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Daniel Cruceriu
Department of Molecular Biology and Biotechnology, “Babes-Bolyai” University, 1, M. Kogălniceanu Street, 400084 Cluj-Napoca, Romania

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Journal article
Published: 13 May 2021 in Viruses
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The primary approach to controlling the spread of the pandemic SARS-CoV-2 is to diagnose and isolate the infected people quickly. Our paper aimed to investigate the efficiency and the reliability of a hierarchical pooling approach for large-scale PCR testing for SARS-CoV-2 diagnosis. To identify the best conditions for the pooling approach for SARS-CoV-2 diagnosis by RT-qPCR, we investigated four manual methods for both RNA extraction and PCR assessment targeting one or more of the RdRp, N, S, and ORF1a genes, by using two PCR devices and an automated flux for SARS-CoV-2 detection. We determined the most efficient and accurate diagnostic assay, taking into account multiple parameters. The optimal pool size calculation included the prevalence of SARS-CoV-2, the assay sensitivity of 95%, an assay specificity of 100%, and a range of pool sizes of 5 to 15 samples. Our investigation revealed that the most efficient and accurate procedure for detecting the SARS-CoV-2 has a detection limit of 2.5 copies/PCR reaction. This pooling approach proved to be efficient and accurate in detecting SARS-CoV-2 for all samples with individual quantification cycle (Cq) values lower than 35, accounting for more than 94% of all positive specimens. Our data could serve as a comprehensive practical guide for SARS-CoV-2 diagnostic centers planning to address such a pooling strategy.

ACS Style

Daniel Cruceriu; Oana Baldasici; Loredana Balacescu; Stefana Gligor-Popa; Mirela Flonta; Milena Man; Simona Visan; Catalin Vlad; Adrian Trifa; Ovidiu Balacescu; Patriciu Achimas-Cadariu. Critical Aspects Concerning the Development of a Pooling Approach for SARS-CoV-2 Diagnosis Using Large-Scale PCR Testing. Viruses 2021, 13, 902 .

AMA Style

Daniel Cruceriu, Oana Baldasici, Loredana Balacescu, Stefana Gligor-Popa, Mirela Flonta, Milena Man, Simona Visan, Catalin Vlad, Adrian Trifa, Ovidiu Balacescu, Patriciu Achimas-Cadariu. Critical Aspects Concerning the Development of a Pooling Approach for SARS-CoV-2 Diagnosis Using Large-Scale PCR Testing. Viruses. 2021; 13 (5):902.

Chicago/Turabian Style

Daniel Cruceriu; Oana Baldasici; Loredana Balacescu; Stefana Gligor-Popa; Mirela Flonta; Milena Man; Simona Visan; Catalin Vlad; Adrian Trifa; Ovidiu Balacescu; Patriciu Achimas-Cadariu. 2021. "Critical Aspects Concerning the Development of a Pooling Approach for SARS-CoV-2 Diagnosis Using Large-Scale PCR Testing." Viruses 13, no. 5: 902.

Review
Published: 03 January 2020 in Cellular Oncology
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Breast cancer is the most prevalent cancer among women worldwide and the fifth cause of death among all cancer patients. Breast cancer development is driven by genetic and epigenetic alterations, with the tumor microenvironment (TME) playing an essential role in disease progression and evolution through mechanisms like inflammation promotion. TNF-α is one of the essential pro-inflammatory cytokines found in the TME of breast cancer patients, being secreted both by stromal cells, mainly by tumor-associated macrophages, and by the cancer cells themselves. In this review, we explore the biological and clinical impact of TNF-α in all stages of breast cancer development. First of all, we explore the correlation between TNF-α expression levels at the tumor site or in plasma/serum of breast cancer patients and their respective clinical status and outcome. Secondly, we emphasize the role of TNF-α signaling in both estrogen-positive and -negative breast cancer cells. Thirdly, we underline TNF-α involvement in epithelial-to-mesenchymal transition (EMT) and metastasis of breast cancer cells, and we point out the contribution of TNF-α to the development of acquired drug resistance. Collectively, these data reveal a pro-tumorigenic role of TNF-α during breast cancer progression and metastasis. We systemize the knowledge regarding TNF-α-related therapies in breast cancer, and we explain how TNF-α may act as both a target and a drug in different breast cancer therapeutic approaches. By corroborating the known molecular effects of TNF-α signaling in breast cancer cells with the results from several preclinical and clinical trials, including TNF-α-related clinical observations, we conclude that the potential of TNF-α in breast cancer therapy promises to be of great interest.

ACS Style

Daniel Cruceriu; Oana Baldasici; Ovidiu Balacescu; Ioana Berindan-Neagoe. The dual role of tumor necrosis factor-alpha (TNF-α) in breast cancer: molecular insights and therapeutic approaches. Cellular Oncology 2020, 43, 1 -18.

AMA Style

Daniel Cruceriu, Oana Baldasici, Ovidiu Balacescu, Ioana Berindan-Neagoe. The dual role of tumor necrosis factor-alpha (TNF-α) in breast cancer: molecular insights and therapeutic approaches. Cellular Oncology. 2020; 43 (1):1-18.

Chicago/Turabian Style

Daniel Cruceriu; Oana Baldasici; Ovidiu Balacescu; Ioana Berindan-Neagoe. 2020. "The dual role of tumor necrosis factor-alpha (TNF-α) in breast cancer: molecular insights and therapeutic approaches." Cellular Oncology 43, no. 1: 1-18.

Review
Published: 22 November 2018 in International Journal of Molecular Sciences
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Colorectal cancer (CRC) is one of the most commonly diagnosed malignancies with a high incidence and mortality rate. An essential challenge in colorectal cancer management is to identify new prognostic factors that could better estimate the evolution and treatment responses of this disease. Considering their role in cancer development, progression and metastasis, miRNAs have become an important class of molecules suitable for cancer biomarkers discovery. We performed a systematic search of studies investigating the role of miRNAs in colorectal progression and liver metastasis published until October 2018. In this review, we present up-to-date information regarding the specific microRNAs involved in CRC development, considering their roles in alteration of Wnt/βcatenin, EGFR, TGFβ and TP53 signaling pathways. We also emphasize the role of miRNAs in controlling the epithelial–mesenchymal transition of CRC cells, a process responsible for liver metastasis in a circulating tumor cell-dependent manner. Furthermore, we discuss the role of miRNAs transported by CRC-derived exosomes in mediating liver metastases, by preparing the secondary pre-metastatic niche and in inducing liver carcinogenesis in a Dicer-dependent manner.

ACS Style

Ovidiu Balacescu; Daniel Sur; Calin Cainap; Simona Visan; Daniel Cruceriu; Roberta Manzat-Saplacan; Mihai-Stefan Muresan; Loredana Balacescu; Cosmin Lisencu; Alexandru Irimie. The Impact of miRNA in Colorectal Cancer Progression and Its Liver Metastases. International Journal of Molecular Sciences 2018, 19, 3711 .

AMA Style

Ovidiu Balacescu, Daniel Sur, Calin Cainap, Simona Visan, Daniel Cruceriu, Roberta Manzat-Saplacan, Mihai-Stefan Muresan, Loredana Balacescu, Cosmin Lisencu, Alexandru Irimie. The Impact of miRNA in Colorectal Cancer Progression and Its Liver Metastases. International Journal of Molecular Sciences. 2018; 19 (12):3711.

Chicago/Turabian Style

Ovidiu Balacescu; Daniel Sur; Calin Cainap; Simona Visan; Daniel Cruceriu; Roberta Manzat-Saplacan; Mihai-Stefan Muresan; Loredana Balacescu; Cosmin Lisencu; Alexandru Irimie. 2018. "The Impact of miRNA in Colorectal Cancer Progression and Its Liver Metastases." International Journal of Molecular Sciences 19, no. 12: 3711.

Preprint
Published: 29 October 2018
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Colorectal cancer (CRC) is one of the most commonly diagnosed malignancies with a high incidence and mortality. An essential challenge in colorectal cancer management is to identify new prognostic factors that could better estimate the evolution and treatment responses of this disease. Considering their role in cancer development, progression and metastasis, miRNAs have become an important class of molecules suitable for cancer biomarkers discovery. We performed a systematic search of studies investigating the role of miRNAs in colorectal progression and liver metastasis, published until October 2018. In this review, we present up-to-date information regarding the specific microRNAs involved in CRC development, considering their roles in alteration of Wnt/βcatenin, EGFR, TGFβ and TP53 signaling pathways. We also emphasize the role of miRNAs in controlling the epithelial-mesenchymal transition of CRC cells, a process responsible for liver metastasis in a circulating tumor cell-dependent manner. Furthermore, we discuss the role of miRNAs transported by CRC-derived exosomes in mediating liver metastases, by preparing the secondary pre-metastatic niche and in inducing liver carcinogenesis in a Dicer-dependent manner.

ACS Style

Ovidiu Balacescu; Daniel Sur; Calin Cainap; Simona Visan; Daniel Cruceriu; Roberta Manzat-Saplacan; Mihai-Stefan Muresan; Loredana Balacescu; Cosmin Lisencu; Alexandru Irimie. The Impact of miRNA in Colorectal Cancer Progression and Its Liver Metastases. 2018, 1 .

AMA Style

Ovidiu Balacescu, Daniel Sur, Calin Cainap, Simona Visan, Daniel Cruceriu, Roberta Manzat-Saplacan, Mihai-Stefan Muresan, Loredana Balacescu, Cosmin Lisencu, Alexandru Irimie. The Impact of miRNA in Colorectal Cancer Progression and Its Liver Metastases. . 2018; ():1.

Chicago/Turabian Style

Ovidiu Balacescu; Daniel Sur; Calin Cainap; Simona Visan; Daniel Cruceriu; Roberta Manzat-Saplacan; Mihai-Stefan Muresan; Loredana Balacescu; Cosmin Lisencu; Alexandru Irimie. 2018. "The Impact of miRNA in Colorectal Cancer Progression and Its Liver Metastases." , no. : 1.

Journal article
Published: 24 September 2018 in Palestrica Of The Third Millennium - Civilization And Sport
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ACS Style

Laura Ioana Gavrilaș; Corina Ionescu; Ovidiu Bălăcescu; Daniel Cruceriu; Lorena Filip; Doina Miere. Physical activity in colorectal cancer. Palestrica Of The Third Millennium - Civilization And Sport 2018, 13, 181 -186.

AMA Style

Laura Ioana Gavrilaș, Corina Ionescu, Ovidiu Bălăcescu, Daniel Cruceriu, Lorena Filip, Doina Miere. Physical activity in colorectal cancer. Palestrica Of The Third Millennium - Civilization And Sport. 2018; 13 (3):181-186.

Chicago/Turabian Style

Laura Ioana Gavrilaș; Corina Ionescu; Ovidiu Bălăcescu; Daniel Cruceriu; Lorena Filip; Doina Miere. 2018. "Physical activity in colorectal cancer." Palestrica Of The Third Millennium - Civilization And Sport 13, no. 3: 181-186.