In this study, a low-cost RGB sensor is developed to measure online the microalgae concentration within a photo-bioreactor. Two commercially available devices, i.e., a spectrophotometer for offline measurements and an immersed probe for online measurements, are used for calibration and comparison purposes. Furthermore, the potential of such a sensor for estimating other variables is illustrated with the design of an extended Luenberger observer.

The smart use of water is a key factor in increasing food production. Over the years, irrigation has relied on historical data and traditional management policies. Control techniques have been exploited to build automatic irrigation systems based on climatic records and weather forecasts. However, climate change and new sources of information motivate better irrigation strategies that might take advantage of the new sources of information in the spectrum of systems and control methodologies in a more systematic way. In this connection, two open questions deserve interest: (i) How can one deal with the space–time variability of soil conditions? (ii) How can one provide robustness to an irrigation system under unexpected environmental change? In this review, the different elements of an automatic control system are described, including the mathematical modeling of the crop–soil systems, instrumentation and actuation, model identification and validation from experimental data, estimation of non-measured variables and sensor fusion, and predictive control based on crop–soil and weather models. An overview of the literature is given, and several specific examples are worked out for illustration purposes.

Uncertainty is a common feature of biological systems, and model-free extremum-seeking control has proved a relevant approach to avoid the typical problems related to model-based optimization, e.g., time- and resource-consuming derivation and identification of dynamic models, and lack of robustness of optimal control. In this article, a review of the past and current trends in model-free extremum seeking is proposed with an emphasis on finding optimal operating conditions of bioprocesses. This review is illustrated with a simple simulation case study which allows a comparative evaluation of a few selected methods. Finally, some experimental case studies are discussed. As usual, practice lags behind theory, but recent developments confirm the applicability of the approach at the laboratory scale and are encouraging a transfer to industrial scale.