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Simona Di Gregorio
Department of Biology, University of Pisa, 56122 Pisa, Italy

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Journal article
Published: 30 July 2021 in Sustainability
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This study performed a Life Cycle Assessment (LCA) on recovery strategies of dredged contaminated marine sediments in a large Mediterranean port located in central Italy (Tuscany) in order to find the most environmentally sound solution. The study considered marine sediments polluted by potentially toxic elements (PTEs) and/or organic compounds, two different sediment particle sizes and the combined use of three soil remediation technologies: soil washing, electrokinetic treatment and enhanced landfarming. The analyzed scenarios depended on the sediment properties and characteristics of the treatment technologies investigated, and were compared with the corresponding reference scenarios, consisting of the landfilling of dredged contaminated sediments. The LCA results show that scenarios associated with sediment recovery generated potential environmental impacts lower than the corresponding reference scenarios. Almost all the impact categories considered in the CML-IA baseline method showed an environmental convenience in the recovery of contaminated sediments, especially for abiotic depletion and global warming. Future studies should focus on optimizing the combined use of multiple technologies and reducing the resource consumptions related to their implementation in order to achieve both environmental and economic benefits.

ACS Style

Francesco Pasciucco; Isabella Pecorini; Simona Di Gregorio; Fabiano Pilato; Renato Iannelli. Recovery Strategies of Contaminated Marine Sediments: A Life Cycle Assessment. Sustainability 2021, 13, 8520 .

AMA Style

Francesco Pasciucco, Isabella Pecorini, Simona Di Gregorio, Fabiano Pilato, Renato Iannelli. Recovery Strategies of Contaminated Marine Sediments: A Life Cycle Assessment. Sustainability. 2021; 13 (15):8520.

Chicago/Turabian Style

Francesco Pasciucco; Isabella Pecorini; Simona Di Gregorio; Fabiano Pilato; Renato Iannelli. 2021. "Recovery Strategies of Contaminated Marine Sediments: A Life Cycle Assessment." Sustainability 13, no. 15: 8520.

Journal article
Published: 11 June 2021 in International Journal of Environmental Research and Public Health
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This study demonstrated that a microbial community dominated by fungi can be selected and maintained in the long-term under non-sterile conditions, in a pilot-scale packed-bed reactor fed with tannery wastewater. During the start-up phase, the reactor, filled with 0.6 m3 of polyurethane foam cubes, was inoculated with a pure culture of Aspergillus tubingensis and Quebracho tannin, a recalcitrant compound widely used by tannery industry, was used as sole carbon source in the feeding. During the start-up, fungi grew attached as biofilm in carriers that filled the packed-bed reactor. Subsequently, the reactor was tested for the removal of chemical oxygen demand (COD) from an exhaust tanning bath collected from tanneries. The entire experiment lasted 121 days and average removals of 29% and 23% of COD and dissolved organic carbon (DOC) from the tannins bath were achieved, respectively. The evolution of the microbial consortium (bacteria and fungi) was described through biomolecular analyses along the experiment and also developed as a function of the size of the support media.

ACS Style

Francesco Spennati; Salvatore La China; Giovanna Siracusa; Simona Di Gregorio; Alessandra Bardi; Valeria Tigini; Gualtiero Mori; David Gabriel; Giulio Munz. Tannery Wastewater Recalcitrant Compounds Foster the Selection of Fungi in Non-Sterile Conditions: A Pilot Scale Long-Term Test. International Journal of Environmental Research and Public Health 2021, 18, 6348 .

AMA Style

Francesco Spennati, Salvatore La China, Giovanna Siracusa, Simona Di Gregorio, Alessandra Bardi, Valeria Tigini, Gualtiero Mori, David Gabriel, Giulio Munz. Tannery Wastewater Recalcitrant Compounds Foster the Selection of Fungi in Non-Sterile Conditions: A Pilot Scale Long-Term Test. International Journal of Environmental Research and Public Health. 2021; 18 (12):6348.

Chicago/Turabian Style

Francesco Spennati; Salvatore La China; Giovanna Siracusa; Simona Di Gregorio; Alessandra Bardi; Valeria Tigini; Gualtiero Mori; David Gabriel; Giulio Munz. 2021. "Tannery Wastewater Recalcitrant Compounds Foster the Selection of Fungi in Non-Sterile Conditions: A Pilot Scale Long-Term Test." International Journal of Environmental Research and Public Health 18, no. 12: 6348.

Microbiology
Published: 08 June 2021 in Frontiers in Microbiology
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A Ciboria sp. strain (Phylum Ascomycota) was isolated from hydrocarbon-polluted soil of an abandoned oil refinery in Italy. The strain was able to utilize diesel oil as a sole carbon source for growth. Laboratory-scale experiments were designed to evaluate the use of this fungal strain for treatment of the polluted soil. The concentration of total petroleum hydrocarbons (TPH) in the soil was 8,538 mg/kg. Mesocosms containing the contaminated soil were inoculated with the fungal strain at 1 or 7%, on a fresh weight base ratio. After 90 days of incubation, the depletion of TPH contamination was of 78% with the 1% inoculant, and 99% with the 7% inoculant. 16S rDNA and ITS metabarcoding of the bacterial and fungal communities was performed in order to evaluate the potential synergism between fungi and bacteria in the bioremediation process. The functional metagenomic prediction indicated Arthrobacter, Dietzia, Brachybacerium, Brevibacterium, Gordonia, Leucobacter, Lysobacter, and Agrobacterium spp. as generalist saprophytes, essential for the onset of hydrocarbonoclastic specialist bacterial species, identified as Streptomyces, Nocardoides, Pseudonocardia, Solirubrobacter, Parvibaculum, Rhodanobacter, Luteiomonas, Planomicrobium, and Bacillus spp., involved in the TPH depletion. The fungal metabolism accelerated the onset of specialist over generalist bacteria. The capacity of the Ciboria sp. to deplete TPH in the soil in treatment was also ascertained.

ACS Style

Simone Becarelli; Ilaria Chicca; Salvatore La China; Giovanna Siracusa; Alessandra Bardi; Maria Gullo; Giulio Petroni; David Bernard Levin; Simona Di Gregorio. A New Ciboria sp. for Soil Mycoremediation and the Bacterial Contribution to the Depletion of Total Petroleum Hydrocarbons. Frontiers in Microbiology 2021, 12, 1 .

AMA Style

Simone Becarelli, Ilaria Chicca, Salvatore La China, Giovanna Siracusa, Alessandra Bardi, Maria Gullo, Giulio Petroni, David Bernard Levin, Simona Di Gregorio. A New Ciboria sp. for Soil Mycoremediation and the Bacterial Contribution to the Depletion of Total Petroleum Hydrocarbons. Frontiers in Microbiology. 2021; 12 ():1.

Chicago/Turabian Style

Simone Becarelli; Ilaria Chicca; Salvatore La China; Giovanna Siracusa; Alessandra Bardi; Maria Gullo; Giulio Petroni; David Bernard Levin; Simona Di Gregorio. 2021. "A New Ciboria sp. for Soil Mycoremediation and the Bacterial Contribution to the Depletion of Total Petroleum Hydrocarbons." Frontiers in Microbiology 12, no. : 1.

Research article
Published: 18 June 2020 in Environmental Science and Pollution Research
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A new Pseudomonas putida strain (AQ8) was isolated from a decommissioned oil refinery’s soil in Italy and characterized for its ability to degrade BTEX. The draft genome of the new strain was sequenced and annotated for genes that encode enzymes putatively involved in BTEX degradation and quorum sensing. The strain was transformed with a plasmid expressing lactonase, which cleaves the autoinducer quorum sensing signal molecule, the acyl-homoserine lactone, to obtain a quorum sensing minus strain. P. putida AQ8 depleted the 40% on average of all the components of the initial BTEX concentration in 36 h. The quorum sensing minus strain, in the same time interval, depleted only the 10% of the initial BTEX concentration. The role of quorum sensing in regulating the expression of the annotated benzene/toluene dioxygenase gene (benzA) and biphenyl/toluene/benzene dioxygenase (bphA) genes, which are involved in BTEX degradation, was studied by quantitative RT-real-time quantitative (q)PCR analysis. The qPCR data showed decreased levels of expression of the benzA and bphA genes in the quorum sensing minus strain. Our results showed, for the first time, quorum sensing modulation of the level of transcription of dioxygenase genes in the upper BTEX oxidation pathway.

ACS Style

Ilaria Chicca; Simone Becarelli; Christopher Dartiahl; Salvatore La China; Teresa De Kievit; Giulio Petroni; Simona Di Gregorio; David B. Levin. Degradation of BTEX mixture by a new Pseudomonas putida strain: role of the quorum sensing in the modulation of the upper BTEX oxidative pathway. Environmental Science and Pollution Research 2020, 27, 36203 -36214.

AMA Style

Ilaria Chicca, Simone Becarelli, Christopher Dartiahl, Salvatore La China, Teresa De Kievit, Giulio Petroni, Simona Di Gregorio, David B. Levin. Degradation of BTEX mixture by a new Pseudomonas putida strain: role of the quorum sensing in the modulation of the upper BTEX oxidative pathway. Environmental Science and Pollution Research. 2020; 27 (29):36203-36214.

Chicago/Turabian Style

Ilaria Chicca; Simone Becarelli; Christopher Dartiahl; Salvatore La China; Teresa De Kievit; Giulio Petroni; Simona Di Gregorio; David B. Levin. 2020. "Degradation of BTEX mixture by a new Pseudomonas putida strain: role of the quorum sensing in the modulation of the upper BTEX oxidative pathway." Environmental Science and Pollution Research 27, no. 29: 36203-36214.

Journal article
Published: 13 May 2020 in Water
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A new halo-alkaline sulfur-oxidising bacterial strain was isolated from brackish estuary sediments contaminated by total petroleum hydrocarbon. The isolate was classified as a new strain of Thioalkalivibrio sulfidiphilus sp., showing a higher capability of adaptation to pH and a higher optimal sodium concentration for growth, when compared to Thioalkalivibrio sulfidiphilus sp. HL-EbGr7, type strain of the species. The strain was capable to grow in saline concentrations up to 1.5 M Na+ and pH up to 10. The genome of the new isolate was sequenced and annotated. The comparison with the genome of Thioalkalivibrio sulfidiphilus sp. HL-EbGr7 showed a duplication of an operon encoding for a putative primary sodium extruding pump and the presence of a sodium/proton antiporter with optimal efficiency at halo-alkaline conditions. The new strain was able to oxidize sulfide at halo-alkaline conditions at the rate of 1 mmol/mg-N/h, suitable for industrial applications dedicated to the recovery of alkaline scrubber for H2S emission absorption and abatement.

ACS Style

Simone Becarelli; Salvatore La China; Alla Lapidus; Andrey Prijibelski; Dmitrii Polev; Giulio Petroni; Simona Di Gregorio. A New Thioalkalivibrio sp. Strain Isolated from Petroleum-Contaminated Brackish Estuary Sediments: A New Candidate for Bio-Based Application for Sulfide Oxidation in Halo-Alkaline Conditions. Water 2020, 12, 1385 .

AMA Style

Simone Becarelli, Salvatore La China, Alla Lapidus, Andrey Prijibelski, Dmitrii Polev, Giulio Petroni, Simona Di Gregorio. A New Thioalkalivibrio sp. Strain Isolated from Petroleum-Contaminated Brackish Estuary Sediments: A New Candidate for Bio-Based Application for Sulfide Oxidation in Halo-Alkaline Conditions. Water. 2020; 12 (5):1385.

Chicago/Turabian Style

Simone Becarelli; Salvatore La China; Alla Lapidus; Andrey Prijibelski; Dmitrii Polev; Giulio Petroni; Simona Di Gregorio. 2020. "A New Thioalkalivibrio sp. Strain Isolated from Petroleum-Contaminated Brackish Estuary Sediments: A New Candidate for Bio-Based Application for Sulfide Oxidation in Halo-Alkaline Conditions." Water 12, no. 5: 1385.

Articles
Published: 15 April 2020 in Environmental Technology
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A two-stage biological process using fungi and bacteria was set-up and tested for treating a petrochemical wastewater containing naphthalene sulphonic acid polymers. The fungal treatment was carried out through a trickling filter inoculated with Pleurotus ostreatus attached on Luffa cylindrica acting as both physical support and carbon source. The fungal reactor was operated in non-sterile conditions setting two pH values (5 and 6) and two hydraulic retention times (2 d and 3 d). The effluent was then sent to an activated sludge reactor operating the second stage of the treatment. Using an HPLC-based technique, it was observed that the fungal reactor was capable of reducing the polymerization grade of naphthalene sulphonic acid polymers up to 35%, thus increasing significantly the biodegradability of the petrochemical wastewater, from the initial 9% to 46%. The two-stage process allowed to remove about 50% of the total COD much higher than 9% that can be achieved with activated sludge alone. The use of Luffa cylindrica as support for fungi allowed to limit bacterial contamination of the trickling filter and enhanced enzymatic production (on average 20 U/L of Laccase) without any release of non-biodegradable by-products in the effluent. Extraction and PCR-amplification of fungal DNA was carried out along with over 70 d running process in order to monitor the changes of the fungal community inside the reactors. Results showed that Meyerozyma, Fusarium and Thricoderma, spp. developed inside the reactor with Thricoderma, spp. representing the main constituent of fungal biomass at the end of the experiment.

ACS Style

Laura Palli; Silvia Tilli; Simona Di Gregorio; Andrea Scozzafava; Riccardo Gori. Treatment of NSAPs-rich petrochemical wastewaters using a two-stage combined process of fungi and activated sludge. Environmental Technology 2020, 1 -14.

AMA Style

Laura Palli, Silvia Tilli, Simona Di Gregorio, Andrea Scozzafava, Riccardo Gori. Treatment of NSAPs-rich petrochemical wastewaters using a two-stage combined process of fungi and activated sludge. Environmental Technology. 2020; ():1-14.

Chicago/Turabian Style

Laura Palli; Silvia Tilli; Simona Di Gregorio; Andrea Scozzafava; Riccardo Gori. 2020. "Treatment of NSAPs-rich petrochemical wastewaters using a two-stage combined process of fungi and activated sludge." Environmental Technology , no. : 1-14.

Journal article
Published: 13 March 2020 in Water
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In the present study, an Ascomycete fungal strain, Lambertella sp., isolated from environmental polluted matrices, was tested for the capacity to reduce the contamination and the toxicity of intermediate and old landfill leachates. Batch tests in flasks, under co-metabolic conditions, were performed with two different old leachates, with suspended and immobilized Lambertella sp. biomass, resulting in a soluble chemical oxygen demand depletion of 70% and 45%, after 13 and 30 days, respectively. An intermediate landfill leachate was treated in lab-scale reactors operating in continuous conditions for three months, inoculated with immobilized Lambertella sp. biomass, in absence of co-substrates. The Lambertella sp. depleted the corresponding total organic carbon by 90.2%. The exploitability of the Lambertella sp. strain was evaluated also in terms of reduction of phyto-, cyto-, and mutagenicity of the different Landfill Leachates at the end of the myco-based treatment, resulting in an efficient depletion of leachate clastogenicity.

ACS Style

Giovanna Siracusa; Qiuyan Yuan; Ilaria Chicca; Alessandra Bardi; Francesco Spennati; Simone Becarelli; David Bernard Levin; Giulio Munz; Giulio Petroni; Simona Di Gregorio. Mycoremediation of Old and Intermediate Landfill Leachates with an Ascomycete Fungal Isolate, Lambertella sp. Water 2020, 12, 800 .

AMA Style

Giovanna Siracusa, Qiuyan Yuan, Ilaria Chicca, Alessandra Bardi, Francesco Spennati, Simone Becarelli, David Bernard Levin, Giulio Munz, Giulio Petroni, Simona Di Gregorio. Mycoremediation of Old and Intermediate Landfill Leachates with an Ascomycete Fungal Isolate, Lambertella sp. Water. 2020; 12 (3):800.

Chicago/Turabian Style

Giovanna Siracusa; Qiuyan Yuan; Ilaria Chicca; Alessandra Bardi; Francesco Spennati; Simone Becarelli; David Bernard Levin; Giulio Munz; Giulio Petroni; Simona Di Gregorio. 2020. "Mycoremediation of Old and Intermediate Landfill Leachates with an Ascomycete Fungal Isolate, Lambertella sp." Water 12, no. 3: 800.

Journal article
Published: 21 June 2019 in Journal of Environmental Management
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A number of bacteria and fungi are known to degrade tannins. In this study, the efficiency of the white-rot fungus, Bjerkandera adusta MUT 2295, was evaluated for the treatment of a synthetic solution prepared with tannic acid. Tests were performed in continuously fed, bench-scale, packed-bed reactors, operated under non-sterile conditions with biomass immobilized within PolyUrethane Foam cubes (PUFs). The main parameters monitored to evaluate the process efficiency were: soluble Chemical Oxygen Demand (sCOD), Total Organic Carbon (TOC) removal, and activities. of Tannase and Lignin Peroxidase. At the end of the process, additional parameters were evaluated, including the increase of fungal dry weight and the presence of ergosterol. The reactor was operative for 210 days, with maximum sCOD and TOC removal of 81% and 73%, respectively. The reduction of sCOD and TOC were positively correlated with the detection of Tannase and Lignin Peroxidase (LiP) activities. Increases in biomass within the PUF cubes was associated with increases in ergosterol concentrations. This study proved that the fungal-based system tested was efficient for the degradation of tannic acid over a period of time, and under non-sterile conditions.

ACS Style

A. Bardi; Q. Yuan; G. Siracusa; S. Becarelli; S. Di Gregorio; V. Tigini; D.B. Levin; G. Petroni; Giulio Munz. Stability of fungal biomass continuously fed with tannic acid in a non-sterile moving-packed bed reactor. Journal of Environmental Management 2019, 247, 67 -77.

AMA Style

A. Bardi, Q. Yuan, G. Siracusa, S. Becarelli, S. Di Gregorio, V. Tigini, D.B. Levin, G. Petroni, Giulio Munz. Stability of fungal biomass continuously fed with tannic acid in a non-sterile moving-packed bed reactor. Journal of Environmental Management. 2019; 247 ():67-77.

Chicago/Turabian Style

A. Bardi; Q. Yuan; G. Siracusa; S. Becarelli; S. Di Gregorio; V. Tigini; D.B. Levin; G. Petroni; Giulio Munz. 2019. "Stability of fungal biomass continuously fed with tannic acid in a non-sterile moving-packed bed reactor." Journal of Environmental Management 247, no. : 67-77.

Articles
Published: 14 May 2019 in Environmental Technology
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Tannins are polyphenolic compounds produced by plants and they are used in industrial vegetable tanning of leather. Tannins represent one of the low biodegradability substances in tannery wastewaters with high recalcitrant soluble chemical oxygen demand, furthermore high concentration of tannins can inhibit biological treatment. In the present study four novel rotating submerged packed bed reactors were inoculated with a selected fungal strain to reach a biological degradation of tannins in non-sterile conditions. The selected fungal strain, Aspergillus tubingensis MUT 990, was immobilized in polyurethane foam cubes carriers and inserted inside a submerged rotating cage reactors. The reactors were feed with a solution composed by four tannins: Quebracho (Schinopsis spp.), Wattle (Mimosa spp.), Chestnut (Castanea spp.) and Tara (Cæsalpinia spp.). Four reactors with a volume of 4 L each were used, the co-substrate was pure malt extract, the hydraulic retention time was 24 hours and the pH setpoint was 5.5. The reactors configuration was chosen to allow the study of the effect of rotation and the co-substrate addition on tannins removal. The experiment lasted two months and it was achieved 80% of chemical oxygen demand and up to 90% dissolved organic carbon removal, furthermore it was detected an important tannase activity.

ACS Style

Francesco Spennati; Andrea Ricotti; Gualtiero Mori; Giovanna Siracusa; Simone Becarelli; Simona Di Gregorio; Valeria Tigini; Giovanna Cristina Varese; Giulio Munz. The role of cosubstrate and mixing on fungal biofilm efficiency in the removal of tannins. Environmental Technology 2019, 41, 3515 -3523.

AMA Style

Francesco Spennati, Andrea Ricotti, Gualtiero Mori, Giovanna Siracusa, Simone Becarelli, Simona Di Gregorio, Valeria Tigini, Giovanna Cristina Varese, Giulio Munz. The role of cosubstrate and mixing on fungal biofilm efficiency in the removal of tannins. Environmental Technology. 2019; 41 (26):3515-3523.

Chicago/Turabian Style

Francesco Spennati; Andrea Ricotti; Gualtiero Mori; Giovanna Siracusa; Simone Becarelli; Simona Di Gregorio; Valeria Tigini; Giovanna Cristina Varese; Giulio Munz. 2019. "The role of cosubstrate and mixing on fungal biofilm efficiency in the removal of tannins." Environmental Technology 41, no. 26: 3515-3523.

Journal article
Published: 14 January 2019 in New Biotechnology
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Four new Ascomycete fungi capable of degrading diesel oil were isolated from sediments of a river estuary mainly contaminated by shipyard fuels or diesel oil. The isolates were identified as species of Lambertella, Penicillium, Clonostachys, and Mucor. The fungal candidates degraded and adsorbed the diesel oil in suspension cultures. The Lambertella sp. isolate displayed the highest percentages of oxidation of diesel oil and was characterised by the capacity to utilise the latter as a sole carbon source. This isolate showed extracellular laccase and Mn-peroxidase activities in the presence of diesel oil. It was tested for capacity to accelerate the process of decontamination of total petroleum hydrocarbon contaminated sediments, co-composted with lignocellulosic residues and was able to promote the degradation of 47.6% of the TPH contamination (54,074 ± 321 mg TPH/Kg of sediment) after two months of incubation. The response of the bacterial community during the degradation process was analysed by 16S rRNA gene meta-barcoding.

ACS Style

Simone Becarelli; Ilaria Chicca; Giovanna Siracusa; Salvatore La China; Alessandro Gentini; Roberto Lorenzi; Giulio Munz; Giulio Petroni; David B. Levin; Simona Di Gregorio. Hydrocarbonoclastic Ascomycetes to enhance co-composting of total petroleum hydrocarbon (TPH) contaminated dredged sediments and lignocellulosic matrices. New Biotechnology 2019, 50, 27 -36.

AMA Style

Simone Becarelli, Ilaria Chicca, Giovanna Siracusa, Salvatore La China, Alessandro Gentini, Roberto Lorenzi, Giulio Munz, Giulio Petroni, David B. Levin, Simona Di Gregorio. Hydrocarbonoclastic Ascomycetes to enhance co-composting of total petroleum hydrocarbon (TPH) contaminated dredged sediments and lignocellulosic matrices. New Biotechnology. 2019; 50 ():27-36.

Chicago/Turabian Style

Simone Becarelli; Ilaria Chicca; Giovanna Siracusa; Salvatore La China; Alessandro Gentini; Roberto Lorenzi; Giulio Munz; Giulio Petroni; David B. Levin; Simona Di Gregorio. 2019. "Hydrocarbonoclastic Ascomycetes to enhance co-composting of total petroleum hydrocarbon (TPH) contaminated dredged sediments and lignocellulosic matrices." New Biotechnology 50, no. : 27-36.

Research article
Published: 29 October 2018 in Environmental Science and Pollution Research
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This study assessed the ability to remove micro-pollutants from wastewater using herbaceous species (Phragmites australis L.) and trees (Salix matsudana Koidz.) in constructed wetland (CW) systems. The targets of the study were as follows: (i) pharmaceuticals like diclofenac, ketoprofen, and atenolol; (ii) 4-n-NP (4-n-nonylphenol) and the ethoxylated derivatives monoethoxylated nonylphenol (NP1EO) and diethoxylated nonylphenol (NP2EO); (iii) triclosan, a bactericide used in personal care products. The 12 CW systems, filled with clay and gravel, were irrigated with wastewater from municipal area of Pagnana (Tuscany, Italy) and influent and effluent water samples analyzed periodically by gas chromatography-mass spectrometry (GC-MS/MS). The removal efficiency of CWs planted with willow and common red ranged from 8.4 up to 100%, with the higher removal efficiency for triclosan. On the contrary, the removal efficiency of NPs and NPEOs appears lower than pharmaceuticals. Data demonstrated that P. australis efficiently removed NP, diclofenac, and atenolol, while S. matsudana preferentially removed NP1EO, NP2EO, ketoprofene, and triclosan. A specific selection of plants used in CWs could be exploited for the removal of specific xenobiotics from wastewater.

ACS Style

Alessandra Francini; Lorenzo Mariotti; Simona Di Gregorio; Luca Sebastiani; Andrea Andreucci. Removal of micro-pollutants from urban wastewater by constructed wetlands with Phragmites australis and Salix matsudana. Environmental Science and Pollution Research 2018, 25, 36474 -36484.

AMA Style

Alessandra Francini, Lorenzo Mariotti, Simona Di Gregorio, Luca Sebastiani, Andrea Andreucci. Removal of micro-pollutants from urban wastewater by constructed wetlands with Phragmites australis and Salix matsudana. Environmental Science and Pollution Research. 2018; 25 (36):36474-36484.

Chicago/Turabian Style

Alessandra Francini; Lorenzo Mariotti; Simona Di Gregorio; Luca Sebastiani; Andrea Andreucci. 2018. "Removal of micro-pollutants from urban wastewater by constructed wetlands with Phragmites australis and Salix matsudana." Environmental Science and Pollution Research 25, no. 36: 36474-36484.

Journal article
Published: 16 October 2018 in Journal of Environmental Management
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Tannins are polyphenolic compounds produced by plants that are used in the vegetable tanning of leather at industrial scale. Quebracho tannin and Tara tannin are intensively used by the tanning industry and are two of the most recalcitrant compounds that can be found in tannery wastewaters. In this study two reactors fed with Quebracho tannin and Tara tannin, respectively, were inoculated with polyurethane foam cubes colonized with a fungal strain biofilm of Aspergillus tubingensis MUT 990. A stable biofilm was maintained in the reactor fed with Quebracho tannin during 180 days of operation. Instead, biofilm got detached from the foam cubes during the start-up of the reactor fed with Tara tannin and a bacterial-based suspended culture was developed and preserved along the operational period (226 days). Soluble chemical oxygen demand removals up to 53% and 90% and maximum elimination capacities of 9.1 g sCOD m−3 h−1 and 37.9 g sCOD m−3 h−1 of Quebracho and Tara tannins, respectively, were achieved in the reactors without the addition of co-substrates. Next generation sequencing analysis for bacteria and fungi showed that a fungal consortium was developed in the reactor fed with Quebracho tannin while fungi were outcompeted by bacteria in the reactor fed with Tara tannin. Furthermore, Quebracho and Tara tannins were successfully co-treated in a single reactor where both fungi and bacteria were preserved.

ACS Style

F. Spennati; M. Mora; V. Tigini; Salvatore La China; S. Di Gregorio; David Gabriel; Giulio Munz. Removal of Quebracho and Tara tannins in fungal bioreactors: Performance and biofilm stability analysis. Journal of Environmental Management 2018, 231, 137 -145.

AMA Style

F. Spennati, M. Mora, V. Tigini, Salvatore La China, S. Di Gregorio, David Gabriel, Giulio Munz. Removal of Quebracho and Tara tannins in fungal bioreactors: Performance and biofilm stability analysis. Journal of Environmental Management. 2018; 231 ():137-145.

Chicago/Turabian Style

F. Spennati; M. Mora; V. Tigini; Salvatore La China; S. Di Gregorio; David Gabriel; Giulio Munz. 2018. "Removal of Quebracho and Tara tannins in fungal bioreactors: Performance and biofilm stability analysis." Journal of Environmental Management 231, no. : 137-145.

Conference report
Published: 27 October 2017 in Water
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Recalcitrant compounds limit the efficiency of conventional biological processes for wastewater treatment, representing one of the major issues in the field. This study focused on the treatment of three effluents with White-Rot-Fungus (WRF) Bjerkandera adusta MUT 2295 in batch tests, with biomass cultivated in attached form on polyurethane foam cubes (PUFs) to test its efficiency in the removal of the target effluents’ recalcitrant fraction. Treatment efficiency of B. adusta was evaluated on landfill leachate (Canada) and two solutions containing synthetic recalcitrant compounds, which were prepared with tannic and humic acid. Chemical Oxygen Demand (COD) and color removal, the production of manganese peroxidases, and the consumption of a co-substrate (glucose) were monitored during the experiment. Biological Oxygen Demand (BOD5) and fungal dry weight were measured at the beginning and at the end of the experiment. After co-substrate addition, effluent COD was 2300 ± 85, 2545 ± 84, and 2580 ± 95 (mg/L) in raw leachate and tannic and humic acids, respectively. COD removal of 48%, 61%, and 48% was obtained in raw leachate and in the synthetic effluents containing tannic and humic acids, respectively. Color removal of 49%, 25%, and 42% was detected in raw leachate and in tannic and humic acid solutions, respectively. COD and color removals were associated with the increase of fungal dry weight, which was observed in all the trials. These results encourage the use of the selected fungal strain to remove tannic acid, while further investigations are required to optimize leachate and humic acid bioremediation.

ACS Style

Alessandra Bardi; Qiuyan Yuan; Valeria Tigini; Federica Spina; Giovanna Cristina Varese; Francesco Spennati; Simone Becarelli; Simona Di Gregorio; Giulio Petroni; Giulio Munz. Recalcitrant Compounds Removal in Raw Leachate and Synthetic Effluents Using the White-Rot Fungus Bjerkandera adusta. Water 2017, 9, 824 .

AMA Style

Alessandra Bardi, Qiuyan Yuan, Valeria Tigini, Federica Spina, Giovanna Cristina Varese, Francesco Spennati, Simone Becarelli, Simona Di Gregorio, Giulio Petroni, Giulio Munz. Recalcitrant Compounds Removal in Raw Leachate and Synthetic Effluents Using the White-Rot Fungus Bjerkandera adusta. Water. 2017; 9 (11):824.

Chicago/Turabian Style

Alessandra Bardi; Qiuyan Yuan; Valeria Tigini; Federica Spina; Giovanna Cristina Varese; Francesco Spennati; Simone Becarelli; Simona Di Gregorio; Giulio Petroni; Giulio Munz. 2017. "Recalcitrant Compounds Removal in Raw Leachate and Synthetic Effluents Using the White-Rot Fungus Bjerkandera adusta." Water 9, no. 11: 824.

Journal article
Published: 01 October 2017 in New Biotechnology
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Polychlorinated biphenyls (PCBs) are hazardous soil contaminants for which a bio-based technology for their recovery is essential. The objective of this study was to validate the exploitation of spent mushroom substrate (SMS), a low or null cost organic waste derived from the industrial production of P. ostreatus, as bulking agent in a dynamic biopile pilot plant. The SMS shows potential oxidative capacity towards recalcitrant compounds. The aim was consistent with the design of a process of oxidation of highly chlorinated PCBs, which is independent from their reductive dehalogenation. Feasibility was verified at a mesocosm scale and validated at pilot scale in a dynamic biopile pilot plant treating ten tons of a historically contaminated soil (9.28±0.08mg PCB/kg soil dry weight). Mixing of the SMS with the soil was required for the depletion of the contaminants. At the pilot scale, after eight months of incubation, 94.1% depletion was recorded. A positive correlation between Actinobacteria and Firmicutes active metabolism, soil laccase activity and PCB removal was observed. The SMS was found to be exploitable as a versatile low cost organic substrate capable of activating processes for the oxidation of highly chlorinated PCBs. Moreover, its exploitation as bulking agent in biopiles is a valuable management strategy for the re-utilisation of an organic waste deriving from the industrial cultivation of edible mushrooms.

ACS Style

Giovanna Siracusa; Simone Becarelli; Roberto Lorenzi; Alessandro Gentini; Simona Di Gregorio. PCB in the environment: bio-based processes for soil decontamination and management of waste from the industrial production of Pleurotus ostreatus. New Biotechnology 2017, 39, 232 -239.

AMA Style

Giovanna Siracusa, Simone Becarelli, Roberto Lorenzi, Alessandro Gentini, Simona Di Gregorio. PCB in the environment: bio-based processes for soil decontamination and management of waste from the industrial production of Pleurotus ostreatus. New Biotechnology. 2017; 39 ():232-239.

Chicago/Turabian Style

Giovanna Siracusa; Simone Becarelli; Roberto Lorenzi; Alessandro Gentini; Simona Di Gregorio. 2017. "PCB in the environment: bio-based processes for soil decontamination and management of waste from the industrial production of Pleurotus ostreatus." New Biotechnology 39, no. : 232-239.

Journal article
Published: 01 July 2016 in New Biotechnology
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Simona Di Gregorio; Paolo Maggioni; Roberto Lorenzi. Bioaugmentation of an allochtonous Ascomycetes for the depletion of the polychlorinated biphenyls (PCBs) in contaminated soils. New Biotechnology 2016, 33, S137 .

AMA Style

Simona Di Gregorio, Paolo Maggioni, Roberto Lorenzi. Bioaugmentation of an allochtonous Ascomycetes for the depletion of the polychlorinated biphenyls (PCBs) in contaminated soils. New Biotechnology. 2016; 33 ():S137.

Chicago/Turabian Style

Simona Di Gregorio; Paolo Maggioni; Roberto Lorenzi. 2016. "Bioaugmentation of an allochtonous Ascomycetes for the depletion of the polychlorinated biphenyls (PCBs) in contaminated soils." New Biotechnology 33, no. : S137.

Journal article
Published: 01 July 2016 in New Biotechnology
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ACS Style

Simona Di Gregorio; Alessandro Gentini; Gualtiero Masini; Giovanna Siracusa; Simone Becarelli; Roberto Lorenzi. Bioresnova project: the recovery of dredged sediments contaminated by total petroleum hydrocarbon to productive soils. New Biotechnology 2016, 33, S31 .

AMA Style

Simona Di Gregorio, Alessandro Gentini, Gualtiero Masini, Giovanna Siracusa, Simone Becarelli, Roberto Lorenzi. Bioresnova project: the recovery of dredged sediments contaminated by total petroleum hydrocarbon to productive soils. New Biotechnology. 2016; 33 ():S31.

Chicago/Turabian Style

Simona Di Gregorio; Alessandro Gentini; Gualtiero Masini; Giovanna Siracusa; Simone Becarelli; Roberto Lorenzi. 2016. "Bioresnova project: the recovery of dredged sediments contaminated by total petroleum hydrocarbon to productive soils." New Biotechnology 33, no. : S31.

Journal article
Published: 29 March 2016 in Journal of Chemical Technology & Biotechnology
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Polycyclic aromatic hydrocarbons (PAHs) are hazardous contaminants. Bio-based technology are among recommended practices for the recovery of PAH contaminated matrices. The objective of the study was to validate the exploitation of the spent mushroom substrate (SMS), an organic waste deriving from the industrial production of P. ostreatus, as bulking agent in a dynamic biopile pilot plant, because of the SMS potential oxidative capacity towards aromatic recalcitrant compounds. The dynamic biopile pilot plant treated seven tons of a historically PAH contaminated soil (6469 ±423 mg PAHs/Kg), classified as dangerous waste. The mixing of SMS with soil resulted to be mandatory for the depletion of PAHs that, after eight months, were at the significantly lower concentration (112 ± 5 mg PAHs/Kg). For the legislative context, the treated soil was compatible with the reintroduction in the industrial site of origin. However, a residual genotoxicity of soil elutriates at the end of the process was measured on root tips of Vicia faba L.. The SMS deriving from the industrial production of P. ostreatus is exploitable as a versatile low cost organic substrate with oxidative capacity towards PAHs and its exploitation as bulking agent in biopiles is advantageous for the disposal of the organic waste.

ACS Style

Simona Di Gregorio; Simone Becarelli; Giovanna Siracusa; Monica Ruffini Castiglione; Giulio Petroni; Gualtiero Masini; Alessandro Gentini; Mara Silva; Roberto Lorenzi. Pleurotus ostreatusspent mushroom substrate for the degradation of polycyclic aromatic hydrocarbons: the case study of a pilot dynamic biopile for the decontamination of a historically contaminated soil. Journal of Chemical Technology & Biotechnology 2016, 91, 1654 -1664.

AMA Style

Simona Di Gregorio, Simone Becarelli, Giovanna Siracusa, Monica Ruffini Castiglione, Giulio Petroni, Gualtiero Masini, Alessandro Gentini, Mara Silva, Roberto Lorenzi. Pleurotus ostreatusspent mushroom substrate for the degradation of polycyclic aromatic hydrocarbons: the case study of a pilot dynamic biopile for the decontamination of a historically contaminated soil. Journal of Chemical Technology & Biotechnology. 2016; 91 (6):1654-1664.

Chicago/Turabian Style

Simona Di Gregorio; Simone Becarelli; Giovanna Siracusa; Monica Ruffini Castiglione; Giulio Petroni; Gualtiero Masini; Alessandro Gentini; Mara Silva; Roberto Lorenzi. 2016. "Pleurotus ostreatusspent mushroom substrate for the degradation of polycyclic aromatic hydrocarbons: the case study of a pilot dynamic biopile for the decontamination of a historically contaminated soil." Journal of Chemical Technology & Biotechnology 91, no. 6: 1654-1664.

Journal article
Published: 15 January 2016 in Environmental Science and Pollution Research
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Two bacterial strains, Achromobacter sp. (ACH01) and Sphingomonas sp. (SPH01), were isolated from a heavily polycyclic aromatic hydrocarbon (PAH)-contaminated soil (5431.3 ± 102.3 ppm) for their capacity to use a mixture of anthracene, pyrene, phenanthrene and fluorene as sole carbon sources for growth and for the capacity to produce biosurfactants. The two strains were exploited for bioaugmentation in a biopile pilot plant to increase the bioavailability and the degradation of the residual PAH contamination (99.5 ± 7.1 ppm) reached after 9 months of treatment. The denaturing gel gradient electrophoresis (DGGE) profile of the microbial ecology of the soil during the experimentation showed that the bioaugmentation approach was successful in terms of permanence of the two strains in the soil in treatment. The bioaugmentation of the two bacterial isolates positively correlated with the PAH depletion that reached 7.9 ± 2 ppm value in 2 months of treatment. The PAH depletion was assessed by the loss of the phyto-genotoxicity of soil elutriates on the model plant Vicia faba L., toxicological assessment adopted also to determine the minimum length of the decontamination process for obtaining both the depletion of the PAH contamination and the detoxification of the soil at the end of the process. The intermediate phases of the bioremediation process were the most significant in terms of toxicity, inducing genotoxic effects and selective DNA fragmentation in the stem cell niche of the root tip. The selective DNA fragmentation can be related to the selective induction of cell death of mutant stem cells that can compromise offsprings.

ACS Style

Monica Ruffini Castiglione; Lucia Giorgetti; Simone Becarelli; Giovanna Siracusa; Roberto Lorenzi; Simona Di Gregorio. Polycyclic aromatic hydrocarbon-contaminated soils: bioaugmentation of autochthonous bacteria and toxicological assessment of the bioremediation process by means of Vicia faba L. Environmental Science and Pollution Research 2016, 23, 7930 -7941.

AMA Style

Monica Ruffini Castiglione, Lucia Giorgetti, Simone Becarelli, Giovanna Siracusa, Roberto Lorenzi, Simona Di Gregorio. Polycyclic aromatic hydrocarbon-contaminated soils: bioaugmentation of autochthonous bacteria and toxicological assessment of the bioremediation process by means of Vicia faba L. Environmental Science and Pollution Research. 2016; 23 (8):7930-7941.

Chicago/Turabian Style

Monica Ruffini Castiglione; Lucia Giorgetti; Simone Becarelli; Giovanna Siracusa; Roberto Lorenzi; Simona Di Gregorio. 2016. "Polycyclic aromatic hydrocarbon-contaminated soils: bioaugmentation of autochthonous bacteria and toxicological assessment of the bioremediation process by means of Vicia faba L." Environmental Science and Pollution Research 23, no. 8: 7930-7941.

Journal article
Published: 12 January 2016 in Environmental Science and Pollution Research
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Seven hydrocarbonoclastic new bacterial isolates were isolated from dredged sediments of a river estuary in Italy. The sediments were contaminated by shipyard activities since decades, mainly ascribable to the exploitation of diesel oil as the fuel for recreational and commercial navigation of watercrafts. The bacterial isolates were able to utilize diesel oil as sole carbon source. Their metabolic capacities were evaluated by GC-MS analysis, with reference to the depletion of both the normal and branched alkanes, the nC18 fatty acid methyl ester and the unresolved complex mixture of organic compounds. They were taxonomically identified as different species of Stenotrophomonas and Pseudomonas spp. by the combination of amplified ribosomal DNA restriction analysis (ARDRA) and repetitive sequence-based PCR (REP-PCR) analysis. The metabolic activities of interest were analyzed both in relation to the single bacterial strains and to the combination of the latter as a multibacterial species system. After 6 days of incubation in mineral medium with diesel oil as sole carbon source, the Stenotrophomonas sp. M1 strain depleted 43–46 % of Cn-alkane from C28 up to C30, 70 % of the nC18 fatty acid methyl ester and the 46 % of the unresolved complex mixture of organic compounds. On the other hand, the Pseudomonas sp. NM1 strain depleted the 76 % of the nC18 fatty acid methyl ester, the 50 % of the unresolved complex mixture of organic compounds. The bacterial multispecies system was able to completely deplete Cn-alkane from C28 up to C30 and to deplete the 95 % of the unresolved complex mixture of organic compounds. The isolates, either as single strains and as a bacterial multispecies system, were proposed as candidates for bioaugmentation in bio-based processes for the decontamination of dredged sediments.

ACS Style

Simona Di Gregorio; Giovanna Siracusa; Simone Becarelli; Lorenzo Mariotti; Alessandro Gentini; Roberto Lorenzi. Isolation and characterization of a hydrocarbonoclastic bacterial enrichment from total petroleum hydrocarbon contaminated sediments: potential candidates for bioaugmentation in bio-based processes. Environmental Science and Pollution Research 2016, 23, 10587 -10594.

AMA Style

Simona Di Gregorio, Giovanna Siracusa, Simone Becarelli, Lorenzo Mariotti, Alessandro Gentini, Roberto Lorenzi. Isolation and characterization of a hydrocarbonoclastic bacterial enrichment from total petroleum hydrocarbon contaminated sediments: potential candidates for bioaugmentation in bio-based processes. Environmental Science and Pollution Research. 2016; 23 (11):10587-10594.

Chicago/Turabian Style

Simona Di Gregorio; Giovanna Siracusa; Simone Becarelli; Lorenzo Mariotti; Alessandro Gentini; Roberto Lorenzi. 2016. "Isolation and characterization of a hydrocarbonoclastic bacterial enrichment from total petroleum hydrocarbon contaminated sediments: potential candidates for bioaugmentation in bio-based processes." Environmental Science and Pollution Research 23, no. 11: 10587-10594.

Research article
Published: 07 August 2014 in BioMed Research International
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Polycyclic aromatic hydrocarbons (PAHs) are a large group of organic contaminants causing hazards to organisms including humans. The objective of the study was to validate the vegetation of dredged sediments withPhragmites australisas an exploitable biostimulation approach to accelerate the depletion of PAHs in nitrogen spiked sediments. Vegetation withPhragmites australisresulted in being an efficient biostimulation approach for the depletion of an aged PAHs contamination (229.67±15.56 μg PAHs/g dry weight of sediment) in dredged sediments.Phragmites australisaccelerated the oxidation of the PAHs by rhizodegradation. The phytobased approach resulted in 58.47% of PAHs depletion. The effects of the treatment have been analyzed in terms of both contaminant depletion and changes in relative abundance of the metabolically active Gram positive and Gram negative PAHs degraders. The metabolically active degraders were quantified both in the sediments and in the root endospheric microbial community. Quantitative real-time PCR reactions have been performed on the retrotranscribed transcripts encoding the Gram positive and Gram negative largeαsubunit (RHDα) of the aromatic ring hydroxylating dioxygenases. The Gram positive degraders resulted in being selectively favored by vegetation withPhragmites australisand mandatory for the depletion of the six ring condensed indeno[1,2,3-cd]pyrene and benzo[g,h,i]perylene.

ACS Style

Simona Di Gregorio; Alessandro Gentini; Giovanna Siracusa; Simone Becarelli; Hassan Azaizeh; Roberto Lorenzi. Phytomediated Biostimulation of the Autochthonous Bacterial Community for the Acceleration of the Depletion of Polycyclic Aromatic Hydrocarbons in Contaminated Sediments. BioMed Research International 2014, 2014, 1 -11.

AMA Style

Simona Di Gregorio, Alessandro Gentini, Giovanna Siracusa, Simone Becarelli, Hassan Azaizeh, Roberto Lorenzi. Phytomediated Biostimulation of the Autochthonous Bacterial Community for the Acceleration of the Depletion of Polycyclic Aromatic Hydrocarbons in Contaminated Sediments. BioMed Research International. 2014; 2014 ():1-11.

Chicago/Turabian Style

Simona Di Gregorio; Alessandro Gentini; Giovanna Siracusa; Simone Becarelli; Hassan Azaizeh; Roberto Lorenzi. 2014. "Phytomediated Biostimulation of the Autochthonous Bacterial Community for the Acceleration of the Depletion of Polycyclic Aromatic Hydrocarbons in Contaminated Sediments." BioMed Research International 2014, no. : 1-11.