The final effluents of three (Alice, Dimbaza, and East London) wastewater treatment plants (WWTPs) were evaluated to determine their physicochemical quality and prevalence of multiple antibiotics resistant (MAR) Pseudomonas species, between August 2007 and July 2008. The annual mean total Pseudomonas count (TPC) was 1.20 × 10⁴ (cfu/100 mL), 1.08 × 10⁴ (cfu/100 mL), and 2.66 × 10⁴ (cfu/100 mL), for the Alice, Dimbaza, and East London WWTPs respectively. The effluents were generally compliant with recommended limits for pH, temperature, TDS, DO, nitrite and nitrate; but fell short of target standards for turbidity, COD, and phosphate. The tested isolates were highly sensitive to gentamicin (100%), ofloxacin (100%), clindamycin (90%), erythromycin (90%) and nitrofurantoin (80%); whereas high resistance was observed against the penicillins (90–100%), rifampin (90%), sulphamethoxazole (90%) and the cephems (70%). MAR index ranged between 0.26 and 0.58. The study demonstrated that MAR Pseudomonas species were quite prevalent in the final effluents of WWTPs in South Africa; and this can lead to serious health risk for communities that depend on the effluent-receiving waters for sundry purposes.

Distribution, investigation, surveillance and control (DISC) of cholera outbreaks in endemic/non-endemic regions has been a concerted approach towards the management of the causal pathogen. Relevant organization, government, health systems and the public have implemented several steps towards controlling the menace, yet pathogen continues to occur with diverse phenotypes/genotypes of high clinical and epidemiological relevance. The study determines antibiotic susceptibility/resistance pattern of Vibrio cholerae isolates retrieved from six domestic water sources between March and August 2018. Serological and molecular typing methods (polymerase chain reaction or PCR) were used to confirm the isolates identity. Antibiotic susceptibility testing was conducted using six commonly employed antibiotics of V. cholerae according to the recommendation of Clinical Laboratory Standard and European Committee for Antimicrobial Susceptibility Testing with other relevant antibiotics of investigative epidemiology and infection control, employing both disc diffusion test and PCR gene detection. Samples presumptive counts ranged between 1.10 to 7.91 log10 CFU/mL. Amongst the 759 presumptive isolates retrieved, sixty-one were confirmed as V. cholerae which were further serogrouped as Non-O1/Non-O139 V. cholerae. Various V. cholerae resistant phenotypes/genoytypes were detected vis: carbapenemase (CR-Vc; 31.1%/5.3%). New Delhi Metallobetalactamase (NDM-1-Vc; 23.0%/42.5%), extended spectrum betalactamase (ESBL-Vc; 42.6%/blaTEM:86,7%), chloramphenicol resistance (62.3%/Flor: 46.2%}, tetracycline resistance (70.5%/46.7%), AmpC resistance (21.0 (34.4%/56.7%)) and various other resistant genotypes/phenotypes. It was observed that more than 50% of the confirmed V. cholerae isolates possess resistance to two or more antibiotic classes/groups with multiple antibiotic resistance index (MARI) ranging from 0.031 to 0.5. This observation provides necessary information and updates for surveillance, planning and implementation of control strategies for cholera. It would also encourage decision making, formulation of policy by the government and cholera control authorities.

Antibiotic resistance genes (ARGs) and Mobile genetic elements (MGEs) are major global emerging pollutants of the environment and water nexus which various investigators of related studies have reported. Observing ARGs and MGEs in water bodies, wastewater treatment systems, and estuaries is indicative of relevant risk, resistant bacteria/ARGs spread or potential health concern and may result environmental pathogen bloom if appropriate research-based strategies are not implemented to remove these lethal genetic materials. Despite reports and knowledge-based strategies for removal, the challenge yet persists. This study aims to appraise the impact/contribution of related studies and emphasize the necessity for applying combined research-based/practitioners approach in addressing the expanding challenge of ARGs/MGEs in wastewater/waterbodies. The study describes a bibliometric assessment of antibiotic resistance determinants annual scientific publications on the Web of Science, an annual growth rate of related articles, top articles per citations with search topics and content-review analysis to evaluate the methods of removal of ARGs/MGEs. A total of 1301 articles of wastewater treatment systems were retrieved with date range of 1997–2019. A description of the study Annual Growth Rate of 37.82% at R² of 0.7863 was observed with an increasing article publication and a decreasing total citation rate indicating persistent reports of dispersion on ARGs/MGEs studies in the water bodies and environment. Although there abound extensive studies and reports of ARGs and MGEs in water nexus and wastewater release with research based removal strategies, the impact of such reports have not been fully actualized amongst wastewater system practitioners. A lucid drive towards implementing ARGs/MGEs removal strategies from the environment by applying affirmed research-based methods are suggestive.

Introduction and purpose: The recent cholera spread, new cases, and fatality continue to arouse concern in public health systems; however, interventions on control is at its peak yet statistics show continuous report. This study characterized atypical and patho-significant environmentalVibrio choleraeretrieved from ground/surface/domestic water in rural-urban-sub-urban locations of Amathole District municipality and Chris Hani District municipality, Eastern Cape Province, South Africa.Methods: Domestic/surface water was sampled and 759 presumptiveV. choleraeisolates were retrieved using standard microbiological methods. Virulence phenotypic test: toxin co-regulated pili (tcp), choleragen red, protease production, lecithinase production, and lipase test were conducted. Serotyping using polyvalent antisera (Bengal and Ogawa/Inaba/Hikojima) and molecular typing: 16SrRNA, OmpW, serogroup (Vc-O1/O139), biotype (tcpAClas/El Tor,HlyAClas/El Tor,rstRClas/El Tor,RS1,rtxA,rtxC), and virulence (ctxA,ctxB,zot,ace,cep,prt,toxR,hlyA) genes were targeted.Result: Result of16SrRNAtyping confirmed 508 (66.9%) whileOmpWdetected/confirmed 61 (12.01%)V. choleraestrains. Phenotypic-biotyping scheme showed positive test to polymyxin B (68.9%), Voges proskauer (6.6%), and Bengal serology (11.5%). Whereas Vc-O1/O139 was negative, yet two of the isolates harbored the cholera toxin with a gene-typectxBandhlyAClas: 2/61, revealing atypical/unusual/dual biotype phenotypic/genotypic features. Other potential atypical genotypes detected includerstR: 7/61,Cep: 15/61,ace: 20/61,hlyAElTor: 53/61,rtxA: 30/61,rtxC: 11/61, andprtV: 15/61 respectively.Conclusion: Although additional patho-significant/virulent genotypes associated with epidemic/sporadic cholera cases were detected, an advanced, bioinformatics, and post-molecular evaluation is necessary. Such stride possesses potential to adequately minimize future cholera cases associated with dynamic/atypical environmentalV. choleraestrains.