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Mrs. Lamiaa Mahmoud
University of Florida

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0 Biotechnology
0 Climate Change
0 Physiology
0 Salinity
0 HLB

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Journal article
Published: 14 July 2021 in Plants
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Huanglongbing (HLB), caused by the phloem-limited bacterium Candidatus Liberibacter asiaticus (CaLas), is the primary biotic stress causing significant economic damage to the global citrus industry. Among the abiotic stresses, salinity affects citrus production worldwide, especially in arid and coastal regions. In this study, we evaluated open-pollinated seedlings of the S10 (a diploid rootstock produced from a cross between two siblings of the Hirado Buntan Pink pummelo (Citrus maxima (Burm.) Merr.) with the Shekwasha mandarin (Citrus reticulata Blanco)) for their ability to tolerate HLB and salinity stresses. In a greenhouse study, ‘Valencia’ sweet orange (either HLB-positive or negative) was grafted onto six clonally propagated lines generated from the screened seedlings in the greenhouse and the trees were irrigated with 150 mM NaCl after eight months of successful grafting and detection of CaLas in the leaf petioles. Cleopatra mandarin was used as a salt-tolerant and HLB-sensitive rootstock control. CaLas infection was monitored using a quantitative polymerase chain reaction before and after NaCl treatments. Following three months of NaCl treatment, ‘Valencia’ leaves on the S10 rootstock seedlings recorded lower levels of chlorophyll content compared to Cleopatra under similar conditions. Malondialdehyde content was higher in HLB-infected ‘Valencia’ grafted onto Cleopatra than in the S10 lines. Several plant defense-related genes were significantly upregulated in the S10 lines. Antioxidant and Na+ co-transporter genes were differentially regulated in these lines. Based on our results, selected S10 lines have potential as salt-tolerant rootstocks of ‘Valencia’ sweet orange under endemic HLB conditions. However, it is necessary to propagate selected lines through tissue culture or cuttings because of the high percentage of zygotic seedlings derived from S10.

ACS Style

Lamiaa Mahmoud; Patrick Huyck; Christopher Vincent; Frederick Gmitter; Jude Grosser; Manjul Dutt. Physiological Responses and Gene Expression Patterns in Open-Pollinated Seedlings of a Pummelo-Mandarin Hybrid Rootstock Exposed to Salt Stress and Huanglongbing. Plants 2021, 10, 1439 .

AMA Style

Lamiaa Mahmoud, Patrick Huyck, Christopher Vincent, Frederick Gmitter, Jude Grosser, Manjul Dutt. Physiological Responses and Gene Expression Patterns in Open-Pollinated Seedlings of a Pummelo-Mandarin Hybrid Rootstock Exposed to Salt Stress and Huanglongbing. Plants. 2021; 10 (7):1439.

Chicago/Turabian Style

Lamiaa Mahmoud; Patrick Huyck; Christopher Vincent; Frederick Gmitter; Jude Grosser; Manjul Dutt. 2021. "Physiological Responses and Gene Expression Patterns in Open-Pollinated Seedlings of a Pummelo-Mandarin Hybrid Rootstock Exposed to Salt Stress and Huanglongbing." Plants 10, no. 7: 1439.

Journal article
Published: 25 November 2020 in Horticulturae
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Our study aimed to evaluate the physiological responses following salinity treatment of three putatively salt-tolerant Citrus rootstocks recently developed by the University of Florida’s Citrus breeding program. Four-month-old seedlings from each of the three rootstocks (HS1, HS17, and HC15) were irrigated with 0, 60, 80, and 100 mm NaCl solution. The seedlings were evaluated together with the salt-tolerant Cleopatra mandarin as a positive control, Volkamer lemon as a moderately salt-tolerant rootstock, and the salt-sensitive Carrizo rootstock as a negative control. Our results demonstrated that chlorophyll content, net CO2 assimilation rate (A), transpiration rate (E), and stomatal conductance (gsw ) significantly decreased in response to salinity. Na+ and Cl− levels were higher in leaf tissues than in the roots. Relatively little damage to the cellular membrane was recorded in HC15 and Cleopatra rootstocks under the 100 mm NaCl treatment, along with high accumulation of total phenolic content (TPC), while HS17 had the highest proline levels. Our results indicate that HC15 and HS17 rootstocks exhibited salt tolerance capacity via different strategies under salt stress and could be suitable replacements to the commercially available, salt-tolerant Cleopatra rootstock.

ACS Style

Lamiaa Mahmoud; Manjul Dutt; Christopher Vincent; Jude Grosser. Salinity-Induced Physiological Responses of Three Putative Salt Tolerant Citrus Rootstocks. Horticulturae 2020, 6, 90 .

AMA Style

Lamiaa Mahmoud, Manjul Dutt, Christopher Vincent, Jude Grosser. Salinity-Induced Physiological Responses of Three Putative Salt Tolerant Citrus Rootstocks. Horticulturae. 2020; 6 (4):90.

Chicago/Turabian Style

Lamiaa Mahmoud; Manjul Dutt; Christopher Vincent; Jude Grosser. 2020. "Salinity-Induced Physiological Responses of Three Putative Salt Tolerant Citrus Rootstocks." Horticulturae 6, no. 4: 90.

Review
Published: 13 December 2019 in Plants
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Horticultural crops, including fruit, vegetable, and ornamental plants are an important component of the agriculture production systems and play an important role in sustaining human life. With a steady growth in the world’s population and the consequent need for more food, sustainable and increased fruit and vegetable crop production is a major challenge to guarantee future food security. Although conventional breeding techniques have significantly contributed to the development of important varieties, new approaches are required to further improve horticultural crop production. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) has emerged as a valuable genome-editing tool able to change DNA sequences at precisely chosen loci. The CRISPR/Cas9 system was developed based on the bacterial adaptive immune system and comprises of an endonuclease guided by one or more single-guide RNAs to generate double-strand breaks. These breaks can then be repaired by the natural cellular repair mechanisms, during which genetic mutations are introduced. In a short time, the CRISPR/Cas9 system has become a popular genome-editing technique, with numerous examples of gene mutation and transcriptional regulation control in both model and crop plants. In this review, various aspects of the CRISPR/Cas9 system are explored, including a general presentation of the function of the CRISPR/Cas9 system in bacteria and its practical application as a biotechnological tool for editing plant genomes, particularly in horticultural crops.

ACS Style

Lígia Erpen-Dalla Corte; Lamiaa M. Mahmoud; Tatiana S. Moraes; Zhonglin Mou; Jude W. Grosser; Manjul Dutt. Development of Improved Fruit, Vegetable, and Ornamental Crops Using the CRISPR/Cas9 Genome Editing Technique. Plants 2019, 8, 601 .

AMA Style

Lígia Erpen-Dalla Corte, Lamiaa M. Mahmoud, Tatiana S. Moraes, Zhonglin Mou, Jude W. Grosser, Manjul Dutt. Development of Improved Fruit, Vegetable, and Ornamental Crops Using the CRISPR/Cas9 Genome Editing Technique. Plants. 2019; 8 (12):601.

Chicago/Turabian Style

Lígia Erpen-Dalla Corte; Lamiaa M. Mahmoud; Tatiana S. Moraes; Zhonglin Mou; Jude W. Grosser; Manjul Dutt. 2019. "Development of Improved Fruit, Vegetable, and Ornamental Crops Using the CRISPR/Cas9 Genome Editing Technique." Plants 8, no. 12: 601.