Fluctuations in solar radiation are one of the key factors affecting productivity and survival in habitat forming coastal macroalgae, in this regard, photoacclimation has a direct impact on the vulnerability and the capacity of seaweed to withstand, for instance, radiation excess. Here, we study ecophysiological responses through photosynthetic activity measurements under time-dependent (one year) fluctuations in solar radiation in the brown macroalga L. spicata. The responses presented seasonal patterns, with an increase in photosynthetic capacity during summer, expressed in greater maximal electron transport rate (ETR_max) and diminished thermal dissipation (NPQ_max). Moreover, we studied photoprotective compounds (phenolic compounds) and total antioxidant capacity, which demonstrated an increase during periods of high solar radiation. In addition, content of photosynthetic pigment (Chla, Chlc and Carotenoids) increased under greater solar irradiance. The L. spicata can accumulate as reservoir photoprotective and antioxidant substances to withstand periods of high solar irradiance. All ecophysiological and biochemical responses in L. spicata indicate high photoacclimation and low vulnerability in the species, especially during with greater levels of solar irradiance.

In this work, transcripts encoding three metallothioneins from Ulva compressa (UcMTs) were amplified: The 5′and 3′ UTRs by RACE-PCR, and the open reading frames (ORFs) by PCR. Transcripts encoding UcMT1.1 (Crassostrea-like), UcMT2 (Mytilus-like), and UcMT3 (Dreissena-like) showed a 5′UTR of 61, 71, and 65 nucleotides and a 3′UTR of 418, 235, and 193 nucleotides, respectively. UcMT1.1 ORF encodes a protein of 81 amino acids (MW 8.2 KDa) with 25 cysteines (29.4%), arranged as three motifs CC and nine motifs CXC; UcMT2 ORF encode a protein of 90 amino acids (9.05 kDa) with 27 cysteines (30%), arranged as three motifs CC, nine motifs CXC, and one motif CXXC; UcMT3 encode a protein of 139 amino acids (13.4 kDa) with 34 cysteines (24%), arranged as seven motifs CC and seven motifs CXC. UcMT1 and UcMT2 were more similar among each other, showing 60% similarity in amino acids; UcMT3 showed only 31% similarity with UcMT1 and UcMT2. In addition, UcMTs displayed structural similarity with MTs of marine invertebrates MTs and the terrestrial invertebrate Caenorhabtidis elegans MTs, but not with MTs from red or brown macroalgae. The ORFs fused with GST were expressed in bacteria allowing copper accumulation, mainly in MT1 and MT2, and zinc, in the case of the three MTs. Thus, the three MTs allowed copper and zinc accumulation in vivo. UcMTs may play a role in copper and zinc accumulation in U. compressa.