and a Vickrey experimental auction to elicit consumers’ preferences and willingness to pay for sliced packed fresh pears treated with SmartFresh™ (1‐methylcyclopropene) and subsequently with a ripening compound (RC) in the form of glyoxylic acid at different concentration levels (1%, 2%, 3%, and control). Panelists were willing to pay a price premium equivalent to $0.119/2 oz packet for the 2% RC sample, $0.055/2 oz packet for the 3% RC sample, and $0.025/2 oz packet for the 1% RC sample compared to the control sample. Results from a market segmentation analyses indicate the presence of two groups in the panelist sample. The group that liked sliced pears assigned higher importance to locally grown fruit and price, shopped at conventional retailer grocery stores, had fewer children in the household, and were younger compared to the group that disliked sliced pears. [EconLit citation: Q13].

ACS Style

Dila Ikiz; R. Karina Gallardo; Amit Dhingra; SeAnna Hewitt. Assessing consumers’ preferences and willingness to pay for novel sliced packed fresh pears: A latent class approach. Agribusiness 2017, 34, 321 -337.

AMA Style

Dila Ikiz, R. Karina Gallardo, Amit Dhingra, SeAnna Hewitt. Assessing consumers’ preferences and willingness to pay for novel sliced packed fresh pears: A latent class approach. Agribusiness. 2017; 34 (2):321-337.

Chicago/Turabian Style

Dila Ikiz; R. Karina Gallardo; Amit Dhingra; SeAnna Hewitt. 2017. "Assessing consumers’ preferences and willingness to pay for novel sliced packed fresh pears: A latent class approach." Agribusiness 34, no. 2: 321-337.