Genotypic variations in leaf gas exchange and yield were analysed in five upland-adapted and three lowland rice cultivars subjected to a differential soil moisture gradient, varying from well-watered to severely water-stressed conditions. A reduction in the amount of water applied resulted in a significant decrease in leaf gas exchange and, subsequently, in above-ground dry mass and grain yield, that varied among genotypes and distance from the line source. The comparison between the variable J and the Δ values in recently synthesized sugars methods, yielded congruent estimations of mesophyll conductance (g_m), confirming the reliability of these two techniques. Our data demonstrate that g_m is a major determinant of photosynthesis (A), because rice genotypes with inherently higher g_m were capable of keeping higher A in stressed conditions. Furthermore, A, g_s, and g_m of water-stressed genotypes rapidly recovered to the well-watered values upon the relief of water stress, indicating that drought did not cause any lasting metabolic limitation to photosynthesis. The comparisons between the A/C_i and corresponding A/C_c curves, measured in the genotypes that showed intrinsically higher and lower instantaneous A, confirmed this finding. Moreover, the effect of drought stress on grain yield was correlated with the effects on both A and total diffusional limitations to photosynthesis. Overall, these data indicate that genotypes which showed higher photosynthesis and conductances were also generally more productive across the entire soil moisture gradient. The analysis of Δ revealed a substantial variation of water use efficiency among the genotypes, both on the long-term (leaf pellet analysis) and short-term scale (leaf soluble sugars analysis).

The effects of the interaction between high growth temperatures and water stress on gas-exchange properties of Populus nigra saplings were investigated. Water stress was expressed as a function of soil water content (SWC) or fraction of transpirable soil water (FTSW). Isoprene emission and photosynthesis (A) did not acclimate in response to elevated temperature, whereas dark (R_n) and light (R_d) respiration underwent thermal acclimation. R_d was ∼30% lower than R_n irrespective of growth temperature and water stress level. Water stress induced a sharp decline, but not a complete inhibition, of both R_n and R_d. There was no significant effect of high growth temperature on the responses of A, stomatal conductance (g_s), isoprene emission, R_n or R_d to FTSW. High growth temperature resulted in a significant increase in the SWC endpoint. Photosynthesis was limited mainly by CO₂ acquisition in water-stressed plants. Impaired carbon metabolism became apparent only at the FTSW endpoint. Photosynthesis was restored in about a week following rewatering, indicating transient biochemical limitations. The kinetics of isoprene emission in response to FTSW confirmed that water stress uncouples the emission of isoprene from A, isoprene emission being unaffected by decreasing g_s. The different kinetics of A, respiration and isoprene emission in response to the interaction between high temperature and water stress led to rising R_d/A ratio and amount of carbon lost as isoprene. Since respiration and isoprene sensitivity are much lower than A sensitivity to water stress, temperature interactions with water stress may dominate poplar acclimatory capability and maintenance of carbon homeostasis under climate change scenarios. Furthermore, predicted temperature increases in arid environments may reduce the amount of soil water that can be extracted before plant gas exchange decreases, exacerbating the effects of water stress even if soil water availability is not directly affected.

Heat waves are predicted to increase in frequency and duration in many regions as global temperatures rise. These transient increases in temperature above normal average values will have pronounced impacts upon the photosynthetic and stomatal physiology of plants. During the summer of 2017, much of the Mediterranean experienced a severe heat wave. Here, we report photosynthetic leaf gas exchange and chlorophyll fluorescence parameters of olive (Olea europaea cv. Leccino) grown under water deficit and full irrigation over the course of the heat wave as midday temperatures rose over 40 °C in Central Italy. Heat stress induced a decline in the photosynthetic capacity of the olives consistent with reduced ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) activity. Damage to photosystem II was more apparent in plants subject to water deficit. In contrast to previous studies, higher temperatures induced reductions in stomatal conductance. Heat stress adversely affected the carbon efficiency of olive. The selection of olive varieties with enhanced tolerance to heat stress and/or strategies to mitigate the impact of higher temperatures will become increasingly important in developing sustainable agriculture in the Mediterranean as global temperatures rise.

Stomata are central players in the hydrological and carbon cycles, regulating the uptake of carbon dioxide (CO₂) for photosynthesis and transpirative loss of water (H₂O) between plants and the atmosphere. The necessity to balance water-loss and CO₂-uptake has played a key role in the evolution of plants, and is increasingly important in a hotter and drier world. The conductance of CO₂ and water vapour across the leaf surface is determined by epidermal and stomatal morphology (the number, size, and spacing of stomatal pores) and stomatal physiology (the regulation of stomatal pore aperture in response to environmental conditions). The proportion of the epidermis allocated to stomata and the evolution of amphistomaty are linked to the physiological function of stomata. Moreover, the relationship between stomatal density and [CO₂] is mediated by physiological stomatal behaviour; species with less responsive stomata to light and [CO₂] are most likely to adjust stomatal initiation. These differences in the sensitivity of the stomatal density—[CO₂] relationship between species influence the efficacy of the ‘stomatal method’ that is widely used to infer the palaeo-atmospheric [CO₂] in which fossil leaves developed. Many studies have investigated stomatal physiology or morphology in isolation, which may result in the loss of the ‘overall picture’ as these traits operate in a coordinated manner to produce distinct mechanisms for stomatal control. Consideration of the interaction between stomatal morphology and physiology is critical to our understanding of plant evolutionary history, plant responses to on-going climate change and the production of more efficient and climate-resilient food and bio-fuel crops.

This study aimed to determine whether modification of physiological parameters could be detected remotely by monitoring the spectral reflectance of olive leaves in response to different degrees of drought. Three different drought intensities were simulated: (a) a mild drought by feeding abscisic acid to detached branches; (b) a rapid and severe drought by detaching leaves and letting them dry over several hours; (c) a relatively slow drought caused by withholding water to potted olive plants. The three degrees of stress affected gas exchange and chlorophyll fluorescence. When the inhibition of photosynthesis occurred within an hour it was not accompanied by a parallel reduction in chlorophyll concentration in the carotenoid to chlorophyll ratio. Consequently, changes in spectral reflectance in the visible region, e.g. in PRI (photochemical reflectance index) and FRI (fluorescence reflectance indices) were not significantly induced. In contrast, when the inhibition of photosynthesis caused by slow developing drought was prolonged (i.e. more than 24 hours) and led to a decrease in chlorophyll concentration and to a simultaneous increase in carotenoid to chlorophyll ratio, there were significant changes in the visible region of the leaf spectral reflectance and, in turn, in PRI and FRI. We defined 16 new reference wavelengths, from visible to SWIR regions, which are sensitive to both fast‐developing and slow‐developing stresses. These reference wavelengths were used to develop an algorithm, the Relative Reflectance Increment (RRI), that was linearly related to changes in relative water content (RWC, r ² = 0.733). This algorithm showed that the 1455 nm wavelength is highly affected by drought. This wavelength was therefore used to elaborate the water content reflectance index that was inversely related to RWC (r ² = 0.702).