We report a strategy to fabricate a rapid and stable surface-enhanced Raman scattering (SERS)-based hybrid nanomaterial using gold nanopopcorns attached single-walled carbon nanotubes (AuNP@f₃-SWCNTs) for label-free detection and photothermal killing of bacteria. Herein, previously ester-functionalized single-walled carbon nanotubes (f₁-SWCNTs) undergo 1,3-dipolar cycloaddition reaction with in situ generated nitrile imine under Microwave (MW) irradiation to form a doubly ester terminated SWCNT cycloadduct (f₂-SWCNT). The ester terminals are further modified with 4-aminothiophenol (4-ATP) under MW-irradiation to form thiol-terminated SWCNT templates (f₃-SWCNTs) that allow gold nanopopcorns (AuNPs) to covalently and uniformly attach at a minimum inter-particle distance thus yielding a hybrid nanomaterial (AuNP@f₃-SWCNT) with good aqueous stability and abundant ‘hotspots’. Consequently, monoclonal E. coli antibody-conjugated bioassays fabricated with our AuNP@f₃-SWCNT substrates (mAb-AuNP@f₃-SWCNT) rapidly detect E. coli in water with good selectivity and impressive SERS sensitivity. The detection limit of E. coli 49979, selected as a model to establish proof of principle, was found to be 1.0 × 10² CFU mL⁻¹. Furthermore, the AuNP@f₃-SWCNT hybrid nanomaterial offers impressive photothermal pathogen killing effects. The synergy-type enhancement effect arising from the inherent noble properties of the respective components of the hybrid nanomaterial indicate that our AuNP@f₃-SWCNT has the potential for further application in multiplex detection in samples.

Monoclonal antibody-conjugated sphere-shaped gold nanoparticles were combined with single-walled carbon nanotubes (SWCNTs) to create a nanohybrid system to selectively detect and eradicate multiple drug resistant Salmonella (MDRS) typhimurium DT104 bacteria. The Raman signal intensity from Rhodamine 6G (Rh6G) modified monoclonal AC04 antibody SWCNTs–gold nanoparticle (SWCNT–GNPs) hybrid provided a SERS enhancement by several orders of magnitude to detect the MDRS bacteria over the GNP system. A targeted photothermolysis experiment using 670 nm light at 2 W cm⁻² for 15 min, resulted in selective and irreparable damage to more than 99% Salmonella DT104 at the concentration of 10⁵ CFU mL⁻¹. In comparison to solely SWCNTs or GNPs, our SWCNT–GNPs nanohybrids have also shown a better photothermal efficiency.