We evaluated immobilization stress and resveratrol supplementation in immature male mice at 30 days of age for 15 consecutive days. Fifty Swiss mice were divided into five groups (10 mice each): Controls, restraint stress (RS), restraint stress + vehicle (RS + V), RS + 2 mg/kg, and RS + 20 mg/kg. We determined results on the basis of hematoxylin and eosin (H&E), “Periodic acid-Schiff” staining, and TUNEL assay. The results indicated that immobilization stress significantly decreased body weight, testis weight, and water/food intake compared to the control; while resveratrol ameliorated these effects. The quantitative histologic evaluation of the seminiferous tubule diameter, luminal diameter, area of seminiferous tubules, area of tubule lumen, epithelial height, Leydig cell number, and the width of the tunica albuginea were similarly decreased after exposure to RS. These parameters recovered back to normal in the RS + 2 mg/kg group. The development of spermatogenesis was significantly delayed in the RS, RS + V, and RS + 20 mg groups based upon our evaluation score system. However, we observed no significant differences in the RS + 2 mg group compared with the control group. The number of TUNEL-positive cells also significantly decreased in the RS + 2 mg/kg group. In conclusion, we found that the administration of 2 mg/kg was an effective dose against immobilization stress in mice.

Our present study was designed to evaluate the effects of resveratrol (RES) in Swiss mice by exposing them to prenatal stress. Twenty-four Swiss mice were divided into four groups: control (C), maternal restraint stress (MRS), maternal restraint stress + resveratrol (MRS + RES) 2 mg, and maternal restraint stress + resveratrol (MRS + RES) 20 mg. Dams were exposed to stress by restraint in plastic tubes for four hours a day from 12–18 days of gestation. The results showed that male pups of MRS were significantly decreased in the testis weight, anogenital distance, area of seminiferous tubules, diameter of seminiferous tubules, area of the lumen, diameter of the lumen, and epithelial height of seminiferous tubules. However, the anomalies of the reproductive tract produced under restraint stress were neutralized by the use of RES 2 mg/kg. A significant difference was observed between terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)- positive germ cells in MRS and MRS + RES 20 mg/kg groups, while it was non-significant between MRS + RES 2 mg/kg and C groups. Apart from these effects, blood glucose levels were increased in MRS and MRS + RES 20 mg/kg groups, while experimental animals of the MRS + RES 2 mg/kg group significantly recovered. These results suggested that a lower dose of RES could cure the adverse effects of prenatal stress in early age male progeny. Thus, our study suggests, for the first time, practical values for a lower dose of RES 2 mg/kg as a safe and effective agent in the first week age of prenatally stressed mice.

The egg yolks play a significant role in preventing temperature related damages to the sperm’s acrosome and plasma membrane during cryopreservation. The present study aims to evaluate various egg yolk extenders in relation to quality indicators of post-thawed semen from Tapri goat bucks. Thus, total 60 ejaculates (n= 15/ animal) were collected from four healthy Tapri goat bucks entitled with A, B, C, and D. Semen samples were evaluated macro and microscopically and pooled in two groups viz; A and B. While preparing the Tris-based egg yolk extender; the egg yolk from duck was used in group “A” and Fayoumi chicken in group “B”. The diluted semen was packed in two different color straws (French straws) of 0.25 ml size, equilibrated for 2 hours at 5oC then frozen deep in the liquid nitrogen (at -196°C). The thawing of straws was attained at 37oC for 20 sec. The percentages of post-thawed total sperm motility, viability, normal morphology, and acrosomal membrane integrity were found significantly (p<0.05) better in group A (46.7±67, 49.33±1.397, 48.87±1.028, 50.60±1.302), then group B (42.67±0.6738, 43.73±0.9394, 44.73±1.112, 47±00), respectively. In conclusion, the extender containing duck egg yolk demonstrated significantly better (P<0.05) post-thaw quality parameters for Tapri goat bucks semen compared to that of fayoumi chicken.