Cell lysis is a process of breaking cell membranes to release intracellular substances such as DNA, RNA, protein, or organelles from a cell. The detection of DNA, RNA, or protein from the lysed cells is of importance for cancer diagnostics and drug screening. In this study, we develop a microbubble array that enables the realization of multiple cell lysis induced by the shear stress resulting from the individual oscillating microbubbles. The oscillating microbubbles in the channel have similar vibration amplitudes, and the intracellular substances can be released from the individual cells efficiently. Moreover, the efficiency of cell lysis increases with increments of input voltage and sonication time. By means of DNA agarose-gel electrophoresis, a sufficient extraction amount of DNA released from the lysed cells can be detected, and there is no significant difference in lysis efficiency when compared to cell lysis achieved using commercial kits. With the advantages of the simple manufacturing process, low cost, high efficiency, and high speed, this device can serve as an efficient and versatile tool for the single-cell sequencing of cell biology research, disease diagnosis, and stem cell therapy.

Sonoporation employs ultrasound accompanied by microbubble (MB) cavitation to induce the reversible disruption of cell membranes and has been exploited as a promising intracellular macromolecular delivery strategy. Due to the damage to cells resulting from strong cavitation, it is difficult to balance efficient delivery and high survival rates. In this paper, a traveling surface acoustic wave (TSAW) device, consisting of a TSAW chip and a polydimethylsiloxane (PDMS) channel, was designed to explore single-cell sonoporation using targeted microbubbles (TMBs) in a non-cavitation regime. A TSAW was applied to precisely manipulate the movement of the TMBs attached to MDA-MB-231 cells, leading to sonoporation at a single-cell level. The impact of input voltage and the number of TMBs on cell sonoporation was investigated. In addition, the physical mechanisms of bubble cavitation or the acoustic radiation force (ARF) for cell sonoporation were analyzed. The TMBs excited by an ARF directly propelled cell membrane deformation, leading to reversible perforation in the cell membrane. When two TMBs adhered to the cell surface and the input voltage was 350 mVpp, the cell sonoporation efficiency went up to 83%.

The rapid growth in demand for portable and intelligent hardware has caused tremendous pressure on signal sampling, transfer, and storage resources. As an emerging signal acquisition technology, compressed sensing (CS) has promising application prospects in low-cost wireless sensor networks. To achieve reduced energy consumption and maintain a longer acquisition duration for high sample rate electromyogram (EMG) signals, this paper comprehensively analyzes the compressed sensing method using EMG. A fair comparison is carried out on the performances of 52 ordinary wavelet sparse bases and five widely applied reconstruction algorithms at different compression levels. The experimental results show that the db2 wavelet basis can sparse EMG signals so that the compressed EMG signals are reconstructed properly, thanks to its low percentage root mean square distortion (PRD) values at most compression ratios. In addition, the basis pursuit (BP) reconstruction algorithm can provide a more efficient reconstruction process and better reconstruction performance by comparison. The experiment records and comparative analysis screen out the suitable sparse bases and reconstruction algorithms for EMG signals, acting as prior experiments for further practical applications and also a benchmark for future academic research.